Catalysts
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2021
·Vol. 11
·期 10
·1214
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IF 4.146
Weilai Fu,Qiang Wang,Shuhui Chen,Yunshuang Wang,Yaru Wang,Peifeng Duan,Ganfeng Yi,Chao Liu,Xian Zhang,Zhiming Rao
Nitrogen pollution in water bodies is becoming increasingly serious, and how to remove nitrogen from water bodies economically and effectively has become a research hotspot. Especially in recent years, with the gradual expansion of aquaculture in China, the content of nitrite and other nitrogen-containing substances in water bodies has been increasing, which inhibits the growth of farm animals and is one of the causes of eutrophication in water bodies. In this study, a strain of bacteria was isolated from the sludge of an aquaculture fishpond and identified asPseudomonas stutzeri, which can efficiently degrade nitrite. After continuous domestication in nitrite mixed solution, the nitrite nitrogen reduction capacity ofP. stutzeriwas significantly improved. Univariate experiments aiming to optimize the degradation conditions indicate that the optimal culture conditions for strain F2 are: medium with a carbon source of sodium succinate; C/N of 18; pH of 8; culture temperature of 28 °C; and shaking speed of 210 rpm in the shaker. Under the optimal culture conditions, the NO2−-N concentration of the culture solution was 300 mg/L, and the nitrite removal rate reached 98.67%. Meanwhile, the results of the nitrogen balance test showed that the strain converted 6.1% of the initial nitrogen into cellular organic nitrogen and 62.3% into gaseous nitrogen.Keywords:nitrogen pollution;nitrite removal;domestication;Pseudomonas stutzeri
研究领域:
环境科学
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微生物学
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DOI
高影响因子
BIORESOURCE TECHNOLOGY
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2021
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IF 9.642
Yibo Zhang,Jing Wan,Zhang Li,Zhenbing Wu,Chenyuan Dang,Jie Fu
This study utilized sulfamethoxazole (SMX) acclimatization to enhance the tolerance and biodegradation capacity of Chlorella vulgaris . Compared to wild C. vulgaris , the growth inhibition and oxidative damage induced by SMX evidently decreased in acclimated C. vulgaris , and meanwhile photosynthetic and antioxidant activities were significantly promoted. The physiological analyses with the aid of principal component analysis revealed the increase of catalase and glutathione reductase activities was the critical tolerant mechanism of acclimated C. vulgaris . As the consequence, the acclimated C. vulgaris exhibited enhanced efficiency and (pseudo-first-order) kinetic rate for removal of SMX. The distribution analysis of residual SMX demonstrated the biodegradation was the major removal mechanism of SMX by C. vulgaris , while bioadsorption and bioaccumulation made pimping contributions. During the degradation process of SMX, nine transformation products (TPs) were identified. Based on the identified TPs, a possible transformation pathway was proposed.
研究领域:
环境科学
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微生物学
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生物技术
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产品关联
DOI
PubMed
高影响因子
Journal of Water Process Engineering
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2025
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IF 6.7
Caiyun Zhang,Jin Qian,Jiajie Wang,Xinyan Zhang,Zhuqiu Sun,Bairen Yang
Herein, a microbial consortia directed domestication approach based on degradation pathways was proposed. This approach employs intermediates capable of promoting ring-opening and dechlorination of m -dichlorobenzene ( m -DCB), in conjunction with m -DCB itself, as substrates for stepwise domestication, with the aim of overcoming the limitation of microbial activity due to the high toxicity of m -DCB. The microbial community analysis for the directed domestication of consortia domesticated that microorganisms responsible for ring-opening and dechlorination were predominant. Meanwhile, the removal efficiency (RE) of m -DCB by directed domestication microbial consortia was significantly higher than the original microbial consortia and abiotic control. To further improve the ring-opening and dechlorination performance of m -DCB, electrical stimulation was introduced as a means of enhancing the directed domestication. Performance tests showed that the highest RE and elimination capacity (EC) of m -DCB were 95.23 % and 23.77 mg/m 3 /h, at an electrical stimulation of 0.7 V, respectively. The microbial community analyses revealed that electrical stimulation led to an increase in the relative abundance of key functional genera (e.g., Rhodanobacter and Pseudomonas ) and functional genes (e.g., catE and dhaA), thereby facilitating the oxidation ring-opening and reductive dechlorination of m -DCB. Metagenomic sequencing further revealed the potential and mechanisms for enhanced biodegradation of m -DCB by the coupled approach of electrical stimulation and directed domestication. The study demonstrates that the biodegradation potential of m -DCB can be enhanced through electrical stimulation based on directed domestication, providing a strategic pathway for microbial construction and improved m -DCB removal from exhaust gases.
研究领域:
环境科学
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微生物学
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DOI
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
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2022
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IF 14.224
Rui Chen,Lin Hu,Han Zhang,Dachao Lin,Jinlong Wang,Daliang Xu,Weijia Gong,Heng Liang
The occurrence of emerging contaminants is attracting widespread attention due to its potential threat to aquatic organisms and public health. Gravity-driven membrane (GDM) filtration can effectively eliminate water contamination with pathogenic microorganisms in rural areas while being challenged by various micro-pollutants (MPs). This study investigated the removal and transformation of sulfamethoxazole (SMX) in GDM for the first time, and pre-adding acclimated activated sludge was proposed to improve the removal of MPs. More rapid and higher SMX degradation was observed with pre-adding domestication sludge, and the system had better tolerance to SMX shocks. Besides, in the presence of domesticated sludge, more SMX metabolic pathways and better mineralization rates were obtained, which was related to more SMX-resistant bacteria and easier biodegradable carbon sources in the system. Pre-adding sludge also increased the richness and diversity of bacterial community, which provided higher removal efficiencies of conventional pollutants. Thus, the removal rates of DOC (14.7%), NH 4 + -N (5.6%) and fluorescent substances were obviously improved compared with the control group. In this study, the crisis of MPs was tackled and the removal of conventional pollutants was enhanced by pre-adding domesticated sludge in GDM, which ensured the water quality in rural areas.
研究领域:
环境科学
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水处理工程
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微生物学
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DOI
PubMed
高影响因子
MARINE POLLUTION BULLETIN
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2021
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IF 5.553
Jun Mu,Qingxue Leng,Guangfeng Yang,Baikang Zhu
Recurring oil spill accidents have been a global challenge and contribute to PAHs' heavy accumulation in marine sediments. The rapid bioremediation of PAHs with high concentrations in marine sediments has rarely been studied. In this study, four representative PAHs in crude oil were tested for fast anaerobic biodegradation. An efficient system for the anaerobic degradation of high-concentration PAHs was obtained using petroleum-acclimated marine sediments as inoculants in the treatment system. The degradation efficiencies of benzo[ b ]fluoranthene, benzo[ a ]pyrene, pyrene, and phenanthrene reached 0.21, 1.71, 3.89, and 4.10 mg/(L·d), respectively, which are 16, 2.8, 1.8, and 1.0 times higher than the reported values. Nitrate was preferred to sulfate as an electron acceptor. The acclimated sediment contains a high abundance of hydrocarbon-degrading bacteria. The number and diversity of species in the treatment system supplemented with PAHs decreased, but the abundance of some hydrocarbon-degrading bacteria and hydrocarbon-intermediate utilising bacteria increased, and ecological succession was observed.
研究领域:
环境科学
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微生物学
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生物修复
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海洋生态学
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DOI
PubMed
高影响因子
CHEMOSPHERE
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2023
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IF 8.8
Xuan Zhang,Keke Geng,Ningning Wu,Gang Hu,Ben Fan,Jian He,Wenjing Qiao
4-Chloro-2-methylphenoxyacetic acid (MCPA) is a widely used herbicide across the world. MCPA is persistent and easily transports into anoxic environment, such as groundwater, sediments and deep soils. However, little research on anaerobic microbial degradation of MCPA was carried out. The functional microorganisms as well as the catabolic pathway are still unknown. In this research, an anaerobic MCPA-degrading bacterial consortium was enriched from the river sediment near a pesticide-manufacturing plant. After about 6 months’ acclimation , the MCPA transformation rate of the consortium reached 4.32 μmol g −1 day −1 , 25 times faster than that of the original sludge . 96% of added MCPA (2.5 mM) was degraded within 9 d of incubation. Three metabolites including 4-chloro-2-methylphenol (MCP), 2-methylphenol (2-MP) and phenol were identified during the anaerobic degradation of MCPA. An anaerobic catabolic pathway was firstly proposed: firstly, MCPA was transformed to MCP via the cleavage of the aryl ether, then MCP was reductively dechlorinated to 2-MP which was further demethylated to phenol. The 16S rRNA gene amplicon sequencing revealed a substantial shift in the bacterial community composition after the acclimation. SBR1031, Acidaminococcaceae , Aminicenantales , Syntrophorhabdus , Acidaminobacter , Bacteroidetes_vadinHA17 , Methanosaeta , Bathyarchaeia , KD4-96, Anaeromyxobacter , and Dehalobacter were significantly increased in the enriched consortium after acclimation, and positively correlated with the anaerobic degradation of MCPA as suggested by heat map correlation analysis. This study provides a basis for further elucidation of the anaerobic catabolism of MCPA, and contributes to developing efficient and low-cost anaerobic treatment technologies for MCPA pollution.
研究领域:
环境微生物学
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生物降解与生物转化
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水污染控制
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产品关联
DOI
PubMed
高影响因子
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