Science of Advanced Materials
·
2020
·Vol. 12
·期 2
·304-311
·
IF 1.117
Xu Gaofeng,Jia Chengxinzhuo,Shi Zhengjun,Liang Ruijuan,Wu Chunhua,Liu Hu,Yan Chao,Fan Jincheng,Hou Hua,Ding Tao,Guo Zhanhu
The ex-situ catalytic cracking of camelina oil using nickel loaded MCM-41 as catalyst at 450 °C in fixed bed reactor was studied. Results revealed that the yield, selectivity and chemical composition of the liquid products was improved by nickel loaded MCM-41 without affecting
the crystalline structure of MCM-41. Moreover, the loaded nickel onto MCM-41 facilitated the cyclization, alkylation, aromatization, deoxygenation, isomerization and cracking reactions.
研究领域:
化学工程
·
能源科学
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DOI
高影响因子
CHEMICAL ENGINEERING & TECHNOLOGY
·
2019
·Vol. 43
·期 3
·403-411
·
IF 2.418
Huan Li,Tian Ma,Zhensheng Shen,An Li,Jie Yang,Dong Xiang,Jiuyi Liu,Shuguang Deng,Yingqiang Sun
1-Butyl-3-methylimidazolium hydrogen sulfate ([Bmim][HSO 4 ]) is utilized to catalyze transesterification of camelina oil with methanol. The major compositions of camelina biodiesel are saturated fatty acid esters (C16:0, C18:0), monounsaturated, and polyunsaturated fatty acid esters (C18:2, C18:3). The effects of reaction temperature, reaction time, M methanol : M Camelina oil , and M [Bmim][HSO4] : M Camelina oil on biodiesel production are investigated in detail, and a general mathematical model is developed to well predict the biodiesel yield. Also, [Bmim][HSO 4 ] is thermally stable to recycle for four times with a high biodiesel yield. The fuel properties of camelina biodiesel are all comparable to the American Society for Testing Materials (ASTM) standards.
研究领域:
能源科学
·
化学工程
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DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2021
·
IF 6.953
Zhenquan Ye,Tianyu Li,Degang Qing,Yu Sun,Haiyun Chen,Qian Yu,Chunyan Yan
Alhagi pseudalhagi , commonly known as camel thorn, is used as an indigenous medicinal plant in China. The present study was designed to elucidate the structure of a novel polysaccharide, APP90-2, isolated from Alhagi pseudalhagi and evaluate its osteogenic activity. A homogeneous polysaccharide (APP90-2) was obtained from A. pseudalhagi via DEAE-52 and Sephacryl S-100 columns, with a molecular weight of 5.9 kDa. Monosaccharide, GC–MS, and NMR analyses showed that APP90-2 consisted of α - l -Rha p -(1→, →3)- α - l -Ara f -(1→, →5)- α - l -Ara f -(1→, →4)- β - d -Xyl p -(1→, α - d -Glc p -(1→, →3,5)- α - l -Ara f -(1→, →4)- β - d -GlcA p -(1→, →4)-3-OAc- α - d -Glc p -(1→, →3)- α - d -Gal p -(1→, →3)- β - d -GalA p -(1→, →4)- α - d -Gal p -(1→, →6)- α - d -Man p -(1→, →4,6)- β - d -Gal p -(1→, and →3,6)- β - d -Glc p -(1→ with relative molar ratios of 4.1:1.8:6.1:6.7:1.7:1.0:1.5:2.7:2.4:1.1:2.3:2.6:1.4:2.0. Morphological analyses revealed that APP90-2 interacted with Congo-red and had an obvious honeycomb structure. Additionally, APP90-2 significantly promoted proliferation, differentiation, and mineralization of MC3T3-E1 cells, indicating that APP90-2 exhibited pronounced osteogenic activity. Therefore, our findings suggest that A. pseudalhagi may be used as an alternative medicine or health supplement for the prevention and treatment of osteoporosis.
研究领域:
药理学
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生物化学
·
分子生物学
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DOI
PubMed
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2024
·
IF 6
Qi Hao,Yongdong Lei,Runze Li,Lingzhen Ma,Huihui Zheng,Xiaorong Deng,Jian Zhang
Pretreatment for dairy processing sometimes requires freezing and thawing to ensure a steady supply of fresh raw milk. On this basis, a series of studies were conducted to ascertain the impact of freeze-thaw cycles on the physicochemical stability and nutritional quality of camel milk. The findings demonstrated that while the lipid content and apparent viscosity of camel milk were significantly reduced, the acidity and quantity of short- and medium-chain free fatty acids were enhanced by repeated freeze‒thaw cycles. Freeze‒thaw cycles have been demonstrated to result in an increase in protein oxidation in camel milk, which in turn has been observed to give rise to a shift in the secondary structure towards random coils. The particle size distribution of camel milk exhibited aggregation of the proteins, which consequently reduced the physical stability of the camel milk. The results of this study may help to deepen our understanding of the quality characteristics of raw camel milk materials while providing methodological references and scientific support for the storage of raw camel milk.
研究领域:
食品科学
·
营养学
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产品关联
DOI
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2019
·
IF 3.714
Ali Mohsin,Hui Ni,Yanxia Luo,Yanlong Wei,Xiwei Tian,Wenyan Guan,Muhammad Ali,Imran Mahmood Khan,Sobia Niazi,Salim-ur Rehman,Yingping Zhuang,Meijin Guo
Camel milk is worldwide known for its profound nutritional value . However, the poor curd formation of camel milk renders low consumer acceptability. In current study, xanthan gum was added in the production process to attain an adequate firmness factor. Further for enhancing consumer acceptability, dates paste was also added. Storage study was performed four times after an elapse of every 7 days to confirm the product quality for corresponding fractions (0.25, 0.5 and 0.75%) of biosynthesized xanthan (BX) and 1% gelatin as control. Moreover, the rheological measurements were performed and the experimental data were assessed by Herschel-Bulkley model. With the addition of BX, the camel milk date yoghurt (CMDY) demonstrated the shear-thinning behavior with pseudoplastic fluid characteristics. The rheological properties of CMDY were significantly improved in correspondence to higher concentration of BX. Relatively, 0.75% of BX addition resulted in the best texture of yoghurt and obtained an average firmness of 445 ± 34.11 g. Furthermore, confocal CSLM and SEM micrographs demonstrated the detail microstructure of all yoghurt samples. Specifically, addition of 0.75% BX in yoghurt sample demonstrated a strengthened (dense) network of casein micelles. Lastly, the addition of 0.75% BX along with 10% dates paste significantly enhanced the sensory characteristics of CMDY.
研究领域:
食品科学
·
微生物学
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DOI
高影响因子
APPLIED BIOCHEMISTRY AND MICROBIOLOGY
·
2022
·Vol. 58
·期 6
·796-805
·
IF 1.065
Li P.,Jiang Y.,Fei Y. Y.,Zeng X. C.
Abstract Benzo(a)pyrene (BaP) is one of the most common organic pollutants in the environment, and its microbial removal has great ecological and economic value. Here, using BaP (50 mg/L) as the sole carbon source, dynamic changes in camel rumen and gut bacterial communities were analyzed by high-throughput sequencing. Next, cultivable BaP-degrading isolates were obtained, and their degradation characteristics were explored. The relative abundance of most bacteria in the gut and rumen underwent apparent changes after BaP treatment. The rumen and gut bacteria have the ability to degrade BaP but fluctuated during iterative culturing from the first to the fifth generation of gut communities displaying the highest BaP degradation efficiency (BDE) (74.4 and 81.1%, respectively). Moreover, 37 isolates exhibiting BDE were identified. Among these isolates, CL9 ( Klebsiella sp.), CL16 ( Ochrobactrum sp.), CC6 ( Ochrobactrum sp.), and CC16 ( Bacillus sp.) showed BDEs of up to 70.8, 69.3, 68.4, and 76.2%, respectively. The enzyme catechol-2,3-dioxygenase was involved in BaP degradation in these four BaP-degrading isolates and represented the main BaP degradation pathway in isolates CL9 and CL16; however, the relative expression level of the gene phnAc was higher in isolates CC6 and CC16 than in isolates CL9 and CL16. Our results provided promising bacterial resources for the removal of BaP and laid the foundation for the application of animal gastrointestinal bacteria to degrade environmental pollutants.
研究领域:
环境微生物学
·
动物科学
·
生物修复
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DOI
高影响因子
Nutrients
·
2025
·Vol. 17
·期 10
·1693
·
IF 5
Binsong Han,Yuhui Ye,Cunzheng Zhang,Lina Zhang,Peng Zhou
Background: Camel milk is considered to be an important source of bioactive peptides with potential anti-diabetic effects. However, the mechanism by which these active peptides exert their anti-diabetic effects is not clear. The aim of this study was to systematically evaluate the in vivo anti-diabetic effects of Leucine-Leucine-Proline-Lysine (LLPK), a novel dipeptidyl peptidase-4 (DPP-4) inhibitory peptide identified from the in vitro gastrointestinal digestion product of camel milk.Methods: Adb/dbdiabetic mouse model was used, and LLPK was administered to mice at doses of 50 mg/kg BW and 100 mg/kg BW as a daily oral gavage for 30 days. The effects of LLPK on fasting blood glucose (FBG), oral glucose tolerance test (OGTT), insulin tolerance test (ITT), and serum lipid levels were monitored, and possible mechanisms of action were elucidated using proteomics.Results: The results demonstrated that LLPK significantly improved diabetic symptoms, including FBG, OGTT, ITT, and serum lipid levels indb/dbdiabetic mice. Furthermore, significantly increased levels of serum glucagon-like peptide 1 (GLP-1) and reduced serum DPP-4 activity were observed in the LLPK-treated group compared to the control group. Hepatic proteomics indicated that LLPK improved glucose and lipid metabolism via the PPAR signaling pathway, where the key targets were Scd1, Acox1, Acaa1b, Slc27a1, Acsl1, and Ehhadh.Conclusions: In summary, this study provided new insights into the anti-diabetic mechanisms of camel milk and supported the development of camel milk-based anti-diabetic functional foods or nutraceuticals.
研究领域:
营养学
·
分子生物学
·
代谢研究
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DOI
PubMed
高影响因子
Analytical Methods
·
2021
·Vol. 13
·期 15
·1795-1802
·
IF 2.896
Feng Wang,De-Bin Wan,Yu-Dong Shen,Yuan-Xin Tian,Zhi-Li Xiao,Zhen-Lin Xu,Jin-Yi Yang,Yuan-Ming Sun,Bruce D. Hammock,Hong Wang
The natural mycotoxin tenuazonic acid (TeA) in foods is identified as the most toxic mycotoxin among the over 70 kinds of secondary toxic metabolites produced by Alternaria alternata. Some hapten–antibody-mediated immunoassays have been developed for TeA detection in food samples, but these methods show unsatisfactory sensitivity and specificity. In this study, a rationally designed hapten for TeA mycotoxin generated with computer-assisted modeling was prepared to produce a highly specific camel polyclonal antibody, and an indirect competitive chemiluminescence enzyme immunoassay (icCLEIA) was established with a limit of detection of 0.2 ng mL−1 under optimized conditions. The cross-reactivity results showed that several analogs and some common mycotoxins had negligible recognition by the anti-TeA polyclonal antibody. The average recoveries spiked in fruit juices were determined to be 92.7% with an acceptable coefficient of variation, and good correlations between icCLEIA and liquid chromatography tandem mass spectrometry (LC-MS/MS) results were obtained in spiked samples. This developed icCLEIA for TeA detection with significantly improved sensitivity and satisfactory specificity is a promising alternative for environmental monitoring and food safety.
研究领域:
食品科学
·
免疫学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
·
2015
·
IF 2.729
Zhui Tu,Yang Xu,Jinheng Fu,Zhibing Huang,Yao Wang,Bin Liu,Yong Tao
This work aimed to evaluate novel affinity resin used to purify immunoglobulin G (IgG) with a variable domain of the heavy chain of the heavy-chain antibody (VHH) as an affinity ligand. The VHH, isolated from a naïve camelid single-domain phage display library, exhibits not only affinity to the fragment crystallizable (Fc) region of IgG but also high thermal stability . This anti-Fc VHH (AFV) was expressed as a soluble protein in Escherichia coli and purified using a simple heat treatment procedure. The effects of pH and NaCl concentrations on the capacity of AFV resin were also investigated. Results showed a robust property of the AFV resin. It could bind IgGs at various pH conditions (from 6.0 to 9.0) and NaCl concentrations. The static binding capacities of AFV resin ranged from 3.40 ± 0.53 mg/ml to 15.04 ± 0.37 mg/ml measured using rabbit, mouse, and human IgGs. The bound IgGs can be efficiently eluted at pH 5.0, which is conducive to acid-sensitive IgGs and prevents the aggregation of IgGs. After 10 purification cycles or a 7-day period of storage at 37 °C, recovery did not decrease. These findings suggested that VHHs from non-immunized library could also be robust and functional reagent as an affinity purification ligand.
研究领域:
生物技术
·
生物化学
·
分子生物学
·
蛋白质工程
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产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
Jianmei Li,Qi Sun,Tuyatsetseg Jambal,Dulguun Dorjgotov,Na Su,Qiuju Wu,Jirimutu
To identify adulterated camel meat in emerging markets, there is an urgent need for sensitive, rapid, and convenient detection methods. In this study, a novel fluorescent sensing platform was developed to effectively identify pork components adulterated in camel meat by regulating the surface charge properties of MIL-101(Cr). Our data demonstrated that the modification of polyethylenimine (PEI) had minimal impact on the crystal structure of MIL-101(Cr). However, it significantly affected the adsorption behavior of MIL-101(Cr) towards 6-carboxyfluorescein (FAM) labelled DNA, thereby influencing the response of fluorescence quenching-recovery. Under optimal conditions, the proposed turn-on fluorescent biosensor, based on PEI-MIL-101(Cr) exhibited high sensitivity with a detection limit of 42 pM. It also demonstrated excellent selectivity for specific target DNA, successfully detecting a pork fraction of 0.45 % (w/w) in camel meat. This study was aimed to provide a fresh perspective on the potential application of functional materials derived from metal-organic frameworks in detecting food adulteration .
研究领域:
食品安全
·
生物传感
·
材料科学
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DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2023
·
IF 15.1
Qinghong Ji,Xinxin Sheng,Xiaolong Li,Shuang Liu,Qiufei Chen,Pengzong Guo,Yabi Yang,Youyou Huang,Guoliang Zhang,Xiang Lu,Jinping Qu
Infrared stealth materials are in increasing demand with the development of infrared detection technology. However, most reported materials focus solely on emissivity control or temperature control, resulting in brief and less ideal infrared stealth effect. Herein, a double layer MXene@polyvinyl alcohol (PVA)/phase change capsules (PCC) aerogel composite inspired by the skin-fat structure of camel for infrared camouflage was successfully fabricated via a facile freeze-drying followed by spraying method. The PVA/PCC aerogel composite with high latent heat (155.2 J/g) and strong mechanical properties (772.6 kPa), obtained by an in-situ one-step method, is adopted as a temperature control layer, benefiting from the prominent thermal insulation of porous structure and effective heat absorption of PCC. The low emissivity (0.249) of the MXene layer on the surface of aerogel composite makes its radiation temperature recognized by the thermal imager much lower than its actual temperature. By integrating thermal insulation, heat absorption and emissivity reduction strategies, MXene@PVA/PCC aerogel shows no difference with the background in the infrared thermal imager (radiation temperature difference less than 10 °C), achieving superior infrared stealth for medium and low temperature scenarios, and provides novel ideas for developing more high-performance infrared stealth materials.
研究领域:
材料科学
·
纳米技术
·
热工程
·
红外技术
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产品关联
DOI
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2025
·
IF 6.6
Jianmei Li,Yuting Yin,Haiyu Li,Zongyao Zhao,Mengqin Xiang,Yong Tang,Qi Sun
The escalating economic and ethical concerns associated with camel meat adulteration using cheaper horsemeat necessitate the development of rapid, on-site detection technologies that overcome the limitations of conventional laboratory-based methods. In this study, a portable electrochemical DNA biosensor was fabricated by integrating a polydopamine (PDA)-modified screen-printed carbon electrode (SPCE) with a nucleic acid-functionalized UIO-66(Zr) metal-organic framework (MOF). The PDA coating facilitated stable, covalent immobilization of DNA capture probes through Schiff base reactions, while the UIO-66(Zr) MOF functioned as a high-capacity signal amplifier owing to its large surface area and intrinsic redox activity. This synergistic architecture enabled ultrasensitive detection, achieving a limit of 3.57 pmol/L for horsemeat DNA and 0.5 g/kg adulteration in camel meat blends—significantly surpassing the performance of existing detection methods. The biosensor exhibited excellent specificity by distinguishing single-base mismatches, along with high reproducibility (RSD = 4.12%), operational stability over 15 days, and accurate recovery rates ranging from 91.7% to 112.4% in spiked samples. Its disposable electrode format, cost-effectiveness, and compatibility with portable potentiostats underscore its strong potential for on-site meat authentication. Overall, this work provides a practical and scalable solution to combat meat fraud in resource-limited settings and establishes a versatile MOF-electrode nanoassembly platform for advanced food safety monitoring.
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高影响因子
危险品化学品经营许可证（带存储）