Acta Biomaterialia
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2020
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IF 7.242
Junjun Tan,Yang Liu,Jing Gong,Xiaoying Jin,Cheng Cheng,Rong Zhang,Minfang Chen
Hydroxyapatite (HA) nanorods in the collagen matrix of bone have a macroscopically ordered structure that has many similarities to the ordered structure of anisotropic nano-units in inorganic liquid crystals (LCs). Inspired by these similarities, we conducted the first (to our best knowledge) synthesis of HA LCs in non-polar solvents (such as cyclohexane and toluene), thus expanding the range of applicable monomers and polymers. We synthesized HA nanorods by a simple, effective, and oleic-acid-assisted hydrothermal route. The hydrothermal temperature directly modulates the aspect ratio of the HA nanorods, and indirectly modulates their LC behavior. The LC phase transition has no size limitation. Thus, our approach may be used to develop high solid content, macroscopically assembled, large-scale polymer-based bio(mimetic)-materials.
研究领域:
材料科学
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生物医学工程
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DOI
PubMed
高影响因子
JOURNAL OF BIOACTIVE AND COMPATIBLE POLYMERS
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2021
·
IF 1.756
Xuqin Li,Zhou Lei,Jie Sheng,Yishan Song
In this study, a novel peptide grafted chitosan (CACS-FBP) with high peptide content, excellent moisture-absorption and moisture-retention abilities was prepared. Caffeic acid (CA) was used to modify chitosan, the highly water-soluble intermediate further reacted with fish bone collagen peptide to obtain the final product, and the synthesis of CACS-FBP was confirmed by the Fourier transform infrared spectroscopy (FT-IR), NMR, and UV-vis. The single-factor experiments indicated that the degree of substitution (DS) of CACS-FBP depended on the reaction temperature, reaction time, the mass ratio of fish bone collagen peptide to CACS (mFBP/mCACS) and the mass ratio of MTGase to CACS (mMTGase/mCACS). In addition, the antioxidant assay indicated that CACS-FBP had an excellent antioxidant capacity, and the CACS-FBP showed no cytotoxicity toward L929 mouse fibroblasts, all the results mean that the prepared peptide-containing chitosan derivative has potential application in pharmaceutical and biomedical fields.
研究领域:
生物化学
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药学
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材料科学
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DOI
高影响因子
Heliyon
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2023
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IF 4
Jie Wang,Huiwen Hou,Yan Li,Wen Tang,Didi Gao,Zengmei Liu,XinQing Gao,Feiyan Zhao,Feng Sun,Haining Tan,Juan Wang
Oxidative stress plays a crucial role in the development of osteoporosis. In this study, it was observed that donkey bone collagen (DC) at a concentration of 500 μg/mL scavenged 17.89 % of 1,1-Diphenyl-2-picrylhydrazyl (DPPH) free radicals, indicating its antioxidant properties. Additionally, when an oxidative damage osteoblast model was created using H 2 O 2 , 100 μg/mL DC demonstrated the ability to enhance cell survival by 27.31 %. Furthermore, 50 μg/mL DC increased the intracellular differentiation marker alkaline phosphatase (ALP) level by 62.65 %. Additionally, the study revealed that DC significantly increased the expression of osteoporosis-related factors in serum and effectively restored the abnormal structure of spongy bone in mice osteoporosis model. Peptides (GGWFL, ANLGPA, and GWFK) isolated from DC through gastrointestinal digestion and subsequent enzymatic purification in vitro demonstrated the ability to safeguard osteoblasts from H 2 O 2 -induced damage by reducing intracellular reactive oxygen species (ROS). This protection resulted in enhanced cell survival and promoted osteoblast differentiation. This investigation underscores that DC can shield oxidative damage osteoblast model from oxidative stress, ameliorate osteoporosis, and enhance bone density in mice osteoporosis model. These findings suggest various DC applications in the food and medicine industries.
研究领域:
生物化学
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医学
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营养学
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DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2024
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IF 7.7
Yanlan Ma,Xinyi Yang,Zongshuai Zhu,Tianran Huang,Jichao Huang,Ming Huang
In this study, chicken bone gelatin (CBG) was extracted as a new substitute for traditional pig bone gelatin, and bacterial cellulose (BC) was used as the compound to prepare Oregano essential oil (OEO) Pickering emulsion. To explore a protein/polysaccharide emulsion system that can effectively prolong the functional activity of OEO during storage. The results indicated that the variation in CBG and BC content significantly influenced the physicochemical properties of the emulsions. The optimal formulation of OEO Pickering emulsion, prepared with a CBG-BC ratio of 6:2 ( v /v), exhibited superior characteristics including appearance, encapsulation efficiency, and stability during preservation. After 7 d of storage at 4 °C, the rheological properties remained stable, with no significant differences observed in antioxidant and antibacterial activities. It was verified in the beef fresh-keeping experiment that the shelf life of beef samples in the 6-2-2 treatment group was 6 d longer than that in the control group and 3 d longer than that in the pure OEO group. This experiment enhanced the utilization of poultry by-products and provided a valuable reference for exploring suitable protein-polysaccharide systems embedding active substances for food preservation.
研究领域:
食品科学
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生物化学
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DOI
PubMed
高影响因子
Marine Drugs
·
2022
·Vol. 20
·期 1
·48
·
IF 6.085
Jian Li,Jun-Hui Cheng,Zhao-Jie Teng,Xia Zhang,Xiu-Lan Chen,Mei-Ling Sun,Jing-Ping Wang,Yu-Zhong Zhang,Jun-Mei Ding,Xin-Min Tian,Xi-Ying Zhang
Although the S8 family in the MEROPS database contains many peptidases, only a few S8 peptidases have been applied in the preparation of bioactive oligopeptides. Bovine bone collagen is a good source for preparing collagen oligopeptides, but has been so far rarely applied in collagen peptide preparation. Here, we characterized a novel S8 gelatinase, Aa2_1884, from marine bacteriumFlocculibacter collagenilyticusSM1988T, and evaluated its potential application in the preparation of collagen oligopeptides from bovine bone collagen. Aa2_1884 is a multimodular S8 peptidase with a distinct domain architecture from other reported peptidases. The recombinant Aa2_1884 over-expressed inEscherichia colishowed high activity toward gelatin and denatured collagens, but no activity toward natural collagens, indicating that Aa2_1884 is a gelatinase. To evaluate the potential of Aa2_1884 in the preparation of collagen oligopeptides from bovine bone collagen, three enzymatic hydrolysis parameters, hydrolysis temperature, hydrolysis time and enzyme-substrate ratio (E/S), were optimized by single factor experiments, and the optimal hydrolysis conditions were determined to be reaction at 60 ℃ for 3 h with an E/S of 400 U/g. Under these conditions, the hydrolysis efficiency of bovine bone collagen by Aa2_1884 reached 95.3%. The resultant hydrolysate contained 97.8% peptides, in which peptides with a molecular weight lower than 1000 Da and 500 Da accounted for 55.1% and 39.5%, respectively, indicating that the hydrolysate was rich in oligopeptides. These results indicate that Aa2_1884 likely has a promising potential application in the preparation of collagen oligopeptide-rich hydrolysate from bovine bone collagen, which may provide a feasible way for the high-value utilization of bovine bone collagen.Keywords:peptidase;the MEROPS S8 family;bovine bone collagen;oligopeptides;hydrolysate
研究领域:
生物化学
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酶学
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食品科学
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DOI
PubMed
高影响因子
Marine Drugs
·
2021
·Vol. 19
·期 12
·676
·
IF 5.118
Jun-Hui Cheng,Xiao-Yu Zhang,Zhen Wang,Xia Zhang,Shi-Cheng Liu,Xiao-Yan Song,Yu-Zhong Zhang,Jun-Mei Ding,Xiu-Lan Chen,Fei Xu
Bovine bone is rich in collagen and is a good material for collagen peptide preparation. Although thermolysin-like proteases (TLPs) have been applied in different fields, the potential of TLPs in preparing bioactive collagen peptides has rarely been evaluated. Here, we characterized a thermophilic TLP, A69, from a hydrothermal bacteriumAnoxybacillus caldiproteolyticus1A02591, and evaluated its potential in preparing bioactive collagen peptides. A69 showed the highest activity at 60 °C and pH 7.0. We optimized the conditions for bovine bone collagen hydrolysis and set up a process with high hydrolysis efficiency (99.4%) to prepare bovine bone collagen peptides, in which bovine bone collagen was hydrolyzed at 60 °C for 2 h with an enzyme–substrate ratio of 25 U/g. The hydrolysate contained 96.5% peptides that have a broad molecular weight distribution below 10000 Da. The hydrolysate showed good moisture-retention ability and a high hydroxyl radical (•OH) scavenging ratio of 73.2%, suggesting that the prepared collagen peptides have good antioxidative activity. Altogether, these results indicate that the thermophilic TLP A69 has promising potential in the preparation of bioactive collagen peptides, which may have potentials in cosmetics, food and pharmaceutical industries. This study lays a foundation for the high-valued utilization of bovine bone collagen.Keywords:thermolysin-like protease;bovine bone collagen;collagen peptides;collagen peptide preparation;bioactivity
研究领域:
生物化学
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食品科学
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药学
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化妆品
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产品关联
DOI
PubMed
高影响因子
Food Bioscience
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2024
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IF 4.8
Na Li,Zhangwen Liu,Wenhua Li,Linyan Yao,Jianxi Xiao
Osteoporosis is a prevalent systemic disease characterized by the change of bone structure and function, significantly affecting the human health. Oral collagen hydrolysates have garnered increasing attention for the prevention and treatment of osteoporosis due to their advantages of low molecular weight, easy absorption, and high biological activity; however, they suffer from severe concerns including uncontrollable molecular weights, limited biocompatibility, and unclear correlations between molecular weight and efficacy. Herein, we have for the first time developed a series of yak bone collagen hydrolysates (YBCH) with controllable molecular weight for effective prevention of osteoporosis. The YBCH exhibited low (LYBCH, M < 1000 Da), medium (MYBCH, 1000 < M < 3000 Da), high (HYBCH, 3000 < M < 10000 Da), and ultra-high (UYBCH, M > 10000 Da) molecular weight, respectively. An osteoporosis mouse model was established to evaluate the therapeutic effects of YBCH. Histological analysis indicated that YBCH could ameliorate pathological changes of osteoporosis by increasing bone mass and promoting collagen regeneration, with MYBCH showing the most pronounced effect. Micro-CT results revealed that MYBCH significantly enhanced the microstructure of osteoporotic bone by increasing BMD, BV/TV, Tb.N, and Tb.Th, while decreasing Tb.Sp and Po. These bioactive YBCH provide a novel strategy for the treatment of osteoporosis, presenting promising applications in nutritional supplements and functional food.
研究领域:
医学
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营养学
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产品关联
DOI
高影响因子
Cell Journal
·
2021
·
IF 2.479
Liping Zhou,Haiwei Ye,Lizhen Liu,Yunhua Chen
Objective Human bone marrow mesenchymal stem cell (hBMSC)-derived exosomes exhibit protective effects againstinflammatory diseases. This study aimed to explore the effects of hBMSC-derived exosomes on osteoarthritis (OA) invitro and its related mechanisms. Materials and Methods In this experimental study, we characterised exosomes derived from hBMSCs by transmissionelectron microscopy, nanoparticle tracking and Western blot analysis. Cellular uptake of exosomes was observed byfluorescent microscopy. Cell viability of chondrocytes exposed to interleukin-1 beta (IL-1β) was determined by theCell Counting Kit-8 (CCK-8). Real-time quantitative polymerase chain reaction (RT-qPCR) was used to determineexpression levels of genes related to apoptosis, inflammation, cartilage collagen metabolism and mitogen-activatedprotein kinases. Results Fluorescence microscopy revealed that hBMSC-derived exosomes could be taken up by chondrocytes.hBMSC-derived exosomes could significantly enhance cell viability of chondrocytes in response to IL-1β treatment.RT-qPCR showed significant up-regulation of Survivin, Versican, IL-1β, IL-6, NF-κB, MMP-13, MAPK p38, JNK, ERK,Aggrecan and SOX9 expression levels by IL-1β treatment, while their mRNA expression levels decreased after co-culture with exosomes. The anti-inflammatory gene TGF-β was markedly suppressed by IL-1β treatment; however, weobserved its expression after co-culture with exosomes. Additionally, the pro-inflammatory genes IL-1β, IL-6, NF-κB,TNF-α and TNF-β displayed significantly elevated expression levels in the IL-1β group and reduced expression levelsafter co-culture with exosomes. Conclusion hBMSC-derived exosomes may play a protective role in chondrocytes through inhibiting cell apoptosisand the inflammatory response. These results will provide a novel therapeutic strategy for OA.
研究领域:
干细胞生物学
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炎症
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骨科
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分子生物学
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DOI
PubMed
高影响因子
POLYMER TESTING
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2022
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IF 4.931
Chao Zhang,Bowei Du,Zihao Song,Guiya Deng,Yan Shi,Tianyu Li,Yaqin Huang
Antioxidant peptides from natural polymer collagen are highly desirable and their ability to coordinate with minerals will provide new leads for the prevention and treatment of oxidative stress-related diseases. Here, we tested the changes of structures and functional properties of bovine bone collagen peptides (BBCP) after magnesium chelation. The Fourier transform infrared (FTIR) results revealed that Mg(II) chelated with BBCP mainly via amino nitrogen and carboxyl oxygen atoms. The fluorescence and circular dichroism (CD) results indicated that Mg(II) could induce and stabilize the conformational structures, and change the amino acid distribution on the surfaces of BBCP. The chelation of BBCP and Mg(II) led to the improvement of radical scavenging activities, and enhanced the intracellular antioxidant enzyme activities and GSH levels, thus demonstrating higher protective and reparative effects against H 2 O 2 -induced oxidative damage. This work provides mechanistic insights for further development of peptide-magnesium chelates for oxidative stress mitigation.
研究领域:
生物化学
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药学
·
营养学
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）