CHEMICAL ENGINEERING JOURNAL
·
2024
·
IF 13.3
Rui Lei,Mingbao Gu,Jinyan Li,Wenjun Wang,Qian Yu,Rizwan Ali,Jiajia Pang,Meilun Zhai,Yang Wang,Kunxi Zhang,Jingbo Yin,Jinghong Xu
To avoid chronic wound formation caused by hypoxia and cold in high-altitude areas, developing an injectable, strongly adhesive hydrogel bandage with controllable oxygen generation and inflammation regulation functions is of great value. Poly(lipoic acid) (PLA)-based adhesives have been well-developed from polymerization of α -Lipoic acid (LA), showing attractive performances including simple preparation, strong adhesion, anti-inflammatory. However, injection of PLA-based adhesives has not been well-practiced because of its high viscosity and strong adhesion. Herein, LA and trometamol are firstly found to rapidly gel into a supramolecular hydrogel within 5 min at room temperature, exhibiting well-performed injectability. Further presence of Ce 3+ significantly accelerates this gelation within 2 min. Meanwhile, dopamine (DA) and g -C 3 N 4 nanosheet synergistically endow the injectable hydrogel with photothermal function, which can convert near-infrared-light (NIR) into heat, triggering LA polymerization and achieving post-injection enhancement. In terms of bandage performance after molding, the introduction of Ce 3+ , DA, and g -C 3 N 4 contributes to the improvement of mechanical and adhesion properties. The injectable hydrogel bandage not only possesses highly tough and adhesive features, but also produces oxygen under NIR irradiation, effectively alleviating wound hypoxia, creating anti-inflammatory microenvironment, and significantly promoting healing of incision in a low-pressure oxygen environment.
研究领域:
生物医学工程
·
材料科学
·
创伤修复
✓ 已验证
产品关联
DOI
高影响因子
Oxidative Medicine and Cellular Longevity
·
2022
·
IF 7.31
Xuemei Zou,Hailing Yang,Qiuyue Li,Ning Li,Ya Hou,Xiaobo Wang,Xianli Meng,Jia Yu,Yi Zhang,Ce Tang,Tingting Kuang
Brassica rapa L., a traditional Tibetan medicine, has been wildly used for treating plateau disease. Polysaccharide is an important chemical component in B. rapa. The present study aimed to evaluate the effect of B. rapa polysaccharide (BRP) against acute high-altitude hypoxia (AHH) induced brain injury and its metabolic mechanism. The rats were randomly divided into six groups: control group, AHH group, Hongjingtian oral liquid group, and three BRP groups (38, 75, and 150 mg/kg/d). Serum levels of superoxide dismutase (SOD), malondialdehyde (MDA), glutathione (GSH), oxidized glutathione (GSSG), and lactate dehydrogenase (LDH) were detected by commercial biochemical kits. Hippocampus and cortex histopathological changes were observed by H&E staining and Nissl staining. Neuronal apoptosis was observed by TUNEL staining. The protein and gene expression of Caspase-3, Bax, Bcl-2, p-PI3K, PI3K, p-Akt, Akt, HIF-1α, microRNA 210, ISCU1/2, and COX10 were detected by western blotting and qRT-PCR. Then, a brain metabolomics method based on UPLC-Q-Exactive-MS was performed to discover potential biomarkers and analyze metabolic pathways. It was found that BRP decreased levels of MDA, LDH, and GSSG, increased GSH and SOD, reduced the pathological changes, inhibited apoptosis, and activated the PI3K/Akt/HIF-1α signaling pathway as evidenced by increased phosphorylation of PI3K and Akt, enhanced protein expression of HIF-1α and gene levels of microRNA210, ISCU1/2, and COX10. Furthermore, 15 endogenous potential biomarkers were identified in the brain through metabolomics analysis. BRP can regulate 7 potential biomarkers and the corresponding metabolic pathways were mainly associated with pyruvate metabolism and glycolysis/gluconeogenesis. Collectively, BRP has a clear protective effect on AHH-induced brain injury and its mechanisms may be related to ameliorate oxidative stress injury, inhibit apoptosis by activating PI3K/Akt/HIF-1α signaling pathway, and reverse metabolic pathway disturbances.
研究领域:
药理学
·
神经科学
·
代谢组学
·
生物化学
·
分子生物学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）