PLoS One
·
2016
·Vol. 11
·期 3
·e0149257
·
IF 3.057
Chao Fang,Weihong Lu,Chengyan Li,Xi Peng,Yang Wang,Xiulan Huang,Zhihong Yao,Nali Cai,Yuge Huang,Xingliang Zhang,Jianxin Tan
Asthma is a common chronic respiratory disease. In a previous study, we found several circulating microRNA signatures associated with childhood asthma and selected miR-3162-3p for subsequent studies. Since the target proteins and underlying molecular mechanisms of miR-3162-3p in asthma etiopathogenesis are not well characterized, we designed this study to clarify its role. We employed bioinformatics and quantitative PCR methods as a first step to determine the target of miR-3162-3p, and we elucidated β-catenin. Luciferase assays and western blot analysis confirmed β-catenin as a direct target of miR-3162-3p as the 3’-untranslated region of β-catenin mRNA possesses a specific miR-3162-3p pairing site. The correlation between the expression levels of miR-3162-3p and β-catenin is confirmed by quantitative PCR and western blot studies in A549, Beas-2B and H1299 cell lines and OVA-induced asthma mouse model. Of note, upregulation of the endogenous miR-3162-3p level is concomitant with the reduction of β-catenin mRNA and protein expression levels. MiR-3162-3p antagomir treatment antagonizes the endogenous miR-3162-3p and effectively rescues the attenuation of endogenous β-catenin in OVA-induced asthmatic mice, which alleviates airway hyperresponsiveness and ameliorates airway inflammation. Collectively, our findings suggest a novel relationship between miR-3162-3p and β-catenin and clarify their mechanistic role in asthma etiopathogenesis.
研究领域:
分子生物学
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呼吸病学
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免疫学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF LUMINESCENCE
·
2021
·
IF 3.599
Ruxia Zhang,Zhefeng Fan
A novel redox-controlled fluorescent sensor for the detection of highly reactive oxygen species (hROS) was developed based on the unique green fluorescence emission of dihydrolipoic acid functionalized carbon quantum dots (DHLA@N-CQDs). Given the excellent redox characteristic between the disulfide bonds (S–S) and thiol (R-SH), the sensor was designed by bonding N-CQDs and DHLA under the action of coupling agents. When hROS was introduced into the sensing system, a large number of S–S gathered between DHLA@N-CQDs can effectively inhibit the fluorescence of probe. In addition, the as-prepared fluorescent sensor can sense hydroxyl radicals (·OH) and hydrogen peroxide (H 2 O 2 ) in different response ranges, which ensures a high selectivity for hROS detection. Furthermore, the sensor system has been successfully utilized to detect the H 2 O 2 in human saliva samples and the fluorescence imaging of ·OH in HeLa cells, exhibiting the feasibility of fluorescent sensors in practical applications.
研究领域:
生物化学
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医学检测
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纳米材料科学
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产品关联
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2016
·
IF 5.31
Yali Xu,Junjie Hao,Xiaoying Niu,Shengda Qi,Hongli Chen,Kai Wang,Xingguo Chen,Tao Yi
Here we demonstrate the seed-mediated growth approach, one of the most reliable and versatile methods to control the size and shape of nanocrystals (NCs), for rapid synthesis of high performance red-emitting core/shell CdTe quantum dots (QDs) in aqueous phase. Critical parameters (including the pH value, Cd:Te molar ratio, MPA:Cd molar ratio, and the amount of CdTe seeds) affecting the growth of core/shell CdTe QDs are systematically introduced and analyzed. Under optimal conditions, the growth behaviors can be well controlled, and the high performance typically offered by the seed-mediated growth method was obtained as expected. The emission peak position quickly shifted from 540 to 680 nm in 30 min, and the red-emitting CdTe QDs thus obtained performed well, giving good size distribution, narrow full width at half maximum and high quantum yield. The growth mechanism and the resulting high structural and optical properties were also elucidated. Finally, the obtained CdTe QDs were successfully applied to highly reactive oxygen species (hROS) sensing, suggesting that this method may be useful in bioimaging and biolabeling.
研究领域:
纳米材料
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生物医学工程
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分析化学
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DOI
高影响因子
JOURNAL OF POLYMER SCIENCE PART A-POLYMER CHEMISTRY
·
2013
·Vol. 51
·期 8
·1690-1694
·
IF 3.543
Yun-Hai Yu,Xiao-Hui Liu,Di Jia,Bo-Wen Cheng,Yuan-Lin Ren,Fei-Jun Zhang,Hui-Na Li,Peng Chen,Shan Xie
Graphical A universal high reactive ligand, Me 6 TREN, was successfully explored as the reducing agent for the ambient temperature copper-mediated LRP of AN. The key to success is ascribed to the optimized Me 6 TREN amount and reaction conditions employed, producing ideal living free radical polymerization characteristics. The very high chain-end functionality of the resultant polymer has been confirmed by GPC and 1 H NMR analyses as well as the chain-extension reaction.
研究领域:
高分子化学
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聚合反应
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功能高分子
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可控自由基聚合
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材料化学
✓ 已验证
DOI
高影响因子
FUEL
·
2022
·
IF 8.035
Yanli Zhu,Wei Le,Wanjun Zhao,Xiaohang Ma,Dazhi Liu,Jianxin Li,Qingjie Jiao
To improve the heat release and reactivity of Al, Li was incorporated into Al via the centrifugal atomization in this study for obtaining Al-Li alloy powders. The microstructure and composition of the samples were characterized by SEM, XRD, and ICP. The oxidation kinetics were evaluated via TG/DSC, and the energetic performance of the Al-Li/KP composites was tested. The results show that spherical Al-Li alloy powders with the well-distributed dendritic structure are obtained that are mainly composed of αAl and Al-Li compounds. The oxidization rate of Al-Li alloy powders is higher than pure Al, owing to the decreasing activation energy caused by the catalysis of the soluble Li in Al and Al-Li compounds. The shorter ignition delay and higher reaction rate of Al-Li/KP with increasing Li content in Al-Li alloy powders is a result of the microexplosion of Al-Li. Therefore, Al-Li alloy powders with high reactivity are promising fuels for energetic materials.
研究领域:
材料科学
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能量材料
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化学工程
✓ 已验证
DOI
高影响因子
Biomass Conversion and Biorefinery
·
2024
·
IF 3.5
Gong Xiujin,Wang Dengfeng,Zheng Qiannan,Liu Lin,Wu Han,Li Zihan,Hong Haojie,Yao Juming
Lignin extraction and upgrade are the great importance to lignin valorization but are closely related to raw material sources, separation methods, and conditions. The advantage of this work is to firstly exploit an efficient and practical approach to extract highly reactive lignin from waste mulberry branches (MB) using green deep eutectic solvent (DES). To better understand the relationship between the extraction conditions and the properties of lignin, the influences of DES components, extraction temperature and time on the extraction efficiency, average molecular weight, chemical structure, and thermal stability of lignin were systematically investigated and analyzed. The synergistic effects of processing conditions resulted in high extraction efficiency with 62.9% of yield and 94.5% of purity. Under optimal conditions with LA/ChCl ratio of 2:1, extraction temperature of 150 °C, and time of 6 h, the obtained lignin had relatively low molecular weight ( M n = 1900 g/mol and M w = 2841 g/mol) and narrow distribution (PDI = 1.49), simultaneously had high total hydroxyl content of 9.15 mmol/g. Benefiting from high hydroxyl content and low molecular weight, the extracted lignin possessed excellent miscibility and reactivity which could participate in polymerization reaction to synthesize lignin-derived rigid polyurethane foam (LRPUF). The integration of the highly reactive lignin significantly improved the specific compressive strength and modulus of LRPUF to 6.4 and 76.0 MPa/(g/cm 3 ), which increased by 7% and 22.4% than those of petroleum-based RPUF. Therefore, this work not only provided an efficient and practical strategy for lignin extraction and upgrade, but also proposed a potential of high value-added utilization of waste mulberry branches. Graphical Abstract
研究领域:
化学工程
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材料科学
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环境科学
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生物资源技术
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产品关联
DOI
高影响因子
JOURNAL OF CONTROLLED RELEASE
·
2024
·
IF 10.5
Xu Zuo,Xiaoping Guo,Dan Zhao,Yinuo Gu,Zheng Zou,Yuanyuan Shen,Chaoliang He,Caina Xu,Yan Rong,Fang Wang
Asthma is a chronic and heterogeneous disease affecting the lungs and respiratory tract. In particular, the neutrophil subtype of asthma was described as persistent, more severe, and corticosteroid-resistant. Growing evidence suggested that nontypeable Haemophilus influenzae (NTHi) infection contributes to the development of neutrophilic asthma, exacerbating clinical symptoms and increasing the associated medical burden. In this work, arginine-grafted chitosan (CS-Arg) was ionically cross-linked with tris(2-carboxyethyl) phosphine (TCEP), and a highly-efficient antimicrobial agent, poly-ε-L-Lysine (ε-PLL), was incorporated to prepare ε-PLL/CS-Arg/TCEP (ECAT) composite nanogels. The results showed that ECAT nanogels exhibited highly effective inhibition against the proliferation of NTHi, Staphylococcus aureus ( S. aureus ) and Escherichia coli ( E. coli ). In addition, ECAT nanogels could effectively inhibit the formation of mucins aggregates in vitro , suggesting that the nanogel might have the potential to destroy mucin in respiratory disease. Furthermore, in the ovalbumin (OVA)/NTHi-induced Balb/c mice model of neutrophilic asthma, the number of neutrophils in the alveolar lavage fluid and the percentage of inflammatory cells in the blood were effectively reduced by exposure to tower nebulized administration of ECAT nanogels, and reversing airway hyperresponsiveness (AHR) and reducing inflammation in neutrophilic asthma mice. In conclusion, the construction of ECAT nanogels was a feasible anti-infective and anti-inflammatory therapeutic strategy, which demonstrated strong potential in the clinical treatment of neutrophilic asthma.
研究领域:
药理学
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呼吸医学
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纳米技术
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免疫学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Cell Metabolism
·
2024
·
IF 27.7
Kan Huang,Zilun Li,Xi He,Jun Dai,Bingding Huang,Yongxia Shi,Dongxiao Fan,Zefeng Zhang,Yunchong Liu,Na Li,Zhongyu Zhang,Jiangyun Peng,Chenshu Liu,Renli Zeng,Zhipeng Cen,Tengyao Wang,Wenchao Yang,Meifeng Cen,Jingyu Li,Shuai Yuan,Lu Zhang,Dandan Hu,Shuxiang Huang,Pin Chen,Peilong Lai,Liyan Lin,Jielu Wen,Zhengde Zhao,Xiuyi Huang,Lining Yuan,Lifang Zhou,Haoliang Wu,Lihua Huang,Kai Feng,Jian Wang,Baolin Liao,Weiping Cai,Xilong Deng,Yueping Li,Jianping Li,Zhongwei Hu,Li Yang,Jiaojiao Li,Youguang Zhuo,Fuchun Zhang,Lin Lin,Yifeng Luo,Wei Zhang,Qianlin Ni,Xiqiang Hong,Guangqi Chang,Yang Zhang,Dongxian Guan,Weikang Cai,Yutong Lu,Fang Li,Li Yan,Meng Ren,Linghua Li,Sifan Chen
Thrombosis represents the leading cause of death and disability upon major adverse cardiovascular events (MACEs). Numerous pathological conditions such as COVID-19 and metabolic disorders can lead to a heightened thrombotic risk; however, the underlying mechanisms remain poorly understood. Our study illustrates that 2-methylbutyrylcarnitine (2MBC), a branched-chain acylcarnitine, is accumulated in patients with COVID-19 and in patients with MACEs. 2MBC enhances platelet hyperreactivity and thrombus formation in mice. Mechanistically, 2MBC binds to integrin α2β1 in platelets, potentiating cytosolic phospholipase A2 (cPLA2) activation and platelet hyperresponsiveness. Genetic depletion or pharmacological inhibition of integrin α2β1 largely reverses the pro-thrombotic effects of 2MBC. Notably, 2MBC can be generated in a gut-microbiota-dependent manner, whereas the accumulation of plasma 2MBC and its thrombosis-aggravating effect are largely ameliorated following antibiotic-induced microbial depletion. Our study implicates 2MBC as a metabolite that links gut microbiota dysbiosis to elevated thrombotic risk, providing mechanistic insight and a potential therapeutic strategy for thrombosis.
研究领域:
心血管研究
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微生物组学研究
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血栓机制
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代谢组学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF ETHNOPHARMACOLOGY
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2022
·
IF 5.195
Liping Zhou,Mengyang Hao,Xinsheng Fan,Zishan Lao,Mengwen Li,Erxin Shang
Ethnopharmacological relevance Houpo Mahuang Decoction (HPMHD is one of the classic traditional Chinese prescriptions that has been used in the treatment of asthma. The therapeutic effects and mechanism of HPMHD in aggravated asthma remain to be explored, especially from the perspective of metabolomics and Transient Receptor Potential Vanilloid-1 (TRPV1)/Ca 2+ /Tight junction (TJ) regulation. Aim of the study To investigate the therapeutic and metabolic regulatory effects and the underlying mechanism of HPMHD in asthmatic rats. Materials and methods The asthmatic rats were administered with the corresponding HPMHD (at dosages of 5.54, 11.07, 22.14 mg/kg). Then inflammatory cells in peripheral blood and bronchoalveolar lavage fluid (BALF) were counted, the levels of interleukin (IL)-4 and IL-13 in BALF were measured, and the changes in enhanced pause (Penh) and pathological damage of lung tissues were also detected to evaluate the protective effects of HPMHD. The serum metabolic profile of HPMHD in asthmatic rats was explored using Ultra-High-Performance Liquid Chromatography-mass spectrometer (UHPLC-MS), and the regulatory effects on TRPV1 and TJs of HPMHD in asthmatic rats were detected by Western blotting analysis. In vitro , 16HBE cells were stimulated with IL-4 plus SO 2 derivatives and then administered HPMHD. The intracellular Ca 2+ regulated by TRPV1, and the expression levels of TRPV1 and TJ proteins (TJs) were then detected by calcium imaging and Western blotting. The effects were verified by inhibition of TRPV1 and in short hairpin RNA (shRNA)-mediated TRPV1 silencing cells. Results HPMHD significantly attenuated the airway inflammation of asthmatic rats, and reduced the levels of inflammatory cells in peripheral blood and BALF as well as the levels of IL-4 plus IL-13 in BALF. In addition, the airway hyperresponsiveness and lung pathological damage were alleviated. Serum metabolomic analysis showed that 31 metabolites were differentially expressed among the normal saline-, model-, and HPMHD-treated rats. Pathway enrichment analysis showed that the metabolites were involved in 45 pathways, among which, TJs regulation-relevant pathway was associated with the Ca 2+ concentration change mediated by the TRP Vanilloid channel. In vivo and in vitro experiments indicated that HPMHD reduced the concentration of intracellular Ca 2+ via suppressing the expression and activation of TRPV1, increased the expression of ZO-1, Occludin , and Claudin-3, and protected the integrity of TJs. Conclusion The current study indicates that HPMHD alleviates rat asthma and participates in the regulation of serum metabolism. The anti-asthma effects of HPMHD might be related to the protection of TJs by inhibiting the intracellular Ca 2+ concentration via TRPV1.
研究领域:
药理学
·
免疫学
·
代谢组学
·
肺科学
·
中医
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ADVANCED FUNCTIONAL MATERIALS
·
2025
·
IF 19
Jian Wang,Xinxing Zeng,Fude Nie,Renming Pan,Jie Chen,Yaofeng Mao,Haifu Wang,Xingquan Zhang,Jun Wang
Transient microchips have attracted considerable attention in the field of information security and privacy protection due to their self-destruction capability. Metastable intermolecular composites (MICs) are appealing for such applications due to their high energy density and rapid reaction kinetics, which meet the demands for miniaturization and fast response in transient microchips. However, weak interfacial interactions in conventional MICs limit the contact between components, hindering both microchip integration and ignition reliability. Herein, a hydrogen-bond assembly strategy is proposed to fabricate BC/GO/Al/CuO energetic films with exceptional flexibility and superior combustion performance. Dopamine modification introduces abundant hydroxyl groups onto the MIC particle surfaces, enabling the formation of a continuous hydrogen-bond network with binder bacterial cellulose (BC) binder and functional additive graphene oxide (GO).The integrated transient microchips retain structural integrity after 25 drop tests from 50 cm height, and the BC/GO/Al/CuO films are reliably ignited using a 3 V/3 A power supply, inducing complete destruction to the silicon substrate. This work demonstrates a novel strategy for creating highly flexible and reactive energetic films, showing great promise for application in advanced transient microchips.
研究领域:
材料科学
·
微电子学
·
能源材料
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柔性电子学
·
信息安全
✓ 已验证
产品关联
DOI
高影响因子
Frontiers in Cardiovascular Medicine
·
2024
·
IF 2.8
Chen Hui-Lian,Wang Qing-Yu,Qi Ruo-Mei,Cai Jian-Ping
Background: Platelet hyperreactivity is a risk factor for thrombosis in elderly patients with cardiovascular diseases. However, the mechanism of platelet hyperactivation has not been elucidated. This study aims to investigate alterations in the proteomes of platelets and their correlation with platelet hyperreactivity among elderly individuals.Methods: This study included 10 young (28.1 ± 1.9 years), 10 middle-aged (60.4 ± 2.2 years), and 10 old (74.2 ± 3.0 years) subjects. Washed platelets were used in the present study. Platelet samples were analysed by using data-independent acquisition (DIA) quantitative mass spectrometry (MS).Results: The results showed that the platelet proteomic profile exhibited high similarity between the young and middle-aged groups. However, there were significant differences in protein expression profiles between the old group and the young group. By exploring the dynamic changes in the platelet proteome with ageing, clusters of proteins that changed significantly with ageing were selected for further investigation. These clusters were related to the initial triggering of complement, phagosome and haemostasis based on enrichment analysis. We found that platelet degranulation was the major characteristic of the differentially expressed proteins between the old and young populations. Moreover, complement activation, the calcium signalling pathway and the nuclear factor-κB (NF-κB) signalling pathway were enriched in differentially expressed proteins.Conclusions: The present study showed that there are obvious differences in the protein profiles of the elderly compared with young and middle-aged populations. The results provide novel evidence showing changes in platelet hyperactivity and susceptibility to thrombosis in the elderly population.
研究领域:
血液学
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蛋白质组学
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衰老研究
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心血管疾病
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Applied Bio Materials
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2022
·
IF 0
Shan Huang,Xinhao Han,Ying Liu,Pengcheng Huang,Ke-Yu Deng,Fang-Ying Wu
Detection and imaging of highly reactive oxygen species (hROS) in biological systems using fluorescent probes are critical for the study of physiological and pathological processes induced by hROS. Herein, we report a redox-active luminescent metal–organic framework (MOF), which incorporates a hydroquinone moiety that can undergo a reversible transformation from the hydroquinone to the quinone by hROS like •OH and ClO–. Moreover, the intrinsic fluorescence originating from the excited-state intramolecular proton transfer (ESIPT) property of the organic linker can be finely regulated during this redox-switchable process. A reversible fluorescent probe for hROS is thus developed. The presented probe shows a sensitive, selective, and reversible response to hROS due to the integration of excellent structural characteristics and unique spectral properties of the MOF. The detection limits of •OH and ClO– are 0.22 and 0.18 μM, respectively. Furthermore, with good photostability and super biocompatibility, this simple yet efficient fluorescent probe has been successfully applied to dynamic monitoring of endogenous and exogenous •OH and ClO– in live cells.
研究领域:
分析化学
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生物化学
·
材料科学
·
细胞生物学
✓ 已验证
产品关联
DOI
PubMed
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