JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
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2019
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IF 2.813
Shang-Zhen Li,Xiang-Ming Guan,Zhao Gao,Hong-Ci Lan,Qiang Yin,Chu Chu,De-Po Yang,E-Hu Liu,Ping Zhou
Citri Reticulatae Pericarpium (CRP), the dried pericarp of Citrus reticulata Blanco, can be divided into “Guangchenpi” (GCP, the dried pericarps derived from Citrus reticulata ‘Chachi’) and “Chenpi” (CP, the dried pericarps derived from other cultivars of Citrus reticulata Blanco). To discriminate between GCP and CP, a simple and reliable high-performance thin-layer chromatography (HPTLC) method was firstly developed to analyze the volatile compound dimethyl anthranilate , and a high-performance liquid chromatography (HPLC) method was established to simultaneously quantify dimethyl anthranilate and three predominant flavonoids (hesperidin, nobiletin and tangeretin) in CRP samples. Both the HPTLC analysis and HPLC-orthogonal partial least squares discrimination analysis (OPLS-DA) indicated that GCP can be effectively distinguished from CP based on analysis of dimethyl anthranilate. Our results indicated that dimethyl anthranilate can be used as a marker compound for discrimination of GCP and CP. This work provided a convenient approach which might be applied for quality evaluation of CRP.
研究领域:
中药学
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分析化学
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DOI
PubMed
高影响因子
International Journal of Environmental Research and Public Health
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2022
·Vol. 19
·期 24
·16879
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IF 4.614
Tong Li,Zhibo Wang,Yue Shi,Xiaolong Yao
Based on the porous carbon material from citric acid residue, catalysts of different Ce-Mn ratios were prepared with incipient-wetness impregnation (IWI) to delve into their acetone-degrading performance and relevant mechanisms. When the Ce-Mn molar ratio is 0.8, the prepared catalyst Ce0.8-Mn/AC shows abundant and uniformly dispersed Mn and Ce particles on the surface. The content of Mn and Ce on the Ce0.8-Mn/AC surface reaches 5.64% and 0.75%, respectively. At the acetone concentration of 238 mg/m3(100 ppm), the laws of acetone degradation in different catalysts at different catalyzing temperatures and with various oxygen concentrations were studied, and we found that the rate of acetone degradation by Ce0.8-Mn/AC can exceed 90% at 250 °C. Cerium oxide and manganese oxide are synergistic in the catalytic degradation of acetone. Adding cerium to manganese-based catalysts can increase the oxygen migration rate in the catalysts and thus raise the reduction rate of lattice oxygen in manganese oxide. The results offer new ideas and approaches for the efficient and comprehensive utilization of bio-fermentation by-products, and for the development of cheap and high degradation performance catalysts for acetone.Keywords:acetone;Ce0.8-Mn/AC;efficient degradation;low temperatures
研究领域:
环境科学
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催化材料
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DOI
PubMed
高影响因子
TRANSACTIONS OF NONFERROUS METALS SOCIETY OF CHINA
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2022
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IF 3.752
Hong-fu LIN,Wei WENG,Shui-ping ZHONG,Guan-zhou QIU
High-efficiency recovery of Zn and Pb from silicon-rich zinc leaching residues is realized in a rotary kiln. Characterizations by means of XRD, SEM, EDS and ICP reveal that the leaching residue contains 12.4 wt.% SiO 2 , 16.1 wt.% Zn, and 7.4 wt.% Pb. Thermodynamic analysis shows that metallic vapor of Zn and Pb can be easily generated from the zinc leaching residue at 1150−1250 °C inside the rotary kiln. Viscosities and melting points of 13 slag compositions were analyzed and three slag compositions (47wt.%SiO 2 −23wt.%CaO−30wt.%FeO, 40wt.%SiO 2 − 28wt.%CaO−32wt.%FeO, and 40wt.%SiO 2 −30wt.%CaO−30wt.%FeO) possessed the desirable physical properties, with the melting point and viscosity in the range of 1150−1280 °C and 0.2−0.5 Pa·s, respectively. The industrial tests show that adopting the optimized slag composition can contribute to very high recovery rates of Zn and Pb (97.3% for Zn and 94.5% for Pb), corresponding to slags with very low average contents of Zn and Pb (0.51 wt.% Zn and 0.45 wt.% Pb). The National-Standard leaching tests of the water-quenched slags result in 1.82 mg/L Zn, ~0.01 mg/L Cu, 0.0004 mg/L As, ~0.01 mg/L Cd, 0.08 mg/L Pb, and ~0.02 mg/L Hg in the leachate, verifying the detoxification of the zinc leaching residue at the same time.
研究领域:
冶金工程
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环境科学
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DOI
高影响因子
Fire-Switzerland
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2023
·Vol. 6
·期 1
·14
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IF 3.2
Jiaxiang Gao,Hanguang Wu,Yang Xiao,Wenjing Ma,Fei Xu,Rui Wang,Zhiguo Zhu
It remains a challenge to prepare flame-retardant composites via the addition of a low content of flame retardant. In this work, a novel DOPO-functionalized reduced graphene oxide hybrid (DOPO-M-rGO) flame-retardant system was synthesized for epoxy resin (EP). The phosphorus-nitrogen-reduced graphene oxide ternary synergistic effect provided DOPO-M-rGO with high flame-resistance efficiency in EP; thus, the EP-based composite exhibited superior fire-resistant performance at extremely low DOPO-M-rGO loading. The limiting oxygen index (LOI) of the EP-based composite was increased from 25% to 32% with only 1.5 wt% DOPO-M-rGO addition, and the peak heat release rate (pHRR), total heat release (THR), and total smoke production (TSP) were significantly decreased by 55%, 30%, and 20%, respectively. In addition, as a halogen-free flame-retardant system, DOPO-M-rGO presents great application potential as an eco-friendly additive for the flame-resistance improvement of thermosetting polymer materials.Keywords:flame retardant;epoxy resin;synergistic effect;high efficiency
研究领域:
材料科学
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化学工程
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DOI
高影响因子
Biology-Basel
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2020
·Vol. 9
·期 7
·171
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IF 3.796
Jianming Zhang,Yushan Bu,Chengcheng Zhang,Huaxi Yi,Daqun Liu,Jingkai Jiao
At present, De Man, Rogosa and Sharpe (MRS) broth is the medium of choice for promoting bacteriocin production. However, this medium is expensive and not applicable for large-scale production. Therefore, a low-cost and high-efficiency culture medium for bacteriocin Lac-B23 production byLactobacillus plantarumJ23 was developed. First, the effects of the composition of MRS broth on bacteriocin Lac-B23 production and bacterial growth were researched by a one variable at a time approach. Then, a Plackett-Burman design was used to screen significant components for production. Finally, the steepest ascent and central composite designs were used to obtain an optimum medium. The final composition of the modified MRS was much simpler than MRS broth, and the modified MRS contained only glucose, yeast extract, dipotassium phosphate, manganese sulfate monohydrate, Tween 80 and sodium acetate anhydrous. The highest bacteriocin Lac-B23 production reached 2560 activity units (AU)/mL in the modified MRS, which is nine times higher than that in MRS broth (280 AU/mL). Meanwhile, the cost per liter of the modified MRS (8.56 Ren Min Bi (RMB)/L) is 34.70% the cost of MRS broth (13.11 RMB/L), and the cost per arbitrary units of bacteriocin Lac-B23 in the modified MRS is approximately fourteen times more convenient (3.34 RMB/106AU) than in the MRS broth (46.82 RMB/106AU).Keywords:bacteriocin Lac-B23;low-cost;high-efficiency;culture medium
研究领域:
微生物学
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食品科学
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DOI
PubMed
高影响因子
Membranes
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2021
·Vol. 12
·期 1
·57
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IF 4.106
Huan Liu,Quan Li,Benqiao He,Zhengguang Sun,Feng Yan,Zhenyu Cui,Jianxin Li
The efficient separation of Sr2+/Mg2+through nanofiltration (NF) technology is a great challenge because Sr2+and Mg2+ions are congeners with the same valence and chemical properties. In this work, an NF membrane under an electric field (EF) was successfully employed to separate Mg2+and Sr2+ions for the first time. The effects of current densities, Mg2+/Sr2+mass ratios, pH of the feed, and coexisting cations on separation performance were investigated. Dehydration of Sr2+or Mg2+ions under EF was proved by molecular dynamics simulation. The results showed that a high-efficient separation of Mg2+/Sr2+was achieved: Mg2+removal of above 99% and increase in Sr2+permeation with increasing EF. A separation factor reached 928 under optimal conditions, far higher than that without EF. The efficient separation of Mg2+/Sr2+ions was mainly due to rejection of most Mg2+ions by NF membrane and in situ precipitation of partly permeated Mg2+ions by OH−generated on the cathode under EF. Meanwhile, preferential dehydration of Sr2+ions under EF due to lower hydration energy of Sr2+compared with Mg2+resulted in an increase of permeation of Sr2+ions. This work provided a new idea for separation of congener ions with similar valence and chemical properties.Keywords:ENF process;congener ions;dehydration;efficient separation
研究领域:
环境科学
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化学工程
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DOI
PubMed
高影响因子
Polymers
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2023
·Vol. 15
·期 10
·2359
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IF 5
Xiaohua Gu,Xiaoyao Wang,Xinyu Guo,Siwen Liu,Chunhua Lou,Yan Liu
In this paper, the high-efficiency degradation and alcoholysis recovery of waste polyurethane foam were realized using a combination of a high-efficiency alkali metal catalyst (CsOH) and two-component mixed alcoholysis agents (glycerol and butanediol) in different proportions, using recycled polyether polyol and one-step foaming to prepare regenerated thermosetting polyurethane hard foam. The foaming agent and catalyst were adjusted experimentally to prepare regenerated polyurethane foam, and a series of tests were conducted on the viscosity, GPC, hydroxyl value, infrared spectrum, foaming time, apparent density, compressive strength, and other properties of the degradation products of the regenerated thermosetting polyurethane rigid foam. The resulting data were analyzed, and the following conclusions were drawn: The optimal conditions of alcoholysis were obtained when the mass ratio of glycerol to butanediol was 3:2, the amount of cesium hydroxide was 0.08%, the reaction temperature was 170 °C, and the reaction time was 2.5 h. Regenerated polyurethane foam with an apparent density of 34.1 kg/m3and a compressive strength of 0.301 MPa was prepared under these conditions. It had good thermal stability, complete sample pores, and a strong skeleton. At this time, these are the best reaction conditions for the alcoholysis of waste polyurethane foam, and the regenerated polyurethane foam meets various national standards.Keywords:polyurethane foam;high-efficiency catalyst;recycling;glycolysis
研究领域:
材料科学
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化学工程
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产品关联
DOI
PubMed
高影响因子
Bioanalysis
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2025
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IF 1.8
Yonghua Yu,Danrui Liu,Hangyu Zhao,Menglong Dai,Yu Hu,Kaiwei Luo,Huina Zhang
Background Current HPLC-based methods for doxofylline analysis lack speed and precision. A rapid, specific, and sensitive UPLC-MS/MS method was developed for the determination of doxofylline in this study.Research design and methods This method was fully validated and doxophylline-d4 was used as an internal standard. A Kinetex-C18 column (EVO 100Å, 50 × 2.1 mm, 5 μm) was used for the separation procedure, with mobile phases consisting of 0.3% formic acid (A) and 90% acetonitrile solution with 0.3% formic acid (B). The total runtime of the gradient elution procedure was 2.6 minutes. The mass spectrometry analysis was carried out employing a multiple reaction monitoring model and using the transitions of m/z 267.000→181.000 for doxofylline and m/z 271.200→181.100 for the internal standard.Results The linear range of detection for doxophylline was between 20.0 to 16,000 ng/mL. The intra-batch accuracy deviations of each concentration level ranged from −8.0% to 2.5%, while the intra-batch precisions ranged from 1.3% to 9.0%. And the inter-batch accuracy deviations were −5.8% ~0.8%, while the inter-batch precisions were 2.2% ~7.0%.Conclusions This method was applied to pharmacokinetic clinical trials of single oral administration of doxophylline tablets successfully.Clinical trial registration www.clinicaltrials.gov identifier is CTR20240006 and CTR20233665
研究领域:
分析化学
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药代动力学
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药物研发
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临床研究
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产品关联
DOI
PubMed
高影响因子
MOLECULES
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2019
·Vol. 24
·期 11
·2150
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IF 3.06
Yingying Han,Jinhua Du,Jie Li,Miaomiao Li
Hawthorn wine is rich in anthocyanins, polyphenols, flavonoids and other macromolecular substances, which results in difficulty to rapidly determine organic acids in the wine. An enzymatic method is accurate but expensive and not able to quantify all of the organic acids simultaneously. Therefore, in this study, two HPLC methods were applied to quantify the organic acids in the wine with the enzymatic method as a reference. Seven organic acids were found with the enzymatic method including citric, succinic,l-malic, acetic, lactic, pyruvic, and fumaric acids, in which citric and succinic acid accounted for more than 80% of the total acids. By an 87H column equipped with DAD (diode array) detector at 215 nm (HPLC method 1), only citric and lactic acids were quantified accurately and the elution period was shortened from 100 min to 20 min by removing the impurity in the sample with a LC-18 SPE(solid-phase extraction) tube. While citric, succinic,l-malic, acetic, pyruvic, and fumaric acids were quantified reliably by a dC18 column equipped with DAD detector at 210 nm (HPLC method 2), with the sample requires only dilution and filtration before injection. It was suggested that HPLC method 2 was an effective method to quantify the organic acids in hawthorn wine. The method provides a choice for accurate quantification of organic acids in hawthorn wine or other drinks, and would be helpful for controlling the quality of hawthorn wine.Keywords:hawthorn wine;organic acids;high-performance liquid chromatography methods;enzymatic method
研究领域:
食品化学
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分析化学
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产品关联
DOI
PubMed
高影响因子
Foods
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2022
·Vol. 11
·期 19
·3134
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IF 5.561
Huahua Qiao,Liwei Chen,Jinsong Yang,Wenbo Zhi,Rong Chen,Tianyu Lu,Haisheng Tan,Zhanwu Sheng
Fermentation technology is of great significance for food preservation; through fermentation, while extending the shelf life of food, it can also improve the sensory quality of food and increase its nutritional value. Compared with natural fermentation, the use of specific microorganisms for fermentation can be used to determine the direction of fermentation. Therefore, in order to better explore the effect of bacterial community structure on the quality of sugarcane juice naturally fermented and inoculated with different lactic acid bacteria in the fermentation process, the most suitable method for sugarcane juice fermentation was found, which provided a theoretical basis for the safe production of fermented sugarcane juice. In this experiment, the sugarcane juice was treated differently and divided into four groups: natural fermentation, fermentation withLactobacillusHNK10, fermentation withLactococcusHNK21, and HNK10 + HNK21 compound fermentation. The changes in bacterial community structure of different treatments of sugarcane juice during fermentation were analyzed by high-throughput sequencing technology, and the quality change of different treatments of sugarcane juice during fermentation was analyzed by high-performance liquid chromatography, and the change in its bacteriostatic ability was explored. The results showed that after the sugarcane juice treated withLactobacillusHNK10 was fermented at 37 °C for 48 h, the final fermented sugarcane juice had a large abundance of lactic acid bacteria and high-quality and strong antibacterial activity. Conclusions: changes in the bacterial community structure during the fermentation of sugarcane juice affect the formation of organic acids and the change of bacteriostatic ability and directly determine the quality and shelf life of fermented sugarcane juice.Keywords:sugarcane juice;fermentation;high-throughput sequencing;high-performance liquid chromatography
研究领域:
食品科学
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微生物学
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DOI
PubMed
高影响因子
Analytical Methods
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2015
·Vol. 7
·期 18
·7625-7630
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IF 1.821
Yi Li,Jinhui Zhou,Xiaofeng Xue,Liming Wu,Lanzhen Chen,Jinzhen Zhang,Shuming Yang
An optimization method of NBD-F derivatization has been developed for the determination of proline in honey using high performance liquid chromatography (HPLC) with fluorescence detection. To determine the free proline in different samples of honey, ultrasound extraction was performed using 0.1 M borate buffer solution as the extraction solvent. Chromatographic separation was performed on a XDB C18 column (150 × 4.6 mm i.d.) using 0.1 M sodium acetate buffer solution (pH 7.2) : methanol : tetrahydrofuran (900 : 95 : 5, respectively, v/v/v) as mobile phase A and methanol as mobile phase B at a flow rate of 1.0 mL min−1 with a run time of 10 min. The assay was linear over a range of 0.15 μg mL−1 to 100.00 μg mL−1 with a lower limit of detection of 3.00 mg kg−1, and recoveries were greater than 90%. The intra-day and inter-day precision was less than 4%. This method was successful in determining free proline in honey, ranging from 80.5 mg kg−1 to 426.4 mg kg−1 in different honey samples.
研究领域:
分析化学
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食品科学
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DOI
高影响因子
RSC Advances
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2017
·Vol. 7
·期 20
·11921-11928
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IF 3.108
Shan Xue,Chaozhan Wang,Yinmao Wei
A magnetic mesoporous carbon material (Fe3O4@C) was fabricated by carbonizing polystyrene grafted polydopamine-coated magnetic nanoparticles. The chemical composition, morphology and magnetism of the mesoporous carbon materials were characterized. The as-prepared magnetic mesoporous carbon was employed for enrichment of chlorophenols from water samples coupled with high-performance liquid chromatography with UV detection (HPLC-UV). Various parameters affecting the enrichment were investigated. A good linearity was obtained in the range of 10–200 ng mL−1 for 2-CP, 4-CP and 2,4-DCP, and 5–200 ng mL−1 for 2,4,6-TCP. The recoveries of CPs were in the range of 84.2–120% with intra- and inter-relative standard deviations (RSD) lower than 11.0%. The proposed method is fast, convenient and environmentally friendly. The results revealed the suitability of Fe3O4@C nanoparticles as adsorbents for enrichment of CPs from environmental water samples.
研究领域:
环境科学
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分析化学
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DOI
高影响因子
危险品化学品经营许可证（带存储）