SENSORS
·
2017
·Vol. 17
·期 1
·80
·
IF 2.677
Changwu Lv,Zhenhong Jia,Jie Lv,Hongyan Zhang,Yanyu Li
N-type macroporous silicon microcavity structures were prepared using electrochemical etching in an HF solution in the absence of light and oxidants. The CdSe/ZnS water-soluble quantum dot-labeled DNA target molecules were detected by monitoring the microcavity reflectance spectrum, which was characterized by the reflectance spectrum defect state position shift resulting from changes to the structures’ refractive index. Quantum dots with a high refractive index and DNA coupling can improve the detection sensitivity by amplifying the optical response signals of the target DNA. The experimental results show that DNA combined with a quantum dot can improve the sensitivity of DNA detection by more than five times.Keywords:N-type porous silicon;quantum dot labeling;QD-DNA;reflectance spectrum
研究领域:
材料科学
·
光学
·
生物传感
·
纳米技术
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DOI
PubMed
高影响因子
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
·
2019
·
IF 3.218
Di Huang,Lingxi Yang,Xing lin Li,Lina Zou,Baoxian Ye
A novel electrochemical sensor was designed and constructed for high-sensitive detection of Taxifolin based on Co 3 S 4 @MoS 2 loaded on reduced graphene oxide (RGO-Co 3 S 4 @MoS 2 ) as modified materials. It’s the first time for Co 3 S 4 @MoS 2 was exploited to constructed electrochemical sensor. Owing to the Co 3 S 4 @MoS 2 providing a huge surface area to enrich more Taxifolin and the excellent electric conductivity of RGO, Taxifolin was detected sensitively with a linear range from 5.0 × 10 −9 to 1.0 × 10 −6 mol L − 1 and limit of detection down to 1.67 × 10 −9 mol L − 1 (S/N = 3). In addition, the sensor also demonstrated strong anti-interference properties in the presence of some metal ions and organic compounds. The proposed method was successfully applied for determination of Taxifolin in traditional Chinese medicine in fructus polygoni orientalis with satisfactory results.
研究领域:
分析化学
·
材料科学
·
药学
·
电化学
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产品关联
DOI
高影响因子
JOURNAL OF PROTEOME RESEARCH
·
2021
·
IF 4.466
Xinrong Jiang,Fengbin Gao,Xiaoming Chen,Yanlan Yu,Guoqing Ding,Jianmin Wu
More and more evidence has proved that urinary metabolites can instantly reflect disease state. Therefore, ultra-sensitive and reproducible detection of urinary metabolites in a high-throughput way is urgently desirable for clinical diagnosis. Matrix-free laser desorption/ionization mass spectrometry (LDI-MS) is a high-throughput platform for metabolites detection, but it is encountered by severe interference from numerous salts in urine samples, because the crystallized urine salt on dried samples could result in poor reproducibility in LDI-MS detection. The present work proposed a tip–contact extraction (TCE) technique to eliminate interference from the urine salt. Vertical silicon nanowire arrays decorated with the fluorinated ethylene propylene film (FEP@VSiNWs) could effectively extract metabolites from the urine sample dropping on its surface. High salt tolerance was observed in the subsequent LDI-MS detection of the metabolites extracted on the tip of FEP@VSiNWs even in the presence of 1 M urea. Stable and reproducible mass spectra for non-target metabolic analysis were obtained in real urine samples with different dilution folds. Urinary metabolites collected from bladder cancer (BC) patients were reliably profiled by the TCE method coupled with negative LDI-MS. Based on this platform, potential metabolic biomarkers that can distinguish BC patients and normal controls were uncovered.
研究领域:
分析化学
·
医学诊断
·
质谱技术
✓ 已验证
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2015
·
IF 4.097
Qing Jin,Yan Li,Jianzhong Huo,Xiaojun Zhao
The phosphorescent 3-mercaptopropionic acid (MPA) capped Mn-doped ZnS quantum dots (MPA-Mn:ZnS QDs)-KMnO 4 hybrid system was developed to be an “off–on” phosphorescent switch based on chemical redox mechanism for detection of glutathione (GSH) in food, wine, and biological samples. By employing KMnO 4 as the quencher to the phosphorescence of MPA-Mn:ZnS QDs, GSH recovered the emission for its determination by the use of its reducibility. The possible mechanism for the restored phosphorescence was elucidated that SO 4 2− oxidized by KMnO 4 on the surface of the Mn:ZnS QDs was reduced to S 2− with the addition of GSH. The phosphorescent hybrid system allowed highly sensitive detection of GSH in aqueous solution with a detection limit of 97 nM and a wide linear range of 0.3–280 μM. The precision for 11 replicate detection of 200 μM GSH was 3.5% (relative standard deviation, RSD). The developed method was applied for the detection of GSH in food, wine, and biological samples with the quantitative spike recoveries from 95% to 104%.
研究领域:
分析化学
·
生物分析
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产品关联
DOI
高影响因子
ANALYTICA CHIMICA ACTA
·
2019
·
IF 5.256
Lei Meng,Chengwu Lan,Zhonghu Liu,Na Xu,Yuqing Wu
It is of great importance to detect chlorotetracycline (CTC) in a highly sensitive and specific way because of its wide distribution in aquaculture and animal husbandry. Herein, we propose a novel ratiometric fluorescence strategy to assay CTC by using bovine serum albumin stabilized gold nanoclusters (BSA-AuNCs). The BSA-AuNCs consisting of 25 gold atoms (Au 25 NCs) display a red emission at 640 nm ( λ ex = 370 nm). In the presence of CTC, a new blue emission at 425 nm is emerged and its intensity dramatically increases with the addition of more the analyte; meanwhile the red emission at 640 nm shows a linear decrease reversely. However, at identical conditions neither the analogues of CTC as tetracycline (TC), oxytetracycline (OTC) or doxycycline (DC) induces similar response of BSA-AuNCs. Such interesting phenomenon is proven related to the conversion from large Au 25 NCs to smaller nanoclusters composing 8 gold atoms (Au 8 NCs), which intrinsically originate from the interaction between CTC and the ligand BSA. Therefore, a ratiometric probe is established to sensitively detect CTC in the wide range (0.2–10 μM) with a low limit of detection (LOD) at 65 nM. In addition, this strategy can also be applied to assay CTC in human serum, showing great promise for practical applications in future.
研究领域:
分析化学
·
环境科学
·
材料科学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2019
·
IF 7.65
Yang Wang,Xueyan Liu,Lei Zhang
The excessive accumulation of sulfonamides (SAs) drugs makes it imperative to develop novel materials for boosting simultaneously efficient removal and precise monitoring of multiple SAs. Herein, three-dimensional hollow flower-like Ni@nitrogen-doped graphitic carbon (3DHFNi@NGC) was designed/fabricated via a facile one-pot hydrothermal route and subsequent pyrolysis. The resultant 3DHFNi@NGC exhibits a unique 3D hollow hierarchical architecture assembled by a layer-by-layer interlacing of corrugated nanosheets subunits, thereby affording numerous interconnected channels, available internal/external surfaces as well as suitable interior cavities. By virtue of its special architecture and in-situ generated N-doped graphitic carbon along with good magnetism, the 3DHFNi@NGC demonstrates superior sorption performance towards SAs, accompanied by high total saturated adsorption capacity, fast sorption rate and easy magnetic recycling. It is noteworthy that as-constructed 3DHFNi@NGC also exhibits high-sensitive/simultaneous detection of trace multiple SAs combined high performance liquid chromatography (HPLC), together with a low detection limit (0.035–0.071 ng mL -1 ) and a broad linear range (0.2–100 ng mL -1 ) as well as high enrichment factors (252 < EFs < 291). These indicate that the smart 3DHFNi@NGC could be a promising candidate for the synchronous remediation and sensitive detection of multiple SAs in aqueous systems, presenting a viable option for sewage treatment and water quality monitoring.
研究领域:
环境科学
·
材料科学
·
分析化学
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2021
·
IF 7.514
Qingyi Wei,Peiyao Zhang,Hongbin Pu,Da-Wen Sun
Trace amounts of 17 β -estradiol (E2) in food and the environment poses a threat to human health, which has created the demand for sensitive analytical methods to detect E2. In this study, a novel fluorescent aptasensor was developed for sensitive detection of E2 based on double-chain hybridization between carbon quantum dots-labelled with E2 aptamer (CQDs-aptamer) and Fe 3 O 4 nanoparticles modified by complementary DNA (Fe 3 O 4 -cDNA). Under the optimal conditions, the aptasensor displayed a good linear range of 10 -11 –10 -6 M for E2 with the coefficient of determination (R 2 ) of 0.996, and a low detection limit of 3.48 × 10 -12 M was obtained. Besides, the aptasensor showed high selectivity and good reproducibility for E2 detection, which was successfully applied to the sensitive detection of E2 in milk as compared with tap water and lake water with satisfactory recoveries from 85.21% to 114.80%, suggesting the great significance of this aptasensor for detecting food contaminants in the food industry.
研究领域:
分析化学
·
环境科学
·
食品科学
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产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2022
·
IF 5.304
Chi Zhang,Gaiping Li,Baoxian Ye,Lina Zou,Weihang Wang,Yanli Ji
Based on ExoIII-assisted nucleic acid circulation and catalytic hairpin assembly (CHA) reaction, a dual-amplification electrochemical biosensors strategy for sensitive detection of tobramycin was presented. When the target was present, the trigger 1 (T1) was released to unfold hairpin DNA (H1), which allowed the ExoIII-assisted nucleic acid circulation to proceed smoothly and create a lot of trigger 2(T2). Following that, the CHA reaction between H2 and H3 proceeded under the catalysis of T2 and produced plenty of H2-H3 complex. Signal probe labeled with ferrocene was immobilized on the electrode ahead by adenine (PolyA). Finally, the H2-H3 complex combined with the probe and the DNA strand labeled with ferrocene (C2) fell off the electrode surface. The above process significantly reduced the electrochemical signal, so the biosensor has a low detection limit of 0.11 nM. Moreover, this biosensor has a satisfactory regenerability.
研究领域:
生物化学
·
分析化学
·
生物技术
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产品关联
DOI
高影响因子
OPTICS AND LASER TECHNOLOGY
·
2025
·
IF 5
Jun Dong,Yixuan Hou,Wei Gao,Chengyun Zhang,Lipeng Zhu,Yongkai Wang,Qingyan Han,Jianxia Qi
Surface-Enhanced Raman Scattering (SERS), with the exceptional single-molecule detection capability and high sensitivity, has become an advanced spectroscopic analysis method in fields such as chemical analysis and biosensing. Three-dimensional (3D) sandwich substrates, due to their unique structural characteristics, have attracted considerable attention for their ability to enhance SERS detection sensitivity. However, the controlled assembly of 3D sandwich substrates with bimetallic nanoparticles remains a challenge. In this paper, a fabrication method for constructing 3D sandwich substrates via a bottom-up approach is proposed. Using a liquid–liquid phase self-assembly method, Au@Ag nanocubes (Au@Ag NCs) were assembled on a polystyrene (PS) microsphere array with monolayer MXene (Ti 3 C 2 T X ) adsorption, successfully constructed an Au@Ag NCs/MXene/PS microsphere substrate. Furthermore, the SERS sensitivity of the substrate was evaluated using Rhodamine 6G (R6G), Crystal Violet (CV), and Aspartame (APM) molecules, and experimental results show that the detection limits are 10 −12 M, 10 −11 M, and 0.0313 g/L (1.06 × 10 −4 M), respectively, with excellent uniformity and stability of the substrate. These findings demonstrate that the constructed 3D substrate exhibits outstanding SERS performance and holds significant potential for applications in environmental pollutant detection and food safety.
研究领域:
分析化学
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纳米生物技术
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环境监测
·
食品安全
·
光谱学
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
ELECTROCHIMICA ACTA
·
2016
·
IF 4.803
Dandi Zhou,Min Wang,Jing Dong,Shiyun Ai
The rapid and sensitive detecting technique for subgroup J of avian leukosis viruses (ALVs-J) needs to be developed as soon as possible in order to not only reduce economic losses but also ensure the food safety. In this paper, a novel electrochemical immunosensor was built based on mesoporous graphitic carbon nitride (mpg-C 3 N 4 ). Mpg-C 3 N 4 was used as the sensor platform to bond with the primary antibodies (Ab 1 ). The compound of thionine and mpg-C 3 N 4 (Th-mpg-C 3 N 4 ) was synthesized for the first time to serve as the electroactive probe as well as the carrier of secondary antibodies (Ab 2 ). Compared to bulk g-C 3 N 4 , mpg-C 3 N 4 possesses larger specific surface area, smaller electrochemical resistance and abundant active sites. So the proposed electrochemical immunosensor exhibited amplified detective signals which realized the sensitive detection of ALVs-J. Under the opitimized conditions, the immunosensor exhibited outstanding analytical performance for the detection of ALVs-J whose titer ranged from 10 2.08 to 10 4.0 TCID 50 /mL (TCID 50 : 50% tissue culture infective dose) with a low detection limit of 120 TCID 50 /mL (S/N = 3). The sensitivity of the immnosensor was 6.15 μA/(TCID 50 /mL). This high sensitive immunosensor also displayed good selectivity, reproducibility and stability. Last but not least, this new strategy may be of great promise for clinic application in the future.
研究领域:
生物医学工程
·
免疫学
·
病毒学
·
分析化学
·
纳米生物技术
✓ 已验证
DOI
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2023
·
IF 12.6
Limin Yang,Heng Guo,Ting Hou,Jingang Zhang,Feng Li
Breast cancer (BC) is the most common cancer in the world and circulating tumor cells (CTCs) are reliable biomarkers for early breast cancer diagnosis in a non-invasive manner. However, effective isolation and sensitive detection of BC-CTCs by portable devices in human blood samples are extremely challenging. Herein, we proposed a highly sensitive and portable photothermal cytosensor for direct capture and quantification of BC-CTCs. To achieve efficient isolation of BC-CTCs, aptamer functionalized Fe 3 O 4 @PDA nanoprobe was facilely prepared through Ca 2+ -mediated DNA adsorption. To further detect the captured BC-CTCs with high sensitivity, multifunctional two-dimensional Ti 3 C 2 @ [email protected] nanozyme was synthesized, which not only possessed superior photothermal effect but also exhibited high peroxidase-like activity for catalyzing 3,3′,5,5′-tetramethylbenzidine (TMB) to produce TMB oxide (oxTMB) with a strong photothermal characteristic, combining with Ti 3 C 2 @ [email protected] to synergistically amplify the temperature signal. Moreover, numerous Ti 3 C 2 @ [email protected] nanocomposites would be selectively attained on the BC-CTCs surface through multi-aptamer recognition and binding strategy, which further enhanced the specificity and facilitated signal amplification . Therefore, direct separation and highly sensitive detection of BC-CTCs was successfully achieved in human blood samples. More significantly, the controlled release of the captured BC-CTCs without affecting cell viability could be straightforwardly realized by a simple strand displacement reaction. Thus, with the distinct features of portability, high sensitivity, and easy operation, the current method holds great promise for early diagnosis of breast cancer.
研究领域:
生物医学工程
·
癌症诊断
·
纳米技术
·
生物传感器
·
分子生物学
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产品关联
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2025
·Vol. 192
·期 8
·1-13
·
IF 5.3
Li Fang,Luo Mingming,Tan Yijun,Liu Ruowei,Xu Longping,Zhao Pei,Wu Zijian
A dual-mode electrochemical and fluorescent sensing platform for the highly sensitive detection of kanamycin (KANA) in milk was constructed, which leveraged the synergistic effects of carbon dots (CDs)-loaded metal–organic frameworks (CDs@Cu/Al-MOF) and Ti 3 C 2 , along with the competitive binding of complementary DNA (cDNA) strands to KANA-specific aptamers. Loading CDs onto Cu/Al-MOF effectively prevented the aggregation of CDs and shielded the fluorescent signals from environmental interference, significantly enhancing signal stability. When a glassy carbon electrode (GCE) functionalized with Ti 3 C 2 and aptamers was immersed in a solution of cDNA-functionalized CDs@Cu/Al-MOF, the aptamers hybridized with the cDNA, anchoring the CDs@Cu/Al-MOF onto the GCE surface. This resulted in enhanced electrochemical signals and weakened fluorescence intensity in the solution. Upon introduction of KANA, the aptamer preferentially bound to KANA and the cDNA/CDs@Cu/Al-MOF detached from the electrode, leading to a decrease in the electrochemical signal and an increase in the fluorescence signal. The developed dual-mode aptasensor enabled highly sensitive detection of KANA within a range of 100 fM-1 μM, with the limit of detection (LOD) as low as 0.5254 fM (electrochemical mode) and 0.4398 fM (fluorescence mode). The dual-mode aptasensor exhibited excellent selectivity, reproducibility, and promising performance in real milk sample analysis. Graphical
研究领域:
分析化学
·
生物传感器
·
纳米技术
·
食品安全
·
电化学
·
荧光光谱学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）