SCIENCE OF THE TOTAL ENVIRONMENT
·
2021
·
IF 7.963
Hong-Tao Wang,Lei Ma,Dong Zhu,Jing Ding,Gang Li,Bing-Jie Jin,Yuan-Hu Shao,Wei-Xin Zhang,Meng-Ya Song,Sheng-Lei Fu
The growing contamination of arsenic and plastics has severely effects on the soil fauna health, including shifts of gut microbiota community. A few studies have focused on effects of microplastics and metal(loid) in soil and fauna gut microbiome. However, the environmental effect of nanoplastics and arsenic on the earthworm gut microbiota, especially on arsenic biotransformation in the gut, remain largely unknown. Here, a microcosm study was performed to explore the effects of nanoplastics and arsenic on the microbiota characteristics in earthworm Metaphire vulgaris gut using Illumina high throughput sequencing, and to investigate changes in the gut microbiota-mediated arsenic biotransformation genes (ABGs) by using high-throughput quantitative PCR. Our results demonstrated that the concentration of arsenic in the earthworm body tissues after exposure to arsenic and nanoplastics was significantly lower from that with arsenic alone exposure. Moreover, the clearly different bacterial community was observed in the soil compared with the earthworm gut, which was dominated by Proteobacteria, Actinobacteria, and Firmicutes at phylum level. Arsenic exposure significantly disturbed bacterial community structure in the earthworm gut, but exposure to nanoplastics did not induce gut microbiota changes. More interestingly, nanoplastics can relieve adverse effect of arsenic on the gut microbiota possibly by adsorbing arsenic. In addition, a total of 16 ABGs were detected, and predominant genes involved in arsenic reduction and transport process were observed in the earthworm guts. In short, this study provides a new picture of the effects of nanoplastics and arsenic on the gut microbiota and arsenic biotransformation in soil fauna gut.
研究领域:
环境科学
·
微生物学
·
土壤生态学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MOLECULES
·
2019
·Vol. 24
·期 8
·1593
·
IF 3.06
Xin-Jun Yu,Chang-Yi Huang,Hong Chen,Dong-Sheng Wang,Jing-Liang Chen,Hui-Juan Li,Xiao-Yan Liu,Zhao Wang,Jie Sun,Zhi-Peng Wang
The traditional biochemical methods for analyzing cellular composition of oleaginous microorganisms are time-consuming, polluting, and expensive. In the present study, an FT-IR method was used to analyze the cellular composition of the marine oleaginous protistAurantiochytriumsp. during various research processes, such as strains screening, medium optimization, and fermentation, and was evaluated as a green, low-cost, high throughput, and accurate method compared with the traditional methods. A total of 109Aurantiochytriumsp. strains were screened for lipid and carbohydrate production and the best results were found for the strains No. 6 and No. 32. The yields and productivities could reach up to 47.2 g/L and 0.72 g/L/h for lipid, 21.6 g/L and 0.33 g/L/h for docosahexaenoic acid (DHA) in the strain No. 6, and 15.4 g/L and 0.18 g/L/h for carbohydrate in the strain No. 32, under the optimal conditions, respectively. These results confirmed potentials of the twoAurantiochytriumsp. strains for lipid, DHA, and carbohydrate productions at industrial scales. The FT-IR method in this study will facilitate research on the oleaginousAurantiochytriumsp., and the obtained two strains for lipid and carbohydrate productions will provide the foundations for their applications in medical, food, and feed industries.Keywords:Aurantiochytriumsp., carbohydrate;DHA;feed;FT-IR;high-throughput
研究领域:
生物技术
·
海洋生物学
✓ 已验证
DOI
PubMed
高影响因子
Inorganic Chemistry Frontiers
·
2023
·Vol. 10
·期 16
·4711-4718
·
IF 7
Jingjing Zhang,Ruqing Wei,Daqing Yang,Ying Wang,Bingbing Zhang
Nonlinear optical (NLO) materials have been applied in many high technology fields. In this work, the first-principles high-throughput screening pipeline (FHSP) has been used for searching NLO materials from the silicate system. 253 silicates collected in the Inorganic Crystal Structural Database (ICSD) have been successfully calculated. Among them, 8 crystals were screened out after two-step evaluation. Finally, 3 compounds were selected for synthesis after comprehensive investigation. The SHG intensities tested using the Kurtz–Perry method of KNbSi2O7, PbZnSiO4, and Na2TiSiO5 are 6.7, 2.9, and 2.8 times that of KDP, respectively. Their UV absorption edges are 243 nm, 276 nm, and 280 nm, respectively. This work provides a full view of silicates as NLO materials and obtains excellent candidates for the next step of development.
研究领域:
材料科学
·
计算化学
·
晶体学
✓ 已验证
DOI
高影响因子
Journal of Pharmaceutical Analysis
·
2025
·
IF 8.9
Yuexiang CHANG,Hao TIAN,Jia-Huan QU,Jiaming YUAN,Rongkai GU,TingTing ZHANG,Jincai WANG,Zhengjin JIANG
The N -terminal domain of influenza viral polymerase (PA N ), a highly conserved region with critical catalytic function related to viral RNA replication and transcription, is considered as a very promising anti-influenza drug target. There is an urgent need for highly efficient and rapid screening methods to identify potential PA N inhibitors (PA N Is) from complex matrices. In this work, a novel high-throughput screening (HTS) platform was established by coupling high performance liquid chromatography and high-resolution mass spectrometry (HPLC-HRMS) with a fluorescence resonance energy transfer (FRET)-based endonuclease activity assay through an at-line nanofractionation system (ANF). The proposed screening platform could rapidly identify potential PA N Is from plant extracts with good sensitivity (baloxavir at the half maximal inhibitory concentration (IC 50 ) could be detected) and reliability ( Z ’ factor of 0.77). This platform was then successfully applied to the screening of potential inhibitors against PA N /PA N I38T from an aqueous extract of Artemisiae Argyi and 17 potential PA N Is were identified. Among them, three compounds (cynarine, isochlorogenic acid B and isochlorogenic acid C) showed comparable inhibitory activity against PA N and even better activity against PA N I38T compared to baloxavir. This study not only established a novel high-throughput ANF-based PA N Is screening platform, but also proved the feasibility to discover PA N Is from complex TCMs, which has a great potential in future anti-influenza drug discovery.
研究领域:
病毒学
·
药物筛选
·
植物药理学
·
生物分析技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Applied Nano Materials
·
2024
·
IF 5.3
Ke Ma,Meng Yin,Xiao-Xue Wang,Chen-Yu Wang,Ke-Zheng Chen,Sheng-Lin Qiao
There is an urgent need for efficient and convenient methods to detect SARS-CoV-2 as the global pandemic continues. Currently, detection of COVID-19 relies primarily on reverse transcription PCR (RT-PCR), a laboratory-based method that is time-consuming. To tackle this challenge, the study proposes an immediate high-throughput detection platform for SARS-CoV-2. The platform uses a gold nanoenzymatic colorimetric assay that focuses on the specific detection of 3CLpro in SARS-CoV-2. The platform has been extensively characterized for stability, sensitivity, detection range, and response time. The colorimetric detection method not only provides easy-to-read results but also allows color readings to be taken using a smartphone, with accuracy comparable to that of large instruments such as a microplate reader. The innovation of this technology lies in the ability to achieve instant high-throughput detection, which makes rapid detection of SARS-CoV-2 feasible. The proposed detection platform has a wide range of potential applications, particularly in the field of virus detection, providing an efficient and reliable solution for managing current pandemics and future disease prevention and control.
研究领域:
病毒学
·
医学检测
·
纳米生物学
✓ 已验证
产品关联
DOI
高影响因子
Polymers
·
2023
·Vol. 15
·期 4
·944
·
IF 5
Chi Jiang,Zhaohui Fei,Yingfei Hou
A reverse osmosis (RO) membrane with a high water permeance and salt rejection is needed to reduce the energy requirement for desalination and water treatment. However, improving water permeance while maintaining a high rejection of the polyamide RO membrane remains a great challenge. Herein, we report a rigid–flexible coupling strategy to prepare a high-performance RO membrane through introducing monoamine with a flexible aliphatic ring (i.e., piperidine (PPR)) into the interfacial polymerization (IP) system of trimesoyl chloride (TMC) and m-phenylenediamine (MPD). The resulted polyamide film consists of a robust aromatic skeleton and soft aliphatic-ring side chain, where the aliphatic ring optimizes the microstructure of polyamide network at a molecular level. The obtained membranes thereby showed an enhanced water permeance of up to 2.96 L·m−2h−1bar−1, nearly a 3-fold enhancement compared to the control group, meanwhile exhibiting an ultrahigh rejection toward NaCl (99.4%), thus successfully overcoming the permeability–selectivity trade-off limit. Furthermore, the mechanism of the enhanced performance was investigated by molecular simulation. Our work provides a simple way to fabricate advanced RO membranes with outstanding performance.Keywords:reverse osmosis;interfacial polymerization;molecular structure;high permeance
研究领域:
材料科学
·
环境工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Foods
·
2022
·Vol. 11
·期 19
·3134
·
IF 5.561
Huahua Qiao,Liwei Chen,Jinsong Yang,Wenbo Zhi,Rong Chen,Tianyu Lu,Haisheng Tan,Zhanwu Sheng
Fermentation technology is of great significance for food preservation; through fermentation, while extending the shelf life of food, it can also improve the sensory quality of food and increase its nutritional value. Compared with natural fermentation, the use of specific microorganisms for fermentation can be used to determine the direction of fermentation. Therefore, in order to better explore the effect of bacterial community structure on the quality of sugarcane juice naturally fermented and inoculated with different lactic acid bacteria in the fermentation process, the most suitable method for sugarcane juice fermentation was found, which provided a theoretical basis for the safe production of fermented sugarcane juice. In this experiment, the sugarcane juice was treated differently and divided into four groups: natural fermentation, fermentation withLactobacillusHNK10, fermentation withLactococcusHNK21, and HNK10 + HNK21 compound fermentation. The changes in bacterial community structure of different treatments of sugarcane juice during fermentation were analyzed by high-throughput sequencing technology, and the quality change of different treatments of sugarcane juice during fermentation was analyzed by high-performance liquid chromatography, and the change in its bacteriostatic ability was explored. The results showed that after the sugarcane juice treated withLactobacillusHNK10 was fermented at 37 °C for 48 h, the final fermented sugarcane juice had a large abundance of lactic acid bacteria and high-quality and strong antibacterial activity. Conclusions: changes in the bacterial community structure during the fermentation of sugarcane juice affect the formation of organic acids and the change of bacteriostatic ability and directly determine the quality and shelf life of fermented sugarcane juice.Keywords:sugarcane juice;fermentation;high-throughput sequencing;high-performance liquid chromatography
研究领域:
食品科学
·
微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Nature Communications
·
2025
·Vol. 16
·期 1
·1-9
·
IF 15.7
Zhang Xiu-Feng,Zhang Shi-Nan,Zhang Zhao,Leng Bing-Liang,Lu Kai-Yuan,Chen Jie-Sheng,Li Xin-Hao
Deuterated organic compounds have gained significant attention due to their diverse applications, including reaction mechanism studies, probes for metabolism and pharmacokinetics, and raw materials for labeled compounds and polymers. Conventional reductive deuteration methods are limited by the high cost of deuterium sources (e.g., D₂ gas) and challenges in product separation and D₂O recycling. Electrochemical deuteration using D₂O is promising, but existing methods still suffer from low Faradaic efficiency (FE) and high separation costs. Herein, we report a deuterium ion diffusion-based all-solid electrolyser, featuring a RuO₂ anode for D + generation from pure D 2 O and a Pd/nitrogen-doped carbon-based liquid diffusion cathode (Pd δ+ /NC LDC) with tunable electron deficiencies Pd δ+ /NC to enhance selective deuteration. This system achieves over 99% selectivity for deuterated benzyl alcohol with a FE of 72%, and demonstrates broad applicability for the deuteration of aldehydes, ketones, imines, and alkenes with high FE and selectivity. Moreover, the Pd δ+ /NC-based electrolyser can achieve ten-gram-scale production of deuterated benzyl alcohol over 500 hours, showcasing its potential for high-throughput, solvent-free deuteration reactions in practical applications.
研究领域:
有机化学
·
药物化学
·
合成化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Foods
·
2025
·Vol. 14
·期 10
·1660
·
IF 5.1
Rong Chen,Lan Chen,Mingyue Du,Qiaozhen Guo,Ciping Zhong,Jing Zhang,Xiaoqin Yu
A high-throughput method for the determination of a variety of chemical hazards in poultry muscle and egg samples was established via ultra-performance liquid chromatography–tandem triple quadrupole mass spectrometry (UPLC–QqQ-MS). The sample preparation procedure was developed based on this quick, easy, cheap, effective, rugged, and safe (QuEChERS) method and validated for 280 chemical hazards potentially present in poultry products. The target compounds in poultry samples were extracted with a 1% formic acid–acetonitrile solution (15:85,v/v), and the metal ions in the matrix were chelated by adding ethylenediaminetetraacetic acid disodium salt (Na2EDTA). The supernatant was purified using Enhanced Matrix Removal (EMR) lipid sorbent. Chromatographic gradient separation was performed on an ACQUITY UPLC BEH C18 (2.1 mm × 100 mm, 1.7 μm) column with multiple reaction monitoring (MRM) under both negative- and positive-ion mode. Internal standard calibration or matrix-matched calibration was used for the quantitation. The results showed that good linearity was achieved for each target compound with correlation coefficients (R2) ≥ 0.99. The limits of detection (LODs) ranged from 0.05 to 10 µg/kg, and the acceptable limits of quantification (LOQs) were determined to be 0.1–20 µg/kg for all 280 compounds. Approximately 90% of the target compounds exhibited mean recoveries ranging from 60% to 120%, with relative standard deviations (RSDs) within 16.2%. This method can be used for the high-throughput rapid detection of prohibited drug residues in poultry eggs due to its easy operation and high accuracy. It was applied in real sample detection, and 43 chemicals including metronidazole were found in 211 poultry samples, with a concentration range of 0.11–638 μg/kg.
研究领域:
食品科学
·
分析化学
✓ 已验证
DOI
PubMed
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2024
·
IF 3.8
Sun Zhi,Wang Fangfang,Li Wenxuan,Ren Ruobing,Zhou Peipei,Jia Qingquan,Zhao Lingguo,Chen Di,Zuo Lihua
Therapeutic drug monitoring is essential for ensuring the efficacy and safety of medications. This study introduces a streamlined approach that combines pipette-tip solid-phase extraction (PT-SPE) with matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS), facilitating rapid and high-throughput monitoring of drug concentrations. As a demonstration, this method was applied to the extraction and quantification of antidepressants in serum. Utilizing Zip-Tip C18, the method enabled the extraction of antidepressants from complex biological matrices in less than 2 min, with the subsequent MALDI-MS analysis yielding results in just 1 min. Optimal extraction recoveries were achieved using a sampling solution at pH 9.0 and a 10 μL ethanol desorption solution containing 0.1% phosphoric acid. For MALDI analysis, 2,5-dihydroxybenzoic acid was identified as the most effective matrix for producing the highest signal intensity. The quantification strategy exhibited robust linearities ( R 2 ≥ 0.997) and satisfactory limits of quantification, ranging from 0.05 to 0.5 μg/mL for a suite of antidepressants. The application for monitoring dynamic concentration changes of antidepressants in rat serum emphasized the method’s efficacy. This strategy offers the advantages of high throughput, minimal sample usage, environmental sustainability, and simplicity, providing ideas and a reference basis for the subsequent development of methods for therapeutic drug monitoring. Graphical Abstract
研究领域:
药物分析
·
临床医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
·
2022
·
IF 4.52
Qiuyu Lin,Jiannan Sun,Yuanchao Wang,Meiying Ye,Heyong Cheng
Due to endogenous existence and hazardous properties, the quantification of fatty and aromatic aldehydes in food matrices is indispensable to food safety. Current analytical methodologies for aldehydes in food suffer from several drawbacks (typically complicated pretreatment, long analysis time and low analytical throughput, low sensitivity, etc.). In this work, a highly sensitive, high-throughput and sample pretreatment-free method for aldehyde analysis was developed to address the above problems, where in situ extraction and derivatization of aldehydes with hydrazines to hydrazones were integrated into reactive paper spray ionization mass spectrometry (reactive PSI-MS) considering weakly ionizable properties of aldehydes. Four aldehydes, formaldehyde, acetaldehyde, propionaldehyde and benzaldehyde were reacted with dansyl hydrazine in 1.5 % v/v HAc acidified methanol on the qualitative fast-speed filter paper for 5 min, and then a voltage (+4 kV) was applied to initiate paper spray for mass spectrometry detection with internal calibration by dansyl amide. Once the completion of MS analysis , the glass plate was moved to introduce the second triangular tip towards the MS inlet (5 mm apart) for next trial, achieving high-throughput reactive PSI-MS analysis (60 h −1 ). The four aldehydes showed good linearity in the concentration range from 2 to 150 μM with low detection limits of 0.03–0.15 μM. The method was applied to direct quantification of four aldehydes in 15 kinds of foods such as vegetables, fruits and meat products. The proposed method has the advantages of simple operation, high efficiency and high sensitivity, making it attractive to routine analysis of aldehydes in food, environmental and biological samples.
研究领域:
分析化学
·
食品安全
·
质谱分析
✓ 已验证
DOI
高影响因子
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2020
·Vol. 21
·期 11
·3939
·
IF 4.556
Xiaoyu Li,Zhifeng Shi,Lei Liu,Guanglin Zhu,Jianhua Zhou,Xuetao Shi,Yingjun Wang
Calcium phosphates (CaP) represent an impressive kind of biomedical material due to their excellent biocompatibility, bioactivity, and biodegradability. Their morphology and structure highly influence their properties and applications. Whilst great progress has been made in research on biomedical materials, there is still a need to develop a method that can rapidly synthesize and screen micro/nanosized biomedical materials. Here, we utilized a microarray screening platform that could provide the high-throughput synthesis of biomedical materials and screen the vital reaction conditions. With this screening platform, 9 × 9 sets of parallel experiments could be conducted simultaneously with one- or two-dimensions of key reaction condition gradients. We used this platform to establish a one-dimensional gradient of the pH and citrate concentration and a two-dimensional gradient of both the Ca/P ratio and pH to synthesize CaP particles with various morphologies. This screening platform also shows the potential to be extended to other reaction systems for rapid high-throughput screening.Keywords:microfluidics;high-throughput;concentration gradient;calcium phosphates
研究领域:
材料科学
·
生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）