A CoFe 2 O 4 /MEG solid-phase extraction combined with high performance liquid chromatography was used for determination of 8 biogenic amines (Bas) in canned Spanish mackerel products. A new comprehensive evaluation system, including dietary exposure, food safety index, chemical quality index and biogenic amine index, was used for investigating the pollution distribution, exposure and health assessment of BAs in four sea areas/provinces of China. The pollution levels in the Yellow Sea-Jiangsu, Bohai Sea-Shandong, Yellow and Bohai Sea-Liaoning regions were equivalent, with ∑8BAs equalling 34.7–36.5 mg/kg, while in the South China Sea-Guangdong region ∑8BAs was lowest at 18.9 mg/kg. The concentration range of ∑8BAs and ∑4BAs were ND (not detected)-113 mg/kg and ND-80.8 mg/kg, respectively. All results showed that the main components of BAs were putrescine, histamine, and spermine, with detection rates ranging from 23.1% to 42.3%. The dietary exposure level range of 8 BAs was 85.8–266 μg∙kg −1 ∙d −1 , IFSc was 0.00800–0.0248, CQI values range in the four regions was 1.21–3.93, with a mean CQI of 1.98. The total value of BAI ranges ND-0.840 mg/kg, with a mean BAI of 20.3 mg/kg. Statistical analysis showed that the overall health risk of BAs exhibits different trends in terms of gender, age, and region.
In this work, we demonstrate a novel solid-state electrochemiluminescence (ECL) sensor based on the Ru(bpy) 3 2+ @terbium-guanosine monophosphate infinite coordination polymer network ((Ru(bpy) 3 2+ @Tb-GMP ICPn). Comparing with the traditional luminescence of Ru(bpy) 3 2+ observed in a liquid system, the proposed method of encapsulating Ru(bpy) 3 2+ into ICPn for immobilization greatly improves the ECL signal and efficiency, which is attributed to the unique porous structure and large specific surface area of ICPn. Moreover, the solid-state Ru(bpy) 3 2+ ECL sensor has good biocompatibility and low toxicity. Taking histamine (HA) as a detection model, a good linear relationship between ECL intensity and logarithm of HA concentration was obtained with a low detection limit of 17 nM, and satisfactory results were obtained for detecting HA levels in fish samples as well. The proposed solid-state Ru(bpy) 3 2+ ECL sensor has great application prospects in the safety of food.
Xuan Qi,Wei-Feng Wang,Jun Wang,Jun-Li Yang,Yan-Ping Shi
A highly selective colorimetric detection method for putrescine on the basis of an optimized derivatization reaction was established. With the aids of o -phthalaldehyde (OPA) and thioglycolic acid (TGA), putrescine was derived to become red derivatives (PUT-RD), the form that can be detected qualitatively and quantitatively by visual inspection and UV-vis spectrophotometer at λ max of 490 nm, respectively. Key parameters affecting the experiment were investigated by one-factor-at-a-time and response surface analysis. At the optimal condition, this colorimetric method achieved good linearity at putrescine concentrations ranging from 0.8 to 200 μM with detection limit of 0.44 µM. The method also has good selectivity when common amino acids and inorganic ions, as well as ethylenediamine, 1,3-propanediamine, 1,5-pentanediamine, and 1,6-hexanediamine were used as interferences. The established colorimetric method was successfully employed for the detection of putrescine in 10 commercial fish products.
危险品化学品经营许可证（带存储）