FOOD CHEMISTRY
·
2019
·
IF 5.399
Na Sun,Yixing Wang,Zhijie Bao,Pengbo Cui,Shan Wang,Songyi Lin
Phosphorylation could improve functional characteristics of proteins/peptides, and might be used in the functional improvement of herring egg peptides owing to their enriched phosphorylation sites. The present study aimed to study the effect of phosphorylation on calcium-binding ability of herring egg peptides, and investigate the conformational structure and intermolecular forces of herring egg phosphopeptides (HEPPs)-calcium complex. The HEPPs were found to be superior in calcium-binding activities, as compared to the non-phosphorylated variant. This finding might be attributed to the interaction between calcium ions and the introduced phosphate groups of HEPPs. Calcium favored the formation of β-sheet structure on the HEPPs and induced structural folding, thus assembling into spherical nanoparticles. The conformation of HEPPs-Ca nanoparticles was formed and stabilized mainly by hydrophobic interaction, hydrogen bonds and electrostatic interaction.
研究领域:
食品科学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
VIRUS RESEARCH
·
2022
·
IF 6.286
Bo-Yang Li,Jia-Cheng Qin,Yu-Feng Shen,Fei Yang,Tao Wang,Fei Ling,Gao-Xue Wang
Micropterus salmoides rhabdovirus (MSRV) has a high mortality rate and causes huge economic losses to the aquaculture industry. In this study, we identified that ursolic acid (UA) had antiviral efficacy against MSRV in vitro and in vivo . The results showed that UA inhibited MSRV replication in grass carp ovary (GCO) cells with a half-maximal inhibitory concentration (IC 50 ) of 5.55 μM, reduced viral titers and decreased cytopathic effects (CPE). Mechanistically, UA does not directly damage viral particles. On the other hand, UA inhibits MSRV replication by altering viral binding and release. Furthermore, pre- and post-treatment assays revealed that UA had preventive and therapeutic effects. For in vivo studies, UA could enhance the survival rate of MSRV-infected largemouth bass. Similarly, UA reduced the viral load of MSRV in the heart, spleen and brain at 3, 5 and 7 d post-infection. In conclusion, UA is an effective inhibitor of rhabdovirus in aquaculture.
研究领域:
水产养殖学
·
病毒学
·
药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
TALANTA
·
2017
·
IF 4.162
Guobin Han,Qiaoling Zeng,Zhongwei Jiang,Wenchan Deng,Chengzhi Huang,Yuanfang Li
Phosphospecific enrichment techniques and mass spectrometry (MS) are primary tools for comprehending the cellular phosphoproteome. In this work, a rational and extremely facile route to synthesize the magnetic metal-organic frameworks (mMOFs) was employed and the prepared composite was first utilized as a “bait” for selective enrichment of phosphopeptides. Typically, the mMOFs was synthesized via electrostatic self-assembly between the negatively charged Fe 3 O 4 magnetic nanoparticles (MNPs) and positively charged MIL-101(Fe). The obtained Fe 3 O 4 /MIL-101(Fe) composite possessed well-defined structures, rough surface, highly specific surface area and excellent magnetic property. To demonstrate their ability for enrichment of phosphopeptides, we applied Fe 3 O 4 /MIL-101(Fe) as a “bait” to capture the phosphopeptides from standard protein digestion and practical samples. The enriched phosphopeptides were analyzed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). The MS results show that the Fe 3 O 4 /MIL-101(Fe) exhibits superior enrichment performance for phosphopeptides with low detectable concentration assessed to be 8 fmol, selectivity investigated to be 1:1000 using β-casein/bovine serum albumin mixture and enrichment recovery evaluated to be 89.8%. Based on these excellent properties, the prepared composite was used to enrich the phosphopeptides from tilapia eggs biological samples for the first time. A total number of 51 phosphorylation sites were identified from the digest of tilapia eggs proteins, suggesting the excellent potential of Fe 3 O 4 /MIL-101(Fe) composite in the practical application.
研究领域:
生物化学
·
分析化学
·
蛋白质组学
✓ 已验证
DOI
PubMed
高影响因子
AQUACULTURE
·
2022
·
IF 5.135
Li Bian,Fenghui Li,Pengyan Zhang,Qing Chang,Changlin Liu,Jianlong Ge,Jie Tan,Shengnong Zhang,Siqing Chen
The greenfin horse-faced filefish Thamnaconus septentrionalis is a new marine culture species in East Asia in recent years. It has demersal and adhesive eggs. The artificial fertilization technique of T. septentrionalis has not been established, and commercial hatcheries currently rely on the collection of naturally fertilized eggs. It is impracticable to separate dead eggs from the egg mass , and a large number of waste and faecal matter adhere to the surface of fertilized eggs, causing excess bacterial and fungal loading in eggs incubation tanks. This study aimed to develop an effective method to remove the adhesiveness of fertilized eggs of T. septentrionalis . The effectiveness of tannic acid (0.5%, 0.2% and 0.1%), sodium hypochlorite (0.05%, 0.025% and 0.01%) and alcalase enzyme (4%, 2%, 1% and 0.5%) to eliminate the adhesiveness of T. septentrionalis eggs was evaluated. Eggs were exposed to different deadhesion solutions for up to 20 min, and the number of deadhesive eggs was counted. The results showed that only the alcalase enzyme could effectively remove the adhesiveness of eggs, and the deadhesion efficiency was positively associated with enzyme concentration. The deadhesion rate of the 0.5% alcalase enzyme treatment was more than 70%, while that of the 4%, 2% and 1% alcalase enzyme treatments were more than 90%. The hatching rates were not affected by alcalase enzyme treatment. The hatching rates of the 2% and 1% treatments were higher than those of the control groups. Ultrastructural analysis showed that the surface of eggs had a pore-canal structure, and the zona radiata externa was gradually degraded and finally peeled off during the deadhesion process. Similar to freshwater fishes with adhesive eggs, biochemical analysis showed that the adhesiveness of T. septentrionalis eggs was related to the presence of glycoproteins and mucopolysaccharides in the zona radiata externa. Sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) of zona radiata externa proteins showed that the nonwater-activated eggs, water-activated eggs and adhesive fertilized eggs had same protein pattern, and only small peptide fragments were detected in the deadhesive fertilized eggs. This study provides valuble theoretical and technical support for improvement of hatching technology of filefish.
研究领域:
海洋生物学
·
水产养殖
·
渔业科学
✓ 已验证
产品关联
DOI
高影响因子
AQUACULTURE
·
2022
·
IF 5.135
Yang Hu,Huan Wang,Lei Liu,Jia-yun Yao,Jiong Chen
Micropterus salmoides rhabdovirus (MSRV) is a fish viral pathogen that causes severe disease and huge economic losses in the largemouth bass (M. salmoides ) farming industry in aquaculture. Currently, there is no treatment strategy for MSRV. Thus, anti-MSRV drugs are urgently needed. In this study, it was found that the ethanol extract of Arctium lappa L. had good anti-MSRV activity, and the inhibition rate of G and N protein expression of MSRV was >95%. Further studies showed that arctigenin, a natural component of A. lappa L., could inhibit MSRV infection in a dose-dependent mode on grass carp ovary (GCO) cells. In addition, arctigenin could effectively inhibit the cytopathic changes caused by virus and significantly reduce the titer of virus. The pretreatment and post-treatment experiments showed that arctigenin had preventive and therapeutic effects on MSRV infection. TOA and TOR studies indicated that arctigenin may play an antiviral role in the early stage of virus replication. Interestingly, we found that arctigenin treatment significantly inhibited mitochondrial damage in GCO cells by preserving mitochondrial function and maintaining mitochondrial membrane potential levels (ΔΨm). More importantly, arctigenin was also effective against MSRV infection in largemouth bass. The survival rate of infected fish was increased by 16% and 20% respectively by adding 0.5% and 1% arctigenin in the diet. Additionally, the virus titer of the largemouth bass in the arctigenin treatment group was significantly decreased. In summary, arctigenin is confirmed to be a natural product with the excellent anti-MSRV activity, and its application in the treatment of MSRV is promising in aquaculture.
研究领域:
水产养殖
·
病毒学
·
药理学
·
分子生物学
✓ 已验证
产品关联
DOI
高影响因子
Food Bioscience
·
2023
·
IF 5.2
Yujie Zhu,Xinhong Zheng,Qiaoling Zeng,Rongbin Zhong,Yujing Guo,Feifei Shi,Peng Liang
Large yellow croaker roe phospholipid (LYCRPLs) is a potential antioxidant. In this study, the inhibition of LYCRPLs on fish oil (FO) oxidation was systematically investigated by evaluating peroxide value , acid value, p -anisidine value, thiobarbituric acid value, and volatile compounds in the accelerated oxidation reaction . The results showed that LYCRPLs presented remarkable antioxidant activity and extended FO shelf-life compared with soybean phospholipids. The analysis of volatile compounds elucidated that LYCRPLs mainly inhibited the formation of aldehyde volatile compounds and thus improved the oxidative stability of FO. Moreover, LYCRPLs could effectively improve the thermal stability of FO. This research provides new insights into LYCRPLs as a promising natural antioxidant in the food industry.
研究领域:
食品科学
·
营养学
·
化学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF FISH DISEASES
·
2022
·Vol. 45
·期 6
·895-905
·
IF 2.58
Bo-Yang Li,Fei Yang,Zhong-Yu Zhang,Yu-Feng Shen,Tao Wang,Liang Zhao,Jia-Cheng Qin,Fei Ling,Gao-Xue Wang
Micropterus salmoides rhabdovirus (MSRV) is an significant pathogen that causes high mortality and related economic losses in bass aquaculture. There is no effective or approved therapy to date. In this study, we evaluated the anti-MSRV effects of 22 quinoline derivatives in grass carp ovary (GCO) cells. Among these compounds, 8-hydroxyquinoline exhibited valid inhibition in decreasing MSRV nucleoprotein gene expression levels of 99.3% with a half-maximal inhibitory concentrations (IC 50 ) value of 4.66 μM at 48 h. Moreover, 8-hydroxyquinoline significantly enhanced a protective effect in GCO cells by reducing the cytopathic effect (CPE). By comparing the anti-MSRV activity of 22 quinoline derivatives, we found that 8-hydroxyquinoline possessed the efficient active site of 8-hydroxyl and inhibited MSRV infection in vitro. For in vivo studies, 8-hydroxyquinoline via intraperitoneal injection exhibited an antiviral effect in MSRV-infected largemouth bass by substantially enhancing the survival rate by 15.0%. Importantly, the viral loads in the infected largemouth bass notably reduced in the spleen on the third days post-infection. Overall, 8-hydroxyquinoline was considered to be an efficient agent against MSRV in aquaculture.
研究领域:
水产养殖
·
病毒学
·
药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Frontiers in Nutrition
·
2022
·
IF 6.59
Ren Zhexin,Yang Fei,Yao Sijia,Bi Lijun,Jiang Guanqin,Huang Ju,Tang Yunping
This study aimed to investigate the immunomodulatory activation of low-molecular-weight peptides from monkfish (Lophius litulon) roe (named MRP) on cyclophosphamide (CTX)-induced immunosuppressed mice. Our results indicated that MRP (100 mg/kg/d BW) could significantly increase the body weight and immune organ index, and improve the morphological changes in the spleen and thymus of mice. These effects subsequently enhance the serum levels of interleukin (IL)-6, IL-1β, tumor necrosis factor (TNF)-α, and immunoglobulin (Ig) A, IgM, and IgG. Furthermore, MRP could also improve CTX-induced oxidative stress, and activate the NF-κB and MAPK pathways in the spleen tissues. The findings reported herein indicate that MRP has a good immunomodulatory activation toward immunosuppressed mice, hence can potentially be developed as an immune adjuvant or functional food.
研究领域:
免疫学
·
药理学
·
生物化学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Food Measurement and Characterization
·
2025
·
IF 3.3
Wang Xiaoyuan,Wang Ashily Liang,Yang Chenyu,Qiu Weiqiang,Li Li,Jiang Yunzi,Zhao Wenbin,Li Feng,Jin Yinzhe
Peptide–iron chelates were prepared, and their chelating properties were explored. High voltage pulsed electric field (PEF) was used to assist in the extraction of phosvitin (Pv) from Mylopharyngodon piceus roe, and the Pv extract was subsequently treated and enzymolyzed to obtain phosphopeptide (PP). After PEF pretreatment and enzymatic hydrolysis, total antioxidant capacity and Fe 2 ⁺-chelating ability of PP increased by 54.45% and 12.06%, respectively. Peptide–iron chelates (PP 8 -Fe) were prepared using PP with the highest antioxidant activity. Chelate structure determination revealed that PP 8 -Fe was rich in amino acids with strong metal ion chelation capabilities, namely Glu, Asp, and Ser, which accounted for 50.91% of the total amino acids. Compared to PP 8 , PP 8 -Fe exhibited a more loosely structured surface at the microstructural level, increased iron content, a shift in secondary structure from random coil to α-helix and β-sheet, an increase in weighted average particle size from 420.20 ± 2.80 nm to 768.50 ± 1.90 nm, an increase in zeta potential from -33.70 ± 0.60 mV to -3.78 ± 0.46 mV, enhanced hydrophobicity, an increase in ΔH, and good gastrointestinal digestion stability. PP 8 has iron chelating sites, including phosphate, carboxyl, carbonyl, amino groups, and sialic acid residues. Our findings provide a theoretical foundation for peptide preparation and enhancement of their bioactivity via PEF pretreatment, while also providing guidance for future research on their chelation mechanisms with metal ions.
研究领域:
食品科学
·
生物化学
·
生物技术
·
分析化学
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2023
·
IF 7.4
Jin Han,Xiangyu Wang,Wenxin Wang,Jianxiong Chen,Bin Xu,Zhenwei Wei
Direct analysis of micro-biopsy samples by mass spectrometry at single-cell level still faces major challenges. In this work, we developed a polarity gradient focusing dip-and-go strategy (PGF-Dip&Go) during induced electrospray ionization mass spectrometry (iESI-MS) analysis for real-time enrichment and spatial separation of compounds such as lipids, alkaloids, fatty amines, and drugs. Compared with direct iESI-MS analysis, enrichment of analytes (enrichment factor of 5.0–100.0) and spatial separation between different analytes were achieved. Owing to the enrichment effect and salt cleanup effect, the sensitivity of PGF-Dip&Go has been improved by 25–10,000 times compared with direct iESI-MS. PGF-Dip&Go has been successfully applied for the analysis of lipids in a 200 pL micro-biopsy section from an individual fish egg. Lysophosphatidylcholine (LPC), phosphatidylcholine (PC), and triglyceride (TG) were significantly enriched and separated according to their polarity differences, proving the potential of PGF-Dip&Go to be a noninvasive and powerful analytical tool for in situ analysis of complex small volumes in the future.
研究领域:
分析化学
·
质谱分析
·
生物医学工程
·
生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）