Toxicity evaluation of MC-LR in different fish organs based on aptamer-recognized SERS tag coupled with magnetic separation
Microcystin-LR (MC-LR) is a strong liver toxin, which will enter the human body by eating MC-LR-contaminated aquatic products. Therefore, it is urgent to explore whether people are exposed to MC-LR-contaminated aquatic products. Herein, a sensing strategy based on aptamer-recognized SERS tag coupled with magnetic separation was developed for ultrasensitive and specific detection of MC-LR in fish organs. This sensor had a dynamic range of 0.1 ng/L to100 µg/L and a limit of detection down to 0.1 ng/L for MC-LR. This ultrasensitive and rapid separation strategy allow us to explore the bioaccumulated amount of MC-LR in different fish organs. The results show that the order of MC-LR content accumulated in different organs of local crucian carps were heart > liver > kidney > meat > spleen. The MC-LR content in fish meat was 1.1–8.5 times of the WHO proposed tolerable daily intake level (TDI), implying the aquatic products in local aquatic market had potential threats to human health.
危险品化学品经营许可证（带存储）