A sensitive electrochemiluminescence (ECL) sensor in solid-state for the detection of tetracycline (TET), based on carboxylated carbon nanotubes modified ZnO (CNT-COOHs/ZnO), was developed in this research. The ZnO, which worked as luminophore here, was an environment-friendly material. Resulted from its tremendously specific surface area, the loading amount of CNT-COOHs increased. The CNT-COOHs played a role of catalyzer to enhance the ECL intensity of CNT-COOHs/ZnO modified glass carbon electrode (GCE) in triethanolamine (TEOA) contained electrolyte. After adding TET in the solution, the ECL intensity significantly quenched. The ΔECL intensity showed a good correlation to the concentration of TET in the range of 1.0 × 10 –8 –1.0 × 10 –4 M and the detection limit achieved to 6.7 × 10 –9 M within a response time of 1.0 min. Furthermore, the proposed CNT-COOHs/ZnO based ECL sensor was applied for the detection of TET in fishpond water and exhibited a good accuracy. We believe our system supplied a potential platform for the detection of TET in real world.
Abuse of antibiotics results in a large number of antibiotics residues in the environment and even causes the problem of “super bacteria”. Therefore, it is crucial to develop a powerful analytic method to monitor antibiotics quickly and simply. Photonic crystal (PC), as a sensing material, has promising application prospects. Herein, we try to use PC to realize pretreatment-free and label-free detection of Ciprofloxacin (CF) through Michael addition reaction. The recognition process is carried out by the Michael addition reaction between the piperazine group of CF and the o-benzoquinone group on the PC. The monodisperse microspheres with o-benzoquinone groups are prepared by polymerization and oxidation and then stacked to form PC. During the detection, the peak intensity of the PC decreases with the increasing CF concentration, and the linear range is from 2 to 512 μg/L. The limit of detection (LOD) is 0.76 μg/L. Furthermore, the PC retains 97% of the initial response after storage in a Petri dish at room temperature for 1 month, which shows that it has good stability. Moreover, CF in fish farming water can be detected directly without any pretreatment and label, and the results are in good accordance with the LC-MS-MS results. This Michael-addition-mediated PC is accurate, easily prepared, cost-efficient, and long-term stable. In addition, it is environmentally friendly, because little organic solvent is needed during both the preparation and the detection.
危险品化学品经营许可证（带存储）