ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
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2017
·Vol. 24
·期 10
·9379-9386
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IF 2.741
Jia Yuyu,Wang Lin,Qu Zhipeng,Wang Chaoyi,Yang Zhaoguang
Three fish species ( Carassius auratus , Pelteobagrus fulvidraco , and Squaliobarbus curriculus ) were collected from Xiang River near Changsha City, Southern China. The concentrations of heavy metals including arsenic (As), cadmium (Cd), copper (Cu), iron (Fe), manganese (Mn), lead (Pb), and zinc (Zn) in the muscle, gill, and liver of three species were determined by the inductively coupled plasma mass spectrometry method. One-way analysis of variance (ANOVA) was introduced to determine the significant variations ( p < 0.05) of heavy metals. Livers were found to accumulate Cd and Cu due to the metallothionein proteins. High levels of Mn and Pb in the gills indicated that the main uptake pathway of these heavy metals was from the water. The carnivorous species, P. fulvidraco , was found to accumulate the highest levels of toxic elements (As, Cd, and Pb), while relatively high concentrations of nutrient elements (Cu, Fe, Mn, and Zn) were accumulated in omnivorous species ( C. auratus and S. curriculus ). According to the results of Pearson’s correlation analysis, there were few significant relationships at p < 0.01 level between the concentrations of the analyzed elements and the fish sizes. The results of risk assessment indicated that exposure to the toxic heavy metals from fish muscle consumption posed no non-carcinogenic health risk to local inhabitants.
研究领域:
生态毒理学
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环境科学
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
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2022
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IF 9.231
Jiawen Ji,Changsheng Li,Bingjie Zhang,Wenjuan Wu,Jianli Wang,Jianhui Zhu,Desheng Liu,Rumin Gao,Yongqiang Ma,Sen Pang,Xuefeng Li
N-(1,3-dimethylbutyl)-N′-phenyl-p-phenylenediamine (6PPD) and N-(1,3-dimethylbutyl)-N′-phenyl-p-phenylenediamine-quinone (6PPDQ) can pose a threat to human health through the food chain because of their ubiquitous presence in the environment and the biotoxicity on organisms. In this work, we developed modified QuEChERS methods coupled with high performance liquid chromatography tandem mass spectrometry (HPLC/MS-MS) to explore whether 6PPD and 6PPDQ are present in fish and honey. The proposed methods showed acceptable limits of quantification (0.00043-0.001 mg/kg), linearity (R 2 > 0.99), recovery (73.3%-108.3%), matrix effect (70.4%-95.6%) and repeatability (RSD < 8.4%). Accordingly, 6PPD and 6PPDQ have been discovered in snakehead , weever and Spanish mackerel fish, while none of which have been detected in the honey samples. The results of our work contributed to increasing public attention to 6PPD and 6PPDQ in agricultural products and provided important reference for the analysis of them.
研究领域:
食品科学
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环境科学
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分析化学
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产品关联
DOI
PubMed
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
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2019
·
IF 5.589
Dongdong Zhang,Yaozong Cui,Hanghai Zhou,Cheng Jin,Xinwei Yu,Yongjiu Xu,Yanhong Li,Chunfang Zhang
In this study, the occurrence and distribution of microplastics in artificial reefs around the Ma’an Archipelago, a national marine ranching area in China, were investigated. The abundance of microplastics ranged from 0.2 ± 0.1 to 0.6 ± 0.2 items L −1 in surface water, 30.0 ± 0.0 to 80.0 ± 14.1 items kg −1 dry weight in the sediment, and 2.3 ± 1.5 to 7.3 ± 3.5 items individual −1 in fish. Most of the detected microplastics were fiber-shaped, blue or transparent, and smaller than 1 mm. Polyethylene, polypropylene, and poly(ethylene:propylene:diene) copolymer were the most abundant polymer types in the surface water samples, whereas cellophane was dominant in the sediment and fish. The appearance of microplastic pollution around the artificial reefs could be attributed mainly to the activities of the fisheries in the area, whereas the microplastic ingestion by fish was affected by the extent of microplastic contamination of the sediment. The results highlight the widespread presence of microplastics in the water, sediment, and biota of the artificial reefs around the Ma’an Archipelago, thereby improving understanding of the environmental risks posed by microplastics to marine artificial reef ecosystems and fisheries in general.
研究领域:
海洋生态学
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环境科学
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产品关联
DOI
PubMed
高影响因子
FOOD CONTROL
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2025
·
IF 6.3
Chanchiang Liang,Siyang Chen,Wenxiu Zhu,Hanzheng Dou,Xiaodong Xia
The antimicrobial efficacy of punicalagin against foodborne pathogens has been extensively studied, yet its impact on spoilage microorganisms remains poorly characterized. This study aimed to evaluate punicalagin's inhibitory effects on Pseudomonas fluorescens and explore its potential application in tilapia preservation. Growth curve analysis showend that punicalagin effetively inhibited the growth of Pseudomonas fluorescens . In addition, punicalagin triggered intracellular potassium ion efflux and leakage of nucleic acids and protein. Scanning electron microscopy revealed compromised cell membrane integrity after punicalagin treatment. Furthermore, punicalagin exposure reduced intracellular ATP levels and elevated reactive oxygen species accumulation. Concurrently, it effectively suppressed biofilm formation and destroyed preformed biofilms. In tilapia spiked with Pseudomonas fluorescens , punicalagin markedly attenuated spoilage progression, as evidenced by diminished protein degradation and lipid oxidation. These collective findings indicate that punicalagin exhibit antimicrobial and anti-biofilm activities against Pseudomonas fluorescens , highlighting its potential as a natural preservative for aquatic products.
研究领域:
食品科学
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微生物学
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水产品加工
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产品关联
DOI
高影响因子
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
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2021
·
IF 4.556
Qin Hu,Yikun Cui,Longfei Zhang,Minjie Qian,Lixia Xiao,Ming Yang,Zhen-Quan Yang,Shengqi Rao,Xiaojuan Gong,Jie Han
In this work, a novel fluorescence method based on an orange-emissive carbon dots (O-CNDs) was for the first time reported for the rapid and ultrasensitive determination of malachite green (MG). The O-CNDs were simply produced via a one-pot hydrothermal treatment of rhodamine B. The detection mechanism was based on the interaction between O-CNDs and MG, where the fluorescence of O-CNDs was suppressed by MG via a combined effect of inner filter effect (IFE) and dynamic suppressing. Under the optimum sensing conditions, the O-CNDs were found to display rapid reaction time (1.0 min), high selectivity and excellent sensitivity with two linear concentration ranges of 0.01–0.1 and 0.1–10.0 μmol L −1 , and the corresponding detection limits were as low as 0.08 and 0.93 nmol L −1 , respectively. The developed fluorescence method was successfully applied for the determination of MG in fish samples, including silver carp, blackfish, blunt-snout bream, common carp and grass carp, with satisfactory results; the recoveries were in a range of 96.0–104.8 % and the relative standard deviations (RSDs) below 4.54 %. The results of this study validated the great potential of O-CNDs as an effective fluorescence sensing agent for MG monitoring in aquatic samples.
研究领域:
分析化学
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环境科学
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DOI
高影响因子
FOOD CONTROL
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2024
·
IF 5.6
Xia Xu,Xin Liu,Yixing Qiu,Yufan Song,Xuxia Zhou,Yuting Ding
The accurate determination of histamine in fish is imperative for food safety, given the toxic nature of this biogenic amine . This study presents an innovative colorimetric aptasensor for the ultrasensitive detection of histamine in fish, utilizing nanozymes based on magnetic metal-organic frameworks coated with Pt nanoparticles (Fe 3 O 4 @MOF@Pt), which exhibit high peroxidase-like catalytic activity, magnetism, and superior optical properties. The surface of Fe 3 O 4 @MOF@Pt nanozymes were modified with DNA strands complementary to the histamine aptamer , creating effective colorimetric probes. In the presence of histamine, these colorimetric probes compete with histamine molecules for aptamer binding on the enzyme plate. Color development is triggered by adding a TMB and H 2 O 2 substrate solution to the enzyme plate. This competitive binding-based aptasensor allows for rapid and accurate histamine quantification within a linear range of 10 pM to 10 μM, and a notable detection limit of 6.23 pM. Furthermore, the sensor demonstrated excellent specificity, distinguishing histamine from l -histidine and other biogenic amines. Tested on mackerel samples, the aptasensor showed spiked recoveries of 91.4%–109.6%, validating its practical applicability. This sensor offers an ultrasensitive, specific, and reusable tool for histamine detection, representing a significant advancement in ensuring the safety of fish products.
研究领域:
食品安全
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生物传感
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纳米技术
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产品关联
DOI
高影响因子
Sustainable Materials and Technologies
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2025
·
IF 9.2
Qipu Xin,Ruihao Niu,Zhaojing Huang,Jing Yu,Qihe Chen,Donghong Liu,Enbo Xu
Microcarriers (MCs) play a crucial role in promoting cells to expand in culture systems for the industries as regenerative medicine products and cell-derived alternative proteins. However, high-performance and biosafe MCs are still urgently needed for cell scale-up expansion under the dynamic shearing environment of bioreactor and pipeline. Here, gelatin was used which is of high biocompatibility and edibility as MC matrix, by TGase-induced crosslinking in combination with emulsification method for piscine satellite cells (PSCs) cultivation. MCs cultivation conditions were optimized in the spinner flasks (6000 MCs/mL, 8:1 ratio of cells to MCs, 50 rpm speed), to achieve about 5 fold of PSCs on Day 9. To further increase the proliferation efficiency, solid MCs were modified to porous MCs through ice templating method, which could lead to ∼6.32 proliferation multiple on Day 9 with high-efficiency differentiation. Also, transcriptome analysis showed that the genes related to cell cycle and DNA replication were obviously upregulated in the MCs groups in comparison to the 2D cultivation group of PSCs. Collectively, these findings demonstrate the ability of porous MCs in realizing large-scale cell expansion and even differentiation.
研究领域:
细胞培养
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再生医学
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生物工程
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产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
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2018
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IF 5.667
Yangxia Han,Yonglei Chen,Juanjuan Liu,Xiaoying Niu,Yunxia Ma,Sudai Ma,Xingguo Chen
Silicon nanoparticles (SiNPs) have attracted increasing attentions due to their unique properties and related applications. However, previously reported SiNPs generally showed emission band only in the blue- or green-light regions, which limited their applications in biochemical samples. So far, synthesis of longer wavelength emissive SiNPs remains a challenge. In this paper, the yellow-emitting fluorescent SiNPs were prepared via a facile room temperature procedure, which avoided heating and pressurizing operation, multistep and time-consuming post-modified procedures. The obtained SiNPs displayed excellent water-solubility, pH-stability, salt-tolerance, and photo-stability. Significantly, the fluorescence of SiNPs could be remarkably quenched by crystal violet (CV) via synergistic effect of inner filter effect (IFE) and fluorescence resonance energy transfer (FRET). Based on this phenomenon, a method for the determination of CV was established. Under the optimum conditions, the method showed a wide linearity range from 0.075 to 15.0 μg/mL with a limit of detection of 25 ng/mL. Furthermore, the method was successfully applied to the analysis of CV in fish tissues samples.
研究领域:
生物化学
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分析化学
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产品关联
DOI
高影响因子
FOOD CONTROL
·
2021
·
IF 5.548
Xia Xu,Xiaotian Wu,Yuting Ding,Xuxia Zhou
Histamine determination is of great significance for fish safety, quality and trade. In this work, we developed a novel multicolorimetric biosensor for the simple and rapid visual detection of histamine in fishes. With the help of iodide and molybdate, the hydrogen peroxide generated by the enzymatic reaction of histamine and diamine oxidase enzyme effectively etched the gold nanorods (AuNRs). This etching reaction caused a blueshift of the longitudinal peak of the nanorods, accompanied by an obvious multicolor change from blue-green to purple and red, which could be easily observed by the naked eye. After the optimization of reaction conditions, the linear range of the standard curve obtained from the longitudinal peak blueshift distance of the AuNRs was determined to be 20–100 μM. Attentively, the interference from other analytes is negligible, especially diamines such as putrescine and cadaverine. Furthermore, one can easily distinguish with the naked eye whether the histamine content measured in a fish sample exceeds the limit value of 50 mg/kg using the proposed method. The method is simple and fast, with high specificity and accuracy, and can be successfully applied to determine the histamine content in real fish samples, indicating the potential of its application in the on-site detection of various analytes.
研究领域:
食品科学
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纳米生物学
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分析化学
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产品关联
DOI
高影响因子
MARINE ENVIRONMENTAL RESEARCH
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2024
·
IF 3
Zike Li,Meijing Zhao,Zhihua Feng,Lin Zhu,Qi Sui,Xuemei Sun,Bin Xia
Marine organisms commonly encounter co-stress resulting from the coexistence of microplastics (MPs) and heavy metals pollution in marine environments. Nevertheless, the combined effects and toxicity mechanisms of MPs and heavy metals on marine organisms remain unclear. This study integrated growth, physiological, morphological, and biochemical markers to assess the individual and combined toxicity of polyvinyl chloride MPs (PVC MPs, 1 × 10 4 particles/L) and copper (Cu, 200 μg/L) on marine jacopever ( Sebastes schlegelii ). The results revealed that co-exposure to MPs and Cu had a more detrimental impact on jacopever compared to the single-exposure groups, as evidenced by the enhanced growth inhibition, respiratory stress, and hepatotoxicity . This phenomenon may be attributed to PVC MPs accelerating the accumulation of Cu in jacopever liver. Therefore, peroxidation damage occurred in the co-exposed liver and may result in liver dysfunction . These findings contribute valuable insights into the risks associated with the coexistence of MPs and heavy metal pollution in marine ecosystems .
研究领域:
环境科学
·
毒理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
AQUACULTURE INTERNATIONAL
·
2024
·
IF 2.2
Cui Jun,Zeng Dan,Guan Peipei,Jiang Ning
Exposure to pathogens induces hyperactivate inflammatory immune responses, oxidative stress and even death in fish. Therefore, suppressing excessive inflammatory responses is a means of treating fish diseases. Scutellariae Radix (SR), an herbal medicine, has anti-inflammatory and antioxidant properties. However, the active ingredients of SR that produce a marked effect and their mechanisms are poorly understood. In this study, 32 active ingredients of SR and their 124 target genes associated with anti-inflammatory effect were screened. These target genes in zebrafish were mainly enriched in biological processes associated with response to stress and stimulus. The KEGG enrichment analysis and pathway–pathway network analysis showed that the MAPK signalling pathway and nine other pathways were the key pathways associated with anti-inflammatory effect. A protein–protein interactions (PPI) network of target genes was constructed, and AKT2 and nine other genes were identified as the hub genes by cytoHubba. Combining the above results, we constructed an active ingredient–hub protein–key pathway network, showing that wogonin-AKT2 was the key regulated module. Molecular docking and SPR-based assays were used to investigate the possible interaction of wogonin with the AKT2 protein. The infected zebrafish and grass carp treated with wogonin displayed a higher survival rate and anti-inflammatory and antioxidant effects than the infected fish without wogonin treatment. Dietary wogonin also enhanced grass carp anti-inflammatory and antioxidant effects. These findings further reveal the molecular biological mechanism by which wogonin from SR exerts anti-inflammatory effects and provide a theoretical basis for the prevention of fish diseases.
研究领域:
生物学
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医学
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药理学
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产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2014
·
IF 3.259
Xiaomin He,Ke Dai,Aimin Li,Hao Chen
Residues of eight perfluorinated compounds (PFCs) were investigated in 15 fish samples from the Danjiangkou reservoir and Hanjiang river (Xiangyang and Zhongxiang sections). The total concentrations of PFCs in fish muscles ranged from 2.01 to 43.8 ng g − 1 dry weight. The mean concentration of total PFCs from related muscles showed the following trend in various regions: Zhongxiang section < Xiangyang section < Danjiangkou reservoir. Perfluorooctane sulphonate (PFOS) was the dominant PFC in the fish liver samples from the Danjiangkou reservoir. The calculated hazard ratio (HR) of PFCs, for all fish muscle samples, was less than 1.0, and could be classified at safe levels for the general population. However, yellow croaker fish from the Danjiangkou reservoir and Hanjiang river–Xiangyang section had HRs of 0.2, indicating that frequent consumption of this contaminated fish may pose an unacceptable risk to human health.
研究领域:
环境科学
·
毒理学
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）