Aquaculture Reports
·
2022
·
IF 3.385
Jia Xu,Xinzhou Yao,Yingying Lin,Shuyan Chi,Shuang Zhang,Junming Cao,Beiping Tan,Shiwei Xie
Dietary low-fishmeal (FM) have negative impacts on growth performance and metabolic responses in aquatic animals, while Schizochytrium limacinum have multiple benefits due to its rich contents of docosahexaenoic acid (DHA). However, little information is available on the effects of S. limacinum on Pacific white shrimp ( Litopenaeus vannamei ) in a low-FM diet (LF). An 8-week feeding trial were used to investigate the impacts of S. limacinum on growth performance, as well as morphological changes, biochemical indicators, antioxidant capacity, and transcriptomic profiles of hepatopancreas in shrimp fed a low-FM diet. Compared to LF diet, 0.3% S. limacinum (LFLD) diet significantly decreased the specific growth rate (SGR), and did not affect the feed conversion rate (FCR) and weight gain rate (WGR); whereas 0.6% S. limacinum (LFHD) diet did not affect the SGR, FCR and WGR. Histological structures of hepatopancreas showed that LFLD and LFHD treatments reduced the number and size of secretory cells and increased the number of storage cells, compared to LF group. LFLD and LFHD diets significantly decreased the concentrations of triglyceride and total cholesterol, significantly increased the levels of glutathione peroxidase and total antioxidative capacity, while did not affect the levels of malondialdehyde and superoxide dismutase. A total of 135 differential expression genes (DEGs) were identified in LFLD group and 108 DEGs were identified in LFHD group (both compared to LF group). Administration of S. limacinum altered the transcriptomic profiles, while LFHD induced a pattern similar to that of LF. LFLD and LFHD treatments mainly affected the 9 KEGG metabolic pathways, including “Arachidonic acid metabolism” and “Glutathione metabolism”. In conclusion, LFLD treatment impaired the growth performance of shrimp, while LFHD treatment did not affect it; and both treatments improved the morphological structure, lipid accumulation and antioxidant capacity of hepatopancreas.
研究领域:
水产养殖学
·
营养学
·
分子生物学
✓ 已验证
产品关联
DOI
高影响因子
AQUACULTURE
·
2013
·
IF 2.009
Shi-wei Xie,Li-xia Tian,Yan Jin,Hui-jun Yang,Gui-ying Liang,Yong-jian Liu
An 8-week feeding trial was conducted to evaluate the optimum dietary glycine level in low fishmeal diet for juvenile Pacific white shrimp , Litopenaeus vannamei . Six practical diets (44.4% protein, 8.8% lipid) were formulated to contain graded levels (2.26, 2.33, 2.44, 2.58, 2.67 and 2.74%) of l -glycine. Each diet was randomly assigned to triplicate groups of 30 shrimps (approximately 0.61 g) and the shrimps were fed 4 times a day to apparent satiation. The results indicated that the weight gain and specific growth rate were significantly increased with the supplementation of glycine. The highest weight gain and specific growth rate were observed at 2.56% dietary glycine, and then decrease with a further increase in dietary glycine. Hepatosomatic index , protein productive value and condition factor were also significantly influenced by the dietary glycine levels and show a similar trend to those of growth performance. There were no significant differences observed in feed efficiency , protein efficiency ratio and survival among all treatments. Mg, Ca and Fe concentrations in whole body were significantly increased with the supplementation of glycine. Non-essential amino acid, essential amino acid and arginine ratio in muscle were significantly affected by the dietary glycine levels. Dietary glycine levels higher than 2.44% significantly increased the survival of shrimps after 2 h and 3 h of acute salinity change, and T-SOD activity and Na + /K + -ATPase activity in hematological and hepatopancreas before and after acute salinity change are also significantly affected by the dietary glycine levels. Analysis by second-order regression of weight gain indicated that the optimal dietary glycine level was 2.54% of the dry diet (5.7% of the dietary protein and 6.66% of the dietary amino acids).
研究领域:
水产养殖
·
营养学
·
海洋生物学
✓ 已验证
DOI
高影响因子
Aquaculture Reports
·
2021
·
IF 3.216
Shiwei Xie,Dan Wei,Lixia Tian,Yongjian Liu
Bile acid metabolism in aquatic animals usually is suppressed when fish meal (FM) is replaced by plant proteins. An eight weeks feeding experiment was conducted to evaluate the effects of dietary chenodeoxycholic acid (CDCA) supplementation on growth, immune response and intestinal health of Penaeus monodon fed a low FM diet. Three diets were formulated: high FM (25 %) diet (HF), low FM (15 %) diet (LF), and low FM diet supplemented with 0.1 % CDCA (LFC). Shrimp fed Diet LF showed lower weight gain and feed efficiency than those fed Diet HF. Low dietary FM up-regulated the mRNA level of xbp1 , and down-regulated the mRNA levels of sod , iap and NF-κB signaling pathway-related genes ( traf6 , tube ). Dietary supplementation of CDCA up-regulated the expression of relish and anti-oxidative-related genes ( sod and cat ) in the hepatopancreas and midgut. Low dietary FM impaired the intestinal fold and induced the endoplasmic reticulum swollen of intestinal epithelial cell, dietary supplementation of CDCA alleviated this problem. The intestinal microbiota diversity was lower in shrimp fed Diet LF than those fed HF. Dietary supplementation of CDCA in a low FM diet increased the intestinal microbiota diversity by decreasing the relative abundance of dominant phylum Proteobacteria. Low dietary FM increased the relative abundance of a dominant pathogenic bacterium ( Vibrio ), which was decreased by the supplementation of CDCA. These results indicated that dietary supplementation of CDCA in a low FM diet improved the growth performance, immune response and intestinal health of shrimp.
研究领域:
水产养殖学
·
营养学
·
分子生物学
✓ 已验证
DOI
高影响因子
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
·
2018
·
IF 1.684
Shiwei Xie,Dan Wei,Peng Yin,Lu Zheng,Tianyu Guo,Yongjian Liu,Lixia Tian,Jin Niu
An 8-week feeding trial was conducted to evaluate the effect of fish-meal replacement on growth performance, protein synthesis and immune response of juvenile Pacific white shrimp, Litopenaeus vannamei reared at low salinity (7‰). Five isonitrogenous and isolipidic diets were formulated to contain graded levels (25, 20, 15, 10 and 5%) of fish-meal. High quality alternative solutions were performed, crystalline amino acids, phytase, mannan oligosaccharides and some micro-nutrients were supplemented in the low fish-meal diets. Each diet was randomly assigned to triplicate tanks, each tank with 30 shrimp (mean weight 0.3 g), the shrimp were fed 3 times a day. Weight gain and survival were decreased with the decreasing dietary fish meal levels. When dietary fish-meal decreased, the gene expression of TOR, Raptor and eIF4E2 in hepatopancreas were decreased with the decreasing fish meal levels, eIF4E2 in intestine was decreased while 4E-BP was increased with the decreasing fish meal levels. The mRNA level of SOD in hepatopancreas decreased, and the expression of GPx and CAT increased with the decreasing FM levels. The Toll pathway was affected by dietary FM levels, the expression of Toll2, TNFSF, MyD88, Rho and p38 in intestine were increased with the decreasing FM levels. The results indicated that at low salinity condition, fish meal level lower than 15% would inhibit the protein synthesis and harm to the health of shrimp.
研究领域:
水产养殖
·
分子生物学
·
营养学
✓ 已验证
DOI
PubMed
高影响因子
Sensor Review
·
2025
·
IF 1.4
Zhaopeng Li,Pei Li,Peisi Yin,Xin Zhao,Yangting Hu,Fei Sun,Xia Sun,Zhenhe Wang
Purpose This paper aims to develop a highly sensitive resistive gas sensor for accurately detecting ethyl formate to achieve reliable and real-time monitoring of fish meal spoilage. Design/methodology/approach Based on the one-step solvothermal reduction method, in a specific triethylene glycol solution environment and high temperature, the 3D ZnO sensing material with a jackfruit-like structure was prepared for detecting ethyl formate gas in fish meal. Findings The gas sensor based on 3D ZnO displays high sensor response (69.68–100 ppm) at 280°C and 43% RH and good sensor response (12.18–100 ppm) at 280°C and 80% RH, ultra-low detection limit of 10 ppb at 43% RH, excellent selectivity, repeatability and long-term stability. The sensing mechanism is due to the gain or loss of electrons caused by the sensor surface reaction. The unique jackfruit-like structure, abundant oxygen vacancies and large surface area may be another factor contributing to its excellent ethyl formate gas sensing performance. Originality/value The authors first developed an ethyl formate gas sensor, and the results were compared with the previously published data. The analysis showed the ZnO gas sensor demonstrated excellent sensing performance. This work highlights the potential of high response ethyl formate gas sensors to accurately evaluate fish meal quality.
研究领域:
材料科学
·
食品科学
✓ 已验证
产品关联
DOI
高影响因子
PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY
·
2022
·
IF 3.141
Yi Zhang,Jing Bai,Chenqi Wu,Yue Wang,Xin Ju,Xin Qi,Liangzhi Li,Lilian Ji,Jiaolong Fu
ε-Poly-l-lysine (ε-PL) is used as a natural food preservative which consists of l-lysine units connected. However, due to the expensive culture medium, the production cost of ε-PL remains high. In this study, cheap raw materials cassava starch (CS) and fish meal (FM) were employed by S. albulus FQC-24 for ε-PL production. In the single factor experiment, the maximum ε-PL production reached 0.97 g/L at 60 g/L CS and 15 g/L FM. The results of screening experiments by Plackett–Burman design showed that three main components affecting ε-PL production were CS, FM, and (NH4)2SO4. And the standardized effects of CS, FM, and (NH4)2SO4 were 0.13, −0.22, and −0.2, respectively. The optimum fermentation medium developed by response surface methodology for ε-PL production contained (g/L) CS, 67.56; FM, 14.70 and (NH4)2SO4, 5.41. Under the optimum conditions, the ε-PL production was achieved 1.30 g/L, with 34.02% higher than that before optimization. Moreover, ε-PL productions of batch and fed-batch fermentation in a 7-L fermentor were improved to 2.13 and 17.17 g/L respectively, which increased by 0.64 and 12.2 times compared with the shake flask culture. The results indicated that FM and CS are promising substrates for the efficient production of ε-PL.
研究领域:
生物技术
·
微生物学
·
食品科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
AQUACULTURE NUTRITION
·
2018
·Vol. 24
·期 6
·1845-1854
·
IF 2.078
Migen Wan,Peng Yin,Weiping Fang,Shiwei Xie,Shi Jun Chen,Li Xia Tian,Jin Niu
An 8-week feeding trial was conducted to evaluate the effects of replacing fishmeal by concentrated dephenolization cottonseed protein (CDCP) on growth, feed utilization, body composition, haemolymph indexes and antioxidative ability of juvenile white shrimp Litopenaeus vannamei , Boone. Six practical diets CDCP0, CDCP50, CDCP100, CDCP150, CDCP200 and CDCP250, containing a gradient of CDCP 0g/kg, 50g/kg, 100g/kg, 150g/kg, 200g/kg and 250 g/kg) to substitute (50g/kg, 90g/kg, 130g/kg, 170g/kg, 210g/kg and 250 g/kg) of fishmeal, diets were fed to four replicate groups. Amino acids were supplemented in the low fishmeal diet to obtain similar amino acid profile as the control diet. The results indicated that shrimp fed the CDCP20 and CDCP25 diet had poor growth performance and feed utilization compared with the CDCP0 group. No difference was observed in body composition among all the treatments. Total cholesterol (T-CHO), high-density lipoprotein cholesterol (HDL-C) and low-density lipoprotein cholesterol (LDL-C) concentrations in haemolymph were significantly lower in high-CDCP groups. The digestibility of protein decreased significantly with increasing CDCP levels. When shrimp fed the CDCP250 diet had the significant lower total antioxidant capacity (T-AOC) and catalase (CAT) activity than control diet. In conclusion, fishmeal can be reduced from 250g/kg to 130 g/kg by CDCP.
研究领域:
水产养殖
·
营养学
·
生物化学
✓ 已验证
DOI
高影响因子
ANIMAL FEED SCIENCE AND TECHNOLOGY
·
2024
·
IF 2.5
Xinzhou Yao,Tengfei Zhao,Xiaoyue Li,Xinchen Zhang,Xin Chen,Haoming Li,Beiping Tan,Shiwei Xie
Plant protein sources are the main alternative to fish meal (FM), but excessive substitution can induce inflammatory responses and oxidative stress in aquatic animals. In this study, the antioxidant capacity, endoplasmic reticulum stress (ERS), apoptosis and intestinal health of Litopenaeus vannamei at the different stages of feeding trial were evaluated after replacing FM with soy protein concentrate (SPC). Two diets were designed: HF diet (25% FM) and LF diet (15% FM + 10% soy protein concentrate), respectively. The samples were collected at 7d, 14d, 28d, 42d, 56d of the feeding trial. The results showed that the antioxidant capacity of the hepatopancreas of shrimp fed the LF diet gradually decreased after 28d. The glutathione peroxidase (GSH-PX) activity, superoxide dismutase (T-SOD) activity and total antioxidant capacity (T-AOC) showed a decreasing trend with the went on of the feeding trial and were lower than those of shrimp fed a HF diet. The length of microvilli and height of mucosal folds in shrimp fed the LF diet were significantly lower than those fed the HF diet after 42d ( P <0.05), and the endoplasmic reticulum and mitochondria were swollen, and the mitochondrial matrix showed irregular structure and even almost complete dissolution at 56d. The expression of hepatopancreatic endoplasmic reticulum stress (ERS)-related genes was highest at 14d, and the hepatopancreatic endoplasmic reticulum-related genes were overall higher in shrimp fed the LF diet than those fed the HF diet after 28d. The expression of apoptosis-associated genes in hepatopancreatic cells showed an overall decreasing trend. However, the relative expression of intestinal ERS and apoptosis related gene was in the opposite trend. ERS was enhanced and apoptosis was inhibited in shrimp since 7d of the feeding trial when the dietary FM level was reduced. In conclusion, the antioxidant capacity and immune response of shrimp would be reduced as the feeding trial went on, ERS and apoptosis in the intestine of shrimp fed a LF diet would be earlier to be induced compared to those fed the HF diet.
研究领域:
水产养殖学
·
营养与饲料学
·
分子生物学
✓ 已验证
DOI
高影响因子
AQUACULTURE NUTRITION
·
2018
·Vol. 24
·期 4
·1184-1197
·
IF 2.078
S. Xie,J. Niu,W. Zhou,Y. Liu,L. Tian
An 8-week feeding trial was conducted to evaluate the effect of fish-meal replacement on growth performance, antioxidative ability, immune capacity and haemolymph metabolites of Litopenaeus vannamei . A 250 g/kg fish-meal diet was used as control (Diet 1). Other two diets contained 100 g/kg fishmeal, Diet 2 was supplemented with three indispensable amino acids, and Diet 3 was supplemented with microbalance components and phytase. Weight gain was lower in shrimp fed the Diet 2 than shrimp fed the Diet 1. Feed efficiency, protein efficiency ratio and survival were higher in shrimp fed the Diet 1 than other groups. Glutathione and nitric oxide contents in haemolymph were decreased in shrimp fed the Diet 2, and nitric oxide synthetase, phenoloxidase, acid phosphatase and myeloperoxidase activities showed the similar tendency. In hepatopancreas, antioxidative ability and immune capacity also lower in shrimp fed the Diet 2. In haemolymph, 21 differential metabolites were identified, and 13 metabolism pathways were affected by different diets. Generally, the fishmeal inclusion level could be reduced from 250 g/kg to 100 g/kg without affecting the growth performance of L. vannamei , and meanwhile, the protein utilization efficiency and antioxidative ability of shrimp fed the low fishmeal diets should be more focused on in future studies.
研究领域:
水产养殖
·
营养学
·
海洋生物学
✓ 已验证
产品关联
DOI
高影响因子
Aquaculture Reports
·
2023
·
IF 3.7
Menglin Shi,Xinzhou Yao,Kangyuan Qu,Yucheng Liu,Beiping Tan,Shiwei Xie
This experiment investigated the impact of taurine supplementation in low fishmeal diets on growth, antioxidant capacity, immunity and intestinal health of Litopenaeus vannamei . In this experiment, five diets were formulated, of which the high fishmeal diet contained 25% fishmeal (HF) and the remaining four diets were a mixture of soybean protein concentrate and Clostridium autoethanogenum protein (CAP) replacing 15% fishmeal and supplemented with 0%, 0.2%, 0.4% and 0.6% taurine (LF, T1, T2, T3). A total of 800 healthy shrimp (0.32 ± 0.00 g) were randomly allocated into 5 groups, with each group consisting of 4 replicates and 40 shrimp per replicate. The shrimp were fed four times a day for 51 days. The results showed that there was no significant difference in weight gain rate and survival ( P ＞0.05). Dietary taurine supplementation significantly improved the antioxidant capacity of shrimp. Compared with LF group, the mRNA expression of alf (anti-lipopolysaccharide factor) and cru (crustins) in HF group were significantly up-regulated ( P ＜0.05). Compared with the HF and LF groups, the mRNA expression of pen3 (penaeidins 3) in T1, T2 and T3 groups was significantly up-regulated, and the mRNA expression of dorsal in T2 group was significantly up-regulated ( P ＜0.05). The mRNA expression of bcl-2 (B-cell lymphoma-2) in T2 group was significantly up-regulated than that in other groups ( P ＜0.05). The mRNA expression of cyt-c (cytochrome c) in LF group was significantly up-regulated than that in other groups ( P ＜0.05). Compared with LF group, the mRNA expression of tnf-a (tumor necrosis factor-alpha) in HF, T2 and T3 groups was significantly down-regulated ( P ＜0.05). The mRNA expression of caspase8 in HF and T2 group was significantly down-regulated than that in other groups ( P ＜0.05). Compared with LF group, the mRNA expression of caspase3 in T1 groups was significantly down-regulated ( P ＜0.05). Intestinal H&E stained sections showed that dietary taurine supplementation improved intestinal mucosal folds height, width and muscle layer thickness. In general, dietary taurine supplementation can improve the antioxidant capacity, immunity and intestinal health of Litopenaeus vannamei , and the optimal supplemental level is 0.4%.
研究领域:
水产养殖
·
营养学
·
免疫学
·
海洋生物学
✓ 已验证
DOI
高影响因子
AQUACULTURE
·
2015
·
IF 1.878
Shi-Wei Xie,Li-Xia Tian,Yu-Ming Li,Weiwen Zhou,Shuai-Lin Zeng,Hui-Jun Yang,Yong-Jian Liu
An 8-week feeding trial was conducted to evaluate the effect of proline on anti-oxidative capacity, immune response and stress tolerance of juvenile Pacific white shrimp , Litopenaeus vannamei . Six practical low fishmeal diets (15% fishmeal) were formulated to contain graded levels (2.02, 2.14, 2.27, 2.38, 2.49, 2.6%) of l -proline. Each diet was randomly assigned to triplicate groups of 30 shrimps (approximately 0.41 g) and the shrimps were fed 4 times a day to apparent satiation. Growth performance was not affected by proline levels (P > 0.05). But higher levels of proline increased hydroxyproline content in the whole body (P = 0.004). And survival after 60 h acute NH 3 stress increased with the increasing dietary proline levels (P = 0.015). High levels of proline also increased the proline oxidase (POX), nitric oxide (NO), total antioxidant capacity (T-AOC) and phenoloxidase (PO) activities in hemolymph or hepatopancreas after the 60 h NH 3 stress compared to basic group which had lowest proline level. Broken-line analysis showed that the optimal dietary proline requirement of L. vannamei was 2.29%, 2.32% and 2.34% based on PO, POX and 60 h survival after NH 3 stress. These results clearly indicated that 2.29–2.34% of proline in the low fish meal diet could improve anti-oxidative capacity, immune response, NH 3 stress tolerance of L. vannamei , and proline may be a conditionally essential AA for L. vannamei . Statement of relevance This study indicates that proline may be a conditional amino acid for white shrimp under the environment stress, especially when fed with low fish meal diet. The results will contribute to the low fish meal diet study of the white shrimp.
研究领域:
水产养殖
·
营养学
·
海洋生物学
✓ 已验证
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Zhaopeng Li,Pei Li,Jinfu Zhang,Helei Yang,Zhiguo Zhu,Hua Zhou,Xia Sun,Zhenhe Wang
This study reports on the development of a NiO quantum dot-modified ZnO gas sensor, which is designed for detecting ethyl formate, a crucial indicator of fish meal spoilage. The results indicated that NiO quantum dots were uniformly dispersed on the surface of ZnO, leading to the formation of a P N heterojunction that enhanced the gas sensing properties. Among the sensors tested, the S2 sensor (with a NiO doping of 0.1 mmol) exhibited the highest response value of 15.75 when exposed to 100 ppm of ethyl formate at 260 °C, additionally presented out excellent selectivity, linear response behavior, and long-term stability. The superior performance of the S2 sensor can be attributed to its unique jackfruit-like structure and the formation of P N junctions. These features increase the number of active sites and modulate the electronic properties of the material. The S2 sensor has been implemented for the assessment of fish meal freshness. This study emphasizes the great potential of this sensor for the real-time monitoring of fish meal freshness, offering significant applications in the field of food and feed safety.
研究领域:
材料科学
·
纳米技术
·
食品安全
·
化学传感
·
环境监测
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）