MOLECULES
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2024
·Vol. 29
·期 1
·263
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IF 4.2
Shuji Liu,Yue Zhang,Yihui Chen,Yongchang Su,Bei Chen,Yin Wang,Min Xu,Kun Qiao,Shuigen Li,Zhiyu Liu
Protamine is a cationic peptide derived from fish sperm and has several important functional properties: antibacterial properties, acting as a carrier for injectable insulin and as a heparin antagonist, combatting fatigue, etc. Thus, it has been widely used in medicinal applications and food products. CulturedTakifugu flavidusis a type of pufferfish with a delicious taste that is popular in China, and its production is increasing significantly. Therefore, protamine was extracted via acid extraction from the sperm ofTakifugu flavidusand further isolated and purified via sephadex gel chromatography, ion exchange chromatography, and desalination chromatography. Furthermore, the physicochemical properties of protamine were investigated. The results showed that the sperm of the culturedT. flaviduswere non-toxic, and the extracted and purified protamine had high contents of arginine (36.90%) and lysine (27.02%), respectively. The secondary structure of protamine was mainly β-folded and irregularly curled. Additionally, protamine exhibited high thermal stability with a denaturation temperature of 176 °C. This study would provide a theoretical basis for the structural analysis, bioactivity, and resource development of pufferfish protamine and help to promote the development of the pufferfish industry.
研究领域:
生物化学
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医药学
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食品科学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
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2015
·
IF 3.368
Linlin Wang,Jing Song,Shaopu Liu,Chenxia Hao,Nianxi Kuang,Youqiu He
Even though various strategies have reported for DNA detection, development of a simple, time-saving and specific fluorescent sensing platform still remains a desired goal. In this work, a quantum dots (QDs) based fluorescent reversible “off–on” mode was developed for sensitively recognition of herring sperm DNA (hsDNA). Firstly, in the “turn off” stage, the fluorescence of glutathione (GSH) capped CdTe QDs could be effectively quenched by ytterbium ion (Yb 3+ ) was due to the occurrence of the electron transfer between Yb 3+ and the photoexcited QDs. And then, in the following “turn on” stage, with the effective binding reaction of Yb 3+ to hsDNA, the fluorescence intensity of GSH-capped CdTe QDs enhanced. Under the optimal conditions, the linear range of fluorescence versus the concentration of hsDNA was 0.010–12 μg/mL, and the detection limit was 3.033 ng/mL. In addition, the reaction mechanism among GSH-capped CdTe QDs, Yb 3+ and hsDNA were investigated by fluorescence spectroscopy, UV–vis spectrophotometry, fluorescence lifetime measurement and viscosity measurements. This analytical fluorescent reversible “off–on” pattern offered a way with good sensitivity and selectivity for the detection of hsDNA.
研究领域:
生物化学
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分析化学
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产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
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2014
·
IF 3.84
Yizhong Shen,Shaopu Liu,Jidong Yang,Linlin Wang,Xuanping Tan,Youqiu He
A novel and sensitive turn-on fluorescent biosensor for herring sperm DNA (hsDNA) detecting was designed based on hsDNA-induced fluorescence enhancement of Sm 3+ -modulated glutathione (GSH)-capped CdTe quantum dots (QDs). The fluorescent biosensor was based on the following two properties. First, the high fluorescence of GSH-capped CdTe QDs could be effectively quenched by Sm 3+ due to the binding of Sm 3+ to the GSH on the surface of the QDs and the electron transfer from the photoexcited GSH-capped CdTe QDs to Sm 3+ . Second, the high affinity of hsDNA to Sm 3+ enabled Sm 3+ to be dissociated from the surface of GSH-capped CdTe QDs to form more stable complex with hsDNA in aqueous solution, thereby enhancing the fluorescence of Sm 3+ -modulated GSH-capped CdTe QDs. Experimental results showed that the enhanced fluorescence intensity of Sm 3+ -modulated GSH-capped CdTe QDs was directly proportional to the concentration of hsDNA in the range from 0.012 to 14.0 μg mL −1 with a good correlation coefficient of 0.9977, and the detection limit (3 σ / S ) was 3.61 ng mL −1 . To further investigate perfect analysis performance, the developed biosensor was applied to the determination of hsDNA in synthetic samples and human fresh serum samples with satisfactory results.
研究领域:
生物化学
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分析化学
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DOI
高影响因子
International Journal of Electrochemical Science
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2023
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IF 1.5
Li Sun,Xueliang Wang,Na Zou,Hongying Li,Zhangyu Yu
The interaction of formononetin (FN) with herring sperm DNA has been studied by electrochemical methods, UV-Vis spectroscopy and fluorescence. On a poly L-cysteine film modified electrode (PLC/GCE), FN showed a couple of well-defined voltammetric peaks in 0.1 M acetate buffer of pH 5.3. As adding the herring sperm DNA into above solution, the peak current of FN decreased and no new peaks appeared. Based on the electrochemical results, the binding constant and binding ratio between FN and herring sperm DNA were determined to be 1.98×10 4 M -3 and 1:3, respectively. The UV-Vis spectroscopy indicated that the maximum absorption of FN had obviously hyperchromic effect and red shift after the interaction of DNA and FN. The interaction of DNA and FN can also make the fluorescence intensity of FN enhancement. The electrochemical and spectroscopic results indicated that the interaction of FN to DNA was an intercalative mode.
研究领域:
生物化学
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药理学
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DOI
高影响因子
Journal of Engineered Fibers and Fabrics
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2020
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IF 0.814
Qiulan Luo,Pu Gao,Jie Zhou,Jian Zhang,Wen Wu,Jianda Cao,Narendra Reddy,Hui Ma
Textiles are one of the basic needs of humankind. Although textiles satisfy many human needs, they are implicated in the loss of life and property since many fibers and fabrics are inflammable. Many chemical finishes are applied to improve the flame resistance of textile materials. Unfortunately, most of the flame retardants are synthesized chemically, need large quantities to be used, cause adverse impact on the textiles and also expensive. In this research, a novel approach to using DNA from herring sperm as flame retardant for cotton was explored. Surface of the textiles was modified with citric acid to increase free carboxyl content and hence absorption of DNA. It was observed that DNA was absorbed into the cotton fabrics using chemical adsorption according to the quasi-second-order-kinetic model. Flammability of the cotton before and after the addition of DNA was checked. It was observed the length of fabrics damaged due to flames was only 29 mm compared to the original length of 350 mm. Such a high increase in flame resistance has not been reported for natural materials. In addition, the chemical reaction between DNA and cotton fabric can improve the flame retardant–washing resistance of the finished cotton fabric. In this article, the strategy of using biological macromolecules to flame-retardant fabrics can provide research basis for the design and development of efficient and environmentally friendly flame retardants.
研究领域:
纺织科学
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材料科学
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化学工程
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环境科学
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产品关联
DOI
高影响因子
RSC Advances
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2021
·Vol. 11
·期 6
·3324-3332
·
IF 3.361
Jia Xu,Dan Chen,Yuan Yang,Hongjian Gong,Wenqi Gao,Han Xiao
A simple and efficient approach for the rapid extraction of genomic DNA from blood using various amino-modified magnetic nanoparticles (AMNPs) has been described. The salmon sperm DNA was isolated from aqueous solution based on electrostatic interaction between the positively charged amino-groups of AMNPs and the negatively charged phosphate groups of the DNA. The results of ultraviolet-visible (UV-Vis) spectrometry showed that increasing number of amino groups on the AMNPs surface resulted in an improvement in DNA adsorption efficiency. Several variables including the extraction pH, adsorption time, ionic strength and quantity of AMNPs were optimized to achieve the best extraction efficiency with the proposed method. Acceptable adsorption efficiency of 92% and recovery of 91% were achieved using multi-amino modified MNPs (mAMNPs) with an extraction time of 10 min and an overall processing time of 30 min. The mAMNPs enabled genomic DNA capture from human whole blood, and the resulting mAMNP/DNA complexes could be directly used as templates for PCR amplification without the need for complex and time-consuming DNA elution and purification steps. Our results imply that this method can be used as an effective strategy for genomic DNA extraction and may be extended to other types of biological samples.
研究领域:
分子生物学
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纳米技术
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生物技术
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产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
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2020
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IF 3.594
Xin Zhao,Hao Guo,Rui Xue,Mingyue Wang,Qixia Guan,Tian Fan,Wenhu Yang,Wu Yang
Alteration in the concentrations of guanine (G) and adenine (A) leads to various abnormalities in the metabolic process which causes various diseases. Therefore, it still remains a challenge to establish an accurate, simple and accurate method for simultaneous detection of guanine and adenine. In this paper, using covalent organic frameworks (COFs) as a carrier and the amino functionalized reduced graphene oxide as charge transfer accelerator, layer molybdenum disulfide (MoS 2 ) nanosheets are deposited on glassy carbon electrode (GCE) by simple physical stacking to fabricate a dual-signal amplification biosensor for simultaneous detection of adenine and guanine. Under optimized conditions, the electrochemical behaviors of guanine and adenine on the COFS/NH 2 -rG/MoS 2 modified electrode were studied by cyclic voltammetry and differential pulse voltammetry. The sensor performs well in determining G and A at the same time with wide linear ranges of 0.5–150.0 μM and 1.0–280 μM and low detection limits of 0.51 μM(S/N = 3) and 0.44 μM(S/N = 3), respectively. The sensor was successfully used to assay G and A in thermally denatured herring sperm DNA, and the G/A ratio was calculated to be 0.79. The biosensor has the advantages of simplicity, speed, high sensitivity, good reproducibility, and long-term stability.
研究领域:
电化学
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生物传感
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纳米材料
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分析化学
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生物化学
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DOI
高影响因子
Nanoscale
·
2013
·Vol. 5
·期 22
·10982-10988
·
IF 6.233
Lijian Chen,Kaifang Sun,Peipei Li,Xianzhong Fan,Jianchao Sun,Shiyun Ai
LDH nanosheets were obtained via continuous impaction and exfoliation by herring sperm DNA molecules using a constant vibration method. DNA–LDH nanohybrids were composed by electrostatic forces and they exhibited DNA-enhanced peroxidase-like activity. The morphology and structure of DNA–LDH nanohybrids were analyzed by transmission electron microscopy (TEM), selected-area electron diffraction (SAED), X-ray diffraction (XRD), and atomic force microscopy (AFM) characterization. On the basis of the high catalytic activity of DNA/CuAl–LDH nanosheets, a rapid, sensitive, and convenient approach was developed for colorimetric detection of H2O2 and blood glucose. This method can be potentially applied in medical diagnostics and biotechnology fields.
研究领域:
纳米技术
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生物传感器
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催化
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材料科学
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医学诊断
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）