GLYCOCONJUGATE JOURNAL
·
2017
·Vol. 34
·期 5
·625-632
·
IF 2.186
Song Shuang,Yu Qi,Zhang Bao,Ai Chunqing,Sun Yujiao,Fu Yinghuan,Zhao Meiyu,Wen Chengrong
Fish intestines and livers are usually considered as delicious and nutritious food in China. Acidic polysaccharides are important nutrients in these food of animal origin, but there is currently little information regarding their quantitative distributions. The present study demonstrated a method to quantify acidic polysaccharides simultaneously by analyzing their disaccharides produced from the acid hydrolysis using high-performance liquid chromatography (HPLC) coupled with triple quadrupole mass spectrometry. The recoveries for these acidic polysaccharides were all 97%–115% with relative standard deviation of 3.0%–9.0%. All of the acidic polysaccharides had good linearities. Then this method was applied to determine the composition of acidic polysaccharides in 5 edible fish livers and intestines. Besides well-known glycosaminoglycans (GAGs) including hyaluronic acid (HA), Chondroitin sulfate (CS), dermatan sulfate (DS) and heparin (HP), 4 novel acidic polysaccharides including 2 GAGs and 2 non-GAGs comprised of hexose-hexuronic acid repeating units were also found. CS and HP were the major acidic polysaccharides components in fish intestines and livers, respectively. The absolute amounts of acidic polysaccharides differed greatly in these fish tissues, but their proportions showed similarity in the same type of tissues. The present study demonstrated an effective method for acidic polysaccharides quantification, and revealed acidic polysaccharides compositions of edible fish livers and intestines.
研究领域:
食品科学
·
分析化学
·
营养学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FISH & SHELLFISH IMMUNOLOGY
·
2015
·
IF 2.674
Tao Wang,Xiaohua Long,Zhaopu Liu,Yongzhou Cheng,Shaohua Yan
Copper nanoparticles (Cu-NPs) are widely used in various industrial and commercial applications, but little is known about their potential hazard in the intestines of marine teleosts. In this study we investigated the effects of Cu-NPs and soluble Cu in the intestines of juvenile Epinephelus coioides . The fish were exposed in triplicate to control, 20 or 100 μg Cu L −1 as either copper sulphate (CuSO 4 ) or Cu-NPs for 25 days. With an increase in Cu-NPs or CuSO 4 dose, the concentration of malonaldehyde in the intestines significantly increased, whereas the activities of total superoxide dismutase and catalase as well as glutathione concentration decreased compared to the control. Statistical analysis of an apoptosis index of intestinal cells showed that general dose-dependent apoptosis was induced by Cu-NPs or CuSO 4 , with Cu-NPs inducing the significantly higher apoptosis index than CuSO 4 . Caspase-3 and caspase-9 activities were increased with an increase in Cu-NPs or CuSO 4 dose, more so in the Cu-NPs than CuSO 4 treatment. With an increase in Cu-NPs or CuSO 4 dose, succinate dehydrogenase and Na + -K + -ATPase activity and cytochrome c concentration in mitochondria decreased, accompanied by increased cytochrome c concentration in the cytosol. Concentration of heat shock proteins 70 and 90 in the intestines and expression of corresponding genes were enhanced with an increase in the Cu-NPs or CuSO 4 dose, but the concentrations and expressions of immunoglobulin M and lysozyme decreased (more in the Cu-NPs than CuSO 4 treatment) compared to the control. Expression of interleukin-1beta and tumor necrosis factor-alpha showed a dose-dependent increase with the increased Cu-NPs or CuSO 4 dose, with the highest expression found in the Cu-NPs treatment. In conclusion, Cu-NPs had similar toxic effects as CuSO 4 in the intestines of juvenile E. coioides , but toxicity of Cu-NPs was more severe than that of CuSO 4 .
研究领域:
环境毒理学
·
水产养殖学
·
分子生物学
✓ 已验证
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2025
·
IF 13.2
Yuan-shuai Fu,Yue Lin,Wen-jun Zhong,Xing-yu Wang,Xi-ke Li,Yun-ao Li,Sheng-bo Ye,Wen-juan Li,Yong-heng Zhu
Bacterial enteritis is a prevalent disease in Micropterus salmoides . Conventional drug delivery approaches are typically characterized by low efficiency and induce drug resistance. The present study synthesized mesoporous hollow silica nanoparticles (HMSS) and used physical adsorption to load enrofloxacin (ENR) and astaxanthin (ASX) onto the nanoparticles, achieving adsorption efficiencies of 77.39 % and 32.77 %, respectively. To establish the drug-targeted delivery system, ASX@HMSS was functionalized with cell-penetrating peptide (CPP) and ENR@HMSS was encapsulated with hydroxypropyl methylcellulose phthalate (HP55). Positive sustained-release effects were demonstrated by in vitro release studies, which demonstrated that the cumulative release rate of ASX@HMSS-CPP varied from 29.58 to 38.47 % at pH 6.5, whereas the cumulative release rate of ENR@HMSS-HP55 ranged from 70.19 to 88.25 %. The drug-targeted delivery nanoparticles extended the residence time of the drug in the intestines, according to in vivo pharmacokinetic investigations, and the overall metabolic profile was positive. Additionally, these drug-targeted delivery nanoparticles reduced intestinal inflammation and tissue damage, enhanced antioxidant and anti-inflammatory indicators, and improved the survival rate of Micropterus salmoides infected with enteritis. Following the administration of the drug-targeted delivery nanoparticles, the abundance of Aeromonas was significantly regulated, aiding gut microbial homeostasis. By controlling the PI3K-Akt, NF-κB, MAPK, and intestinal IgA immune network signaling pathways, the drug-targeted delivery nanoparticles also reduced intestinal inflammation. These findings demonstrate that the nanoparticle-based drug-targeted delivery system has potential applications in the prevention and treatment of diseases in aquatic animals.
研究领域:
水产动物医学
·
纳米药物递送
·
微生物学
✓ 已验证
产品关联
DOI
高影响因子
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2020
·Vol. 27
·期 12
·13409-13416
·
IF 3.056
Wang Xiaofang,Hui Hongjie,Yu An,Jiang Ziyang,Yu Hongyan,Zou Luyi,Teng Yue
Triclosan (TCS), a broad-spectrum antibacterial agent, exhibits a high exposure in the environment. However, the residual TCS in the environment poses a potential risk to human health. In this study, spectroscopic methods, molecular docking and animal experiment were conducted to completely understand the interaction between trypsin and TCS. The formation of the TCS-trypsin complex was spontaneously achieved through hydrogen bonds and Van der Waals forces with a binding constant ( K a ) between 10 3 and 10 4 L mol −1 . In addition, the trypsin activity in fish intestine was inhibited by TCS exposure, revealing the potentially negative effects of TCS on metabolism. The results might be explained by changes in the conformation of the trypsin, inducing the content of unordered coil increasing significantly (from 36.2% to over 80%). This work provides useful information for assessing the toxicity of TCS at the molecular level.
研究领域:
环境科学
·
生物化学
·
毒理学
·
分子生物学
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）