ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
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2020
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IF 4.872
Qingfeng Wang,Zhonggen Li,Xinbing Feng,Xinyu Li,Dan Wang,Guangyi Sun,Huihui Peng
Consumption of mercury (Hg) contaminated vegetable is one important pathway of Hg expose to humans. In this study, Hg contents in a popular vegetable, Houttuynia cordata Thunb. ( HCT ), and its growing soils in Kaiyang county in Guizhou province of southwest China were investigated. Health risk related to Hg exposure through consumption of this vegetable was evaluated for the first time. Hg contents in HCT were found to be much higher in three towns in western Kaiyang county (42.3 ± 48.2 μg/kg, FW), where former Hg mines located, than that in other towns (7.6 ± 5.0 μg/kg, FW). Hg contents in HCT were also higher than in the other five vegetables (Chinese cabbage, Lettuce, Tomato, Carrot and White radish). Consumption of HCT may account for 37.4–61.1% of total vegetable Hg intake of local people in Kaiyang county. Hg concentration in HCT positively correlated with that in soil (r 2 = 0.311, p < 0.01), especially, the labile Hg species (F C1 , r 2 = 0.796, p < 0.01) and the elemental Hg that is bound to the crystalline oxides (F C3 , r 2 = 0.711, p < 0.01), and negatively correlated with Hg that is bound to humic and fulvic complexes (F C2 , - 0.304). Estimated daily intake (EDI) and target hazard quotient (THQ) results shown that Hg expose risk is much higher for children than adults, likely due to their different eating habits and the amount of snack intake.
研究领域:
环境科学
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公共卫生
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产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Xinyue Lin,Luxi Zhou,Lingfang Chen,Qiyue Chen,Zhuming Jin,Peixi Zhu,Ni Yang
Houttuynia cordata Thunb. is a perennial herb with a distinct “fishy” flavour, commonly used in Asian cuisine and traditional medicine. To overcome the compound identification challenges in the composite formula of its mixture, a comprehensive two-dimensional ultra-high-performance liquid chromatography quadrupole time-of-flight mass spectrometry (2D UHPLC-QTOF-MS) method was developed and identified 293 chemical compounds. Given the herb’s strong anti-inflammatory properties, network pharmacology was employed to predict potential targets, revealing TNF, AKT1, MMP9, and PTGS2 as key theoretical targets for its anti-inflammatory effects. Molecular docking showed strong binding energies between these targets and the top 10 compounds (8,13-dihydroxy-9,11-octadecadienoic acid, neobaicalein, chrysin, apigenin, genistein, chrysoeriol, norwogonin, cirsiliol, luteolin and deoxyandrographolide), with genistein exhibiting the highest affinity for AKT1 (−10.4 kcal/mol). This innovative approach, combining advanced 2D UHPLC-QTOF-MS with network pharmacology, offers great potential for screening active components in herbs and their complex mixtures.
研究领域:
中药学
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分析化学
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药理学
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DOI
高影响因子
JOURNAL OF APPLIED TOXICOLOGY
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2014
·Vol. 35
·期 3
·295-301
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IF 3.174
Rui Yang,Qiao-Cong Lao,Hang-Ping Yu,Yong Zhang,Hong-Cui Liu,Lin Luan,Hui-Min Sun,Chun-Qi Li
A number of recent reports suspected that Tween-80 in injectable medicines, including traditional Chinese medicine injections could cause life-threatening anaphylactoid reaction, but no sound conclusion was drawn. A drug-induced anaphylactoid reaction is hard to be assayed in vitro and in conventional animal models. In this study, we developed a microplate-based quantitative in vivo zebrafish assay for assessing anaphylactoid reaction and live whole zebrafish mast cell tryptase activity was quantitatively measured at a wavelength of 405 nm using N -benzoyl- dl -arginine p-nitroanilide as a substrate. We assessed 10 batches of Tween-80 solutions from various national and international suppliers and three Tween-80 impurities (ethylene glycol, 2-chloroethanol and hydrogen peroxide) in this model and found that three batches of Tween-80 (nos 2, 20080709 and 20080616) and one Tween-80 impurity, hydrogen peroxide (H 2 O 2 ), induced anaphylactoid reactions in zebrafish. Furthermore, we found that H 2 O 2 residue and peroxide value were much higher in Tween-80 samples 2, 20080709 and 20080616. These findings suggest that H 2 O 2 residue in combination with oxidized fatty acid residues (measured as peroxide value) or more likely the oxidized fatty acid residues in Tween-80 samples, but not Tween-80 itself, may induce anaphylactoid reaction. High-throughput zebrafish tryptase assay developed in this report could be used for assessing safety of Tween-80-containing injectable medicines and potentially for screening novel mast cell-modulating drugs. Copyright © 2014 John Wiley & Sons, Ltd.
研究领域:
药理学
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毒理学
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免疫学
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DOI
PubMed
高影响因子
FISH & SHELLFISH IMMUNOLOGY
·
2025
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IF 3.9
Mengmeng Li,Mingyang Xue,Yong Zhou,Wenzhi Liu,Yan Meng,Chen Xu,Yiqun Li,Nan Jiang,Yuding Fan
Grass carp reovirus (GCRV), which causes hemorrhagic disease in grass carp ( Ctenopharyngodon idella ), poses a significant threat to grass carp aquaculture. To develop effective treatments against GCRV infection, this study evaluated the antiviral efficacy of natural compounds derived from Chinese herbs on GCRV-infected grass carp kidney cells (CIK). Among the various compounds examined, in CIK cells, Andrographolide (Andro) at 25 mg/L exhibited the highest inhibition rates against GCRV-I and GCRV-II infections (83.27% and 87.40%, respectively). In vitro experiments showed that Andro significantly reduced the cytopathic effect, alleviated the apoptosis of GCRV-I-infected cells, inhibited the increase of mitochondrial membrane potential, and blocked CIK cell adsorption of GCRV-I and GCRV-II. Addition time studies revealed that Andro chiefly wielded its antiviral impact in the initial phase of viral replication (0–4 h). In vivo studies demonstrated that in both prevention and treatment experiments, 50 mg/kg Andro in the feed increased the survival rates of grass carp infected with GCRV-Ⅱ by 46.66% and 40%, respectively. Moreover, this compound significantly reduced the viral loads and upregulated antiviral gene expression in various organs, indicating its potential for immune modulation. In conclusion, Andro displayed significant antiviral properties that are effective to both prevent and treat GCRV infections, thus holding promise as a novel therapeutic agent for this virus.
研究领域:
水产养殖学
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病毒学
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药理学
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DOI
PubMed
高影响因子
MOLECULES
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2023
·Vol. 28
·期 13
·5172
·
IF 4.6
Yue Sun,Jia-Xin Feng,Zhong-Bao Wei,Hui Sun,Li Li,Jun-Yi Zhu,Guang-Qing Xia,Hao Zang
Euphorbia fischerianahas a long-standing history of use in traditional medicine for the treatment of tuberculosis diseases. However, the plant’s therapeutic potential extends beyond this specific ailment. The present study aimed to investigate the antioxidant properties ofEuphorbia fischerianaand lay the groundwork for further research on its potential therapeutic applications. Phytochemical tests were performed on the plant, and 11 types of phytochemicals were identified. Ultraviolet–visible spectrophotometry was used to evaluate the active components and antioxidant properties of eight different solvent extracts, ultimately selecting acetone extract for further research. UHPLC-ESI-Q-TOF-MS identified 43 compounds in the acetone extract, and chemical calculations were used to isolate those with high content and antioxidant activity. Three stability experiments confirmed the extract’s stability, while cell viability and oral acute toxicity studies demonstrated its relatively low toxicity. In rats, the acetone extract showed significant protective effects against D-galactosamine-induced liver damage through histopathological examination and biochemical analysis. These results suggest thatEuphorbia fischeriana’s acetone extract has potential in treating diseases related to oxidative imbalances. Therefore, this study highlights the plant’s potential therapeutic applications while providing insight into its antioxidant properties.Keywords:Euphorbia fischeriana;phytochemical analysis;antioxidant activity;theoretical calculation
研究领域:
药理学
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生物化学
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药物化学
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DOI
PubMed
高影响因子
MOLECULES
·
2021
·Vol. 26
·期 8
·2327
·
IF 4.412
Yuanyuan Liu,Yanfang Yang,Bangyuan Wang,Renyun Wang,Jianmei Pang,Yu Jiang,Yuling Liu
Houttuynia essential oil (HEO) has excellent antiviral, anti-inflammatory, and other pharmacological effects, but the lack of effective analytical methods to quantify HEO in plasma has hindered its better clinical monitoring. Houttuynine (Hou) is one of the main active ingredients and quality control substances of HEO, so the pharmacokinetic study of HEO could be conducted by determining Hou blood concentration. Hou is active and not stable in plasma, which makes its blood concentration difficult to measure. In this work, a novel liquid chromatography tandem mass spectrometry (LC-MS/MS) method for Hou determination in rat blood was established that involves Hou being derivatized with 2, 4-dinitrophenylhydrazine to form a stable compound to prevent degradation. Herein,p-Tolualdehyde-2,4-dinitrophenylphenylhydrazone was selected as an internal standard substance and the LC-MS/MS method was evaluated for selectivity, precision, accuracy, calibration limit, matrix effect, recovery, and stability. Good linearity (r2= 0.998) was reached in the range of 2–2000 ng/mL, and the lower limit of quantification of Hou was determined to be 2 ng/mL. The mean intra-assay accuracy ranged from 77.7% to 115.6%, whereas the intra-assay precision (relative standard deviation, RSD) was below 11.42%. The matrix effect value for Hou in rat plasma was greater than 75%, and for the internal standard (IS) it was 104.56% ± 3.62%. The extraction recovery of Hou were no less than 90%, and for the IS it was 96.50% ± 4.68%. Our method is sensitive and reliable and has been successfully applied to the pharmacokinetic analysis of Hou in rats given HEO via gavage and injection.Keywords:Houttuynia cordataThunb.;houttuynine;houttuynia essential oil;LC-MS/MS;pharmacokinetic study
研究领域:
药理学
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分析化学
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药剂学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
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2015
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IF 0.606
Rong Zhang,Lei Wang,Shanjing Liu,Gong-Jun Yang
A reliable and sensitive method of reversed-phase high-performance liquid chromatography-electrochemical detection (HPLC-ECD) was established for the simultaneous determination of five electrochemical active compounds (chlorogenic acid, rutin, hyperoside, quercitrin, and quercetin) in Houttuynia cordata Thunb. Chromatographic separation was carried out on a reversed-phase C18 column and a mobile phase comprised of 0.04 mol/L phosphate buffer (pH 4.5) and acetonitrile with gradient elution by amperometric detection with a glassy-carbon electrode at the working potential of +0.80 V. The method was validated for linearity, accuracy, precision, and limit of quantitation and detection. Under the optimized conditions, linear regression analysis for the calibration curve showed a good linear relationship with a regression coefficient beyond 0.999 between peak area and their concentrations in the linear range of 50.0 ng/mL–10.0 µg/mL for chlorogenic acid and rutin, 30.0 ng/mL–10.0 µg/mL for hyperoside, quercitrin, and quercetin, respectively. The limits of detection were found to be 5.0–20.0 ng/mL. The proposed method could be applied to the analysis of five compounds present in Houttuynia cordata Thunb. with satisfactory results.
研究领域:
分析化学
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药学
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植物化学
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DOI
高影响因子
MOLECULES
·
2025
·Vol. 30
·期 18
·3668
·
IF 4.6
Min Ye,Lifen Meng
This study used Houttuynia cordata as the precursor to prepare high fluorescence quantum yield carbon quantum dots (Hc-CQDs) by a simple hydrothermal method. The surface of the Hc-CQDs contained abundant functional groups, such as carboxyl, hydroxyl, and amino groups, which indicated the Hc-CQDs had good water solubility. On the basis of the excellent fluorescence characteristics of Hc-CQDs, a sensor was constructed to achieve high selectivity detection of Cr3+, and the detection limit of the Hc-CQDs was 49 μg/L. The sensor also exhibited strong anti-interference ability and excellent reproducibility, which was used for the determination of Cr3+in environmental water samples, and its spiked recovery rate reached over 90%. Therefore, the Hc-CQDs had potential application in the analysis.
研究领域:
纳米材料
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分析化学
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环境科学
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荧光传感器
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产品关联
DOI
PubMed
高影响因子
BIOFOULING
·
2020
·
IF 2.351
Juanjuan Liu,Qianqian Li,Changzhong Wang,Jing Shao,Tianming Wang,Daqiang Wu,Kelong Ma,Guiming Yan,Dengke Yin
Traditional herbal monomers (THMs) are widely distributed in many traditional Chinese formulas (TCFs) and decoctions (TCDs) and are frequently used for the prevention and treatment of fungal infections. The antifungal activities of five common THMs, including sodium houttuyfonate (SH), berberine (BER), palmatine (PAL), jatrorrhizine (JAT) and cinnamaldehyde (CIN), and their potential for inducing cell wall remodeling (CWR), were evaluated against Candida albicans SC5314 and Candida auris 12372. SH/CIN plus BER/PAL/JAT showed synergistic antifungal activity against both Candida isolates. Furthermore, SH-associated combinations (SH plus BER/PAL/JAT) induced stronger exposure of β-glucan and chitin than their counterparts, while CIN triggered more marked exposure compared with CIN-associated combinations (CIN plus BER/PAL/JAT). Collectively, this study demonstrated the anti-Candida effect and the CWR induction potential of the five THMs and their associated combinations, providing a possibility of their in vivo application against fungal-associated infections.
研究领域:
药理学
·
微生物学
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中医药
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产品关联
DOI
PubMed
高影响因子
MICROBIAL PATHOGENESIS
·
2023
·
IF 3.8
Shun Wang,Xiaodi Jin,Hao Chen,Mingdong Han,Jiaxin Bao,Dong Niu,Yikang Wang,Rui Li,Zhiyong Wu,Jichang Li
Chronic respiratory disease (CRD) caused by Mycoplasma gallisepticum (MG) in chickens leads to enormous economic damage to the poultry industry yearly. The active components and mechanism of action of the traditional herbal remedy Ephedra houttuynia powder (EHP), which had been approved for clinical treatment against MG infection in China, remain unknown. In this study, the active components of EHP against MG were screened using a network pharmacological method, additionally, we studied the mechanism of action of the screened results (quercetin (QUE)). The findings demonstrated that QUE was an essential element of EHP against MG infection, effectively attenuating MG-induced oxidative stress and activation of the TLR2/MyD88/NF-κB pathway. Following QUE therapy, IL-1, IL-6, and TNF-α content and expression were downregulated, whereas IL-4 and IL-10 expression were upregulated, eventually suppressing the inflammatory response both in vitro and in vivo . Together, this study presents a strong rationale for using QUE as a therapeutic strategy to inhibit MG infection-induced inflammatory damage and oxidative stress.
研究领域:
兽医学
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药理学
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免疫学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）