Foods
·
2021
·Vol. 10
·期 8
·1942
·
IF 4.35
Ye Dong,Wen Yan,Xiao-Di Zhang,Zhi-Yuan Dai,Yi-Qi Zhang
The development of an efficient pretreatment, prior to enzymatic hydrolysis, is a good strategy for the sustainable use of refractory fish byproducts. This study compared hydrothermal pretreatments at 159 °C for 2 min, followed by water extraction (steam explosion-assisted extraction, SE) and 121 °C for 70 min (hot-pressure extraction, HPE), for the recovery of proteins from fish backbones. The effect of enzymatic hydrolysis on the properties of the obtained fish bone protein (FBP) was also evaluated. The results demonstrated that FBP had high contents of protein (81.09–84.88 g/100 g) and hydroxyproline (70–82 residues/1000 residues). After hydrolysis with Flavourzyme, for 3 h, the FBP hydrolysates that were pretreated with SE (SFBP-H) exhibited a better degree of hydrolysis (DH) and nitrogen recovery (NR), and a higher level of umami taste free amino acids (151.50 mg/100 mL), compared with the HPE-treated samples. The obtained SFBP-H mainly distributed below 3000 Da and had strong scavenging effects on 1,1-diphenyl-2-picrylhydrazy (DPPH) (IC50= 4.24 mg/mL) and 2,2-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) (IC50= 1.93 mg/mL) radicals. Steam explosion-assisted extraction is a promising route for recovering proteins from native fish bone materials, and improving the flavor and antioxidant activity of the hydrolysates.Keywords:fish backbone;protein;steam explosion;hot-pressure extraction;enzymatic hydrolysis
研究领域:
食品科学
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生物化学
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产品关联
DOI
PubMed
高影响因子
Waste and Biomass Valorization
·
2024
·
IF 2.6
Chen Ziqian,Gao Xiaoyan,Zhu Yihui,Deng Yuefeng,Wang Jianjun,Yang Chengli,Hu Kaishun,He Guanya,Li Dali
Food waste cuttlebone has the potential as the raw material of carrier for enzyme immobilization. In this study, we presented a method for synthesizing CaCO 3 nanoparticles coated chitosan (nano-CaCO 3 @chitosan) using cuttlebone as the raw material in the deep eutectic solvent of betaine and urea. The yield of nano-CaCO 3 @chitosan was 89.9%. The nano-CaCO 3 @chitosan was used for immobilization of α-amylase. The yield of enzyme protein was 65.6%. The loading of α-amylase on these nanoparticles was 49.1 mg/g. The optimum reaction conditions of the immobilized α-amylase were 80 °C and pH 4.0. The residual activity of the immobilized α-amylase was 82.9% after reused for 10 cycles. The raw materials for preparing nano-CaCO 3 @chitosan were almost from cuttlebone. The nano-CaCO 3 @chitosan was regarded as reassembly of cuttlebone. Therefore, food waste cuttlebone was utilized as bioresource. Graphical
研究领域:
生物技术
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食品科学
·
酶工程
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产品关联
DOI
高影响因子
Food Bioscience
·
2025
·
IF 5.9
Zixi Li,Meng Dong,Libo Qi,Shan Shang,Yan Guo,Lei Qin,Baoshang Fu
Frying is the necessary step of fish soup production, which directly affects the flavor and nutritional quality of the soup. To investigate the effect of edible oil on the final quality of fish bone soup, five kinds of oil were employed in this study. The results showed that olive oil could promote the dissolution of glutamic acid and aspartic acid into the soup, the umami value elevated nearly four times. Meanwhile, olive oil and sunflower oil brought more volatile flavor substances, which contributed to the highly accepted flavor. Triglyceride O-16:0/18:1/18:2 (palmitic/oleic/linoleic triglycerides) was regarded as a potential precursor for the formation of flavor substances, such as 1-octen-3-one and 2-heptanone, which contribute to the specific flavor of fish bone soup. This study revealed that frying would improve the quality of fish bone soup by changing the color and flavor profile, guiding the selection oil for the industrial processing of fish bone soup.
研究领域:
食品科学
·
代谢组学
·
营养学
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产品关联
DOI
高影响因子
JOURNAL OF BIOACTIVE AND COMPATIBLE POLYMERS
·
2021
·
IF 1.756
Xuqin Li,Zhou Lei,Jie Sheng,Yishan Song
In this study, a novel peptide grafted chitosan (CACS-FBP) with high peptide content, excellent moisture-absorption and moisture-retention abilities was prepared. Caffeic acid (CA) was used to modify chitosan, the highly water-soluble intermediate further reacted with fish bone collagen peptide to obtain the final product, and the synthesis of CACS-FBP was confirmed by the Fourier transform infrared spectroscopy (FT-IR), NMR, and UV-vis. The single-factor experiments indicated that the degree of substitution (DS) of CACS-FBP depended on the reaction temperature, reaction time, the mass ratio of fish bone collagen peptide to CACS (mFBP/mCACS) and the mass ratio of MTGase to CACS (mMTGase/mCACS). In addition, the antioxidant assay indicated that CACS-FBP had an excellent antioxidant capacity, and the CACS-FBP showed no cytotoxicity toward L929 mouse fibroblasts, all the results mean that the prepared peptide-containing chitosan derivative has potential application in pharmaceutical and biomedical fields.
研究领域:
生物化学
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药学
·
材料科学
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产品关联
DOI
高影响因子
APPLIED ORGANOMETALLIC CHEMISTRY
·
2017
·Vol. 32
·期 1
·e3946
·
IF 2.319
Xingquan Xiong,Zhongke Tang,Zhaohong Sun,Xiaoqing Meng,Sida Song,Zhilong Quan
An eco-efficient, green, and multi-gram procedure is presented for one-pot multicomponent synthesis of N -substituted 1,2,3-triazoles by using waste fishbone powders supported CuBr (FBPs-CuBr) as catalyst. FBPs-CuBr is found to be an efficient heterogeneous catalyst and a series of 1,2,3-triazoles are obtained in moderate to excellent yields in water under MW irradiation (70–98%). It can be separated conveniently by a simple filtration and reused at least seven consecutive runs with a slight drop in the product yields. Furthermore, the desired product still could be obtained in 80% yield when the scale of the reaction was increased to 40.0 mmol.
研究领域:
有机化学
·
绿色化学
·
催化材料
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DOI
高影响因子
Foods
·
2021
·Vol. 10
·期 6
·1409
·
IF 4.35
Yiqi Zhang,Ye Dong,Zhiyuan Dai
Bone hydrolysates from bighead carp (Aristichthys nobilis) were prepared using Protamex and Alcalase with degrees of hydrolysis (DH) of 5%, 10% and 15%. The antioxidant activity of bone hydrolysates was evaluated in vitro and then the hydrolysates with better antioxidant activity were used to immerse bighead carp fillets through a vacuum impregnation process at concentrations of 1% and 2%. Among the six hydrolysates, fish bone hydrolyzed with Protamex at DH 10% exhibited the highest ability to scavenge 1, 1-diphenyl-2-picrylhydrazyl (DPPH) (88.79%), 2, 2′-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS) (57.76%) and hydroxyl radicals (62.72%), as well as to chelate ferrous ions (91.46%). The hydrolysates effectively postponed freezing- and thawing-induced protein/lipid oxidation. Compared with the fillets without treatment, the impregnated fillets had higher sulfhydryl contents, greater Ca2+-ATPase activity, lower carbonyls and lower thiobarbituric acid-reactive substances (TBARS). Bone hydrolysates also have a positive effect on the texture and water-holding ability of freeze-thawed fish fillets. Fish bone hydrolysates of Protamex could serve as potential antioxidants to preserve fish fillets.Keywords:bone hydrolysates;antioxidant activity;fillet;vacuum impregnation;freeze–thaw cycles
研究领域:
食品科学
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生物化学
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产品关联
DOI
PubMed
高影响因子
RSC Advances
·
2019
·Vol. 9
·期 46
·27032-27041
·
IF 3.049
Ding Ding,Bowei Du,Chao Zhang,Fakhar Zaman,Yaqin Huang
To date, many researchers have developed active components that are derived from seafood processing for the purposes of healthcare. Here, an antioxidant collagen peptide was obtained from skipjack tuna (Katsuwonus pelamis) bone by using a combination of trypsin and chymotrypsin as the catalyst. The amino acid sequence of the peptide was identified as Ser–Ser–Gly–Pro–Pro–Val–Pro–Gly–Pro–Met–Gly–Pro–Met–Gly–Pro–Arg (SSGPPVPGPMGPMGPR) by liquid chromatography-electrospray ionization quadrupole time-of-flight mass spectrometry (LC-ESI-QTOF-MS) analysis. We found that the as-prepared collagen peptide can efficiently scavenge DPPH radical (IC50 3.149 mM), superoxide anion radical (IC50 3.803 mM) and ABTS radical (IC50 9.489 mM). In addition, it has been found that the methionine (Met) residue in the collagen peptide could provide a precise active site during the scavenging of DPPH radicals by Fourier transform infrared spectroscopy (FTIR) analysis and matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry analysis. These results suggest that the peptide can find wide uses in the food, cosmetic and pharmaceutical industries.
研究领域:
食品科学
·
生物化学
·
海洋生物资源利用
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产品关联
DOI
PubMed
高影响因子
FOOD RESEARCH INTERNATIONAL
·
2025
·
IF 8
Qiaoji Tian,Li Hao,Qian Zhang,Huilin Huang,Chaoxin Tu,Chaozhong Fan,Hu Hou
A novel cod bone peptide GETGPA with high calcium chelating capacity (4.60 ± 1.06 μg/mg) was prepared and synthesized to elucidate the binding mechanism of peptide with Ca 2+ . The chelation sites, binding modes, and coordination bond formation mechanism were systematically investigated by experimental methods combined with molecular dynamics simulation and density functional theory calculation. FTIR and Raman spectra showed that the oxygen atoms of carboxyl groups were likely involved in the binding of GETGPA with Ca 2+ . Furthermore, mass spectrometry identified Glu-2 as the primary calcium binding site of GETGPA. NMR indicated that the free carboxyl group of Ala-6 and Glu-2 might be participated in Ca 2+ chelation. There were multiple chelating modes in the system where multiple GETGPA molecules coexisted with Ca 2+ . The coordination bond formed between GETGPA and Ca 2+ exhibited excellent chemical and thermodynamic stability. This study provided new insights into the binding mechanisms of peptides and calcium.
研究领域:
生物化学
·
食品科学
·
计算生物学
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产品关联
DOI
PubMed
高影响因子
ULTRASONICS SONOCHEMISTRY
·
2023
·
IF 8.4
Wei He,Menglin Han,Ying Bu,Wenhui Zhu,Jianrong Li,Xuepeng Li
To study the physicochemical properties of micro-nanoparticles (MNPs) in thermoultrasonic treated fishbone soup, it was subjected to ultra-filtration with a 100 kDa ultrafiltration membrane to obtain large MNPs (LMNPs) and small MNPs (SMNPs). LMNPs and SMNPs were treated with force-breakers, and the interactions of the MNPs with five characteristic volatile compounds were investigated. LMNPs covered most proteins (222.66 mg/mL) and fatty acids (363.76 mg/g), while SMNPs was mostly soluble small molecules with taste substances like total free amino acids (85.26 mg/g), organic acids (2.55 mg/mL), and 5′-nucleotides (169.17 mg/100 mL). The stability of LMNPs is significantly higher than raw bone soup, and SMNPs can exist stably in the solution. Correlation analysis between flavor substance content and flavor suggested that the overall flavor profile of halibut bone soup was closely related to the content changes of 72 significant influence variables. The binding of LMNPs to characteristic flavor compounds was largely affected by hydrophobic interactions, hydrogen bonds, and ionic effects. While the binding of SMNPs to characteristic flavor compounds was largely determined by hydrophobic interaction and hydrogen bonding. This study explores the characteristics of MNPs and provides the possibility to clarify the interaction mechanism between MNPs and flavor.
研究领域:
食品科学
·
化学
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产品关联
DOI
PubMed
高影响因子
APPLIED CATALYSIS A-GENERAL
·
2025
·
IF 4.8
Zihan Yang,Yuqing Liu,Yida Wang,Xiaotong Sun,Yanzhi Zheng,Wanning Cao,Lixia Li,Liqin Wang,Hongli Zhao,Jingkai Yang
Photo-induced conversion of reactive oxygen species (ROS) has emerged as an effective means of removing persistent organic pollutants (POPs), but its key step of directed electron transfer from the active site to the stable O 2 molecule still possesses considerable challenges. In this work, we synthesized a novel 3D fishbone-like fluorinated BiVO 4 photocatalyst and explored the photocatalytic removal of dye-like species. The optimally fluorinated BiVO 4 with narrower band gap has an effective detoxification effect on MB and shows a degradation performance of 1.8 times higher than that of the original. Fluoride ions doped in the lattice and adsorbed on the surface lead to a slight increase of the electron density of BiVO 4 to elevate the conduction band potential and accelerate free radical activation. In addition, the degradation process of MB dye by F-BiVO 4 has been proposed. F-BiVO 4 material is proved as a promising reducing end of the F-BiVO 4 /g-C 3 N 4 heterojunction system.
研究领域:
环境科学
·
材料科学
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DOI
高影响因子
POLYMER SCIENCE SERIES A
·
2019
·Vol. 61
·期 5
·627-634
·
IF 0.984
Dakui Bie,Jiang Libin,Zhu Mengjie,Miao Weijun,Wang Zongbao
Abstract Bimodal polyethylene (BPE)/chitin nanocrystals (CNC) injection bars with various CNC content were prepared to explore the shish-kebab crystal structure of semi-crystalline polymer induced by bio-based nanofiller under flow field. The addition of CNC enhanced the ultimate tensile strength and Young’s modulus of the injection bars. Various characterization techniques were employed to investigate the mechanism of CNC reinforcing BPE injection bars. The wide-angle X-ray diffraction (WAXD) results showed that crystallinity and orientation were slightly increased with the 0.1–0.3 wt % CNC content but decreased in 0.5 wt %. The small-angle X-ray scattering (SAXS) results manifested that shish intensities were increased with the CNC content increasing, which suggests that more chains take part in the formation of shish-kebab crystals. The long period of shish-kebab with the CNC content was also increased firstly and then decreased with the CNC content increasing. The above results indicated that CNC plays a crucial role to promote shish-kebab formation. This research has an important theoretical significance in improving the mechanical properties of BPE industrial production.
研究领域:
材料科学
·
聚合物科学
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产品关联
DOI
高影响因子
ACS Applied Materials & Interfaces
·
2024
·
IF 8.3
Yan Wang,Song Wang,Zhiyan Sui,Yiman Gu,Yanchao Zhang,Jian Gao,Yijia Lei,Jialin Zhao,Na Li,JingYi Wu,Zhe Wang
N-Spirocyclic cations have excellent alkali resistance stability, and precise design of the structure of N-spirocyclic anion-exchange membranes (AEMs) improves their comprehensive performance. Here, we design and synthesize high-performance poly(triphenylene piperidine) membranes based on the “fishbone” design of amino/N-spirocyclic cations. The “fishbone” design does not disrupt the overall stabilized conformation but promotes a microphase separation structure, while exerting the synergistic effect of piperidine cations and spirocyclic cations, resulting in a membrane with good conductivity and alkali resistance stability. The hydroxide conductivity of the QPTPip-ASU-X membrane reached up to 133.5 mS cm–1 at 80 °C. The QPTPip-ASU-15 membrane was immersed in a 2 M NaOH solution at 80 °C for 1200 h, and the conductivity was maintained at 91.02%. In addition, the QPTPip-ASU-5 membrane had the highest peak power density of 255 mW cm–2.
研究领域:
材料科学
·
化学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）