JOURNAL OF ELECTROANALYTICAL CHEMISTRY
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2015
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IF 2.729
Ying Huang,Huijie Yan,Yuejin Tong
The electrocatalytic oxidation of glutathione based on rutin as a mediator was studied at acetylene black (AB) modified carbon paste electrode (CPE). The results of cyclic voltammetric experiments showed that rutin could catalyze oxidation of GSH in PBS (pH 7.0) and the oxidation current was greatly increased by using AB-CPE compared to the nonmodified CPE. The kinetic parameters such as catalytic rate constant and diffusion coefficient were obtained using double potential step chronoamperometry. Under the optimum conditions, the electrocatalytic oxidation peak current was linear to the glutathione concentration range from 0.5 μM to 3.5 μM and 2.5 μM to 25 μM with the detection limit of 0.080 μM (S/N = 3) by differential normal pulse voltammetry (DNPV). The proposed method has been applied to the determination of glutathione in pharmaceutical sample with satisfactory results.
研究领域:
分析化学
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生物化学
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电化学
✓ 已验证
DOI
高影响因子
International Journal of Electrochemical Science
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2023
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IF 1.5
Jing Leng,Ping Li,Ling Bai,Yingqiong Peng,Yongfang Yu,Limin Lu
A Pd nanoparticles-graphene oxide (PdNPs/GO) hybrid as a highly sensitive sensing platform for determination of rutin was successfully prepared by a facile one-pot redox synthesis approach. Formation of PdNPs and loading onto the GO nanosheets were accomplished simultaneously by a redox reaction between H2PdCl4 and GO, without use of any additional reductants. Scanning electron microscopy (SEM) results demonstrated that PdNPs with an average size of 60 nm were distributed on the surface of GO nanosheets. Compared with the GO modified electrode, the PdNPs/GO modified electrode showed a better electrocatalytic activity toward the oxidation of rutin with larger peak current. Then the PdNPs/GO hybrid can be used as an enhanced sensing platform for the electrochemical determination of rutin. Under the optimal conditions, the anodic peak current was linear to the concentration of rutin in the range from 0.005 to 6 μM with the detection limit of 0.001 μM (S/N = 3). More importantly, the developed sensor can be used for the determination of rutin in medicine tablets with satisfactory results.
研究领域:
材料科学
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分析化学
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药物分析
✓ 已验证
DOI
高影响因子
MICROBIAL PATHOGENESIS
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2021
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IF 3.738
Zhibin Wang,Zixuan Ding,Zhaoyinqian Li,Yinhuan Ding,Fan Jiang,Jinbo Liu
The emergence of multidrug resistance (MDR) and extensive drug resistance (XDR) in Klebsiella pneumoniae strains has posed great threats to conventional antibiotics. Previous studies have shown that plant-derived flavonoids have inhibitory functions against pathogens . However, in K . pneumoniae , the antibacterial activity of different flavonoids against growth and biofilm formation remains a mystery. The aim of the present study was to evaluate the antioxidant abilities of different flavonoids, to screen active ingredients and to identify their inhibitory effects on K . pneumoniae growth and biofilm formation. In total, 10 flavonoids representing 4 major categories were screened and used in this study. The antioxidant capacity of each flavonoid was evaluated through a DPPH (2,2-diphenyl-1-picrylhydrazyl) assay. Rutin showed the highest level of free radical scavenging capacity, followed by kaempferol , luteolin , quercetin , apigenin , hesperidin , sinensetin, naringenin , naringin and 3,5,6,7,8,3′,4′-heptamethoxyflavone. The inhibitory effects of rutin and naringin on bacterial growth were also compared. The lowest MICs of rutin were found against K. pneumoniae ATCC700603 (1024 μg/mL) and E. coli ATCC25922 (512 μg/mL). However, the MBICs were not found. Rutin showed strong inhibitory ability against both the growth curve and biofilm production. The expression profiles of 15 biofilm-related genes were analyzed in biofilm cells both with and without rutin treatment . The luxS gene and wabG gene were downregulated significantly by rutin treatment. Correlation analysis showed that mrkA gene expression was positively correlated with biofilm biomass accumulation. Our study indicated that biofilm production is correlated with the expression of several genes rather than one. MrkA gene expression was positively correlated with biofilm biomass accumulation. Our study screened rutin as a potential agent to inhibit K . pneumoniae biofilm formation.
研究领域:
微生物学
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药理学
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分子生物学
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DOI
PubMed
高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
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2024
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IF 9.4
Shengxiang Fu,Zhongyuan Cai,Haojie Gu,Su Lui,Hua Ai,Bin Song,Min Wu
Manganese oxide nanoparticles (MONs)-based contrast agents have attracted increasing attention for magnetic resonance imaging (MRI), attributed to their good biocompatibility and advantageous paramagnetism. However, conventional MONs have poor imaging performance due to low T 1 relaxivity. Additionally, their lack of tumor-targeting theranostics capabilities and complex synthesis pathways have impeded clinical applications. Rutin (Ru) is an ideal tumor-targeted ligand that targets glucose transporters (GLUTs) overexpressed in various malignant tumors, and exhibits photothermal effects upon chelation with metal ions. Herein, a series of Ru-coated MONs (Ru/MnO 2 ) were synthesized using a straightforward, rapid one-step process. Specifically, Ru/MnO 2 -5, with the smallest crystal size of approximately 4 nm, exhibits the highest T 1 relaxivity (33.3 mM -1 s −1 at 1.5 T, surpassing prior MONs) along with notable stability, photothermal efficacy, and tumor-targeting ability. Furthermore, Ru/MnO 2 -5 shows promise in MRI and photothermal therapy of H22 tumors owing to its superior GLUTs-mediated tumor-targeting capability.
研究领域:
医学影像学
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纳米医学
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肿瘤学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CARBON
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2018
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IF 7.082
Huiyu Li,Yuan Xu,Li Zhao,Jie Ding,Mengyang Chen,Guangrui Chen,Yi Li,Lan Ding
The solid-phase pyrolysis method is widely used in the synthesis of fluorescent carbon dots (CDs) because of its large scale and solvent-saving advantages. However, CDs are inclined to agglomerate during the synthesis process, resulting in low quantum yield (QY) and inhomogeneous distribution of particle sizes. Here, a simple and effective solid dispersion surface pyrolysis method was used for the synthesis of tiny-carbon dots (T-CDs) with a high QY. Multi-walled carbon nanotubes (MWCNTs) were used as a support and 2-( N -morpholino)ethanesulfonic acid (MES) as a precursor. MES was uniformly dispersed on the surface of MWCNTs by hydrogen bonding and π-π interactions. Due to the excellent thermal conductivity and rigidity of MWCNTs, the precursor is carbonized without agglomeration. Compared with the CDs obtained by the direct pyrolysis method without the use of a solid support, the particle size of the T-CDs is small (0.69 ± 0.14 nm) and the QY increased from 17.8 to 42.8%. It is anticipated that this method will lead to the improvement of the QY of CDs. Applications of the T-CDs included fluorescence detection of rutin in pharmaceuticals, human urine, and human serum samples, and determination of Al 3+ in tap water samples.
研究领域:
材料科学
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分析化学
✓ 已验证
产品关联
DOI
高影响因子
Frontiers in Pharmacology
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2021
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IF 5.811
Yang Xiao,Shao Jiang,Wu Xiao-Min,Pan Fei-Fei,Yang Shao-An,Pan Xiao-Hua,Jin An-Min
Troxerutin (TRX), a semi-synthetic derivative of the natural bioflavonoid rutin, is a bioactive flavonoid widely abundant in various fruits and vegetables. Known as vitamin P4, TRX has been demonstrated to have several activities including anti-inflammation, anti-oxidants, vasoprotection, and immune support in various studies. Although rutin, the precursor of troxerutin, was reported to have a protective role against bone loss, the function of TRX in skeletal system remains unknown. In the present study, we found that TRX promoted osteogenic differentiation of human mesenchymal stem cells (MSCs) in a concentration-dependent manner by stimulating the alkaline phosphatase (ALP) activity, calcium nodule formation and osteogenic marker genes expression in vitro. The further investigation demonstrated that TRX stimulated the expression of the critical transcription factor β-catenin and several downstream target genes of Wnt signaling, thus activated Wnt/β-catenin signaling. Using a femur fracture rats model, TRX was found to stimulate new bone formation and accelerate the fracture healing in vivo. Collectively, our data demonstrated that TRX could promote osteogenesis in vitro and facilitate the fracture healing in vivo, indicating that TRX may be a promising therapeutic candidate for bone fracture repair.
研究领域:
药理学
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细胞生物学
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骨科学
✓ 已验证
DOI
PubMed
高影响因子
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