AMINO ACIDS
·
2025
·Vol. 57
·期 1
·1-15
·
IF 2.4
Wang Jiteng,Zhang Jingyi,Li Xinyu,Xu Hanying Y.,Yang Yeshun,Zhang Jiankun,Feng Wenping,Chen Qiang,Dong Fen,Han Tao
The main objective of this experiment was to study the metabolism of arginine in juvenile largemouth bass ( Micropterus salmoides ). A total of 300 healthy fish (average weight of 25 ± 0.5 g) were randomly assigned to ten groups. Experimental fish were orally administered or intraperitoneally injected with 0.9% sodium chloride, arginine, arginine-aspartate, citrulline, and glutamate solutions, respectively. They were euthanized at 10, 30, 60, 120, and 240 min after oral administration or intraperitoneal injection, and various tissue samples were subsequently collected for analysis. The results revealed that serum ornithine and citrulline concentrations of largemouth bass were significantly increased by oral administration of arginine or arginine-aspartate ( P < 0.05). Intraperitoneal injection of arginine or arginine-aspartate solution significantly elevated the concentrations of ornithine and citrulline in the serum, liver, kidney, and muscles ( P < 0.05). The concentrations of citrulline, ornithine, and arginine in serum and muscle increased significantly at 4 h after intraperitoneal injection of glutamate ( P < 0.05). Intraperitoneal injection of citrulline significantly increased the concentrations of ornithine and arginine in the serum and muscles ( P < 0.05). The research findings demonstrate that both free and small peptide forms of arginine were rapidly degraded to ornithine due to the high arginase activity in various tissues of largemouth bass. Additionally, the pathway of synthesizing citrulline from glutamate and then arginine from citrulline may exist in largemouth bass, but the exact location of this synthesis process may differ from that found in mammals.
研究领域:
水产养殖学
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生物化学
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产品关联
DOI
PubMed
高影响因子
Viruses-Basel
·
2022
·Vol. 14
·期 7
·1421
·
IF 5.818
Yingjie Jin,Fei Yang,Gengrong Zhang,Qing Yu,Gaoxue Wang,Fei Ling,Tianqiang Liu
Micropterus salmoidesrhabdovirus (MSRV) is a primary viral pathogen in largemouth bass aquaculture, which leads to tremendous economic losses yearly. Currently, there are no approved drugs for the treatment and control of this virus. Our previous studies screened the herbMagnolia officinalisfrom many traditional Chinese medicines, and we isolated and identified magnolol as its main active compound against multiple rhabdoviruses, including MSRV. On the basis of the structure–activity relationship and pharmacophore model of magnolol, two new magnolol derivatives, namely, hydrogenated magnolol and 2,2′-dimethoxy-magnolol, were designed and synthesized. Their anti-MSRV activities were systematically investigated both in vitro and in vivo. By comparing the half-maximal inhibitory concentration (IC50), it was found that hydrogenated magnolol possessed a higher anti-MSRV activity than magnolol and 2,2′-dimethoxy-magnolol, with an IC50of 13.37 μM. Furthermore, hydrogenated magnolol exhibited a protective effect on the grass carp ovary (GCO) cell line by reducing the cytopathic effect induced by MSRV. Further studies revealed that hydrogenated magnolol did not directly impact virions or interfere with MSRV adsorption. It worked within the 6–8 h of the phase of virus replication. In vivo treatment of MSRV infection with magnolol and hydrogenated magnolol showed that they significantly improved the survival rate by 44.6% and 62.7%, respectively, compared to MSRV-infected groups. The viral load measured by the expression of viral glycoprotein in the organs including the liver, spleen, and kidney also significantly decreased when fish were intraperitoneally injected at a dose of 20 mg/kg. Altogether, the structural optimization of magnolol via hydrogenation of the propylene groups increased its anti-MSRV activity both in vitro and in vivo. These results may provide a valuable reference for anti-MSRV drug discovery and development in aquaculture.Keywords:Micropterus salmoidesrhabdovirus (MSRV);magnolol;largemouth bass;antiviral activity
研究领域:
水产养殖学
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病毒学
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药物化学
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DOI
PubMed
高影响因子
JOURNAL OF FISH DISEASES
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2022
·Vol. 45
·期 11
·1599-1607
·
IF 2.58
Xin-Yuan Jiang,Zong-Yi Ren,Nian-Kun Zhang,Ke-Chen Yang,Gao-Xue Wang,Hai-Feng Jiang
Nocardiosis caused by Nocardia seriolae is a major threat to the aquaculture industry. Given that prolonged therapy administration can lead to a growth of antibiotic resistant strains, new antibacterial agents and alternative strategies are urgently needed. In this study, 80 medicinal plants were selected for antibacterial screening to obtain potent bioactive compounds against N. seriolae infection. The methanolic extracts of Magnolia officinalis exhibited the strongest antibacterial activity against N. seriolae with the minimal inhibitory concentration (MIC) of 12.5 μg/ml. Honokiol and magnolol as the main bioactive components of M. officinalis showed higher activity with the MIC value of 3.12 and 6.25 μg/ml, respectively. Sequentially, the evaluation of antibacterial activity of honokiol in vivo showed that honokiol had good biosafety, and could significantly reduce the bacterial load of nocardia-infected largemouth bass ( p < .001). Furthermore, the survival rate of nocardia-infected fish fed with 100 mg/kg honokiol was obviously improved ( p < .05). Collectively, these results suggest that medicinal plants represent a promising reservoir for discovering active components against Nocardia , and honokiol has great potential to be developed as therapeutic agents to control nocardiosis in aquaculture.
研究领域:
水产养殖学
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药用植物学
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微生物学
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Zi-Chun Zhang,Ji Wang,Meng Dong,Shuang Cui,Xu-Hui Huang,Lei Qin
Salt enhances flavor and salinity in Chinese curing; however, excessive use can pose health risks, while reducing NaCl may harm taste. This study utilized targeted and untargeted metabolomics to investigate the intrinsic molecular mechanisms that drive flavor formation in cured sea bass subjected to salt. Glycine, succinic acid, lactic acid and uridine significantly contributed to the taste profile of the cured sea bass. A total of 668 lipid molecules were annotated in the samples, of which 60 were classified as differential lipids. Non-targeted lipidomic analysis identified phosphatidylcholine and phosphatidylethanolamine as the major flavor precursors, constituting 40.12 % of the total. Elevated salt concentrations significantly enhanced the production and accumulation of key differential volatile flavor components, including 1-octen-3-ol, 2-undecanone and 2-pentylfuran. Thus, salt facilitated the degradation and oxidation of lipids, leading to the formation of key flavor compounds that contribute to the enhancement of the flavor profile of cured sea bass.
研究领域:
食品科学
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生物化学
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
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2022
·Vol. 57
·期 12
·7917-7928
·
IF 3.612
Yu Liu,Zheming Wang,Yizhen Huang,Kunihiko Konno,Tianjian Tong,Chenxu Yu,Beiwei Zhu,Xiuping Dong
Summary The dark muscle of yellowtail kingfish is regularly consumed as fresh sashimi alongside with the ordinary muscle. Thus, in this study, three types of ATPase activities, chymotryptic digestion patterns as well as thermal unfolding of various myosin domains were determined to reveal the denaturation characterisation of myosin in dark muscle and compare it with normal muscle. The thermal inactivation rate of dark muscle myosin Ca 2+ -ATPase at 30°C (7.3 × 10 −4 , sec −1 ) was significantly ( P < 0.05) greater than that of ordinary muscle (5.9 × 10 −4 , sec −1 ), while dark muscle myosin became more stable than ordinary muscle one once bound with F-actin. On the other hand, the rod region especially light meromyosin region of the dark muscle myosin was much more stable than ordinary myosin. These results characterised that myosin in the dark muscle of yellowtail kingfish is constituted with less stable head and more stable rod regions than those in the ordinary muscle.
研究领域:
生物化学
·
肌肉生理学
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DOI
高影响因子
ANIMAL FEED SCIENCE AND TECHNOLOGY
·
2025
·
IF 2.7
Weijun Chen,Shiyang Gao,Ping Sun,Lei Han,Zhenyang Zhang
The investigation was to validate the alleviating effect of sodium butyrate on high-fat diet-induced gut injury and growth inhibition in largemouth bass ( Micropterus salmoides ). Five diets were designed: four high-fat diets (18 % crude lipid) supplemented with sodium butyrate at 0 % (HF), 0.05 % (HFS1), 0.1 % (HFS2), and 0.2 % (HFS3), as well as a control diet (C, 10 % crude lipid). Three tanks (30 fish each tank) were randomly allocated to each diet. Fish (4.0 g) were fed to apparent satiation for sixty days. Results indicated that HF dramatically reduced fish growth and induced oxidative stress, mucosal barrier dysfunction, gut inflammation, and gut dysbiosis compared to C. However, in comparison to HF, HFS1, HFS2, and HFS3 diets significantly promoted feed intake and weight gain rate, relieved oxidative stress by increasing antioxidant defense (e.g., total superoxide dismutase, catalase, glutathione peroxidase, reduced glutathione, and nuclear factor E2-related factor 2) and reducing malondialdehyde content, enhanced mucosal barrier by upregulating the expression of occludin, mucin3a, zona occluding-1, hypoxia-inducible factor-1α, and lysozyme, and reduced inflammation by downregulating the expression levels of interleukin 1β and tumor necrosis factor α ( P < 0.05). Moreover, HFS1 and HFS3 dramatically reduced Tenericutes and Mesomycoplasma abundance, whereas HFS1 increased Lactococcus abundance when compared to HF ( P < 0.05). Correlation analysis revealed a significant relationship between fish growth and gut injury with mucosal barrier proteins and gut microbiota (e.g., Tenericutes, Mesomycoplasam , and Lactococcus ) ( P < 0.05). These findings suggested that sodium butyrate can help mitigate the detrimental effects of high-fat diets on fish growth and gut health.
研究领域:
水产养殖学
·
营养学
·
微生物学
✓ 已验证
产品关联
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2023
·
IF 4.8
Wenqian Yan,Jiayi Yao,Zilin Yue,Hong Lin,Lei Wang,Kaiqiang Wang,Jinjie Li
TVB-N is a commonly used chemical indicator for assessing the freshness of aquatic products during storage. In this study, the combination of Raman spectroscopy with multivariate analysis for monitoring the freshness of sea bass ( Lateolabrax japonicus ) fillets was investigated. Orthogonal signal correction (OSC) method was applied to pre-process the Raman spectra of sea bass fillets with different freshness levels. Results suggested that PLSR models outperformed PCR and SVMR models in monitoring TVB-N values of sea bass fillets. In comparison, the OSC-PLSR model with full-band Raman spectra showed the best performance for predicting TVB-N contents, with R 2 C of 0.981, RMSEC of 1.162 mgN/100 g, R 2 CV of 0.804, RMSECV of 3.756 mgN/100 g, R 2 P of 0.898, RMSEP of 2.199 mgN/100 g, and RPD of 3.824. In addition, sea bass fillets with three different freshness grades (first-grade, second-grade, and spoilage) could be distinguished from the PCA score plot. The PLS-DA model showed an average classification accuracy of 91.11% for different freshness grades. The combination of Raman spectroscopy and chemometrics provided a new tool for the non-destructive evaluation of fish quality.
研究领域:
食品科学
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光谱学
·
化学计量学
✓ 已验证
DOI
高影响因子
AQUACULTURE
·
2022
·
IF 5.135
Weijun Chen,Shiyang Gao,Kuo Chang,Xiaoyu Zhao,Ben Niu
The present study evaluated the effect of dietary sodium butyrate (NaBT) supplementation on the growth performance, intestinal microbiota composition, and liver health of largemouth bass Micropterus salmoides fed high-fat diets. Four isonitrogenous and isolipid diets were formulated: a high-fat diet and the high-fat diet supplemented by 0.05% NaBT, 0.1% NaBT, or 0.2% NaBT. Each diet was randomly distributed to triplicate tanks (200 L ) containing 30 fish (initial weight 4.0 ± 0.1 g) per tank. The experimental fish were fed to apparent satiation twice daily for 60 days. The orthogonal polynomial contrasts showed that the weight gain rate (WGR) and specific growth rate (SGR) increased linearly ( P < 0.05) with increasing dietary NaBT levels (P < 0.05). No significant differences were found for feed intake (FI), feed conversion ratio (FCR), condition factor (CF), viscerosomatic index (VSI), and hepatosomatic index (HSI). There were no differences in the alpha diversity indices (including Operational Taxonomic Units, Chao index, and Simpson index) of intestinal microbiota between the various dietary groups ( P > 0.05). For the intestinal microbiota composition, the proportion of Proteobacteria were linearly and cubically increased, whereas the proportion of Tenericutes and Mesomycoplasma were linearly and cubically decreased with the increase of dietary NaBT levels ( P < 0.05). The increasing dietary NaBT levels led to significantly quadratic decrease of alanine aminotransferase (ALT) ( P < 0.05), linear and quadratic decrease of aspartate aminotransferase (AST) ( P < 0.05), and linear, quadratic, and cubic decrease of diamine oxidase (DAO) ( P < 0.05). Hepatic triglyceride (TG) and total cholesterol (TCHO) contents decreased linearly and quadratically with the increase of dietary NaBT levels ( P < 0.05). Increasing dietary NaBT levels linearly, quadratically, and cubically decreased the malondialdehyde (MDA) contents ( P < 0.05). Total superoxide dismutase (T-SOD) and glutathione peroxidase (GPx) activity levels increased linearly with the increasing dietary NaBT levels ( P < 0.05). As the dietary NaBT levels gradually increased, the relative expression of carnitine palmitoyl transferase (CPT1) and peroxisome proliferator-activated receptor α (PPARα) increased linearly, quadratically, and cubically ( P < 0.05), while the relative expression of sterol regulatory element binding protein 1 (SREBP 1) decreased linearly, quadratically, and cubically ( P < 0.05). The relative expression of tumor necrosis factor α (TNF-α) and cysteinyl aspartate specific protease 3 (Caspase 3) decreased linearly and quadratically with the increasing NaBT levels ( P < 0.05). Pearson correlation analysis showed that fish growth was closely correlated with microbiota composition, hepatic lipid accumulation, hepatic antioxidant-related parameters, and hepatic gene expression ( P < 0.05). These results indicated that NaBT could be used to alleviate the negative influence of high-fat diets on fish growth, intestinal microbiota homeostasis , and liver health.
研究领域:
水产养殖学
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营养学
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微生物学
✓ 已验证
产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Xuening Chen,Weiqing Lan,Jing Xie
The poor mechanical properties, low water-resistance, and limited antimicrobial activity of chitosan (CS)/polyvinyl alcohol (PVA) based film limited its application in aquatic product preservation. Herein, bacterial cellulose (BC) was used to load ginger essential oil (GEO). The effects of the addition of BC and different concentrations of GEO on the physicochemical and antimicrobial activities of films were systematically evaluated. Finally, the application of sea bass fillets was investigated. Fourier transform infrared spectroscopy (FTIR) and X-ray diffraction analysis (XRD) analysis indicated dense networks were formed, which was verified by enhanced physical properties. The mechanical properties, barrier properties, and antimicrobial activities enhanced as GEO concentration increased. CPB 0.8 (0.8 % GEO) film had better tensile strength (TS) and barrier performance, improved the quality, and extended the shelf-life of sea bass for another 6 days at least. Overall, active films are potential packaging materials for aquatic products.
研究领域:
食品科学
·
材料科学
✓ 已验证
DOI
PubMed
高影响因子
PACKAGING TECHNOLOGY AND SCIENCE
·
2024
·
IF 2.8
Haitao Huang,Zhaomeng Xv,Yu Qiao,Yonghong Ge,Xiuxia Li,Qiuying Li,Tong Sun
In order to inhibit the growth and reproduction of microorganisms in the exudate of fresh aquatic products during storage. Absorbent paper, absorbent cotton and nylon mesh were used as the substrates, three types of clay minerals (diatomite, columnar diatomite and bentonite) were used as the water absorbing materials and a new type of unidirectional water-conducting preservation pads was prepared. The physicochemical properties of water absorbing materials and preservation properties of pads for Lateolabrax japonicas fillets were measured. The results show that all three kinds of clay have good water absorption properties, and bentonite exhibits the highest water retention properties because of metal oxide. After absorbing water, the surface holes of diatomite and columnar diatomite particles are blocked, while the volume of bentonite particles increases. Bentonite absorbs water slowly but retains water best and can absorb 10.31 times its own mass of water. Preservation study shows that water absorption preservation pads can effectively absorb the exudate of fish fillets and slow down the deterioration of fish fillet quality. Bentonite preservation pad shows the best effective and extends the shelf life of L. japonicas fillets from 6 to 9.3 days. This research can provide the technical support for the application of preservation pads carrying clay minerals in the field of fresh aquatic products.
研究领域:
食品科学
·
水产保鲜技术
✓ 已验证
产品关联
DOI
高影响因子
AQUACULTURE RESEARCH
·
2021
·Vol. 52
·期 7
·2963-2979
·
IF 2.082
Qin Ni,Chunfang Cai,Shengjie Ren,Junbiao Zhang,Yijun Zhao,Xinyi Wei,Yiyang Ge,Chengrui Wang,Wenjian Li,Ping Wu,Yuantu Ye,Xiamin Cao
This study aimed to reveal the impact of excessive dietary fibre (DF) intake on inflammatory responses and bile acid (BA) homeostasis in largemouth bass ( Micropterus salmoides ) and the potential mechanism. In vitro BA binding capacity was compared, and the results showed that the BA binding capacity to chenodeoxycholic acid and glycocholic acid by pectin and soybean meal was higher than cellulose and cottonseed meal respectively. An in vivo trial was conducted using a diet without DF as a control (CON diet), while test diets contained 150 g/kg cellulose (CEL diet) or pectin (PEC diet), 500 g/kg soybean meal (SBM diet) or cottonseed meal (CSM diet). Fish were overfed and ate ad libitum . Compared to fish fed the CON diet, fish fed the PEC and SBM diets showed high expression of inflammatory factor genes and BA synthesis factor genes and obvious histopathological damage in the gut and liver, while the responses of fish fed the CEL and CSM diets were mild. Whole-body BA contents in fish fed all test diets were lower than those in fish fed the CON diet ( p < 0.05). These results support the hypothesis that excessive DF intake induces inflammatory responses and dysregulation of BA homeostasis, which might be attributed to their binding capacity to BAs.
研究领域:
水产养殖
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营养学
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分子生物学
✓ 已验证
产品关联
DOI
高影响因子
AQUACULTURE RESEARCH
·
2023
·
IF 2
Chunjing Cai,Zhe Li,Junhua Lu,Ying Wang,Chen Wang,Pengfei Li,Hui Wang,Wu Meng,Jie Chu
The purpose of this study was to investigate the effects of dietary acetoin on Largemouth Bass (Micropterus salmoides). In 45 d of farming, the experimental diets were supplemented with 0%, 0.3%, 0.6%, and 0.9% of acetoin levels, respectively. Results showed that the activities of lipase (LPS), amylase (AMS), and trypsin in fish fed with acetoin at 0.6% displayed the most obvious improvement compared with the other levels of acetoin (). Furthermore, the incorporation of acetoin at 0.3% and 0.6% in Micropterus salmoides diet significantly enhanced the number of beneficial bacteria (such as Cetobacterium) and reduced the number of harmful bacteria (such as Proteobacteria) in the gut flora of Micropterus salmoides. Meanwhile, the activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-PX), and catalase (CAT) in the liver of the samples treated with acetoin at 0.6% were significantly higher than the control (), while the content of malondialdehyde (MDA) and the activities of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in the liver notably decreased with the increase of the addition amount of acetoin (). Furthermore, the contents of IgA, IL-6, and TNF-α and the activity of acid phosphatase (ACP) in the serum of the samples added with acetoin exhibited a concentration-dependent decline compared with the control (). In summary, acetoin had potential feeding attraction effects on Micropterus salmoides, and the most suitable dose of dietary application was 0.6% acetoin, which can effectively improve the physiological characteristics and immune activity of Micropterus salmoides.
研究领域:
水产养殖学
·
动物营养学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）