FOOD CHEMISTRY
·
2024
·
IF 8.5
He-He Tian,Yu-Lian Li,Xu-Song Wang,Xu-Hui Huang,Yu-Ying Zhang,Zi-Chun Zhang,Da-Yong Zhou,Lei Qin
Abalone, a highly sought-after aquatic product, possesses significant nutritional value. In this study, the relationship between aroma characteristics and lipid profile of abalone ( Haliotis discus hannai ) during seasonal fluctuation and thermal processing were profiled via volatolomics and lipidomics. 46 aroma compounds and 371 lipids were identified by HS-SPME-GC–MS and UPLC-Q-Extractive Orbitrap-MS, respectively. Multivariate statistical analysis indicated that carbonyls (aldehydes and ketones) and alcohols were the characteristic aroma compounds of abalone. The fluctuations in the aroma compound and lipid composition of abalone were consistent with the seasonal variation, especially seawater temperature. In addition, based on the correlation analysis, it was found that carbonyls (aldehydes and ketones) and alcohols had a positive correlation with phospholipids (lysophosphatidylethanolamines and lysophosphatidylcholines), while a negative correlation was observed with fatty acyls. These findings suggested that the effect of seasonal variations on the aroma changes of abalone might achieved by modulating the lipids composition of abalone.
研究领域:
食品科学
·
生物化学
✓ 已验证
DOI
PubMed
高影响因子
FISHERIES SCIENCE
·
2014
·Vol. 80
·期 5
·1097-1107
·
IF 0.855
Wang Hong-Xu,Qin Lei,Wang Yan,Zhou Da-Yong,Song Shuang,Wang Xu-Song,Zhu Bei-Wei
Abalone Haliotis discus hannai Ino is a popular delicacy consumed as a luxury food owing to its unique flavor and texture. In this study, we investigated the effects of heating conditions on the fatty acids and volatile compounds in its foot muscle based on gas chromatography–mass spectrometry (GC–MS) and solid-phase microextraction (SPME)–GC–MS. The contents of fatty acids significantly decreased after heating at 80 °C for 2 h. In total, 52 volatile compounds, including aldehydes, aromatic compounds, alkanes, alcohols, ketones, and furans, were detected in the heated samples. Principal component analysis revealed an interaction between heating temperature and time. Heating at 80 °C for 0.5–2 h generated higher contents of volatile compounds. In particular, the contents of hexanal, heptanal, octanal, nonanal, and undecanal mainly derived from autoxidation of fatty acids during heating increased at least fourfold.
研究领域:
食品科学
·
生物化学
✓ 已验证
DOI
高影响因子
JOURNAL OF FOOD SCIENCE
·
2020
·Vol. 85
·期 12
·4260-4270
·
IF 2.479
Shu Guo,Jiao Wang,Chuanbo He,Haocheng Wei,Ying Ma,Hejian Xiong
Abalone viscera were byproducts of the abalone processing and usually discarded as wastes. In this study, we tried to obtain functional polysaccharides from abalone viscera by a combination of enzymatic hydrolysis, membrane separation, anion exchange chromatography, and gel filtration techniques. Abalone viscera underwent successive hydrolyzation with alcalase and flavourzyme. Each enzymolysis was followed by deproteinization via membrane separation. The final yield of crude abalone viscera polysaccharide (CAVP) was 19.72%; the polysaccharide content of CAVP was 51.75%. Furthermore, three fractions of polysaccharides (AVP1, AVP2, and AVP3) were isolated from the CAVP by anion exchange chromatography and gel filtration. The molecular weights of each AVP were 14.99 kDa, 58.48 kDa, and 39.63 kDa, with a carbohydrate content of 62.75, 23.09, and 44.67%, respectively. These AVPs showed excellent antioxidant activities in vitro. Our results provide a scientific basis for the further utilization of polysaccharides from abalone viscera. Practical Application This study demonstrated an eco-friendly approach for industrial production of high purity animal-derived polysaccharides without any environmental pollution caused by the viscera waste of abalone and promoting the comprehensive utilization of abalone resources.
研究领域:
食品科学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Functional Foods
·
2017
·
IF 3.144
Chunqing Ai,Na Ma,Xiaona Sun,Mengmeng Duan,Sufeng Wu,Jingfeng Yang,Chengrong Wen,Shuang Song
Various bioactivities of sulfated polysaccharides have been demonstrated, but the exact mechanisms involved are still unclear, which can be mainly attributed to a lack of recognition of polysaccharides metabolism in vivo . To define action mechanisms of sulfated polysaccharide from pacific abalone (AGSP), its metabolic characteristics were first evaluated in a mice model. The result showed that AGSP was hard to be absorbed into plasma, and most might be excreted from the body via feces. The in vitro models showed that human digestive juices had no effects on AGSP, and AGSP could exert its bioactivities by modulating the gut microbiota composition. It was noteworthy that small AGSP fragment was also hard to be absorbed by the body. In summary, this study demonstrated that various bioactivities of AGSP could be mainly attributed to the modulation of the gut microbiota rather than direct action on body organs far away from the digestive tract.
研究领域:
海洋生物学
·
营养学
·
微生物学
✓ 已验证
DOI
高影响因子
FOOD RESEARCH INTERNATIONAL
·
2019
·
IF 3.579
Jun Chen,Wenqi Cao,Peixiao Wei,Ting Li,Wuyin Weng
Speciation transformation of arsenic in the abalone viscera hydrolysate fraction (AVHF) was evaluated using in vitro and in vivo methods to determine its safety given that AVHF is rich in arsenic. The dimethylarsinic acid (DMA) proportion and some free amino acid contents increased, whereas arsenobetaine (AB) proportion decreased when AVHF was digested by pepsin. However, molecular weight distribution was unchanged, and no obvious changes were found in the intestinal medium. In the single-dose experiment, the AB concentration on the mouse plasma rapidly increased, which reached up to 12.53 ng/mL in 2 h after the administration of AVHF (10 g/kg body weight) and reduced to half of the maximum at 8 h after administration. Furthermore, alanine (Ala) content in the urine of mice increased at 8 h after AVHF administration, suggesting that Ala might be chelated with arsenic and could not be absorbed well. Long-term experiments showed that AB was not accumulated in mice tissue/organ. However, some AB could be converted into DMA, which was mainly accumulated in mice hair. The in vivo experiments also suggested that the AVHF is safe as health food.
研究领域:
营养学
·
毒理学
·
食品科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2018
·
IF 4.946
Yu-Ying Zhang,Lei Qin,Yu-Xi Liu,Da-Yong Zhou,Xian-Bing Xu,Ming Du,Bei-Wei Zhu,Megan Thornton
Abalone has been farmed commercially for the last few decades, and the breeding and economic value of abalone have gained extensive attention. In this study, the lipid profile of the foot muscles, viscera, and gonads of male and female Japanese abalone Haliotis discus hannai Ino was explored using ultra performance liquid chromatography-electrospray ionization-quadrupole time-of-flight mass spectrometry (UPLC-ESI-Q-TOF-MS), to discuss the effect of lipid composition on its nutritional value. Thirty-four species from ten lipid classes including phosphatidylcholine, phosphatidylethanolamine, phosphatidylserine, phosphatidylglycerol, phosphatidic acid, lysophosphatidic acid, triacylglycerol, steroids, terpenoids and fatty acids were annotated by MS-DIAL to obtain all fragment ions for precursors. Glycerophospholipids (GPLs) enriched in unsaturated fatty acids were the major components, which accounted for 52–57% of total lipids. Considering the high-level of GPLs, and their importance in maintaining the integrity and functionality of cell membranes, further utilization of inexpensive tissues, such as viscera and gonads is warranted.
研究领域:
水产养殖学
·
营养学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
He-He Tian,Yu-Lian Li,Xu-Song Wang,Xu-Hui Huang,Yu-Ying Zhang,Zi-Chun Zhang,Da-Yong Zhou,Lei Qin
Abalone ( Haliotis spp.) is a shellfish known for its exceptional nutritional value and significant economic worth. This study investigated the dynamic characteristics of non-volatile compounds over a year, including metabolites, lipids , nucleotides, and free amino acids (FAAs), which determined the nutritional quality and flavor of abalone. 174 metabolites and 371 lipids were identified and characterized, while 20 FAAs and 11 nucleotides were quantitatively assessed. These non-volatile compounds of abalone were fluctuated with months variation, which was consistent with the fluctuations of environmental factors, especially seawater temperature. Compared with seasonal variation, gender had less influence on these non-volatiles. June and July proved to be the optimal harvesting periods for abalone, with the levels of overall metabolites, lipids, FAAs, and nucleotides in abalone exhibiting a higher value in June and July over a year. Intriguingly, taurine covered 60% of the total FAAs and abalone could be used as dietary taurine supplementation.
研究领域:
海洋生物学
·
营养学
·
代谢组学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Marine Drugs
·
2019
·Vol. 17
·期 4
·244
·
IF 3.772
Fang Gong,Mei-Fang Chen,Yuan-Yuan Zhang,Cheng-Yong Li,Chun-Xia Zhou,Peng-Zhi Hong,Sheng-Li Sun,Zhong-Ji Qian
Vasculogenic mimicry (VM) formed by tumor cells plays a vital role in the progress of tumor, because it provides nutrition for tumor cells and takes away the metabolites. Therefore, the inhibition of VM is crucial to the clinical treatment of tumors. In this study, we investigated the anti-tumor effect of a novel peptide, KVEPQDPSEW (AATP), isolated from abalone (Haliotis discus hannai) on HT1080 cells by migration, invasion analysis and the mode of action. The results showed that AATP effectively inhibited MMPs by blocking MAPKs and NF-κB pathways, leading to the downregulation of metastasis of tumor cells. Moreover, AATP significantly inhibited VM and pro-angiogenic factors, including VEGF and MMPs by suppression of AKT/mTOR signaling. In addition, molecular docking was used to study the interaction of AATP and HIF-1α, and the results showed that AATP was combined with an active site of HIF-1α by a hydrogen bond. The effect of AATP on anti-metastatic and anti-vascular in HT1080 cells revealed that AATP may be a potential lead compound for treatment of tumors in the future.Keywords:abalone;peptide;vasculogenic mimicry;metastasis;MMPs;HIF-1α
研究领域:
肿瘤学
·
药理学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Aquatic Food Product Technology
·
2019
·
IF 0.707
Wuyin Weng,Jieyu Li,Ting Li,Yanjun Ye
A fraction with molecular weight of 0.2–1.0 kDa was prepared from abalone viscera hydrolysate by ultrafiltration and nanofiltration. The contents of protein, carbohydrate, and fat in abalone viscera were 45.75%, 18.19%, and 13.60%, respectively. The protein in abalone viscera hydrolysate faction (AVHF) reached up to 75.73%, while carbohydrate and fat fell to 1.80% and 0.20%, respectively. The leucine (Leu) content was the highest in the AVHF. On the other hand, the hydroxyl and 2,2-diphenyl-1-picrylhydrazyl radical scavenging activities and reducing power increased with increased concentration of AVHF. The inhibiting ability of AVHF on lipid peroxidation was higher than that of ascorbic acid. The total content of K, Na, and P in AVHF was 9.78 g/100 g, and total arsenic content reached 16.23 mg/kg. The proportion of arsenobetaine and arsenocholine accounted for more than 96%, while the proportion of arsenate was 0.47%, and arsenite was not detectable. It is concluded that the AVHF from abalone viscera is a safe natural antioxidant.
研究领域:
食品科学
·
营养学
·
毒理学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF SUPERCRITICAL FLUIDS
·
2019
·
IF 3.481
Gengxin Hao,Wenqi Cao,Ting Li,Jun Chen,Jinli Zhang,Wuyin Weng,Kazufumi Osako,Huifeng Ren
The effect of temperature on the main components and antioxidant activities of abalone viscera subcritical water extract (AVSWE) was investigated. With the increase in temperature from 110 °C to 230 °C, the protein content in AVSWE increased from 57.4% to 68.5%, while the carbohydrate content decreased from 17.9% to 0.8%. The highest percentage of 1000–5000 Da peptide fraction and total essential amino acid content was found in the AVSWE extracted at 170 °C. The transformation rate of arsenobetaine into dimethylarsinic acid in AVSWE increased dramatically with the increase subcritical water temperature from 170 °C to 200 °C. The results of DPPH and hydroxyl radical scavenging activity, reducing power and lipid peroxidation inhibition effect showed that AVSWE with the highest antioxidant activities was extracted at 170 °C. However, the subcritical water temperature for AVSWE extraction should not exceed 140 °C according to arsenic speciation.
研究领域:
食品科学
·
生物化学
✓ 已验证
DOI
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2019
·
IF 3.571
Fang Gong,Mei-Fang Chen,Jiali Chen,ChengYong Li,ChunXia Zhou,PengZhi Hong,ShengLi Sun,Zhong-Ji Qian
Abalone (Haliotis discus hannai) is a precious seafood in the market. It has been reported that biological active substances derived from abalone have anti-oxidative, anti-inflammatory, anti-bacterial, and anti-thrombosis potential. However, there were few studies to assess whether they have anti-cancer potential. In this study, we evaluated the anti-metastasis and anti-pro-angiogenic factors and mechanism of action of boiled abalone byproduct peptide (BABP, EMDEAQDPSEW) in human fibrosarcoma (HT1080) cells and human umbilical vein endothelial cells (HUVECs). The results demonstrated that BABP treatment significantly lowers migration and the invasion of HT1080 cells and HUVECs. BABP inhibits phorbol 12-myristate 13-acetate (PMA)-induced matrix metalloproteinase (MMP) expression and activity by blocking mitogen-activated protein kinases (MAPKs) and NF-κB signaling and hypoxia-induced vascular endothelial growth factor (VEGF) secretion and hypoxia inducible factor (HIF)-1α accumulation through suppressing the AKT/mTOR signal pathway. BABP treatment inhibits VEGF-induced VEGFR-2 expression and tube formation in HUVECs. The effect of BABP on anti-metastatic and anti-vascular activity in HT1080 cells and HUVECs revealed that BABP may be a potential pharmacophore for tumor therapy in the future.
研究领域:
肿瘤学
·
药理学
·
细胞生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）