INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Shaoyang Zhi,Junli Wang,Yiran Wang,Yijie Li,Mengjuan Zhao,Liping Yang,Chaobin Qin,Xiao Yan,Guoxing Nie
AMPKα1 and AMPKα2, key kinases in regulating energy homeostasis, have not been previously cloned or characterized in common carp ( Cyprinus carpio ). This study identified the open reading frame (ORF) sequences of ampkα1 (1722 bp, encoding 573 amino acids) and ampkα2 (1659 bp, encoding 552 amino acids) through homologous cloning. Sequence alignment and phylogenetic analysis showed a high similarity of both genes with fish homologs. Expression analysis revealed that ampkα1 and ampkα2 are widely expressed across tissues in carp, with ampkα1 highly expressed in the gonads and ampkα2 in the heart. Fasting significantly reduced ampkα1 expression in the heart, adipose tissue, and foregut but increased it in the hindgut and white muscle. Similarly, ampkα2 expression decreased in the hypothalamus and muscle during fasting, with an increase in the midgut. Glucose tolerance tests showed dynamic regulation of ampkα1 and ampkα2 , with initial downregulation followed by upregulation in the hepatopancreas, red muscle, and brain. High-glucose and high-fat diets significantly increased ampkα1 and ampkα2 expression in multiple tissues. Insulin and glucagon treatment induced time-dependent changes in both genes in hepatocytes, while Aeromonas hydrophila infection, LPS, and Poly (I:C) stimulation upregulated ampkα1 and ampkα2 in immune-related tissues. Knockdown of ampkα2 , but not ampkα1 , reduced glut1b mRNA levels, while both knockdowns of ampkα1 and ampkα2 promoted the expression of gsk3β , pygm , acc , fas , srebp , cs , and pro-inflammatory cytokines, suggesting their involvement in metabolic and immune regulation in carp.
研究领域:
分子生物学
·
营养学
·
免疫学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BULLETIN OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY
·
2013
·Vol. 91
·期 5
·522-525
·
IF 1.105
Li Yong-Fang,Yang Zhong-Zhi,Fan Jing,Zhu Yong
Five higher chlorinated benzenes (including hexachlorobenzene (HCB), pentachlorobenzene (PeCB) and three isomers of tetrachlorobenzens) were measured in the livers of grass carp and common carp collected from five markets in Xinxiang city, China. HCB and PeCB were detected in all samples. The major component of the higher CBs residue was HCB and significant correlations existed between HCB and PeCB in both grass carp and common carp livers. The ratio range of HCB/PeCB in grass carp and common carp were 3.4–6.2 and 4.9–7.7, respectively, which implied the sources of higher CBs originate mainly from the revolatilization of industrial HCB with a minor impact from PeCB.
研究领域:
环境科学
·
毒理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
WATER AIR AND SOIL POLLUTION
·
2021
·Vol. 232
·期 9
·1-21
·
IF 2.52
Cano-Viveros Selene,Galar-Martínez Marcela,Gasca-Pérez Eloy,García-Medina Sandra,Ruiz-Lara Karina,Gómez-Oliván Leobardo Manuel,Islas-Flores Hariz
Aluminum, mercury, and iron have been found in high concentrations in various freshwater bodies around the world and have been shown to be very harmful contaminants to hydrobionts, causing metabolic dysfunction and damage at various levels. However, studies on mixtures of these pollutants are scarce, particularly in younger or developing organisms, which are more sensitive to damage. Therefore, the objective of this work was to evaluate the toxicity of Al, Fe, and Hg, in isolation and in mixture, on common carp embryos exposed to the maximum permissible limits described in the Mexican regulations for the protection of aquatic life, correlating the biomarkers of oxidative stress with the effects on embryonic development. For this purpose, the Cyprinus carpio embryos were exposed to iron (0.1 mg L −1 ), mercury (0.00001 mg L −1 ), aluminum (0.05 mg L −1 ), and their mixture, throughout their development and until hatching. The activity of the antioxidant enzymes (SOD, CAT, and GPx), the degree of lipoperoxidation, and the content of hydroperoxides and total proteins, as well as the morphological development of the embryos were evaluated at 12 h, 24 h, 48 h, 72 h, and 96 h of exposure. The results showed that the metals under study are toxic to C. carpio embryos and that their interaction modifies the toxic response. Thus, iron generates alterations in the activity of antioxidant enzymes and modification in embryonic development to a lesser extent than aluminum and the mixture of metals, while mercury exerts its toxicity on embryos by mechanisms other than oxidative stress, but the modification to the activity of antioxidant enzymes may contribute to the changes observed in embryonic development. The changes in the response of biomarkers of embryotoxicity and oxidative stress suggest that the combination of metals produces an antagonism-type interaction, so this study provides a precedent for future research to determine the type of interaction that a mixture of contaminants generates in developing aquatic organisms. Graphical abstract
研究领域:
环境毒理学
·
水产生物学
·
生态学
✓ 已验证
DOI
高影响因子
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2022
·Vol. 23
·期 11
·6282
·
IF 6.208
Weikai Ding,Yousef Sultan,Shumei Li,Wenjun Wen,Bangjun Zhang,Yiyi Feng,Junguo Ma,Xiaoyu Li
Previous studies have indicated that the harmful heavy metal lead (Pb) contamination in aquatic systems has caused intelligence development disorders and nervous system function abnormalities in juveniles due to the increased permeability of the blood–brain barrier. Ionic liquids (ILs) are considered “green” organic solvents that can replace traditional organic solvents. Studies have found the presence of ILs in soil and water due to chemical applications or unintentional leakage. Therefore, what would happen if Pb interacted with ILs in a body of water? Could ILs enable Pb to more easily cross the blood–brain barrier? Therefore, we examined the combined exposure of Pb and ILs in common carp at low concentration (18.3 mg L−1of Pb(CH3COO)2•3 H2O and 11 mg L−1of the IL 1-methyl-3-octylimidazolium chloride, 5% of their LC50) for 28 days in the present study. The result of a neurobehavioral assay showed that chronic exposure of lead at lower concentrations significantly altered fish movement and neurobehaviors, indicating that lead exposure caused neurotoxicity in the carp. Increases in the neurotransmitter dopamine levels and injuries in the fish brain accounted for neurobehavioral abnormalities induced by lead exposure. Moreover, we also found that lead could easily cross the blood–brain barrier and caused significant bioaccumulation in the brain. Particularly, our study indicated that the ionic liquid could not synergistically promote blood–brain barrier permeability and hence failed to increase the absorption of lead in the fish brain, suggesting that the combined exposure of lead and ILs was not a synergistic effect but antagonism to the neurotoxicity. The results of this study suggested that ILs could recede the Pb induced neurotoxicity in fish.Keywords:Cyprinus carpio;Pb;ionic liquid;combined exposure;neurotoxicity
研究领域:
水生毒理学
·
环境科学
·
神经科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMOSPHERE
·
2023
·
IF 8.8
Lu Qiao,Lu Chen,Zhen Yang,Jinhua Xu,Jinlong Song,Xingyang Li,Huiwu Sun,Yingchun Mu
Diflubenzuron has been applied in agriculture and aquaculture, and its residues in ecological environment and food chain could result in chronic exposure and long-term toxicity effects for human health. However, limited information is available regarding diflubenzuron levels in fish and associated risk assessment. This study performed the analysis for dynamic bioaccumulation and elimination distribution of diflubenzuron in carp tissues. The results indicated that diflubenzuron was absorbed and enriched by fish body along with higher enrichment in lipid-rich tissues of fish. The peak concentration in carp muscle reached 6-fold of diflubenzuron concentration in aquaculture water. The median lethal concentration (LC 50 ) of diflubenzuron at 96 h was 12.29 mg/L, presented low toxicity to carp. Risk assessment results showed that the chronic risk from dietary exposure to diflubenzuron through carp consumption for Chinese residents of children and adolescents, adults and elderly people were acceptable, while posed a certain risk for young children . This study provided the reference for pollution control , risk assessment and scientific management of diflubenzuron.
研究领域:
环境科学
·
毒理学
·
水产养殖学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
·
2022
·
IF 4.966
Tianmeng Zhang,Zhuhua Dong,Feixue Liu,Enzhuang Pan,Nana He,Fenfen Ma,Xinyu Wu,Yan Wang,Jingquan Dong
Avermectin is one of the most widely used pesticides, but its toxicity to non-target organisms, especially aquatic organisms , has been ignored. Therefore, an acute spleen injury model of avermectin in carp was established to assess the non-target toxicity of avermectin to carp. In this study, 3.005 μg/L and 12.02 μg/L were set as the low and high dose groups of avermectin, respectively, and a four days acute exposure experiment was conducted. Pathological structure observation showed that avermectin damaged spleen tissue structure and produced inflammatory cell infiltration . Biochemical analysis showed that avermectin significantly reduced the activities of antioxidant enzymes CAT, SOD, and GSH-px, but increased the content of MDA, a marker of oxidative damage. Avermectin exposure also significantly increased the transcription levels of inflammatory cytokines such as IL-1β, IL-6, TNF-α, and INOS, and also significantly enhanced the activity of the inflammatory mediator iNOS, but suppressed the transcription levels of anti-inflammatory factors TGF-β1 and IL-10. In addition, TUNEL detected that the apoptosis rate increased significantly with the increase of avermectin dosage, and the transcription levels of apoptosis-related genes BAX, P53, and Caspase 3/9 also increased in a dose-dependent manner. This study is preliminary evidence that avermectin induces spleen injury in carp through oxidative stress , inflammation, and apoptosis, which has important implications for subsequent studies on the effects of avermectin on non-target organisms.
研究领域:
水生生物学
·
毒理学
·
环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
·
2024
·
IF 4.2
Hongmin Lu,Yue Zhang,Xin Zhang,Ruoqi Wang,Tiantian Guo,Qi Wang,Hongjing Zhao,Mingwei Xing
Environmental pollution caused by arsenic or its compounds is called arsenic pollution. Arsenic pollution mainly comes from people mining and smelting arsenic compounds. In addition, arsenic compounds' widespread use and production of arsenic-containing pesticides, arsenic-rich water used to irrigate farms, or high arsenic levels in foods caused by coal burning are all sources of arsenic contamination. Arsenic contamination poses a significant threat to global public health. It is reported that exposure to arsenic can induce severe renal injury. However, the underlying mechanism needs to be clarified. In this study, the arsenic exposure model in vivo and in vitro was used to explore the mechanism of arsenic-induced renal injury, especially the role of ferroptosis and its regulatory mechanism, and then to evaluate its anti-pollution effect by supplementing zinc. The results showed that arsenic significantly induced ferroptosis, characterized by up-regulating the expression of YAP and TFR in kidney and CIK cells and then increasing the levels of Fe 2+ and ROS, lipid peroxidation, and iron metabolism. Microscopic observation revealed the shrinkage of mitochondria and the increase in membrane density. In addition, molecular docking and inhibitor experiments further confirmed that arsenic is involved in the process of ferroptosis by activating YAP and TFR. These results clarify the harmful effects of arsenic on carp kidneys and its mechanism and highlight the critical interactions between the YAP-TFR pathway, ROS, and ferroptosis. Importantly, this study found that zinc can reduce ferroptosis caused by the arsenic-activated YAP-TFR pathway by inhibiting YAP activation and lipid peroxidation.
研究领域:
环境科学
·
毒理学
·
水生生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
AQUACULTURE
·
2024
·
IF 3.9
Tian-Xiu Qiu,Lei Liu,Xu Zhang,Yang Hu,Jiong Chen
Isoliquiritigenin, a flavonoid from Glycyrrhiza glabra , demonstrates potent antiviral properties by enhancing host defenses through antioxidative and immunomodulatory mechanisms. Our in vitro data demonstrate that isoliquiritigenin significantly inhibits spring viremia of carp virus (SVCV) at concentrations up to 100 μg/ml without inducing cytotoxicity, effectively reducing viral replication and the production of new viral particles. Treatment with isoliquiritigenin markedly reduced the cytopathic effects in EPC cells, improving cell viability by more than 50 %. Furthermore, isoliquiritigenin enhances resistance to SVCV infection by significantly reducing intracellular the viral load when cells are pretreated for up to 24 h. This inhibition primarily occurs during the viral replication and assembly phases, without affecting viral binding or entry. Additionally, isoliquiritigenin strengthens antioxidant defenses and modulates immune responses, thereby reducing oxidative damage and interferon production. In vivo experiments with juvenile carp corroborate the antiviral efficacy of isoliquiritigenin, showing significantly reduced mortality rates and the viral load in SVCV-infected fish. These findings highlight the potential of isoliquiritigenin as an antiviral agent in aquaculture, providing a sustainable alternative to traditional chemotherapeutics by concurrently inhibiting viral replication and enhancing host immune defenses.
研究领域:
水产养殖学
·
病毒学
·
免疫学
✓ 已验证
产品关联
DOI
高影响因子
Animals
·
2025
·Vol. 15
·期 2
·140
·
IF 2.7
Kexin Ma,Yueyao Yang,Yifan Li,Chuan Li,Taicheng Li,Haiyan Ma,Zibin Jiang,He Zhou,Wei Wang
Simple SummaryOur study investigates the karyotype of a vital polyploid fish,Carassius auratus gibelio. We found 150 chromosomes with 6 nucleolar organizer regions, suggesting complex chromosomal interactions. Male and femaleC. gibelioshow different chromosome pairings, indicating asynchronous evolution. The formation of two trivalents or two bivalents and two univalents from six NOR bearing chromosomes in maleC. gibeliois considered evidence of being in the process of rediploidization. The presence of only univalents and bivalents in the oocytes of femaleC. gibelioindicates that their cytogenetics are more stable, suggesting that they have completed rediploidization. Our findings contribute to the understanding of the underlying mechanisms and evolutionary outcomes of this species’ genome. They provide relevant insights for further exploration of the reproductive modes of polyploid organisms and breeding strategies.Carassius auratus gibelio, a significant polyploid fish in Chinese aquaculture, can reproduce through both unisexual gynogenesis and sexual reproduction. WhileC. gibeliois known to exhibit polyploidy throughout its lifecycle, whether the homologous pairing of chromosomes is associated with the mode of reproduction remains unclear. Our study determined its karyotype as 2n = 150, comprising 42 metacentric, 24 submetacentric, 6 subtelocentric, and 78 telocentric chromosomes. Using AgNORs, CMA3/DAPI double fluorescence staining, and FISH, we identified six nucleolar organizer regions inC. gibeliochromosomes. FemaleC. gibelioexhibited only univalent and bivalent configurations, while males showed a range of chromosome configurations from univalent to hexavalent. CMA3-positive sites and FISH signals in trivalents spermatocytes hint at homologous triploidization. The formation of two trivalents or two bivalents and two univalents from six NORs bearing chromosomes in maleC. gibeliois considered evidence of being in the process of rediploidization. The presence of only univalents and bivalents in the oocytes of femaleC. gibelioindicates that their cytogenetics are more stable, suggesting that they have completed rediploidization. Asynchronous progression of polyploidization may lead to differences in reproductive patterns between male and femaleC. gibelio. Our results offer cytogenetic insights into the polyploidy and evolution ofC. gibelio, providing foundational cytogenomics data. This study provides relevant references for revealing the distinct reproductive strategies of male and femaleC. gibelio: under stable conditions, gynogenesis enables rapid clonal reproduction and swift population growth, while sexual reproduction enhances the genetic diversity within the population.
研究领域:
细胞遗传学
·
进化生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Fishes
·
2025
·Vol. 10
·期 6
·248
·
IF 2.4
Qianxue Shao,Yongming Ruan,Ru Liang,Ruixin Jin,Zhixi Jin,Lin Xie,Yongqing Chi,Jiaojiao Xia,Pingyang Zhu
Integrated rice–fish farming, crucial for sustainable agriculture, relies on the judicious use of pesticide. This study evaluates the toxicity of six common rice-field pesticides onProcypris merus(rice flower carp), a key species in these systems. We conducted acute and chronic toxicity tests, assessing survival, growth, oxidative stress (SOD, CAT, MDA, 8-OHdG), and neurotoxicity (AChE). Results revealed a spectrum of toxicity: abamectin and trifloxystrobin were highly toxic; pretilachlor was moderately so; and glufosinate-ammonium, triflumezopyrim, and thiazole zinc were low. Notably, triflumezopyrim induced significant oxidative stress and DNA damage, while all three low-toxicity pesticides inhibited AChE activity, indicating potential neurotoxicity. Despite these effects, all observed toxicities were reversible within 7–14 days. Considering that the tested concentrations exceeded typical field application rates, glufosinate-ammonium, triflumezopyrim, and thiazole zinc are deemed relatively safe forP. merusat recommended dosages. Our findings provide critical insights for optimizing pesticide selection in rice–fish farming, balancing pest control with ecological safety, thereby informing sustainable agricultural practices.
研究领域:
生态毒理学
·
水产养殖
·
农业科学
✓ 已验证
产品关联
DOI
高影响因子
AQUACULTURE INTERNATIONAL
·
2025
·Vol. 33
·期 1
·1-18
·
IF 2.4
Sha Yuanpu,Chen Yuhuan,Dong Jing,Gao Xiaofei,Yuan Huatao,Zhang Jingxiao,Gao Yunni,Li Xuejun
This study aims to verify the effects of astaxanthin microcapsules on the growth, physiology, and nutritional value of Cyprinus carpio ( C. carpio ), and evaluate the feasibility and safety of microcapsule technology in aquaculture. In this experiment, natural and synthetic astaxanthin were encapsulated in microcapsules, with empty microcapsules used as a control, and C. carpio was fed these diets for 8 weeks. Growth, muscle quality, and the antioxidant system were then assessed. The results showed that (1) the weight gain rate (WGR), specific growth rate (SGR), and condition factor (K) in group A (natural astaxanthin) and group B (synthetic astaxanthin) were significantly higher than those in the control group, with no significant differences in viscerosomatic index (VSI) or hepatosomatic index (HSI) across the groups. (2) Astaxanthin supplementation enhanced the antioxidant activity in the hepatopancreas of C. carpio . Total superoxide dismutase (T-SOD) activity significantly increased, malondialdehyde (MDA) levels decreased, while no significant changes were observed in total antioxidant capacity (T-AOC) or catalase (CAT) activity. The sod1 and gpx1a genes were significantly upregulated in group A. (3) Astaxanthin also improved muscle nutritional quality by significantly increasing crude protein content, reducing crude ash content, and enhancing water-holding capacity. Amino acid and fatty acid composition also showed improvement. Overall, this study suggests that natural astaxanthin may be superior to synthetic astaxanthin, and provides preliminary evidence that self-made astaxanthin microcapsules are safe for future aquaculture, offering a scientific basis for sustainable and eco-friendly aquaculture practices.
研究领域:
水产养殖学
·
营养学
·
分子生物学
✓ 已验证
产品关联
DOI
高影响因子
AQUATIC TOXICOLOGY
·
2025
·
IF 4.3
Wanglong Zhang,Yanjiao Zong,Ruize Sun,Zhenhong Xue,Wenhui Wan,Anran Ren,Yongchao Ma,Wenjing Tian,Renjun Wang
The global water pollution now calls for precise risk assessment of chemicals, e.g., dioxins and the dioxin-like compounds (DLCs). The freshwater and marine medaka have been widely implemented in the toxicity testing, and perhaps give mechanistic information for comparative biology. The question that ‘will they report equal results due to their close phylogenetic relation’ has been raised, therefore, we explored their physiological and molecular responses to dioxin. As the mediator of the dioxin toxicity, the aryl hydrocarbon receptor (AHR) of marine medaka ( Oryzias melastigma ) has not been functionally characterized and might be species-specific. In terms of sensitivity to dioxin—2,3,7,8-tetrachlorodibenzo- p -dioxin (TCDD), the EC 50 values of omeAHR1a (0.16±0.12 nM), omeAHR1b (2.96±2.96 nM), omeAHR2a (0.44±0.30 nM), and omeAHR2b (9.00±6.88 nM) exhibit marked variations. The omeAHR2a and omeAHR1a display heightened sensitivity compared to the freshwater Japanese medaka ( Oryzias latipes ) counterparts olaAHR2a and olaAHR1a, respectively. The results indicate the in vitro sensitivity of AHR among species can vary by one or two orders of magnitude. Further mechanistic investigations using additional ligands and computational modeling reveal that: 1) most of omeAHR2a, olaAHR2a, dreAHR2, and hsaAHR interact with ligands in the affinity order of TCDD > PCB126 > BNF > indole, mirroring their AHR transactivation potency, but the docking poses and dynamics can vary; 2) one AHR subform's high sensitivity to dioxin—TCDD may extend to DLCs but not to other types of ligands. Beyond the in vitro study, the preliminary in vivo LC 50 data indicate that marine medaka (LC 50 : 1.64 ng/L (95% CI: 1.05-2.55 ng/L)) has similar sensitivity, and possibly slightly greater (not statistically determined yet), to TCDD in comparison with Japanese medaka (LC 50 : 3.42 ng/L (95% CI: 1.37-6.48 ng/L)).These insights underscore the difference of AHR biology among species even the close relative species, and point out the necessity for meticulous consideration when evaluating the toxicity of compounds and when extending predictive toxicity assessments to more species.
研究领域:
环境科学
·
分子生物学
·
毒理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）