FOOD CHEMISTRY
·
2024
·
IF 8.5
Tao Gou,Wenxiu Li,Shenglin Chen,Chao Yi,Yu Guo,Zheng Cao,Lei Zhou,KangJu Lee,Mingrui Chen,Yaowen Liu
In this study, a hydrophobic and antibacterial pad was prepared to preserve Channel Catfish ( Ictalurus punctatus ). The pad composite the microfibrillated cellulose and β-cyclodextrin/nisin microcapsules. The hydrophobic pad ensures a dry surface in contact with the fish, reducing microbial contamination. The pad has a low density and high porosity, making it lightweight and suitable for packaging applications, while also providing a large surface area for antibacterial activity. Results demonstrated that this antibacterial pad exhibits an ultralow density of 9.0 mg/cm 3 and an ultrahigh porosity of 99.10%. It can extend the shelf life of Channel Catfish fillets to 9 days at 4 °C, with a total volatile base nitrogen below 20 mg/100 g. The study proposes a novel solution for preserving aquatic products by combining antibacterial substances with the natural base material aerogel. This approach also extends the utilization of aerogel and nisin in food packaging.
研究领域:
食品科学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Food Chemistry-X
·
2025
·
IF 8.2
Mingzhu Zhou,Yiting Lu,Jinyu Yu,Chao Wang,Wei Yu,Liu Shi,Wenjin Wu,Lan Wang,Yu Qiao
Gas chromatography-electronic nose (GC-E-Nose), gc-ion mobility spectrometry (GC-IMS), and gc-olfactory-mass spectrometry (GC-O-MS) techniques, along with odor recombination and omission experiments, were employed to characterize the off-flavors in commercially available ready-to-heat roasted catfish after reheating. A total of 19 (DR), 11 (HR), and 21 (YR) characteristic compounds were identified across the three samples. The 19 characteristic compounds from the DR group were reconstituted to develop recombination and omission models. The resulting model demonstrated the highest similarity (84.98 %) to the original sample while retaining some odor information from the other two sample groups. The 12 key off-flavor substances were identified through the omission model and were subsequently subjected to multivariate recombined. The contribution of these off-flavors was further validated via omission testing. The results indicated that the primary off-flavors in reheated ready-to-heat roasted catfish were attributed to hexanal, heptanal, (E)-2-hexenal, octanal, 3-methyl-1-butanol, 1-octen-3-ol, and (E,E)-2,4-heptadienal. This characterization of off-flavors in ready-to-heat roasted catfish post-reheating will contribute to the regulation of the product's flavor quality.
研究领域:
食品科学
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分析化学
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感官评价
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食品加工与贮藏
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风味化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
AQUACULTURE
·
2019
·
IF 3.022
Jian Li,Xiaojun Xie
This study investigated the effect of waterborne cadmium (Cd) exposure on the growth performance, specific dynamic action (SDA), and energy budget of southern catfish ( Silurus meridionalis ). S. meridionalis was exposed to various Cd concentrations (0–500 μg L −1 ) for 8 weeks. The specific growth rate (SGR), apparent digestibility, protein apparent digestibility, SDA parameters, and components of energy budgets were then determined. Compared to the control group, SGR was significantly reduced upon Cd exposure at 250 μg L −1 and 500 μg L −1 . Compared to the control group, the experimental groups (62.5–500 μg L −1 ) showed significantly lower total feed intake and feed conversion efficiency. The apparent digestibility and protein apparent digestibility was significantly decreased in the 250 μg L −1 and 500 μg L −1 experimental groups compared to the control group. A significantly lower peak metabolic rate, SDA, and SDA coefficient were observed in the 250 μg L −1 and 500 μg L −1 experiment groups compared to the control group. The ratio of growth energy to assimilation energy in the 62.5–500 μg L −1 experimental groups was significantly lower than that of the control group. There was a significant increase in the ratio of metabolic energy to assimilation energy in Cd exposed groups compared to the control group. These results indicate that waterborne Cd exposure decreased the protein apparent digestibility, resulting in decreased SDA and SDA coefficient. Waterborne Cd exposure resulted in increased proportion of excretion energy and feces energy in S. meridionalis , whereas assimilation energy was reduced. Moreover, for assimilation energy, the energetic proportion for metabolism was increased, whereas the energetic proportion for growth was reduced
研究领域:
水生毒理学
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鱼类生理学
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环境科学
✓ 已验证
产品关联
DOI
高影响因子
FISH & SHELLFISH IMMUNOLOGY
·
2018
·
IF 3.185
Liting Wu,Shengli Fu,Xiaoxue Yin,Wenna Leng,Zheng Guo,Anli Wang,Jianmin Ye
Affinity maturation of the antibody response , a process of antibody affinity increasing over response, is one of the key features of the mammalian immune system. However, the process is incompletely understood in teleost, including channel catfish ( Ictalurus punctaus ). In this study, IgM affinity maturation in channel catfish was investigated by estimating the kinetics of antibody affinity using ELISA and ELISPOT assays . Fish were immunized with a T-cell dependent antigen (TNP-KLH), and individual serum IgM antibody titers and affinities, and IgM + antibody-secreting cells (ASCs) in peripheral blood were analyzed over a period of 14 weeks. A detectable serum anti-TNP response developed by 2-weeks post-immunization, and the maximal antibody production was observed by 6-weeks post-immunization. The average affinity of anti-TNP serum antibody increased consistently and reached the maximum by 10-weeks post-immunization. The increase of antibody affinity beyond the point of optimal antibody titer revealed that the affinity maturation of IgM antibody response occurred in channel catfish. Dissection of dynamics of individual affinity subpopulations indicated that a significant proportion of low affinity subpopulations appeared at early response, and high affinity subpopulations appeared predominantly at later, resulting in a 100-fold increase in affinity over response. Additional, TNP + IgM + ASCs was detected by 2-weeks post-immunization and achieved the maximal number by 6-weeks post-immunization. Using an inhibition ELISPOT assay, the findings of a consistent increase in the average affinity of secreted IgM antibody by peripheral blood ASCs, as the immune response progressed, confirmed the occurrence of the affinity maturation. Taken together, the results of this study indicated that affinity maturation occurred in channel catfish following immunization with a TD antigen TNP-KLH.
研究领域:
免疫学
·
鱼类生物学
✓ 已验证
DOI
PubMed
高影响因子
ELECTROANALYSIS
·
2023
·Vol. 36
·期 2
·e202300237
·
IF 3
Toleshi Teshome,Shimeles Addisu Kitte,Abera Gure,Guta Gonfa
The co-precipitation approach was employed in this study to create Fe doped ZnO (Fe-ZnO) nanoparticles (NPs). To characterize the synthesized NPs, Ultra violet-Visible (UV-Vis) spectroscopy, X-ray diffraction (XRD), fourier transform infrared (FT-IR), scanning electron microscopy (SEM), and Brunauer-Emmett-Teller (BET) were used. Here, a novel electrochemical approach for the detection of tryptophan (Trp) using a glassy carbon electrode modified with Fe-ZnO NPs (Fe-ZnO/GCE) is demonstrated. The research findings revealed that Fe-ZnO/GCE enhanced the electron transfer rate of Trp oxidation. The sensitivity of Trp detection using Fe-ZnO/GCE in phosphate buffer solution (PBS) at pH 64.0 was significantly improved compared to bare GCE due to the electrocatalytic activity of Fe-ZnO NPs. Fe-ZnO/GCE exhibited a linear response with Trp concentrations ranging from 0.1 to 150 μM with the limit of detection (LOD) is 0.089 μM (3σ /m ) and limit of quantification (LOQ) about 0.3 μM (10σ /m ) using linear sweep voltammetry (LSV). The Fe-ZnO/GCE sensor was also effectively used to detect Trp in African catfish and multivitamin tablets. Trp analysis in real samples exhibited good recovery values of 89.60 to 99.15 %, demonstrating the accuracy and practicality of the method.
研究领域:
分析化学
·
生物医学工程
·
材料科学
✓ 已验证
DOI
高影响因子
FOOD MICROBIOLOGY
·
2024
·
IF 4.5
Yali Li,Tianqi Wangjiang,Zhida Sun,Liu Shi,Sheng Chen,Lang Chen,Xiaojia Guo,Wenjin Wu,Guangquan Xiong,Lan Wang
Aeromonas veronii is associated with food spoilage and some human diseases, such as diarrhea, gastroenteritis, hemorrhagic septicemia or asymptomatic and even death. This research investigated the mechanism of the growth, biofilm formation, virulence, stress resistance, and spoilage potential of Bacillus subtilis lipopeptide against Aeromonas veronii . Lipopeptides suppressed the transmembrane transport of Aeromonas veronii by changing the cell membrane's permeability, the structure of membrane proteins, and Na + /K + -ATPase. Lipopeptide significantly reduced the activities of succinate dehydrogenase (SDH) and malate dehydrogenase (MDH) by 86.03% and 56.12%, respectively, ultimately slowing Aeromonas veronii growth. Lipopeptides also restrained biofilm formation by inhibiting Aeromonas veronii motivation and extracellular polysaccharide secretion. Lipopeptides downregulated gene transcriptional levels related to the virulence and stress tolerance of Aeromonas veronii . Furthermore, lipopeptides treatment resulted in a considerable decrease in the extracellular protease activity of Aeromonas veronii , which restrained the decomposing of channel catfish flesh. This research provides new insights into lipopeptides for controlling Aeromonas veronii and improving food safety.
研究领域:
微生物学
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食品科学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2017
·Vol. 184
·期 9
·3557-3563
·
IF 4.58
Liu Xiuying,Gao Tengfei,Gao Xue,Ma Tao,Tang Yiwei,Zhu Lijie,Li Jianrong
The authors describe an aptamer based method for the determination of the antibiotic sulfadimethoxine (SLF). It is making use of upconversion nanoparticles (UCNPs) that act as an internal fluorophors signals, and of aptamers that can specifically recognize SLF. The UCNPs were deposited on the surface of magnetic nanoparticles (magNPs) functionalized with the aptamer. In the presence of SLF, parts of the UCNPs dissociate from the surface of the magNPs, and this results in decreased fluorescence intensity. The findings were exploited to design a fluorometric assay for SLF that has a 0.11 ng·mL −1 detection limit. The method was successfully applied to the quantification of SLF in spiked samples of perch and catfish. Recoveries range from 80.3% to 92.7%, and RSDs from 2.4% to 3.6%.
Schematic of an aptamer based method for the determination of the antibiotic sulfadimethoxine (SLF). It is making use of upconversion nanoparticles (UCNPs) that act as an internal fluorophors signals, and of aptamers that can specifically recognize SLF.
研究领域:
分析化学
·
纳米技术
·
环境科学
·
药物分析
✓ 已验证
DOI
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Jie Li,Zhi Li,Yanli Dong,Qianqian Wang,Shuofeng Li,Zhi Wang,Chun Wang,Qiuhua Wu
In this paper, a novel magnetic hyper-crosslinked polymer with amino and triazine bifunctional groups (M-NH 2 -THCP) was developed. M-NH 2 -THCP has strong nitroimidazoles (NDZs) enrichment effect, and therefore it was used as an adsorbent to extract five NDZs from lake water, catfish and shrimp meat prior to HPLC. Polar interaction, π-π stacking interaction, hydrogen bond and Lewis acid-base interaction were attested to be the major adsorption mechanism. The method has a good linearity in the range of 0.1–100 ng mL −1 for lake water, 10–400 ng g −1 for catfish and shrimp muscle with R 2 > 0.9964. The limits of detection of NDZs were 0.03–0.04 ng mL −1 for lake water, 1.0–2.0 ng g −1 for catfish and 2.0–2.5 ng g −1 for shrimp, which is superior to most reported method. The method recoveries were 87.6–119 %, and relative standard deviations were less than 8.7 %. M-NH 2 -THCP holds great application potential in pollutants enrichment, separation and removal.
研究领域:
环境科学
·
分析化学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）