Toxics
·
2023
·Vol. 11
·期 9
·743
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IF 4.6
Hao Wu,Xiping Yuan,Jinwei Gao,Min Xie,Xing Tian,Zhenzhen Xiong,Rui Song,Zhonggui Xie,Dongsheng Ou
Current treatment strategies for parasitic infectious diseases in crucian carp primarily rely on chemotherapy. As a commonly used antiparasitic agent, deltamethrin (DEL) may have the potential adverse effects on external mucosa of fish such as gills. In this study, 180 healthy juvenile crucian carp (Carassius auratus) (average weight: 8.8 ± 1.0 g) were randomly divided into three groups for 28 days, which were immersed in 0 μg/L, 0.3 μg/L, and 0.6 μg/L of DEL, respectively. The results of histological analysis revealed that severe hyperplasia in the secondary lamellae of gills was observed, and the number of goblet (mucus-secreting) cells increased significantly after DEL immersion. TUNEL staining indicated that the number of apoptotic cells increased in crucian carp gill. At the molecular level, the mRNA expression analysis revealed significant upregulation of apoptosis (caspase 3, caspase 8, and bax), autophagy (atg5 and beclin-1), and immune response (lzm, muc5, il-6, il-8, il-10, tnfα, ifnγ, tgfβ, tlr4, myd88, and nf-kb), whereas tight junction-related genes (occludinandclaudin12) were downregulated after DEL immersion, suggesting that DEL immersion altered innate immunity responses and promoted mucus secretion. Moreover, tandem mass tag (TMT)-based proteomics revealed that a total of 428 differentially expressed proteins (DEPs) contained 341 upregulated DEPs and 87 downregulated DEPs with function annotation were identified between the control and DEL groups. Functional analyses revealed that the DEPs were enriched in apoptotic process, phagosome, and lysosome pathways. Additionally, DEL immersion also drove gill microbiota to dysbiosis and an increase in potentially harmful bacteria such asFlavobacterium. Overall, this study showed that DEL elicited shifts in the immune response and changes in the surface microbiota of fish. These results provide new perspectives on the conventional anthelmintic concentration of DEL immersion disorder of the gill immune microenvironment in crucian carp and theoretical support for future optimization of their practical application.Keywords:deltamethrin;gill barrier;TMT-based proteomics;gill microbiota;cell apoptosis
研究领域:
水产养殖
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免疫学
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蛋白质组学
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微生物学
✓ 已验证
DOI
PubMed
高影响因子
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2017
·Vol. 24
·期 10
·9379-9386
·
IF 2.741
Jia Yuyu,Wang Lin,Qu Zhipeng,Wang Chaoyi,Yang Zhaoguang
Three fish species ( Carassius auratus , Pelteobagrus fulvidraco , and Squaliobarbus curriculus ) were collected from Xiang River near Changsha City, Southern China. The concentrations of heavy metals including arsenic (As), cadmium (Cd), copper (Cu), iron (Fe), manganese (Mn), lead (Pb), and zinc (Zn) in the muscle, gill, and liver of three species were determined by the inductively coupled plasma mass spectrometry method. One-way analysis of variance (ANOVA) was introduced to determine the significant variations ( p < 0.05) of heavy metals. Livers were found to accumulate Cd and Cu due to the metallothionein proteins. High levels of Mn and Pb in the gills indicated that the main uptake pathway of these heavy metals was from the water. The carnivorous species, P. fulvidraco , was found to accumulate the highest levels of toxic elements (As, Cd, and Pb), while relatively high concentrations of nutrient elements (Cu, Fe, Mn, and Zn) were accumulated in omnivorous species ( C. auratus and S. curriculus ). According to the results of Pearson’s correlation analysis, there were few significant relationships at p < 0.01 level between the concentrations of the analyzed elements and the fish sizes. The results of risk assessment indicated that exposure to the toxic heavy metals from fish muscle consumption posed no non-carcinogenic health risk to local inhabitants.
研究领域:
生态毒理学
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环境科学
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产品关联
DOI
PubMed
高影响因子
APPLIED CATALYSIS B-ENVIRONMENTAL
·
2017
·
IF 9.446
Wenlong Zhen,Xiaofeng Ning,Baojun Yang,Yuqi Wu,Zhen Li,Gongxuan Lu
CdS photocorrosion seriously impeded its application in photocatalysis , especially for water splitting. Here we report new strategies to improve CdS photocorrosion resistance properties significantly by coating Ni 2 P shell and assembling an artificial gill to remove newly formed O 2 from water. Ni 2 P@CdS catalyst can achieve the over-all water splitting under visible light irradiation without addition of any sacrifice reagent and noble metal loading. Compared with CdS itself, the 10Ni 2 P@CdS photocatalyst exhibits excellent photocatalytic activity for hydrogen evolution (251.4 μmol of H 2 in 180 min) with a high AQE (3.89% at 430 nm). This catalyst also presents high photocurrent, low overpotential (−0.32 V vs SCE), and long fluorescence lifetime (16.27 ns) of excited charges. Cd 2+ ions concentration measured by ICP and long term stability results verified the anti-photocorrosion role of Ni 2 P shell on CdS during water splitting reaction. The activity and stability of 10Ni 2 P@CdS is even superior to typical 1Pt@CdS catalyst. Our results confirm CdS can be an active catalyst for photocatalytic hydrogen generation from water under visible irradiation if its stability is enhanced by protection of anti-photocorrosion over-coating shell and removing the nascent formed oxygen from water.
研究领域:
材料科学
·
化学
✓ 已验证
产品关联
DOI
高影响因子
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
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2022
·
IF 4.52
Rui Zhang,Zhigang Zhao,Mingshuai Li,Liang Luo,Shihui Wang,Kun Guo,Wei Xu
Aquatic water with carbonate alkalinity presents a survival challenge to aquatic animals. As an economically important crab, large quantities of Eriocheir sinensis are cultured in carbonate-type saline-alkali ponds, while the toxic effect on E. sinensis from carbonate alkalinity is still unclear. In this study, untargeted liquid chromatography-mass spectrometry metabolomics was performed to investigate the metabolic change caused by culture alkalinity, and confirmed distinct physiological response under gradient alkalinities. There were 39 differential metabolites obtained in the low-alkalinity group (4.35 mmol/L) versus control group, and “arachidonic acid metabolism” was enriched as a core response pathway. 93 differential metabolites were identified in the high-alkalinity group (17.43 mmol/L) versus control group, and a complex response net was manifested through integrated analysis, building by “steroid hormone biosynthesis”, “phenylalanine, tyrosine and tryptophan biosynthesis”, “phosphonate and phosphinate metabolism”, “phenylalanine metabolism”, “mineral absorption”, “purine metabolism” and “carbon metabolism”. This indicated the mobilization of energy reserves and the suppression of protein and amino acid catabolism were manifested in E. sinensis gills to defense high alkalinity stress. In addition, the persistently regulation of key metabolites under various alkalinity, including diuretic compound “spironolactone” and the antiphlogistic compound “LXB 4 ”, suggested anti-inflammatory action and excretion regulation were initiated to defend the stress.
研究领域:
水产养殖学
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环境毒理学
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代谢组学
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产品关联
DOI
PubMed
高影响因子
AQUATIC TOXICOLOGY
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2014
·
IF 3.513
Tao Wang,Xiaohua Long,Yongzhou Cheng,Zhaopu Liu,Shaohua Yan
Copper nanoparticles (Cu-NPs) were widely used in various industrial and commercial applications. In this study the effects of Cu-NPs and soluble Cu were investigated on juvenile Epinephelus coioides . The fish were exposed in triplicate to control, 20 or 100 μg Cu L −1 as either copper sulphate (CuSO 4 ) or Cu-NPs in a semi-static aqueous culture for 25 days. The growth parameters were significantly lower at 100 μg Cu L −1 as CuSO 4 or Cu-NPs treatment compared to control. Time-dependent Cu accumulation in all tissues increased with increasing the Cu dose. The percentage of total Cu found in remaining tissues (head, bones, fins, etc.) decreased more in the CuSO 4 than Cu-NPs treatment after 25 days, but increased in all other tissues (especially in liver). Compared with the control, either Cu-NPs or CuSO 4 induced higher malonaldehyde concentration in tissues by overwhelming total superoxide dismutase activity, total glutathione concentration and Na + /K + -ATPase activity, but the opposite results were recorded for the brain. With increasing the CuSO 4 or Cu-NPs dose, apoptosis was exacerbated in the liver and gills, more so by CuSO 4 than Cu-NPs. Overall, these findings showed that Cu-NPs had the toxic effects similar to dissolved Cu; hence, Cu-NPs need to be included in the assessment of toxicological impacts in the aquatic environment.
研究领域:
环境科学
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毒理学
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水产养殖学
✓ 已验证
DOI
PubMed
高影响因子
ACS Applied Energy Materials
·
2024
·
IF 5.4
Fancheng Meng,Peng Chen,Shulin Li,Na Cao,Sheng Cheng,Xiangfeng Wei,Jiangtao Di,Jiehua Liu
Compared with the rapidly developing lithium- and sodium-ion batteries, zinc-air batteries (ZABs) offer the advantages of safety and high energy density. Nevertheless, their applications in underwater conditions are currently limited due to the low oxygen levels in such environments. Drawing inspiration from fish gills, we have successfully constructed a self-breathing zinc-air battery (ZAB) that can extract dissolved oxygen from water and convert it to electrical energy. By utilizing a Co2Fe-NC catalyst and a large-window cathode, the ZAB demonstrates a continuous discharge duration of 68.6 h in the underwater condition, thanks to its well-engineered architecture, optimized catalyst, and gas diffusion layer. Meanwhile, the underwater ZAB outperforms fish gills as it also operates efficiently in air, exhibiting a high capacity of 740 mA h g–1 and a high power density of 320 mW cm–2. Additionally, prolonged cycling tests indicate the ZAB’s capability for stable charging and discharging with minimal potential variation. This study introduces an innovative approach to the development of breathable underwater metal–air batteries.
研究领域:
材料科学
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能源技术
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仿生学
✓ 已验证
产品关联
DOI
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2021
·
IF 7.963
Hui Guo,Zhi Liang,Peihua Zheng,Ling Li,Jian'an Xian,Xiaowen Zhu
Nonylphenol (NP) is considered as one of the persistent organic pollutants (POPs) in the environment. Pacific white shrimp Litopenaeus vannamei is the predominant species in China, which is frequently affected by environmental pollutants. However, potential toxicity mechanism of NP in shrimp has not been comprehensively studied. To explore the physiological changes and molecular mechanism involved in NP exposure of shrimp, we analyzed histological alterations, apoptosis and transcriptional responses of L. vannamei subjected to NP. Results indicated that significant changes in the histoarchitecture of the gills were observed after NP exposure for 3, 12 and 48 h. Apoptosis was also detected in a time-dependent manner. Numerous differentially expressed genes (DEGs) were obtained at 3 h, 12 h and 48 h after exposure. On the basis of the expression patterns over the time course, these DEGs were classified into 12 clusters. GO and KEGG enrichment analysis of these DEGs was carried out and a dynamic and global view was obtained in shrimp after NP exposure on a transcriptome level. In addition, 15 DEGs involved in immune response, apoptosis, DNA repair, osmoregulation etc. were selected for qRT-PCR validation. The expression patterns of these DEGs kept a well consistent with the high-throughput data at different timepoints, which confirmed the accuracy and reliability of the transcriptome data. All the results demonstrated that NP exposure might lead to impairments of biological functions in gills, alter immune and antioxidant response, compromise DNA repair and anti-apoptosis abilities of shrimp, cause severe histopathological changes and eventually trigger apoptosis. The present study enriched the information on the toxicity mechanism of crustaceans in response to NP exposure.
研究领域:
环境毒理学
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分子生物学
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水产养殖
✓ 已验证
DOI
PubMed
高影响因子
AQUACULTURE
·
2025
·
IF 3.9
Lei Yang,Adili Abudu,Kecheng Zhu,Tong Han,Cunyu Duan,Yu Chen,Xiaolong Li,Gang Shi,Chunhua Zhu,Guangli Li,Changxu Tian
The spotted scat ( Scatophagus argus ), known for its robust osmoregulatory abilities as a euryhaline fish, represents a promising candidate for large-scale aquaculture in saline-alkaline waters. In this study, spotted scat were exposed to control conditions and a treatment group with alkalinity at 30 mmol/L for varying durations (0 h, 12 h, 24 h, 48 h, 72 h, and 96 h). Histopathological analysis, biochemical assays, and transcriptomic evaluation were employed to examine the impact of alkalinity stress on gill tissue. The results indicated that gill tissues exhibited varying degrees of damage when compared to the control group across different time points. Prolonged alkalinity stress resulted in an increased number of chlorine-secreting cells and caused the gill lamellae to curve, display inconsistent orientation, and swell at the base. Biochemical analyses revealed that acute alkalinity stress disrupted oxidative balance, leading to decreased activities of superoxide dismutase (SOD) and catalase (CAT), with a notable minimum observed at 96 h. In contrast, the activities of glutathione peroxidase (GSH-Px), acid phosphatase (ACP), alkaline phosphatase (ALP), and malondialdehyde (MDA) concentration gradually increased with extended alkaline exposure. Transcriptomic analysis of gill tissues from the control and 24 h acute alkalinity stress groups revealed significant changes in gene expression, identifying 513 differentially expressed genes (DEGs), with 292 up-regulated and 221 down-regulated. These DEGs were significantly enriched in pathways related to drug metabolism-cytochrome P450 and drug metabolism-other enzymes. Additionally, pathways involved in arachidonic acid metabolism, as well as arginine and proline metabolism, were found to play critical roles in adapting to alkalinity stress. This study contributes to a valuable insight into the molecular mechanisms underlying alkali resistance in spotted scat.
研究领域:
水产养殖学
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分子生物学
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环境毒理学
✓ 已验证
产品关联
DOI
高影响因子
Comparative Biochemistry and Physiology D-Genomics & Proteomics
·
2025
·
IF 2.4
Changqing He,Youheng Huang,Silvana Rahayu,Hao Liu,Yang Huang,Gang Shi,Huapu Chen,Guangli Li,Chunhua Zhu,Mouyan Jiang
The leopard coral grouper ( Plectropomus leopardus ), an increasingly important species in marine aquaculture, has garnered significant research interest due to its high market value. Despite extensive research on ovarian growth and development in fish, the molecular mechanisms governing lipid droplet formation and lipid deposition in P. leopardus remain poorly understood. In this study, we conducted transcriptomic analyses of P. leopardus ovaries at three developmental stages: primary growth (PG), pre-vitellogenesis (PV), and mid-vitellogenesis (MV). A total of 534,847,090 raw reads were obtained from nine cDNA libraries, leading to the identification of 19,155 genes with 13,817 genes expressed at all stages. Differential analysis showed that 1012, 2609, and 4039 genes were up-regulated, while 168, 277, and 577 genes were down-regulated in the three comparisons, respectively. Functional enrichment analyses highlighting the critical roles of differentially expressed genes (DEGs) in lipid transport (such as fatp1 , fatp4 , fatp6 , apoeb , lpl and fabps ), fatty acid metabolism (such as elovl6, acsl1 , dgat2 and gpat4 ) and phospholipid metabolism (such as ept1 , chka and pla2g15 ). These findings underscore their contribution to lipid droplet formation and deposition. Furthermore, key signaling pathways, including Wnt, mTOR, PPAR and PI3K/Akt, were implicated in regulating these processes. The reliability of the RNA-seq data was confirmed through qPCR validation of 10 lipid-related genes. Based on these results, we propose a model for lipid droplet formation and lipid deposition during ovarian development in P. leopardus . This study advances our understanding of ovarian development in P. leopardus and provides a foundation for future research on marine fish reproduction, with potential applications in species conservation and aquaculture management.
研究领域:
水产养殖学
·
分子生物学
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生殖生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Animals
·
2022
·Vol. 12
·期 14
·1791
·
IF 3.231
Zhenkun Xu,Hongzhi Zhang,Meijie Guo,Dan Fang,Jun Mei,Jing Xie
Simple SummaryFish are vulnerable to nitrite stress and display variations in physiologic parameter and stress response. In this study, the effects of nitrite on the stress response of large yellow croaker were evaluated. Large yellow croaker were exposed to four (i.e., 0, 29.36, 58.73, and 88.09 mg/L) nitrite concentrations for 48 h. The results of this study indicated that nitrite exposure could induce oxidative stress in the gills and serum of large yellow croaker. The result of immunoglobulin and lysozyme levels indicated that nitrite exposure could cause an immune response in large yellow croaker. In addition, nitrite stress changed the morphology of the gill of large yellow croaker.AbstractNitrite is a common pollutant in aquaculture water, and nitrite toxicity that negatively affects aquatic species is common in aquaculture systems when the water quality is low. Therefore, the present research aimed to evaluate the effect of acute nitrite exposure on the hematological parameters, antioxidant enzymes, immune response, and gill morphology of large yellow croaker (Larimichthys crocea). The fish were randomly separated and exposed to four (i.e., 0, 29.36, 58.73, and 88.09 mg/L) nitrite concentrations for 48 h. The fish blood and gills were collected at 0, 12, 24, 36, and 48 h of nitrite exposure for further analysis. In hematological parameters, the results showed that the levels of hemoglobin, triglyceride, and total cholesterol in blood significantly decreased (p< 0.05) in all nitrite-treated samples after 12 h, while the contents of methemoglobin in blood significantly increased (p< 0.05) in these treatments. After 48 h of nitrite exposure, the levels of cortisol in serum showed a 94.5%, 132.1%, and 165.6% increase in fish exposed to 29.36, 58.73, and 88.09 mg/L nitrite, respectively. The nitrite (i.e., 29.36, 58.73, and 88.09 mg/L) exposure significantly increased (p< 0.05) the levels of antioxidant enzymes (i.e., catalase and glutathione) in the gill and serum after 12 h of exposure compared with the control. The lysozyme levels in serum decreased in the nitrite (i.e., 29.36, 58.73, and 88.09 mg/L) exposure samples. It was found that immunoglobulin levels in the 29.36, 58.73, and 88.09 mg/L nitrite-treated samples (i.e., 1.86, 1.58, and 0.74 μg/mL, respectively) were lower than that of the control (2.56 μg/mL). In addition, the surface of the gill lamellae displayed deformation and contraction after 48 h of nitrite, especially in the fish exposed to 88.09 mg/L nitrite. These results indicate that the nitrite exposure induced the oxidative stress, affected the immune response, and changed the gill morphology, leading to nitrite poisoning in large yellow croaker.Keywords:acute exposure;gill morphology;immune response;nitrite stress;oxidative stress
研究领域:
水产养殖学
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环境毒理学
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鱼类生理学
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产品关联
DOI
PubMed
高影响因子
ENVIRONMENTAL TOXICOLOGY
·
2013
·Vol. 29
·期 12
·1460-1470
·
IF 2.708
Mingbao Feng,Ruijuan Qu,Ying Li,Zhongbo Wei,Zunyao Wang
Hexabromobenzene (HBB) is a novel brominated flame retardant (BFR) with ample evidence of its ubiquitous existence in the aquatic ecosystems. However, to date, the toxicological effects of this BFR on fish have been inadequately researched. The present study was conducted, based on an in vivo model, to investigate HBB-induced biochemical changes in liver and gill tissues of Carassius auratus after medium-term exposure to different concentrations (10, 150, and 300 mg/kg) for 7, 14, and 25 days. Oxidative stress was evoked evidently for the prolonged exposure, demonstrated by significant inhibition in antioxidant enzymes activities including superoxide dismutase, catalase, glutathione reductase, glutathione peroxidase, and glutathione S-transferase, and a decrease in reduced glutathione level, as well as simultaneous elevation in malondialdehyde content. Moreover, Na + , K + -ATPase activity, and protein level were remarkably reduced in fish tissues. Based on the integrated biomarker response, the toxic potency in each treatment was distinguished, and the more severe stress was mainly noted with the increasing concentrations and the extending durations. It was also observed that liver exhibited more pronounced alterations in biochemical parameters than gill, probably indicating the vulnerability of liver to HBB-triggered oxidative stress. Taken together, the results of this study clearly showed that HBB was capable of inducing oxidative stress and inhibiting Na + , K + -ATPase activity in different tissues of C. auratus after medium-term exposure. © 2013 Wiley Periodicals, Inc. Environ Toxicol 29: 1460–1470, 2014.
研究领域:
环境毒理学
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水生生态学
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生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Future Foods
·
2025
·
IF 7.2
Yan Zhu,Lingling Zhu,Zitao Guo,Wei Zhang,Wengang Jin,Ruichang Gao
Sturgeon gills, a by-product of sturgeon processing, constitute approximately 2% of the fish's total body weight but are largely underutilized, resulting in significant protein resource waste. This study aimed to isolate antioxidant peptides from sturgeon gill protein hydrolysates and evaluate their protective effects against oxidative damage in HepG2 cells. Using ultrafiltration, gel filtration, and RP-HPLC, 1,396 peptides were identified, among which FPGLP (FP5), GIPGF (GF5), and APDPF (AF5) showed the lowest binding energies to MPO (-8.6, -8.3, and -8.1 kcal/mol, respectively). These peptides significantly reduced intracellular ROS levels, and decreased cell apoptotic rates and inhibited ROS-mediated MAPK pathway phosphorylation. At 50 μM, FP5, GF5, and AF5 decreased ROS levels by 36.14%, 38.56%, and 33.75%, respectively, and suppressed phosphorylation of ERK, JNK, and p38. In conclusion, this study provides a theoretical basis for the high-value utilization of sturgeon by-products and their potential applications.
研究领域:
食品科学
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生物化学
·
分子生物学
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药理学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）