INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2026
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IF 8.7
Hong Jiang,Xingyu Guo,Luyao Wen,Xinying Zhang,Qingcong Wei
Skin is vulnerable to injuries that can lead to bacterial infection and prolonged inflammation, impeding the healing process. To address this, we developed an antibacterial and anti-inflammatory supramolecular-polymeric composite hydrogel for accelerated wound healing. The hydrogel is formed by integrating paromomycin sulfate-loaded G-quartet (G4) supramolecular nanofibers with a polymeric network of carboxymethyl chitosan grafted with phenylboronic acid (CMCS-PBA). The antibiotic, paromomycin sulfate, was in-situ loaded via dynamic Schiff base linkages with the aldehyde groups on the G4 fibers, enabling a controllable release. While the pure G4 hydrogel is mechanically weak, the introduced CMCS-PBA network significantly enhances the mechanical strength through multi-valent boronate ester bonds and supramolecular co-assembly, making it suitable for wound sites requiring mechanical robustness. The resulting composite hydrogel exhibits excellent tissue adhesion, hemostatic ability, and biocompatibility. It demonstrated potent antibacterial efficacy in vitro and in vivo. In a full-thickness mouse skin wound model, the hydrogel markedly accelerated wound closure and effectively modulated the wound microenvironment by reducing pro-inflammatory cytokines (TNF-α and IL-6) and elevating anti-inflammatory cytokine (IL-10). This work pioneers a synergistic supramolecular-polymeric strategy, yielding a multifunctional platform that surpasses the limitations of traditional single-network hydrogels, offering a versatile therapeutic strategy for managing infected and inflammatory wounds.
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FOOD CHEMISTRY
·
2026
·
IF 10.4
Xiaoyu Cao,Sisheng Li,Qianqian Xu,Zilun Lei,Bo Jiao,David Julian McClements,Hai Yan
pH-responsive hydrogel microcapsules were fabricated from pea protein isolate (PPI) and low-methoxyl pectin (LMP) for the targeted intestinal delivery of Lacticaseibacillus paracasei YWC-01, a probiotic capable of biodegrading uric acid precursors in vitro . Microgels with interpenetrating networks were fabricated via emulsion-templating: probiotics, PPI, LMP, CaCl₂, and glucono-δ-lactone were emulsified in oil and crosslinked by Ca 2+ . Optimized formulation (5% w / v PPI) achieved 94.80% encapsulation efficiency. Microgel size (43–63 μm) was controlled by adjusting Span 80 surfactant concentration. FTIR confirmed probiotic encapsulation via noncovalent interactions, while TEM revealed bacterial clustering within hydrogel voids. In vitro digestion demonstrated exceptional protection: encapsulated probiotics maintained >9 log 10 CFU/g viability (<0.4 log reduction), versus 3.2 log reduction in free cells. Metabolomics indicated encapsulation preserved bioactivity and significantly upregulated functional metabolites potentially enhancing gut microbiota modulation and anti-inflammatory effects. This work establishes an effective oral delivery platform that significantly enhances probiotic gastrointestinal survival and enables targeted release.
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ANALYTICA CHIMICA ACTA
·
2026
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IF 6.1
Yali Yang,Hui Ding,Kaijun Quan,Hongwei Zhao,Mingxia Sun,Zhimin Yang,Xiuhui Liu,Hongdeng Qiu,Jia Chen
Background To address the limitations of traditional single-mode stationary phases in separating complex samples, mixed-mode or multi-mode chromatographic stationary phases, which can provide two or more interaction mechanisms, have attracted extensive research interest. Among them, sulfonated pyrene derivatives present a promising candidate ligand, as they synergistically retain the hydrophobicity and π-π interaction capability of the pyrene moiety along with the hydrophilicity and strong cation-exchange capacity provided by the sulfonic acid groups. This synergistic multi-mechanism design significantly enhances separation selectivity for diverse and complex components. Results A pyrene trisulfonic acid-functionalized silica stationary phase (Sil-PyTSa) was successfully prepared and evaluated. In hydrophilic interaction liquid chromatography (HILIC) mode, Sil-PyTSa could efficiently separate nucleobases and nucleosides, ginsenosides, and alkaloids. For nucleobases and nucleosides, it utilized hydrophilic, electrostatic, and π-π interactions, showing superior retention and selectivity over a control HILIC column. In reversed-phase liquid chromatography (RPLC) mode, favorable separation of PAHs was achieved via hydrophobic and π-π interactions, enabling faster analysis and lower solvent consumption than a C18 column. In ion-exchange chromatography (IEC) mode, baseline separation of 14 rare earth elements (REEs) and removal of Fe/U impurities were accomplished. The column efficiency reached 95,920 N/m for naphthalene. Additionally, Sil-PyTSa achieved effective separation for REEs and iron (Fe) in a simulated NdFeB leaching solution and detected nucleobases and nucleosides in Angelica, indicting promising potential for analyzing complex matrices. Significance and novelty This work developed a novel multi-mode chromatographic stationary phase with multiple interactions mechanisms to enable high-performance separations in HILIC, RPLC, and IEC modes. It demonstrates good separation efficiency and selectivity, low solvent consumption, and high stability. This offers a versatile and green solution for the separation of complex samples with diverse analytes.
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Talanta Open
·
2026
·
IF 5.1
Mei Liu,Xuehong Min,Die Hu,Xiaonan Zhang,Siyi Zhang
In this study, we developed catechol-imprinted magnetic molecularly imprinted polymers (Ca-DFFPBA-MMIPs) via a dual-affinity strategy combining boronate affinity and a two-step template immobilization protocol. The Ca-DFFPBA-MMIPs exhibited high affinity and selectivity for catecholamines (norepinephrine, epinephrine, and dopamine) under neutral conditions. The adsorption capacity reached a plateau at pH 6.5–7.0, and the adsorption time was optimized to 40 minutes. The MMIPs showed excellent selectivity for the target catecholamines over structurally related interferents. The Ca-DFFPBA-MMIPs also demonstrated good reusability, retaining ≥90% of their initial adsorption capacity after seven consecutive cycles. The analytical performance of the Ca-DFFPBA-MMIPs was validated in terms of linear range, coefficient of determination (R²), limit of detection (LOD), limit of quantification (LOQ), and intra- and inter-day precision (RSD). The LODs were 12–31 ng mL⁻¹ and LOQs were 40–103 ng mL⁻¹, with RSDs of 0.87–1.13% (intra-day) and 1.75–1.95% (inter-day). The Ca-DFFPBA-MMIPs were successfully applied to the selective extraction of catecholamines from equine urine (enrichment factors of 18–27), with recoveries ranging from 64.5% to 105.7%. This study provides a new approach for the analysis of catecholamines in complex matrices and highlights the potential of Ca-DFFPBA-MMIPs as a practical nanosorbent for efficient and selective enrichment of catecholamines and related compounds.
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Food Bioscience
·
2026
·
IF 6.2
Xi Hu,Ruxuan Tan,Xueling Qin,Qingyun He,Zhihua Huang,Lizhong Nong,Junwei Qin,Zhenqiang Wu
Hyperuricemia is associated with dietary purine intake, dysregulated purine metabolism, and impaired uric acid excretion. This study aimed to isolate multifunctional LAB from fermented foods and apply the selected strain to enhance the functional potential of Smilax glabra Roxb. fermentation. Among 79 isolates, 46 were identified as LAB. Limosilactobacillus fermentum R-9 showed efficient degradation of inosine, guanosine, and adenosine, while exhibiting favorable gastrointestinal tolerance and safety-related probiotic properties. Its survival rates after simulated gastric and intestinal digestion were 82.28% and 67.18%, respectively. Genomics revealed genes related to nucleoside hydrolysis, purine/pyrimidine transport, nucleotide salvage, starch utilization, α-glucan metabolism, and β-glucosidase-related glycoside transformation, supporting the dual potential of R-9 for purine metabolism and adaptation to starch-rich S. glabra . L. fermentum R-9 was further used to ferment Smilax glabra Roxb., a starch-rich medicinal and edible plant material containing flavonoid glycosides. After 4 days of fermentation, total phenolics increased from 0.384 to 0.572 mg GAE/mL, and total flavonoids increased from 2.11 to 3.02 mg RE/mL. Xanthine oxidase (XOD) inhibitory activity increased from 16.58% to 70.81%, accompanied by enhanced DPPH and ABTS radical-scavenging activities. Astilbin reached 59.75 μg/mL, increasing by 33.79%. Mechanistically, L. fermentum R-9 fermentation promoted acidification, amylase and β-glucosidase activities, starch matrix disruption, and flavonoid release. These findings highlight L. fermentum R-9 as a promising starter for developing fermented Smilax glabra Roxb. products with enhanced antioxidant and xanthine oxidase inhibitory potential.
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BIOCHEMICAL SYSTEMATICS AND ECOLOGY
·
2026
·
IF 2.2
Qiucheng Luo,Gangxiang Fei,Xiaoguang Tian,Shuang Han,Jingqiang Guan,Bo Song,Longzhan Gan,Yeming Zhou,Zhijie Wang,Hongbo Lan,Xiao Zou
Two chemotypes, CTA and CTB, were identified in Cordyceps chanhua for the first time. • Intracellular nucleosides were key markers of chemotype differentiation. • CTA showed significantly higher intracellular uridine, guanosine, adenosine, and HEA than CTB. • Altitude and precipitation were the major environmental factors associated with chemotype differentiation. • Intracellular nucleosides showed significant negative correlations with altitude and precipitation and strong positive correlations with each other. Cordyceps chanhua is a valuable medicinal and edible fungus with great potential for functional food development because of its bioactive metabolites with significant pharmacological activities. However, samples from different geographical origins show significant variations in metabolite composition and content. Existing studies are mostly limited to local regions and lack systematic evaluation based on multi-regional samples, particularly regarding chemotype classification and its environmental driving mechanisms. Therefore, this study systematically analyzed the differences in bioactive metabolites, chemotype differentiation patterns, and their environmental driving factors using multi-regional samples. A total of 123 C. chanhua samples were collected from 16 regions across 8 provinces in China. Key bioactive metabolites, including uridine, guanosine, adenosine, N6-(2-hydroxyethyl)-adenosine (HEA), cordycepic acid, extracellular polysaccharides, and ergosterol, were determined in liquid fermentation broth. The results showed significant geographical differences in metabolite accumulation. Hierarchical cluster analysis (HCA) and principal component analysis (PCA) classified the samples into two chemotypes, Chemotype A (CTA) and Chemotype B (CTB). CTA exhibited significantly higher intracellular nucleoside contents than CTB ( p < 0.01), and intracellular nucleosides were identified as the core variables driving chemotype differentiation. Environmental analysis showed that CTA was mainly distributed in low-altitude and low-precipitation regions, while intracellular nucleosides were significantly negatively correlated with altitude and precipitation. Correlation network analysis further revealed synergistic accumulation patterns among multiple metabolites. This study revealed the geographical variation characteristics of bioactive metabolites in C. chanhua , identified its chemotypes for the first time, and clarified that intracellular nucleosides are key chemical markers for chemotype classification. Download: Download high-res image (340KB) Download: Download full-size image
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FOOD CHEMISTRY
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2026
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IF 10.4
Bo Wang,Feng Zhao,Zheng Zhou,Ji Wang,Lei Qin,Xuhui Huang
Studied hazardous compound changes in the thermal processing of 7 animal foods. • Hazards: acrylamide, 5-hydroxymethylfurfural, 13 heterocyclic aromatic amines. • AA, 5-HMF, and 13 HAAs in all animal foods increased with roasting temperature. • Low moisture and high carbs promoted AA; high protein favored HAAs. • Amino acids and dipeptides had negative correlations with hazardous compounds. The thermal processing of food is often accompanied by changes in composition and the generation of hazardous compounds. However, the unclear mechanisms linking food composition to related hazardous compounds hinder the advancement of inhibition technologies for hazardous compounds formed during thermal processing. In this paper, the mechanisms of 15 kinds of associated hazardous compounds produced by 7 different animal-derived food samples during the roasting process were investigated. When the roasting temperature was 260 °C, mutton exhibited the highest acrylamide content at 0.11 μg/g, chicken had the highest HAAs (heterocyclic aromatic amines) content at 0.17 μg/g, and Antarctic krill showed the highest 5-hydroxymethylfurfural content at 0.72 μg/g. notably, the compounds closely related to the production of associated hazards during thermal processing include amino acids, small dipeptides, and certain carbohydrate compounds. The results of this study have positive significance in improving food safety and optimizing production processes. Download: Download high-res image (136KB) Download: Download full-size image
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Journal of Materials Chemistry B
·
2023
·Vol. 11
·期 6
·1232-1239
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IF 7
Nan Wang,Kangkang Yu,Kun Li,Xiaoqi Yu
Supramolecular hydrogels have received widespread attention due to their soft texture, strong hygroscopicity, and good biocompatibility. These materials have become particularly attractive for sensing, tissue engineering, fluorescence encoding, wound healing, etc. Inspired by the assembly of G-quadruplexes, we choose the boric acid/polyvinyl alcohol/guanosine system to construct a novel triple-network supramolecular hydrogel “tri-BA@PVA/G” via non-covalent cross-linking, and the effect of the concentration of each component on the hydrogel stability was systematically revealed at the same time. Then, the biocompatibility, shape adaption and optical information storage capacity, the rapid hemostatic ability and the ability of the hydrogel to promote wound healing were confirmed both in vitro and in vivo. These results that predict the properties and reveal prospective applications in the field of wound hemostasis have a certain guiding significance for the subsequent preparation of borate-based triple network hydrogels which can be used as wound hemostatic materials.
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INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2022
·Vol. 23
·期 22
·13738
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IF 6.208
Guorong Cheng,Zhiqiang Liu,Zhong Zheng,Fengrui Song,Xiaoyu Zhuang,Shu Liu
Metastasis is one of the main obstacles for the treatment and prognosis of breast cancer. In this study, the effects and possible mechanisms of aloe emodin (AE) and emodin (EMD) for inhibiting breast cancer metastasis were investigated via cell metabolomics. First, a co-culture model of MCF-7 and HUVEC cells was established and compared with a traditional single culture of MCF-7 cells. The results showed that HUVEC cells could promote the development of cancer cells to a malignant phenotype. Moreover, AE and EMD could inhibit adhesion, invasion, and angiogenesis and induce anoikis of MCF-7 cells in co-culture model. Then, the potential mechanisms behind AE and EMD inhibition of MCF-7 cell metastasis were explored using a metabolomics method based on UPLC-Q-TOF/MS multivariate statistical analysis. Consequently, 27 and 13 biomarkers were identified in AE and EMD groups, respectively, including polyamine metabolism, methionine cycle, TCA cycle, glutathione metabolism, purine metabolism, and aspartate synthesis. The typical metabolites were quantitatively analyzed, and the results showed that the inhibitory effect of AE was significantly better than EMD. All results confirmed that AE and EMD could inhibit metastasis of breast cancer cells through different pathways. Our study provides an overall view of the underlying mechanisms of AE and EMD against breast cancer metastasis.Keywords:aloe emodin (AE);emodin (EMD);cell metabolomics;breast cancer;metastasis
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Advanced Science
·
2023
·Vol. 10
·期 9
·2206851
·
IF 15.1
Peidong Du,Yanzhe Shen,Baoli Zhang,Shan Li,Minzheng Gao,Ting Wang,Xiaokang Ding,Bingran Yu,Zhen-Gang Wang,Fu-Jian Xu
Photodynamic therapy (PDT) is a light triggered therapy by producing reactive oxygen species (ROS), but traditional PDT may suffer from the real-time illumination that reduces the compliance of treatment and cause phototoxicity. A supramolecular photoactive G-quartet based material is reported, which is self-assembled from guanosine (G) and 4-formylphenylboronic acid/1,8-diaminooctane, with incorporation of riboflavin as a photocatalyst to the G4 nanowire, for post-irradiation photodynamic antibacterial therapy. The G4-materials, which exhibit hydrogel-like properties, provide a scaffold for loading riboflavin, and the reductant guanosine for the riboflavin for phototriggered production of the therapeutic H 2 O 2 . The photocatalytic activity shows great tolerance against room temperature storage and heating/cooling treatments. The riboflavin-loaded G4 hydrogels, after photo-irradiation, are capable of killing gram-positive bacteria (e.g., Staphylococcus aureus ), gram-negative bacteria (e.g., Escherichia coli ), and multidrug resistant bacteria (methicillin-resistant Staphylococcus aureus ) with sterilization ratio over 99.999%. The post-irradiated hydrogels also exhibit great antibacterial activity in the infected wound of the rats, revealing the potential of this novel concept in the light therapy.
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PHYTOMEDICINE
·
2022
·
IF 6.656
Yule Wang,Huimin Wu,Zhu Han,Hongda Sheng,Yuhan Wu,Yingchao Wang,Xinran Guo,Yan Zhu,Xuecai Li,Yi Wang
Background As a leading cause of death and disability, alternative therapies for stroke are still limited by its complicated pathophysiological manifestations. Guhong injection (GHI), consisting of safflower aqueous extract and aceglutamide, has been widely applied for the clinical treatment of cerebrovascular diseases , especially ischemic stroke and post-stroke recovery, in China. Recently, a series of studies have reported the positive effect of GHI against cerebral ischemia/reperfusion injury via targeting various molecular mechanisms. However, questions remain on whether treatment with GHI contributes to better functional recovery after stroke and if so, the potential mechanisms and active substances. Purpose The aim of this work was to explore the potential therapeutic possibilities of GHI for the neurological and behavioral recovery after stroke and to investigate the underlying molecular mechanisms as well as active substances. Methods The neural and motor deficits as well as cortical lesions after GHI treatment were investigated in a mouse model of transient ischemic stroke. Based on the substance identification of GHI, network pharmacology combined with an experimental verification method was used to systematically decipher the biological processes and signaling pathways closely related to GHI intervention in response to post-stroke functional outcomes. Subsequently, ingenuity pathway analysis (IPA) analysis was performed to determine the anti-stroke active substances targeting to the hub targets involved in the significant molecular pathways regulated by GHI treatment. Results Therapeutically, administration of GHI observably ameliorated the post-stroke recovery of neural and locomotor function as well as reduced infarct volume and histopathological damage to the cerebral cortex in subacute stroke mice. According to 26 identified or tentatively characterized substances in GHI, the compound-target-pathway network was built. Bioinformatics analysis suggested that inflammatory and apoptotic pathways were tightly associated with the anti-stroke effect of GHI. Based on protein-protein interaction network analysis, the hub targets (such as NF-κB p65, TNF-α, IL-6, IL-1β, Bax, Bcl-2, and Caspase-3) involved in inflammation and apoptosis were selected. On the one hand, immunofluorescence and ELISA results showed that GHI (10 ml/kg) treatment obviously reduced NF-κB p65 nuclear translocation as well as decreased the abnormally elevated concentrations of proinflammatory cytokines (TNF-α, IL-6, and IL-1β) in damaged cortex tissues. On the other hand, GHI (10 ml/kg) treatment significantly downregulated the number of TUNEL-positive apoptotic cells in ischemic cortex and effectively restored the abnormal expression of Bax, Bcl-2, and Caspase-3. Based on the results of IPA, hydroxysafflor yellow A, baicalin , scutellarin , gallic acid , syringin , chlorogenic acid , kaempferol , kaempferol-3-O-β-rutinoside, and rutin acted synergistically on core targets, which could be considered as the active substances of GHI. Conclusion Overall, the current findings showed that the beneficial action of GHI on improving post-stroke functional recovery of subacute stroke mice partly via the modulation of cortical inflammation and apoptosis. These findings not only provide a reliable reference for the clinical application of GHI, but also shed light on a promising alternative therapeutic strategy for ischemic stroke patients .
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JOURNAL OF CHROMATOGRAPHY A
·
2022
·
IF 4.601
Kaixing Luo,Yu Luo,Yanjuan Liu,Yuefei Zhang,Wei Chen,Zhengwu Bai,Sheng Tang
s In this work, a new strategy of simultaneously grafting two different functional ligands onto the silica surface was adopted to prepare a carbonyldiimidazolium/dodecyl dual-functionalized silica stationary phase (Sil-CDI-DD) by recycling the discarded commercial C18 columns as the raw materials. A full characterization of the waste silica-C18, the calcined silica, the acidified silica and the new packing materials was done to explore the feasibility of recycling the waste chromatographic columns. Hydrophobic and hydrophilic selectivity of the obtained Sil-CDI-DD was specifically investigated, and retention mechanisms in reversed-phase and hydrophilic interaction chromatography modes were discussed. The experimental results showed that the perfect combination of carbonyldiimidazolium and long-chain alkyl groups commendably enhanced the separation selectivity and efficiency of the versatile Sil-CDI-DD, which could provide multiple interaction sites for diverse target analytes, thereby contributing to the favorable resolution of hydrophilic nucleosides/nucleobases and weak polar environmental endocrine disruptors , including alkylphenols, bisphenols , steroid hormones and phthalates .
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