AMINO ACIDS
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2025
·Vol. 57
·期 1
·1-15
·
IF 2.4
Wang Jiteng,Zhang Jingyi,Li Xinyu,Xu Hanying Y.,Yang Yeshun,Zhang Jiankun,Feng Wenping,Chen Qiang,Dong Fen,Han Tao
The main objective of this experiment was to study the metabolism of arginine in juvenile largemouth bass ( Micropterus salmoides ). A total of 300 healthy fish (average weight of 25 ± 0.5 g) were randomly assigned to ten groups. Experimental fish were orally administered or intraperitoneally injected with 0.9% sodium chloride, arginine, arginine-aspartate, citrulline, and glutamate solutions, respectively. They were euthanized at 10, 30, 60, 120, and 240 min after oral administration or intraperitoneal injection, and various tissue samples were subsequently collected for analysis. The results revealed that serum ornithine and citrulline concentrations of largemouth bass were significantly increased by oral administration of arginine or arginine-aspartate ( P < 0.05). Intraperitoneal injection of arginine or arginine-aspartate solution significantly elevated the concentrations of ornithine and citrulline in the serum, liver, kidney, and muscles ( P < 0.05). The concentrations of citrulline, ornithine, and arginine in serum and muscle increased significantly at 4 h after intraperitoneal injection of glutamate ( P < 0.05). Intraperitoneal injection of citrulline significantly increased the concentrations of ornithine and arginine in the serum and muscles ( P < 0.05). The research findings demonstrate that both free and small peptide forms of arginine were rapidly degraded to ornithine due to the high arginase activity in various tissues of largemouth bass. Additionally, the pathway of synthesizing citrulline from glutamate and then arginine from citrulline may exist in largemouth bass, but the exact location of this synthesis process may differ from that found in mammals.
研究领域:
水产养殖学
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生物化学
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DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2020
·
IF 6.306
Kai Zhou,Francesca Patrignani,Yuan-Ming Sun,Rosalba Lanciotti,Zhen-Lin Xu
Ethyl carbamate (EC), a genotoxic and carcinogenic compound in soy sauce accumulated during thermal processes, has raised public health concern for its multipoint potential carcinogenic risk to human. In this work, based on the analysis of EC accumulation during thermal processes of soy sauce, ornithine and quercetin were added before thermal processes to reduce EC accumulation. A reduction rate of 23.7–63.8% in simulated solution was founded. Kinetic studies indicated that ornithine was a byproduct of alcoholysis reaction when EC formed, while quercetin could compete with the precursor ethanol and react with carbamyl compounds, which therefore prevented EC accumulation. A maximum of 47.2% decrease of EC in soy sauce was achieved, and no remarkable changes in volatile compounds profile and color of soy sauce were found. In conclusion, the addition of quercetin and ornithine before thermal processes may be preferable for the controlling of EC content in soy sauce.
研究领域:
食品科学
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毒理学
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公共卫生
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DOI
PubMed
高影响因子
BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY
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2024
·
IF 3.2
Qian Tan,Linbo Gou,Tai-Ping Fan,Yujie Cai
Clostridium aceticum DSM1496 is an acid-resistant strain in which ornithine decarboxylase (ODC) plays a crucial role in acid resistance. In this study, we expressed ODC derived from C. aceticum DSM1496 in Escherichia coli BL21 (DE3) and thoroughly examined its enzymatic properties. The enzyme has a molecular weight of 55.27 kDa and uses pyridoxal-5′-phosphate (PLP) as a coenzyme with a K m = 0.31 mM. ODC exhibits optimal activity at pH 7.5, and it maintains high stability even at pH 4.5. The peak reaction temperature for ODC is 30°C. Besides, it can be influenced by certain metal ions such as Mn 2+ . Although l -ornithine serves as the preferred substrate for ODC, the enzyme also decarboxylates l -arginine and l -lysine simultaneously. The results indicate that ODC derived from C. aceticum DSM1496 exhibits the ability to produce putrescine, cadaverine, and agmatine through decarboxylation. These polyamines have the potential to neutralize acid in an acidic environment, facilitating the growth of microorganisms. These significant findings provide a strong basis for further investigation into the acid-resistant mechanisms contributed by ODC.
研究领域:
微生物学
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生物化学
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PubMed
高影响因子
JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC
·
2017
·
IF 2.269
Kai Huang,Tao Zhang,Bo Jiang,Wanmeng Mu,Ming Miao
l -Ornithine, a non-protein amino acid, is widely used as a food supplement and nutrition product that is usually produced by the enzymatic hydrolysis of l -arginine. However, urea, a by-product, complicates purification of l -ornithine. Here, we describe a highly efficient coupled system, which involves Rummeliibacillus pycnus arginase and Jack bean urease, for the production of l -ornithine without urea residue. Although R. pycnus arginase exhibited the highest enzyme activity at pH 9.5 with Mn 2+ , it could be activated by Ni 2+ at 70 °C and pH 6.5. Kinetic study showed that, compared with Mn 2+ -containing arginase, Ni 2+ -containing arginase had a higher substrate affinity and catalysis efficiency. Based on the properties of commercial Jack bean urease and recombinant arginase, both enzymes showed robust activity at pH 6.5 and 40–50 °C. Using a coupled system involving arginase and urease for bioconversion, l -ornithine production of 37.8 g l −1 was achieved with a 99.7% molar yield within 5 h. Thus, this coupled system represents an applicable strategy for the biosynthesis of l -ornithine.
研究领域:
生物化学
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酶工程
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生物技术
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DOI
高影响因子
Food Analytical Methods
·
2017
·Vol. 10
·期 12
·3856-3865
·
IF 2.038
Zhou Kai,Liu Yao,Li Wen-Qian,Liu Gong-Liang,Wei Na,Sun Yuan-Ming,Bai Wei-Dong,Xu Zhen-Lin
A high-performance liquid chromatography with fluorescence detection (HPLC-FLD) method for ethyl carbamate (EC) determination in Chinese soy sauce was developed and used to predict EC precursors, and extraction, derivatization, and chromatographic conditions were optimized systematically. Under optimal conditions, the limit of detection, linear range, and recoveries were 3.91 μg/L, 12.87–274.87 μg/L, and 81.5–95.4%, respectively. The method precision was less than 8.9% (RSD) and no significant difference was found between EC determinations measured by HPLC-FLD and GC/MS. Using the proposed HPLC-FLD method, the EC contents in Chinese soy sauces ranged from not detected to 64.88 μg/L ( n = 19). Except for one soy sauce brand, the EC contents in low-salt solid-state fermented (LSF) soy sauces were lower than those in high-salt liquid fermented (HLF) soy sauces and were mainly affected by alcohol content. Moreover, the free ornithine and total arginine contents were significantly correlated with EC content in the soy sauce products. The results of this work provide a foundation for further study of EC formation and inhibition in Chinese soy sauces.
研究领域:
食品科学
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分析化学
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DOI
高影响因子
Aquaculture Reports
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2024
·
IF 3.2
Kangyuan Qu,Siyuan Xiong,Caiqian Huang,Xiaoxia Jiang,Lixia Tian,Shiwei Xie
Ornithine is an arginine family amino acid and is not incorporated into protein. This study evaluated its role in growth, immune response and acute NH 3 stress tolerance of Penaeus vannamei . Six diets with graded levels (0.6, 0.9, 1.2, 1.5, 1.8, 2.1 g kg −1 ) of L-ornithine were formulated, and fed the shrimp for 8 weeks. The growth of shrimp was significantly improved with the dietary ornithine levels increased from 0.6 to 1.5 g kg −1 . Feed efficiency, protein efficiency ratio and protein productive value showed a similar trend to the growth performance of shrimp. Whole body protein and muscle lipid of shrimp were significantly increased in shrimp fed 1.8 g kg −1 ornithine compared to those fed 0.6 g kg −1 ornithine. The contents of several amino acids were increased with the dietary ornithine increased to 1.5 g kg −1 . Elevated levels of ornithine increased the Nitric Oxide (NO) content and suppressed arginase activity in the hemolymph. The relative mRNA expression of superoxide dismutase , heat shock protein 70 , dorsal and caspase 3 were increased with the dietary addition of ornithine, while the mRNA level of arginase was decreased with the increasing dietary ornithine levels. After 9 h acute NH 3 stress, mortality of shrimp reduced with the increasing dietary ornithine levels. The relative mRNA levels of superoxide dismutase , heat shock protein 70 , dorsal and catalase were increased with the dietary addition of ornithine after the acute NH 3 stress. The second-order regression analysis of weight gain and survival after acute stress revealed that the suitable ornithine content in the shrimp diet was 1.4–1.5 g kg −1 .
研究领域:
水产养殖学
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营养学
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DOI
高影响因子
ACS Applied Materials & Interfaces
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2024
·
IF 8.3
Xue Lv,Haoda Zhang,Chong Li,Xiaoming Yang
Currently, carbon dots (CDs) with room-temperature phosphorescence (RTP) show bright prospects in multiple fields, owing to their tunable wavelengths and large Stokes shifts. Howbeit, obtaining the long-lived efficient RTP of CDs still encounters a different kind of challenge. Here, we originally prepared the carbon dots (CDs@β-GPA) with β-guanidinopropionic acid and diethylenetriamine pentamethylene phosphonic acid, and CDs@β-GPA exhibited the obvious green RTP when fixed on filter paper. Importantly, the introduction of boric acid (BA) into the carbon dots resulted in stabilized triplet excitons by forming the composite of CDs@β-GPA/BA through covalent coupling, and the optical band gap was effectively reduced and nonradiative transitions were inhibited, thus leading to a significantly prolonged phosphorescence lifetime of up to 1.35 s. Meanwhile, we also acquired the phosphorescent emission of CDs@β-GPA/BA excited by visible light. This strategy may broaden the approaches to produce a long-lifetime and high-efficiency RTP material. Moreover, CDs@β-GPA/BA was successfully employed in the fields of fingerprint detection and advanced information encryption.
研究领域:
材料科学
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光学
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分析化学
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DOI
PubMed
高影响因子
FOOD CHEMISTRY
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2020
·
IF 6.306
Chengsheng Jia,Xue Xia,Huiqi Wang,Muhoza Bertrand,Gang Chen,Xiaoming Zhang
In this work, synthesis of phytosteryl ornithine ester hydrochloride was studied for the first time using an intermediate phytosteryl N,N′- bis[ tert - butoloxycarbonyl ( BOC )]-ornithine ester. This method also involved esterification of phytosterols with N,N′ -bis(BOC)-ornithine and deprotection. The maximum yield was 90% and deprotection of BOC group was more than 99% using the HCl/ethyl acetate method. As a result, thermal stability and water solubility as well as emulsifying activity and stability of phytosterols were improved through coupling with ornithine, which is favorable for their application in water-based food systems. We also observed increased bioaccessibility of phytosteryl ornithine hydrochloride (4.5%) and 65% of phytosteryl ornithine hydrochloride was hydrolyzed in vitro . These results indicated that ornithine phytosteryl ester hydrochloride can reduce dissolution capacity of cholesterol in vitro , representing improved cholesterol-reducing activity, which will further expand the applications of phytosteryl ornithine ester hydrochloride.
研究领域:
食品科学
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生物化学
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DOI
PubMed
高影响因子
Molecular BioSystems
·
2013
·Vol. 10
·期 1
·74-85
·
IF 3.35
Xiaofei Chen,Haitang Wu,Yan Cao,Xiangwen Yao,Liang Zhao,Tianqi Wang,Yu Yang,Diya Lv,Yifeng Chai,Yingying Cao,Zhenyu Zhu
Background: Candida albicans, the most common fungal pathogen related to colonization and biofilm formation on the surfaces of indwelling medical devices, shows high resistance to the most commonly used antifungal drugs. In this study, an ion-pairing chromatography-porous graphitic carbon column coupled with a time-of-flight mass spectrometry (IP-PGC-TOF/MS) system was developed for targeted and untargeted profiling of metabolites involved in biofilm and planktonic growth of C. albicans. Using untargeted profiling analysis, 16 differential metabolites were screened and identified as potential biomarkers, most of which were amino acids or related compounds. Based on untargeted profiling analysis, targeted quantitative analysis of 22 amino acids was established and carefully evaluated using stable isotope-labeled internal standards. Among them, 9 amino acids that were not screened by untargeted profiling were further characterized as new biomarkers. Finally, a total of 25 potential biomarkers were screened using the combined targeted and untargeted strategy, among which 16 were characterized for the first time. Our results confirmed that amino acid metabolism and polyamine metabolism were at a high level in biofilms, except for some new biomarkers including ornithine, arginine and proline that were directly related to ornithine. Further experiments were carried out on the ornithine decarboxylase-negative (spe1Δ) mutant, and the results showed that the consumption of ornithine for putrescine biosynthesis has a significant impact on biofilm formation and may prove to be a drug target for resolving drug resistance of C. albicans. This study provides a systematic view of changes in amino acid metabolism during C. albicans biofilm formation by a combination of targeted and untargeted profiling using an original IP-PGC-TOF/MS method. It is a feasible approach for characterizing subtle variations and screening novel biomarkers from the microbial metabolome.
研究领域:
微生物学
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医学
·
分析化学
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DOI
PubMed
高影响因子
Stem Cell Research & Therapy
·
2025
·Vol. 16
·期 1
·1-19
·
IF 7.3
Qu Shuangquan,Zhao Rui,Li Ke,Tan Yutao,Li Pei,Liu Biwu,Yang Jieru,Zhou Wenhu
Background Extracellular vesicle (EV)-based cell-free therapies have emerged as a powerful alternative to stem cell transplantation in regenerative medicine, owing to their ability to promote tissue repair while avoiding safety concerns associated with live-cell therapies. However, traditional two-dimensional (2D) cell cultures used for EV production are constrained by low exosome (Exo) yields and limited biological activity. Objective This study introduces a novel and scalable three-dimensional (3D) culture platform based on a hyaluronic acid (HA) and L-ornithine methyl ester (Orn) hydrogel to enhance the production and therapeutic efficacy of stem cell-derived exosomes. Methods The HA-Orn hydrogel was fabricated via a simple and mild crosslinking strategy, forming a biomimetic matrix that promotes spontaneous spheroid formation. Exosomes derived from 3D cultures (3D-Exo) were compared with those from 2D cultures (2D-Exo) in terms of yield, molecular composition, and biological functions. Results 3D-Exo exhibited significantly increased yield and superior functional properties, including enhanced stimulation of cell proliferation, migration, angiogenesis, and extracellular matrix remodeling. In vivo, 3D-Exo treatment accelerated wound closure and reduced inflammation in a mouse skin injury model, demonstrating robust therapeutic efficacy and safety. Mechanistic studies revealed distinct miRNA expression profiles and activation of regenerative signaling pathways in 3D-Exo. Conclusion This work presents a cost-effective, scalable, and bioinspired 3D culture system for high-yield and functionally enhanced Exo production. The HA-Orn hydrogel platform offers significant translational potential for advancing cell-free regenerative therapies, particularly in the context of wound healing.
研究领域:
再生医学
·
生物材料学
·
分子生物学
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转化医学
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组织工程
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药剂学
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DOI
PubMed
高影响因子
International Journal of Electrochemical Science
·
2025
·
IF 2.4
Xinying Ma,Zhanghua Sun,Meifeng Chen,Fenghai Li,Huiping Lv,Qi Zhu
A polyornithine (Porn) film-incorporated gold nanoparticle modified glass carbon electrode (AuNPs/Porn/GCE) was prepared by electrochemical cyclic voltammetry (CV). The electrode has a good electrocatalytic effect on the electrochemical oxidation of ractopamine (RAC). The electrochemical behavior of RAC on the AuNPs/Porn/GCE was investigated by CV or differential pulse voltammetry (DPV), and a new electrochemical method for RAC detection was established in pH 6.0 phosphate buffer solution (Na 2 HPO 4 -NaH 2 PO 4 ). There was a good linear relationship between the oxidation peak current of RAC and its concentration in the range of 4.00 × 10 −8 ∼ 1.50 × 10 −6 mol/L. The correlation coefficient (R) was 0.9970, and the detection limit was 1.50 × 10 −9 mol/L. The RAC content in beef and pork samples was detected with the modified electrode, and the recovery rate was 97.2∼102.5 %, indicating that the electrode has application potential for RAC detection in actual samples.
研究领域:
分析化学
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食品科学
·
电化学
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DOI
高影响因子
International Journal of Nanomedicine
·
2018
·
IF 4.37
Fei Tong,Suhuan Liu,Bing Yan,Xuejun Li,Shiwei Ruan,Shuyu Yang
Introduction Insulin has shown antioxidation and cytoprotective effects to decrease heart ischemia/reperfusion injury (HI/RI) in diabetes mellitus (DM), but the role of insulin/poly(ethylene glycol)-carboxymethyl chitosan (PEG-CMCS) on HI/RI in DM is not known. This research explored whether insulin/PEG-CMCS revealed a protective effect on HI/RI in DM through ornithine decarboxylase (ODC)/polyamine systems.Materials and methods Diabetes was induced via streptozotocin (STZ) in Sprague Dawley (SD) rats, which suffered from HI via blocking the left circumflex artery for 45 minutes, followed by 2 hours of reperfusion. α-Difluoromethylornithine-ethylglyoxal bis (guanylhydrazone) (DFMO-EGBG) and insulin/PEG-CMCS were administered to diabetic rats to explore their roles on severity of HI/RI.Results Insulin could be fleetly and efficiently loaded via the nanocarrier PEG-CMCS at pH =6, showing efficient loading and stable release. In addition, insulin/PEG-CMCS showed significant hypoglycemic activity in diabetic rats. On the other hand, ischemia/reperfusion obviously augmented the contents of creatine kinase (CK), lactic dehydrogenase (LDH), putrescine (Pu), myocardial infarct size, and NF-κB and spermidine/spermine N′-acetyltransferase (SSAT) expressions and decreased the levels of spermine (Sp), polyamine pools (PAs), heart rate (HR), coronary blood flow (CF), left ventricular developed pressure (LVDP), and ODC expression, compared with Sham. Administration of insulin and insulin/PEG-CMCS both reduced the contents of CK, LDH, Pu, myocardial infarct size, and NF-κB and SSAT expressions and increased the levels of Sp, PAs, HR, CF, LVDP, and ODC expression, while insulin/PEG-CMCS significantly indicated the protective results, and DFMO-EGBG showed the opposite effects.Conclusion The research showed that insulin/PEG-CMCS could play a protective effect on HR/RI in diabetic rats via its antioxidative, antiapoptotic, and anti-inflammatory roles and modulating ODC/polyamine systems.
研究领域:
药理学
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分子生物学
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心血管病学
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DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）