JOURNAL OF VETERINARY PHARMACOLOGY AND THERAPEUTICS
·
2015
·Vol. 38
·期 5
·508-512
·
IF 1.189
M. Zhao,K.-Z. Xie,H.-S. Guo,A.-H. Li,X. Xie,G.-X. Zhang,G.-J. Dai,J.-Y. Wang
A residue depletion study of ampicillin (AMP) was performed after oral dosing (60.0 mg/kg and 120.0 mg/kg body weight once a day for 5 days) to laying hens, through the use of reversed-phase high-performance liquid chromatography with fluorescence detection (RP-HPLC-FLD) to achieve detection of ampicillin residue in eggs. Limit of detection was 0.5 ng/g, and limit of quantitation was 1.2 ng/g for ampicillin. Extraction recoveries of ampicillin from samples fortified at 5.0–125.0 ng/g levels ranged from 77.5% to 84.6% in albumen, 77.9% to 87.5% in yolk, and 77.9% to 88.6% in whole egg, with coefficients of variation ≤9.3%. The maximum concentrations of ampicillin in albumen, yolk, and whole egg were detected at 1, 2, and 1 day after the last administration of ampicillin, respectively. Ampicillin was not detectable in albumen at day 9 of withdrawal time, at day 10 and 11 in yolk, and day 9 and 11 in whole egg at each of those 2 dose levels. The theoretical withdrawal time of AMP in whole egg was 6.730 and 7.296 days for 60 and 120 mg/kg oral dosage, respectively. This method also proved to be suitable as a rapid and reliable method for the determination of ampicillin in other poultry eggs.
研究领域:
兽医学
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食品科学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ENVIRONMENTAL POLLUTION
·
2020
·
IF 6.793
Zhiming Xiao,Ruiguo Wang,Decheng Suo,Shi Wang,Xiaomin Li,Shujun Dong,Tong Li,Xiaoou Su
Increasing concerns over bisphenol A (BPA) as an endocrine disrupting chemical (EDC) and its adverse effects on both humans and animals have led to the substitution by structural analogs, such as bisphenol F (BPF), in many application areas. Information regarding to the carry-over of this emerging chemical in farm animals is essential for legislation and risk assessment purposes. In this study, a large-scale number of animal experiments were designed to investigate the transfer of BPF from feed to eggs. One control and three experimental groups of laying hens (72 hens per group) were fed with basal diets and BPF-contaminated feed at concentration levels of 0.1, 0.5 and 2.5 mg kg −1 , respectively, for two weeks. The hens were then fed with BPF-free diets for a further four weeks. Eggs were collected daily, and separated into egg yolk and white for BPF analysis. The effects of different levels of BPF exposure on laying performance followed a non-monotonic dose-response curve, since low level BPF (0.1 mg kg −1 ) exposure did increase the laying rate, mean egg weight and daily feed intake, while high level BPF (2.5 mg kg −1 ) exposure showed a decreasing trend. BPF residues were detected in both egg yolks and whole eggs after two days of administration, and plateau phase was achieved within 9–18 days. There are clear linear dose-response relationships between the plateau BPF concentrations in feed and eggs. The residue of BPF was found mainly in egg yolks with conjugated form and depleted slowly (still detected 21 days after feeding the BPF-free diet of the high level group). Mean carry-over rate of 0.59% BPF from feed to eggs was obtained. Compared with the carry-over rates of PCBs and dioxins, BPF showed a relatively minor trend of bioaccumulation in eggs. To the best of our knowledge, this is the first report on the deposition, depletion, and bioaccumulation study of bisphenols in farm animals. The quantity of data can therefore be helpful in the frame of risk assessment, especially for a comprehensive estimation of consumer exposure to the residues of bisphenols.
研究领域:
动物科学
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环境科学
·
食品安全
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Foods
·
2025
·Vol. 14
·期 10
·1660
·
IF 5.1
Rong Chen,Lan Chen,Mingyue Du,Qiaozhen Guo,Ciping Zhong,Jing Zhang,Xiaoqin Yu
A high-throughput method for the determination of a variety of chemical hazards in poultry muscle and egg samples was established via ultra-performance liquid chromatography–tandem triple quadrupole mass spectrometry (UPLC–QqQ-MS). The sample preparation procedure was developed based on this quick, easy, cheap, effective, rugged, and safe (QuEChERS) method and validated for 280 chemical hazards potentially present in poultry products. The target compounds in poultry samples were extracted with a 1% formic acid–acetonitrile solution (15:85,v/v), and the metal ions in the matrix were chelated by adding ethylenediaminetetraacetic acid disodium salt (Na2EDTA). The supernatant was purified using Enhanced Matrix Removal (EMR) lipid sorbent. Chromatographic gradient separation was performed on an ACQUITY UPLC BEH C18 (2.1 mm × 100 mm, 1.7 μm) column with multiple reaction monitoring (MRM) under both negative- and positive-ion mode. Internal standard calibration or matrix-matched calibration was used for the quantitation. The results showed that good linearity was achieved for each target compound with correlation coefficients (R2) ≥ 0.99. The limits of detection (LODs) ranged from 0.05 to 10 µg/kg, and the acceptable limits of quantification (LOQs) were determined to be 0.1–20 µg/kg for all 280 compounds. Approximately 90% of the target compounds exhibited mean recoveries ranging from 60% to 120%, with relative standard deviations (RSDs) within 16.2%. This method can be used for the high-throughput rapid detection of prohibited drug residues in poultry eggs due to its easy operation and high accuracy. It was applied in real sample detection, and 43 chemicals including metronidazole were found in 211 poultry samples, with a concentration range of 0.11–638 μg/kg.
研究领域:
食品科学
·
分析化学
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
·
2019
·
IF 2.983
Wei Gao,Xiao-lan Liu,Jia-yuan Li,Hong-zhen Lian,Li Mao
A porous zwitterionic monolithic column was prepared to rapidly and efficiently separate lysozyme from egg white. The monolith was synthesized in a stainless steel HPLC column (5 cm × 4.6 mm i.d.) by in-situ thermal initiated co-polymerization of N,N- dimethyl -N- methacryloxyethyl- N -(3-sulfopropyl) ammonium betaine (MSA) and ethylene dimethacrylate (EDMA). Due to the combination of quaternary ammonium and sulfonic groups on the monolithic matrix in one-pot process, the hydrophobic carbon chain and hydrophilic radical were obtained, which provided multiple driving forces for neutral, basic and acidic analytes, thus mix-mode chromatography mechanism contributed to the retention of different charged proteins. Properties such as composition, morphology and stability of the MSA-co-EDMA monolithic column were characterized by various analytical methods and the results showed that the monolith has large through-pores, good hydrophilicity and permeability. The effects of mobile phase pH and ionic strength on proteins were investigated, drawing the conclusion that the main adsorption and elution mechanism of lysozyme on MSA-co-EDMA monolith was electrostatic interaction, while those of other proteins included hydrophobic, hydrophilic and electrostatic interactions. Therefore, efficient separation of lysozyme and other proteins could be successfully achieved by switching the pH of mobile phase. Lysozyme can be adsorbed using 20 mmol/L phosphate buffer (pH 7.0) and eluted with 20 mmol/L phosphate buffer (pH 2.0). To prove the practicality of the monolithic column, it was also applied in the separation of lysozyme in egg white, which means the work has the potential for further development in proteome analysis of real biological samples.
研究领域:
生物化学
·
蛋白质组学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2016
·
IF 4.758
Yan Zhang,Jingjing Jiang,Min Li,Pengfei Gao,Lihong Shi,Guomei Zhang,Chuan Dong,Shaomin Shuang
In this paper, we developed a simple one-pot method, employing inexpensive chicken egg white (EW) as a reducing/capping reagent, for microwave-assisted synthesis of water-soluble and well-dispersed chicken egg white-protected gold nanoclusters (EW@AuNCs) with far-red/near-infrared (FR/NIR) emission. The resultant AuNCs showed strong emission at 667 nm with a quantum yield of 7.23%. The excellent photostability, large Stokes shift (>350 nm), microsecond-scale lifetime and strong emission endowed EW@AuNCs potential as a sensing probe. The photoluminescence of EW@AuNCs could be quenched upon addition of either Hg 2+ or Cu 2+ ion. After ethylenediamine (En) was used as a masking agent, the EW@AuNCs offered highly selective for determination of Hg 2+ ion. Also, the resultant EW@AuNCs probe exhibited ultra-high sensitivity toward Hg 2+ ion with a detection limit as low as 20 pM, which is 500 times lower than the limit value (10 nM) defined by the U.S. Environmental Protection Agency in drinkable water. The proposed AuNCs probe for Hg 2+ ion has a linear range of 0.0446–5.90 nM and good repeatable response to 2.0 nM Hg 2+ with a relative standard deviation of 2.8% ( n = 5). Additionally, the applicability of the sensing system was also verified by analysis of Hg 2+ ion in tap water and pond water samples.
研究领域:
分析化学
·
环境科学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF FOOD SCIENCE
·
2025
·Vol. 90
·期 3
·e70167
·
IF 3.4
Chae-Yeon Woo,Gi-Hyeok Lee,Kyung-Jik Lim,Jun-Won Kang
The use of blue light within a range of 400–470 nm holds significant potential for sanitization purposes. However, due to an extended exposure duration needed for an antibacterial effect, the utilization of a photosensitizer (PS) to increase the efficacy of the treatment becomes essential. This study investigated prospective use of onion peel extract as a PS in combination with 405 nm blue light for the inactivation of Salmonella Typhimurium, a common foodborne pathogen on eggs. Extracts were obtained using 99% ethanol, 50% ethanol, and distilled water (DW). Their photosensitizing activities were then compared. The combination of 405 nm blue light and onion peel extract using 99% ethanol reduced bacterial populations more effectively than blue light treatment alone, while also increasing reactive oxygen species generation, cell membrane permeability, lipid peroxidation, and DNA damage levels. However, the antimicrobial effect of the 99% ethanol extract did not show a concentration dependence. Spraying DW extract on eggshell treated with 99% ethanol onion peel extract at 1 mg/mL and blue light further enhanced Salmonella reduction. Liquid chromatography was conducted for component separation. However, none of the separated fractions exhibited a significant antibacterial effect, suggesting that the active compounds responsible for antibacterial activity might work synergistically in the crude extract rather than individually. In contrast, the crude extract exhibited a significant antibacterial effect, suggesting that 99% ethanol-extracted onion peel can serve as a PS, particularly in its crude state without purification, and effectively inactivate Salmonella on eggshells. Practical Application Antimicrobial blue light (aBL) in the range of 400–470 nm is a promising nonthermal technology with several advantages, including minimal impact on food quality and safety. This study optimized the concentration of onion peel extract to improve its effectiveness as a photosensitizer in aBL treatment against Salmonella Typhimurium on eggshells. These results may serve as a reference for further optimizing aBL treatments, offering a potentially sustainable and cost-effective photosensitizer for pathogen control.
研究领域:
食品安全
·
微生物学
·
食品科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
·
2025
·
IF 8.2
Qiujiang Li,Yi Hou,Dan Sun,Ce Zhu,Ruibang Wu,Ganjun Feng,Li Zhang,Yueming Song
This study explores the use of chicken egg white (EW), a rich source of natural proteins, to address challenges in wound healing management. Herein, a novel Zn2+-infused EW/GelMA (EW/Gel) hybrid hydrogel is developed, featuring an interpenetrating network (IPN) structure, where the first network consists of photo-cross-linked GelMA and the second network consists of Zn2+-infused EW (Zn-EW) through ion–protein binding. By optimizing the design and formulation, the resulting Zn-EW/Gel hydrogel exhibited enhanced mechanical stability and self-adhesive properties. In vitro experiments demonstrated that the combined effects of functional proteins and active ions within the Zn-EW/Gel hydrogel promote fibroblast proliferation and type I collagen expression, modulate the immune microenvironment, and enhance angiogenesis. The hydrogel also demonstrated excellent biocompatibility and bioactivity in vivo, showing strong promise for restoring the physiological properties of the damaged wound tissue.
研究领域:
生物材料学
·
医学工程
·
组织工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2017
·
IF 3.154
Xueke Liu,Peng Wang,Chang Liu,Yiran Liang,Zhiqiang Zhou,Donghui Liu
Beta-cypermethrin (beta-CP), an important pyrethroid insecticide, and its main acid metabolites are frequently detected in human samples. Because beta-CP may pose some risk to human health, we studied dynamics and residues of beta-CP and its metabolites in hen egg, droppings, blood, and 15 other tissues after continuous exposure. A digestive model was then used to study beta-CP’s digestive fate. Beta-CP and its metabolites significantly accumulated in tissues with high lipid contents and were readily transferred to eggs. Beta-CP was mainly metabolized into acid metabolites that accumulated in egg and edible tissues of laying hens, suggesting that humans may be exposed to beta-CP acid metabolites through food.
研究领域:
毒理学
·
食品科学
·
动物科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MOLECULES
·
2022
·Vol. 28
·期 1
·95
·
IF 4.927
Sheng-Wei Wang,Tian-Yi Wang
Lysozyme is a natural protein with a good bacteriostatic effect, but its poor inhibition of Gram-negative bacteria limits its development potential as a natural preservative. Therefore, the modification of natural lysozyme to expand the antimicrobial spectrum become the focus of lysozyme study. Egg white lysozyme has low cost, rich content in nature, is easy to obtain, strong stability, and high enzyme activity, so it can be applied in the modification of lysozyme. Egg white lysozyme was modified by chemical methods using organic acids. Caffeic acid and p-coumaric acid in organic acids were used as modifiers, and 1-Ethyl-3- (3-dimethylaminopropyl) carbodiimide hydrochloride and N-hydroxy succinimide were used as dehydration condensation agents during modification. A certain degree of modified lysozyme was obtained through appropriate modification conditions. The antibacterial properties and structure of the obtained two organic acid-modified lysozymes were compared with natural enzymes. The results showed that compared with the native enzyme, the activity of modified lysozyme decreased, but the inhibitory effect on Gram-negative bacteria was enhanced. The minimum inhibitory concentrations of caffeic acid-modified enzyme and p-coumaric acid-modified enzyme onEscherichia coliandPseudomonas aeruginosawere 0.5 mg/mL and 0.75 mg/mL, respectively. However, the antibacterial ability of modified lysozyme to Gram-positive bacteria was lower than that of the natural enzyme. The minimum inhibitory concentration of caffeic acid-modified enzyme and p-coumaric acid-modified enzyme toStaphylococcus aureusandBacillus subtiliswas 1.25 mg/mL. The peak fitting results of the amide-I band absorption peak in the infrared spectroscopy showed that the content of the secondary structure of the two modified enzymes obtained after modification was different from that of natural enzymes. In the study, two organic acids were used to modify egg white lysozyme, which enhanced the enzyme’s inhibition of Gram-negative bacteria, and analyzed the mechanisms for the change in the enzyme’s antibacterial ability from the perspective of the structural change of the modified enzyme, providing a new idea for lysozyme modification.Keywords:hen egg white lysozyme;chemical modification;antibacterial activity;secondary structure
研究领域:
生物化学
·
微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
RAPID COMMUNICATIONS IN MASS SPECTROMETRY
·
2023
·Vol. 37
·期 13
·e9518
·
IF 2
Lihua Qu,Xiaoyu Qi,Li Zhao,Yu Zhang,Ronghua Zhuge,Zhihui Hao,Congmin Liu
Rationale The residues of fipronil and its metabolites in chicken eggs pose a threat to human health, so regular monitoring is necessary. However, the pretreatments of the existing detection methods are complex and time-consuming. A simple and streamlined pretreatment method is needed to improve the detection efficiency. Method A rapid, efficient, and facile approach employing the quick, easy, cheap, effective, rugged, and safe (QuEChERS) method with online solid-phase extraction liquid chromatography tandem Q Exactive Orbitrap high-resolution mass spectrometry (online-SPE-LC-HRMS) was established and evaluated for the determination of fipronil, fipronil-desulfinyl, fipronil-sulfone, and fipronil-sulfide in chicken eggs. An improved sample preparation technique combining QuEChERS and online-SPE was developed. Negative targeted ion fragmentation scanning and targeted-selected ion monitoring of HRMS were adopted to identify and quantify the target analytes. Results The proposed pretreatment method took a few steps in <13 min to achieve excellent recoveries and negligible interference. High selectivity was acquired with the adoption of Q/Orbitrap HRMS. The limit of quantification (LOQ) of the analytes was 2.5 μg kg −1 , meeting the detection requirements of the maximum residue level enacted by the Codex Alimentarius Commission, Japan, and the United States for the sum of fipronil and its metabolites. Extraction recoveries at three spiked concentration levels were within 84.56% to 93.84%, with relative standard deviation ≤5.87%. Conclusion The established method is efficient and easy to operate and displays satisfactory LOQs, recoveries, accuracy, and precision. This approach serves as a reference method for monitoring eggs while providing potential solutions for fipronil determination in more complicated matrices.
研究领域:
食品安全
·
分析化学
·
毒理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2015
·
IF 4.169
Runyue Zhu,Zhiyong Zhao,Jianhua Wang,Bing Bai,Aibo Wu,Liping Yan,Suquan Song
In this study, a reliable and fast method using a quick, easy, cheap, effective, rugged, and safe (QuEChERS) extraction procedure without any clean-up step was developed for simultaneous extraction of 15 mycotoxins, i.e., aflatoxin B1, aflatoxin B2, aflatoxin G1, aflatoxin G2, aflatoxin M1, aflatoxin M2, deoxynivalenol, 3-acetyldeoxynivalenol, 15-acetyldeoxynivalenol, de-epoxy-DON, zearalenone, α-zearalenol, β-zearalenol, α-zearalanol, and β-zearalanol, from eggs. High-performance liquid chromatography tandem mass spectrometry was used to separate and detect all of the analytes. Electrospray ionization at both negative and positive modes and multiple reaction-monitoring mode were applied to detect these analytes. The main factors, such as extraction time, extraction solvent, evaporation temperature, and pH of the solvent, were carefully optimized to improve the extraction efficiency. The coefficients of determination of the calibration curves ranged from 0.9884 to 0.9998. The recoveries of most of the analytes were between 71.3% and 105.4% at three concentration levels, except for AFB1 that showed recovery rates of not more than 67.5% in all concentrations. The repeatability and intra-lab reproducibility of this method were both lower than 15% and 25%, respectively. The limit of quantification ranged from 0.2 μg/kg to 5 μg/kg. The matrix effect was evaluated and reduced by the use of matrix-matched calibration curves. The validated method was applied in a pilot study to analyze mycotoxin contamination in 12 eggs, and trace amounts of deoxynivalenol, 15-acetyldeoxynivalenol, aflatoxin B1, aflatoxin G2, zearalenone and β-zearalenol were detected in these samples.
研究领域:
食品科学
·
分析化学
·
毒理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Suozhu Wu,Yiqin Zhang,Xuedong Jia,Jie Mao,Hao Wu,Shurong Wang,Hongyuan Guo
Chloramphenicol (CAP) is an antibiotic that could cause serious toxic effects on the human body. Therefore, it is crucial to measure CAP levels in food products. Here we developed an electrochemical sensor based on the molecularly imprinted polymer/polyaniline/gold nanoparticles-reduced graphene oxide-chitosan (MIP/PAN/AuNPs-RGO-CS) modified electrode to determine CAP. The PAN/AuNPs-RGO-CS and MIP film provided the function of signal amplification element and specific recognition unit, respectively. CAP was detected by square-wave voltammetry using potassium ferricyanide (K 3 Fe(CN) 6 ) as a redox signaling probe. The reduction peak current of K 3 Fe(CN) 6 linearly decreased with the logarithmic CAP concentration in two distinct ranges of 1.0–8.0 nM and 10–200 nM with a low limit of detection of 63 pM. This sensor also exhibited short removal and adsorption time, good repeatability, high selectivity, excellent stability, and acceptable recoveries for milk and egg, which offered a promising avenue for ultrasensitively measuring trace antibiotics in actual samples.
研究领域:
分析化学
·
食品安全检测
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）