Foods
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2024
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IF 4.7
Jing Yang,Jing Shi,Ying Zhou,Ye Zou,Weimin Xu,Xiudong Xia,Daoying Wang
Calcium-binding peptides have gained significant attention due to their potential applications in various fields. In this study, we aimed to prepare, characterize, and evaluate the stability of calcium-binding peptides derived from chicken blood. Chicken hemoglobin peptides (CPs) were obtained by protease hydrolysis and were applied to prepare chicken hemoglobin peptide–calcium chelate (CP-Ca). The preparation conditions were optimized, and the characteristics and stability of CP-Ca were analyzed. The optimal chelating conditions were determined by single-factor and response surface tests, and the maximum calcium ion chelating rate was 77.54%. Amino acid analysis indicated that glutamic acid and aspartic acid motifs played an important role in the chelation of the calcium ions and CP. According to the characterization analysis, CP-Ca was a different substance compared with CP; calcium ions chelated CPs via the sites of carbonyl oxygen, carboxyl oxygen, and amino nitrogen groups; and after the chelation, the structure changed from a smooth homogeneous plate to compact granular. The stability analysis showed that CP-Ca was stable at different temperatures, pH, and gastrointestinal conditions. The study indicates that chicken blood is a promising source of peptide–calcium chelates, providing a theoretical basis for application in functional foods and improving the utilization value of chicken blood.
研究领域:
食品科学
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生物化学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY
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2020
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IF 3.306
Chunling Yuan,Xiu Qin,Yuanjin Xu,Xiaoping Li,Yuye Chen,Rui Shi,Yilin Wang
Carbon quantum dots (CQDs) were prepared from chicken blood by hydrothermal method, which could catalyze the oxidation of 3, 3′, 5, 5′-tetramethylbenzidine (TMB) in the presence of H 2 O 2 to generate the blue oxidized TMB (ox-TMB) with a strong absorption peak at 652 nm. However, the introduction of biothiols could induce the reduction of ox-TMB, leading to shallower blueness and lower absorbance. Based on this fact, a convenient and sensitive colorimetric method for detection of biothiols using CQDs as peroxidase mimics was established. Several factors, such as acidity, temperature, incubating time, CQDs dosage, H 2 O 2 and TMB concentrations, which might influence the response of the analysis signal, were optimized by taking cysteine (CySH) as an example. Under the selected conditions, sensitive detection of biothiols was achieved, and the limits of detection (LOD) for CySH, glutathione (GSH), and homocysteine (HcySH) were 0.4, 0.6, and 0.9 μM respectively. This method was successfully applied for the determination of CySH in human serum samples with the recovery of 91.5 %–109.3 %, indicating the potential application of this method for biothiols sensing in real samples.
研究领域:
生物化学
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分析化学
✓ 已验证
DOI
高影响因子
CHINESE JOURNAL OF CHEMICAL ENGINEERING
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2018
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IF 1.712
Yingdi Luo,Qilei Zhang,Shanjing Yao,Dongqiang Lin
The importance of immunoglobulin Y (IgY) as a specific antibody equivalent to mammalian immunoglobulin G (IgG) is well recognized. However, production of highly purified IgY is still difficult due to the lack of specific purification methods. In this study, adsorption behaviors of IgY on four mixed-mode resins with functional ligands of 4-mercatoethyl-pyridine (MEP), 2-mercapto-1-methyli-midazole (MMI), 5-aminobenzi-midazole (ABI) and tryptophan-5-aminobenzi-midazole (W-ABI) were evaluated. The results showed that high adsorption ratio were found at pH 6.0–7.0 with little adsorption under acidic conditions. The resin with ABI ligand was then used to separate IgY from immunized chicken serum. An efficient process with IgY purity of 95% and recovery of 90% was developed after optimization of loading and elution pH and injection volume. The biological activity of the purified IgY was fully maintained. These results indicated that mixed-mode chromatography with specially-designed ligands has great potential for the separation of IgY from crude feedstock.
研究领域:
生物化学
·
免疫学
✓ 已验证
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2021
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IF 7.46
Jia Ding,Lanlan Gao,Lulu Cao,Yunlei Zhou,Hao Wang,Jingrui Duan,Huanshun Yin,Jianchao Sun,Shiyun Ai
DNA hydroxymethylation is an important epigenetic modification, which plays crucial roles in many biological processes, such as gene expression and cell differentiation. Herein, a novel photoelectrochemical immunosensor was built for detecting 5-hydroxymethylcytosine (5hmC), where the perovskite Bi 4 TaO 8 Cl sensitized by 3,4,9,10-perylenetetracarboxylicacid (PTCA) was implemented as photoactive material, anti-5hmC antibody was served as target recognition reagent, ZrO 2 was used for connecting more modifications, and ferrocene was adopted as signal amplification unit. The prepared nanomaterials of Bi 4 TaO 8 Cl, PTCA and ZrO 2 were well characterized. The possible mechanism for the improved PEC response of Bi 4 TaO 8 Cl sensitized by PTCA was investigated carefully, indicated that the matched energy band facilitated the migration of photogenerated electron. In addition, the possible mechanism for signal amplification caused by ferrocene was investigated, showing that ferrocene acted as electron donor to accelerate electron transfer. Under the optimal condition, the biosensor manifested wide detection range from 0.02 to 100 nM with the low detection limit of 5.2 pM (S/N = 3). Moreover, the applicability of the biosensor was demonstrated by investigating the effect of heavy metal Cd 2+ and perfluorooctanoic acid on 5hmC content in the leaves and roots of wheat seedlings, as well as the effect of ALV-J on 5hmC content in chicken serum.
研究领域:
生物化学
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分析化学
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材料科学
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环境科学
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分子生物学
✓ 已验证
DOI
高影响因子
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