Foods
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2024
·Vol. 13
·期 3
·410
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IF 4.7
Haining Guan,Yanli Tian,Chunmei Feng,Siqi Leng,Shifa Zhao,Dengyong Liu,Xiaoqin Diao
The protein and fat in chicken skeleton can be emulsified in a boiling state to form milky white chicken soup. White chicken soup has a delicious taste, good nutritional value, a beautiful color, and volatile flavor compounds. However, cooking time significantly impacts the quality of white chicken soup. Herein, we investigated the influence of cooking time (30, 60, 90, 120, 150, 180, and 210 min) on the migration of nutrient substances and characteristics changes in white chicken soup from chicken skeletons. The results showed that nutrients such as total lipids, water-soluble protein, total sugars, solid matter, and oligopeptides in the chicken skeletons’ tissue continuously migrated into the soup during the cooking process. The total nutrient content in the chicken soup was highest after cooking for 180 min. Simultaneously, the white chicken soup obtained after cooking for 180 min had low interfacial tension and high whiteness, viscosity, and storage stability. The high stability index was associated with increased ζ potential and decreased particle size. The contact angle analysis results also indicated that the stability of the white chicken soup was improved when the cooking time reached 180 min. This research provides basic information for the production of high-quality white chicken soup.
研究领域:
食品科学
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营养学
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DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2024
·
IF 7.7
Yanlan Ma,Xinyi Yang,Zongshuai Zhu,Tianran Huang,Jichao Huang,Ming Huang
In this study, chicken bone gelatin (CBG) was extracted as a new substitute for traditional pig bone gelatin, and bacterial cellulose (BC) was used as the compound to prepare Oregano essential oil (OEO) Pickering emulsion. To explore a protein/polysaccharide emulsion system that can effectively prolong the functional activity of OEO during storage. The results indicated that the variation in CBG and BC content significantly influenced the physicochemical properties of the emulsions. The optimal formulation of OEO Pickering emulsion, prepared with a CBG-BC ratio of 6:2 ( v /v), exhibited superior characteristics including appearance, encapsulation efficiency, and stability during preservation. After 7 d of storage at 4 °C, the rheological properties remained stable, with no significant differences observed in antioxidant and antibacterial activities. It was verified in the beef fresh-keeping experiment that the shelf life of beef samples in the 6-2-2 treatment group was 6 d longer than that in the control group and 3 d longer than that in the pure OEO group. This experiment enhanced the utilization of poultry by-products and provided a valuable reference for exploring suitable protein-polysaccharide systems embedding active substances for food preservation.
研究领域:
食品科学
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生物化学
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产品关联
DOI
PubMed
高影响因子
Molecular Catalysis
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2024
·
IF 3.9
Kang Zhang,Yuewen Shao,Yuchen Jiang,Lijun Zhang,Shu Zhang,Yi Wang,Song Hu,Jun Xiang,Xun Hu
Metal/biochar catalysts are prepared by direct impregnation of metal salts with biochar produced from pyrolysis. However, the surface structure of the catalyst carrier is affected by the pyrolysis temperature. Different pyrolysis temperatures can produce different pore structures, which can affect the dispersion of active metals. To verify this, Cu/bone biochar catalysts were prepared by directly supporting copper nitrate on the biochar from chicken bone pyrolysis at 400, 500, 650, and 800 °C, respectively. Catalytic performance of Cu/bone biochar catalysts for vanillin hydrogenation was evaluated. The results showed that the hydroxyapatite produced after pyrolysis of chicken bones was relatively stable, which made the catalyst with superior activity and reusability. The catalyst prepared by pyrolysis at a lower temperature had fewer pore structures on its surface, and the active metal Cu was easily sintered. With increasing pyrolysis temperature, organic matter gradually decreased and more pore structures were generated on the surface of the catalyst carrier, which was more conducive to the dispersion of metallic Cu species. The catalyst prepared from the chicken bone pre-pyrolyzed at 800 °C exhibited uniform dispersion of metal Cu, with the average particle size of Cu particles of 11.9 nm, achieving the yield of 91.7 % for 2‑methoxy-4-methyl phenol from hydrogenation of vanillin. In-situ IR characterizations also showed that, with the increase of pyrolysis temperature, the surface functional groups of the catalyst were more conducive to the hydrogenation of vanillin.
研究领域:
催化化学
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材料科学
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生物质能
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产品关联
DOI
高影响因子
PHYTOMEDICINE
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2025
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IF 8.3
Wenying Qi,Xu Cao,Yue Chen,Hening Chen,Ningyi Zhang,Ruijia Liu,Wei Wang,Qiyao Liu,Shihao Zheng,Size Li,Xiaoke Li,Xiaobin Zao,Yong'an Ye
Background Metabolic-associated fatty liver disease (MAFLD) is a prevalent chronic liver condition globally, characterized by suboptimal treatment outcomes. Traditional therapies often fail to address the multifaceted pathogenesis of MAFLD, which involves lipid metabolism, inflammation, and gut–liver axis dysregulation. JiGuCao Capsule formula (JCF), a patented Chinese medicine, has demonstrated clinical efficacy in liver disease treatment, indicating its potential as a new therapeutic option for MAFLD. Purpose This study aimed to investigate the therapeutic effects and underlying mechanisms of JCF in treating MAFLD, particularly focusing on its impact on liver pathology, intestinal health, and gut microbiota composition. Methods A MAFLD mouse model was developed by administering a high-fat diet and 5% fructose water for 16 weeks. At week 8, mice exhibited significant steatosis, inflammation, and insulin resistance. Fifty mice were allocated into two groups: the normal diet (ND) group with 19 mice and the high-fat feed diet (HFD) group with 31 mice. Seven mice from each group were sacrificed at week 8 for serological and histopathological assessments. The remaining mice were allocated into ND ( n = 6), HFD ( n = 6), HFD + JCFL (human equivalent dose,780 mg/kg, n = 6), HFD + JCFH (threefold the human equivalent dose, 2340 mg/kg, n = 6), HFD + Polyene Phosphatidylcholine (PPC) (human equivalent dose,177.84 mg/kg, n = 6) and ND+ JCF (human equivalent dose,780 mg/kg, n = 6) groups. Daily gavage started at week 9. At week 16, after fasting, body weight and liver condition were recorded, and mice were euthanized with pentobarbital sodium. Mouse tissues and feces were collected for histopathological, molecular biological, and multi-omics analyses. Results JCF effectively slowed MAFLD progression in mice by decreasing hepatic lipid accumulation and inflammation. Treatment with JCF significantly reduced hepatic triglycerides and inflammatory markers, including TNF-α and IL-6. JCF enhanced lipid metabolism, repaired the intestinal barrier, and lowered inflammatory cytokines in the intestines, as indicated by reduced serum LPS and restored tight junction proteins expression, such as claudin-1 and occludin. Fecal microbiota analysis indicated that JCF treatment elevated Lactobacillus levels and reduced Colidextribacter levels, correlating with enhanced metabolic profiles. The primary bioactive compounds identified in JCF responsible for these therapeutic effects were betulinic acid, cholic acid, deoxycholic acid, oleanolic acid, and pectolinarigenin. Transcriptomic analysis showed that JCF regulated key pathways involved in lipid metabolism, including the pparγ–cd36 axis and modulation of ox-LDL levels. The results indicate that JCF effectively mitigates MAFLD by influencing the gut-liver axis and lipid metabolism. Conclusion JCF alleviates MAFLD by modulating the gut–liver axis and lipid metabolism. Its effects involve improving gut barrier function, regulating microbiota, and targeting the pparγ–cd36 axis. Active compounds like betulinic acid support its therapeutic potential. JCF shows promise as a novel treatment for MAFLD, with further clinical studies needed.
研究领域:
药理学
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肝病学
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微生物组研究
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代谢研究
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产品关联
DOI
PubMed
高影响因子
RENEWABLE ENERGY
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2022
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IF 8.634
Yifan Nie,Qidong Hou,Hengli Qian,Xinyu Bai,Tianliang Xia,Ruite Lai,Guanjie Yu,Mian Laiq Ur Rehman,Meiting Ju
Sulfonated carbon material is an important class of catalysts, but controllable preparation of high-performance materials for biomass transformations is still challenging. Here, we demonstrate an effective strategy to prepare mesoporous sulfonated carbon materials from chicken bones to convert carbohydrates to 5-ethoxymethylfurfural (EMF). The carbonization of chicken bones without using additional templates gave hierarchical porous carbon materials. The subsequent sulfonation by chemical reduction approach introduced 2.33 mmol/g of -PhSO 3 H group, reserved abundant mesopores but blocked micropores. The sulfonated carbon materials afforded EMF yields of 94.7% and 68.6% from 5-hydroxymethylfurfural (HMF) and fructose in ethanol within 1.5 h and 2 h, respectively, without using high boiling point co-solvents. Furthermore, the apparent activation energies for HMF and fructose conversion were 29.1 and 49.5 kJ/mol, respectively. The catalyst could be reused four times with EMF yield decreasing from 68.6% to 62.7%.
研究领域:
材料科学
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催化
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生物质转化
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化学工程
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产品关联
DOI
高影响因子
POULTRY SCIENCE
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2022
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IF 4.014
Zhengtian Lyu,Haifang Li,Xin Li,Hui Wang,Hongchao Jiao,Xiaojuan Wang,Jingpeng Zhao,Hai Lin
ABSTRACT Fibroblast growth factor 23 ( FGF23 ), a bone-derived hormone, is involved in the reabsorption of phosphate ( P ) and the production of vitamin D hormones in the kidney. However, whether and how FGF23 regulates chicken bone metabolism remains largely unknown. In the present study, we investigated the effect of FGF23 on osteogenic differentiation and mineralization of chicken bone marrow mesenchymal stem cells ( BMSCs ). First, we found that the transcription of FGF23 was inhibited by β-glycerophosphate sodium ( GPS , 5 mM, 10 mM, 20 mM) and 10 −9 M 1, 25-dihydroxyvitamin D3 ( 1, 25(OH) 2 D 3 ), but was stimulated by 10 −7 M 1, 25(OH) 2 D 3 and parathyroid hormone ( PTH , 10 −9 M, 10 −8 M, 10 −7 M). Second, overexpression of FGF23 by the FGF23 adenovirus ( Adv-FGF23 ) suppressed the formation of mineralized nodules ( P < 0.001) and alkaline phosphatase ( ALP ) activity ( P < 0.05) in both differentiated and mineralized osteoblasts. Administration of FGF receptor 3 ( FGFR3 ) inhibitor (50 nM) was sufficient to restore the FGF23-decreased ALP activity ( P < 0.05), but not for the formation of mineralized nodules. In addition, the phosphorylation of ERK increased considerably with Adv-FGF23 overexpression ( P < 0.05). Administration of an ERK-specific inhibitor (10 μM) could down-regulate the phosphorylation of ERK ( P-ERK ) ( P < 0.05) and slightly restored the Adv-FGF23-reduction of ALP activity ( P = 0.08). In summary, our data suggest that GPS, 1, 25(OH) 2 D 3 , and PTH could regulate FGF23 mRNA expression in vitro. FGF23 is a negative regulator of bone remodeling. FGF23 not only inhibits BMSCs osteogenesis through the FGFR3-ERK signaling pathway but also suppresses the mineralization of mature osteoblasts.
研究领域:
骨生物学
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细胞生物学
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分子生物学
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内分泌学
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产品关联
DOI
PubMed
高影响因子
Frontiers in Pharmacology
·
2023
·
IF 5.6
Cao Xu,Zhang Ningyi,Chen Hening,Wang Wei,Liang Yijun,Zhang Jiaxin,Liu Ruijia,Li Shuo,Yao Yuhao,Jin Qian,Guo Ziwei,Chen Yue,Gong Yuanyuan,Li Xiaoke,Zao Xiaobin,Ye Yong’an
The JiGuCao capsule formula (JCF) has demonstrated promising curative effects in treating chronic hepatitis B (CHB) in clinical trials. Here, we aimed to investigate JCF’s function and mechanism in diseases related to the hepatitis B virus (HBV). We used mass spectrometry (MS) to identify the active metabolites of JCF and established the HBV replication mouse model by hydrodynamically injecting HBV replication plasmids into the mice’s tail vein. Liposomes were used to transfect the plasmids into the cells. The CCK-8 kit identified cell viability. We detected the levels of HBV s antigen (HBsAg) and HBV e antigen (HBeAg) by the quantitative determination kits. qRT-PCR and Western blot were used to detect the genes’ expression. The key pathways and key genes related to JCF on CHB treatment were obtained by network pharmacological analysis. Our results showed that JCF accelerated the elimination of HBsAg in mice. JCF and its medicated serum inhibited HBV replication and proliferation of HBV-replicating hepatoma cells in vitro. And the key targets of JCF in treating CHB were CASP3, CXCL8, EGFR, HSPA8, IL6, MDM2, MMP9, NR3C1, PTGS2, and VEGFA. Furthermore, these key targets were related to pathways in cancer, hepatitis B, microRNAs in cancer, PI3K-Akt signaling, and proteoglycans in cancer pathways. Finally, Cholic Acid, Deoxycholic Acid, and 3′, 4′, 7-Trihydroxyflavone were the main active metabolites of JCF that we obtained. JCF employed its active metabolites to perform an anti-HBV effect and prevent the development of HBV-related diseases.
研究领域:
药理学
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病毒学
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分子生物学
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网络药理学
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肝病学
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产品关联
DOI
PubMed
高影响因子
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