OPTIK
·
2020
·
IF 2.187
Siliang Liu,Chunyu Liu,Yu Ren,Shangqi Kuang,Chengcheng Zhou,Hongxing Cai,Zhihai Yao
The chicken and skin were studied by fluorescence spectroscopy . The three-dimensional fluorescence spectra of chicken and skin were measured, It was concluded that the optimal excitation wavelength corresponding to the fluorescence peak of 330 nm in chicken was 270 nm, and that of 475 nm and 520 nm was 370 nm. The optimal excitation wavelength corresponding to the 475 nm fluorescence peak of chicken skin was 370 nm. The fluorescence spectra of chicken skin and chicken excited by the wavelength of 370 nm were analyzed. The fluorescence peak came from myoglobin at 555 nm at the beginning of roasting, and the contribution of fluorescence peak came from oxygenated myoglobin at 540 nm and 580 nm as the baking time increased. When the baking time was more than 12 min, the fluorescence intensity of oxygenated myoglobin at 580 nm was close to zero. At this point, a considerable part of myoglobin had been converted into ferric myoglobin, while Fe 3+ in ferric myoglobin could not be absorbed by human body. At this point, the skin of chicken was no longer suitable for large amounts of consumption, while the relative performance of chicken was not obvious.
研究领域:
食品科学
·
生物化学
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产品关联
DOI
高影响因子
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
·
2025
·
IF 4.6
Jiaqi Liu,Zhaohui Lian,Jianping Wu,Xiaoyu Wu,Junshan Yang,Juna Yang,Dailin Cao,Meiyu Liu
As the world's largest egg producer, China faces challenges in egg brand development by indistinct quality characteristics. This study employed untargeted and targeted metabolomics to systematically analyze 239 differentialy abundant metabolites (VIP >1, P <0.05) in breed Rongde Xiaoheiji albumen (RDA) and indigenous Taihangji albumen (THA). L-valine was identified as the most significant differential marker between breeds. Key differential metabolic pathways involved biosynthesis of amino acids and 2-oxocarboxylic acid metabolism. Targeted quantification revealed significantly lower levels of valine, leucine, and phenylalanine in RDA compared to THA, strongly correlated with isocitrate downregulation in amino acid biosynthesis. Metabolic profiling differences indicate that RDA exhibits potential superior advantages in antitumor functions, cognitive support, intestinal protection, and anti-inflammatory properties, while THA shows potential benefits in muscle protein synthesis, post-exercise recovery, neurotransmitter balance maintenance, and energy metabolism efficiency. This research provides novel perspectives for establishing metabolomic feature-based egg quality evaluation systems.
研究领域:
生物化学
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动物科学
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食品科学
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DOI
高影响因子
VETERINARY PARASITOLOGY
·
2020
·
IF 2.157
Yikai Fu,Junwen Zhou,Lifang Zhang,Chenzhong Fei,Xiaoyang Wang,Mi Wang,Chunmei Wang,Keyu Zhang,Feiqun Xue,Yingchun Liu
The treatment effect of ethanamizuril (EZL) to broiler chickens experimentally infected with 8 × 10 4 Eimeria tenella was evaluated. On the third day after infection, the broiler chickens were treated with EZL by gavage at doses of 2, 4, and 8 mg/kg body weight (bw) for once. For double administration, the challenged broiler chickens were administered EZL at doses of 1, 2, 4, and 8 mg/kg bw by gavage continually on the third day and fourth day and once a day. Throughout the experimental period, performance parameters including body weight gain, mortality, cecal lesion score, bloody diarrhoea and oocyst output were recorded. The anticoccidial efficacy was evaluated using the anticoccidial index (ACI). Meanwhile, the concentrations of EZL in chicken cecal contents were measured, and the data were analyzed with a non-compartmental model. The results indicated that EZL showed good anticoccidial activity at single dose of 4 mg/kgbw, with the corresponding ACI of 175.73. When the challenged chickens were treated with EZL under double administration, the EZL showed a medium level of anticoccidial activity at a dose of 2 mg/kg bw, with the corresponding ACI of 162.48. The maximum concentrations (C max ) of EZL in content were 2.43 ± 1.16, 4.28 ± 1.56, and 8.57 ± 1.33 mg/kg after the chickens were administrated at doses of 2, 4, and 8 mg/kg bw, respectively. The respective areas under the curve were 36.93 ± 8.91, 96 ± 16.31, and 262.76 ± 51.52 mg/kg h. The respective half-lives (T 1/2 ) were 10.82 ± 2.02, 10.53 ± 2.23, and 10.60 ± 1.50 h. The results show that when the concentrations of EZL in chicken cecal contents reached 4.28 ± 1.56 mg/kg, there is a significant therapeutic effect on chicken coccidiosis.
研究领域:
兽医学
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药理学
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寄生虫学
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产品关联
DOI
PubMed
高影响因子
Foods
·
2024
·Vol. 13
·期 3
·410
·
IF 4.7
Haining Guan,Yanli Tian,Chunmei Feng,Siqi Leng,Shifa Zhao,Dengyong Liu,Xiaoqin Diao
The protein and fat in chicken skeleton can be emulsified in a boiling state to form milky white chicken soup. White chicken soup has a delicious taste, good nutritional value, a beautiful color, and volatile flavor compounds. However, cooking time significantly impacts the quality of white chicken soup. Herein, we investigated the influence of cooking time (30, 60, 90, 120, 150, 180, and 210 min) on the migration of nutrient substances and characteristics changes in white chicken soup from chicken skeletons. The results showed that nutrients such as total lipids, water-soluble protein, total sugars, solid matter, and oligopeptides in the chicken skeletons’ tissue continuously migrated into the soup during the cooking process. The total nutrient content in the chicken soup was highest after cooking for 180 min. Simultaneously, the white chicken soup obtained after cooking for 180 min had low interfacial tension and high whiteness, viscosity, and storage stability. The high stability index was associated with increased ζ potential and decreased particle size. The contact angle analysis results also indicated that the stability of the white chicken soup was improved when the cooking time reached 180 min. This research provides basic information for the production of high-quality white chicken soup.
研究领域:
食品科学
·
营养学
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
·
2024
·
IF 4
Can Cui,Xiaojun Jiao,Xianbin Xu,Jiongzhou Gong,Jiaxin Zhou,Ge Tian,Liwei Lu,Li Li,Xianhui Huang
The presence of residues of veterinary drugs in eggs is an ongoing and significant issue of concern. Fluralaner is widely used in the prevention of ectoparasites, including the poultry red mite, but no analytical method has been reported for detection of its residues in eggs. An HPLC-MS/MS method was established for the determination of fluralaner residues in whole eggs and validated for LOD, LOQ, specificity, linearity, accuracy, precision, and matrix effects. LOD was 5 μg•kg −1 and LOQ was 65 μg•kg −1 . Recovery ranged from 97.7% to 103.7% (spiked at 65, 650, 1300, and 2600 μg•kg −1 ), while intra-assay and inter-assay coefficients of variation were consistently below 1.5%. A drug withdrawal trial was conducted with 16 laying hens administered a therapeutic dose of fluralaner in drinking water on days 0 and 7, with eggs being analyzed for 23 days. The highest concentration, 1235 μg•kg −1 , was detected on day 13. This was below the European Union maximum residue limit for fluralaner in edible chicken tissues and eggs (1300 μg•kg −1 ). Residues were detectable up to two weeks after cessation of treatment. This method is suitable for routine compliance monitoring of fluralaner residues in egg matrices and may be adapted for analysis of other edible tissues.
研究领域:
兽医学
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食品科学
✓ 已验证
产品关联
DOI
高影响因子
POULTRY SCIENCE
·
2024
·
IF 3.8
Xing-Ping Li,Jun Liang,Ze-En Li,Ming-Hui Duan,Yan Dai,Yan-Ni Zhang,Yue Liu,Yang-Guang Jin,Fan Yang
ABSTRACT This study aimed to compare the residue depletion of gamithromycin in yellow-feather and white-feather broilers, using Sanhuang and Arbor Acres chickens as typical examples, respectively. Each breed (54 chickens) received a single subcutaneous dose of gamithromycin at 7.5 mg/kg bodyweight ( BW ). Tissues, including muscle, skin + fat, liver, kidney, and injection site, were collected at 6 h, 3, 5, 7, 10, 14, 21, 28, and 35 d postdrug administration. Gamithromycin concentrations in these tissues were determined using ultra‐performance liquid chromatography–tandem mass spectrometry ( UPLC‐MS/MS ). The kinetics of gamithromycin were analyzed in different tissues using a noncompartmental method in the Phoenix software. Differences were observed in gamithromycin concentrations and kinetic characteristics in both breeds of chickens, with higher residue concentrations and longer residue times found in yellow-feathered broilers. In Sanhuang broilers, the elimination rates of gamithromycin followed this order: injection site > muscle > liver > kidney > skin + fat. The corresponding elimination half-lives ( t 1/2λz s ) in these samples were 1.22, 1.30, 1.71, 2.04, and 2.52 d, respectively. In contrast, in Arbor Acres broilers, a different order was noted: muscle > injection site > kidney > liver > skin + fat, with corresponding t 1/2λz s of 1, 1.23, 1.88, 1.93, and 2.21 d, respectively. These differences may be related to variations in pigments in various tissues of chickens of the 2 breeds. However, further investigations are warranted to discern the underlying reasons.
研究领域:
兽医学
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药代动力学
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食品科学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF AOAC INTERNATIONAL
·
2021
·Vol. 104
·期 6
·1549-1558
·
IF 1.913
Yang Yi,Yin Shuo,Wu Ling,Li Yongxin,Sun Chengjun
BackgroundThe wide usage of tetracyclines in livestock farming may result in drug residues in foods. Therefore, it is necessary to develop reliable methods for the determination of tetracyclines in foods.ObjectiveA dispersive liquid-liquid microextraction (DLLME) combined with HPLC method was developed for the analysis of six tetracyclines in eggs and chicken.MethodsAfter deproteinization, tetracyclines in acidic solutions were concentrated by vortex-assisted DLLME. After the addition of NaCl (35% for eggs and 20% for chicken), a mixture of ionic liquid 1-butyl-3-methylimidazolium hexafluorophosphate and ethyl acetate (300 μL–50 μL for eggs and 200 μL–60 μL for chicken) was used as the extractant. After centrifugation, the extract was collected for HPLC analysis.ResultsThe developed method showed good linear relationships (10.0–500 μg/kg), low method detection limits (0.219–1.42 μg/kg) and quantification limits (0.731–4.72 μg/kg), and satisfactory relative recoveries (87.1–104%), with intra-day and inter-day RSDs in the ranges of 0.853–8.62% and 1.65–11.8%, respectively. The established method was successfully applied for the determination of six tetracyclines in eggs and different parts of chicken. The contents of tetracyclines in all samples were lower than their maximum residue limits.ConclusionA DLLME-HPLC method has been developed for the analysis of six tetracyclines in animal-derived foods using ionic liquid and ethyl acetate as the extractant.HighlightsThe developed method is simple, sensitive, cost-effective, and has strong anti-interference abilities. This method has been successfully applied to the analysis of six tetracyclines in eggs and chicken.
研究领域:
食品科学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Frontiers in Veterinary Science
·
2025
·
IF 2.9
Baili Lu,Jiayu He,Shirou Pan,Junhao Wei,Bingxin Li,Nan Cao,Yunmao Huang,Yunbo Tian,Ngai Cheong,Ying Chen,Danning Xu,Wanyan Li
Atractylodes macrocephala Koidz. (AMK) and its purified polysaccharide fraction (PAMK) are known for their antioxidant, anti-inflammatory, and hepatoprotective properties, showing potential benefits for poultry liver health. This study simulated hepatic oxidative stress in late-laying hens, a physiological stage characterized by increased metabolic demands and reactive oxygen species (ROS) generation, without exogenous hepatotoxic agents. Hens were randomly assigned to three groups: control (basal diet), AMK (basal diet + 15 g/kg AMK), and PAMK (basal diet + 400 mg/kg PAMK). Both AMK and PAMK significantly reduced serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities compared to control. Notably, PAMK showed superior efficacy, decreasing malondialdehyde (MDA) levels by 35.29% vs. 32.87% in AMK, and more effectively increasing antioxidant enzymes superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px). Histopathological analysis revealed better-preserved liver structure and less inflammatory infiltration in PAMK-fed hens. Mechanistically, both treatments upregulated Nrf2 and downstream antioxidant genes, with stronger activation observed in the PAMK group. In vitro, PAMK reduced H2O2-induced ROS accumulation and apoptosis in primary embryonic chicken hepatocytes, effects that were attenuated by the Nrf2 inhibitor ML385. In conclusion, PAMK exerts superior hepatoprotective effects compared to crude AMK by modulating the Nrf2 pathway, mitigating oxidative stress, apoptosis, and autophagy. Future research should evaluate PAMK's long-term safety, synergistic potential with other natural antioxidants, and cost-effectiveness in poultry production.
研究领域:
兽医学
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动物营养学
·
分子生物学
·
氧化应激
·
天然药物药理学
·
家禽生理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Food Additives and Contaminants Part A-Chemistry Analysis Control Exposure & Risk Assessment
·
2018
·
IF 2.129
Zhaopeng Wang,Xuezhi Yu,Licai Ma,Hebing Liu,Shuangyang Ding,Zhanhui Wang,Xiya Zhang,Jianzhong Shen,Kai Wen
In this work, high affinity polyclonal antibodies for ribavirin (RBV) from new haptens were prepared and were used to analyse RBV residues in chicken muscle, eggs and duck muscle. The new haptens were synthesised with different spacers, and the best antibody was obtained with an IC50 value as low as 0.61 ng/mL in indirect competitive enzyme-linked immunosorbent assay (ELISA). The cross-reactivities with another five antiviral drugs including amantadine, rimantadine, moroxydine, zanamivir and oseltamivir were less than 0.1%, which indicated the good specificity of the antibody. An ELISA was developed based on the antibody and applied to detect RBV in multi-food matrices. The sample preparation prior to detection only needed simple dilution after trichloroacetic acid extraction. The limits of detection were 1.07, 1.18 and 1.03 μg/kg in chicken muscle, eggs and duck muscle, respectively. Recoveries ranged from 89.0% to 112.7% with coefficients of variation below 13.0%. Ten blind samples of chicken muscle were analysed simultaneously by ELISA and liquid chromatography-tandem mass spectrometry, and a good correlation between the methods was observed. The results indicated that the high affinity antibody could be applied for the simple and fast detection of RBV in multi-food matrices.
研究领域:
免疫学
·
食品科学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CONTROL
·
2025
·
IF 6.3
Yingshuang Lu,Yu Jiang,Mengyu Sun,Lu Dong,Xiaolong Xing,Yan Zhang,Shuo Wang
This study explores the formation of advanced glycation end products (AGEs) in chicken breast during roasting, focusing on the analytical characterization and simulation of dynamic models for the content of carboxymethyllysine (CML) and carboxyethyllysine (CEL), two key AGEs. To systematically investigate the formation patterns of AGEs, the chicken breasts were subjected to five roasting temperatures ranging from 180 °C to 230 °C. At each temperature, samples were roasted to achieve five distinct core temperatures, from 60 °C to 100 °C. Dynamic models for CML and CEL content were constructed using multiple linear regression and partial least squares methods, with the best-fit models identified based on their R 2 and RMSE values, with R 2 values of 0.886 for CEL, 0.954 for CML, and RMSE values indicating minimal prediction error. Our research provides a robust framework for predicting AGEs formation during roasting with validation, offering valuable insights for optimizing cooking processes to balance product quality and safety.
研究领域:
食品科学
·
生物化学
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产品关联
DOI
高影响因子
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2014
·Vol. 22
·期 6
·4654-4659
·
IF 2.757
Yao Lixian,Huang Lianxi,He Zhaohuan,Zhou Changming,Li Guoliang,Deng Xiancai
Roxarsone (ROX), a world widely used feed organoarsenic additive in animal production, can be excreted as itself and its metabolites in animal manure. Animal manure is commonly land applied with phosphorous (P) fertilizer to enhance the P phytoavailability in agriculture. We investigated the accumulation of As species in garland chrysanthemum ( C. coronarium ) plants fertilized with 1 % ( w / w , manure/soil) chicken manure bearing ROX and its metabolites, plus 0, 0.05, 0.1, 0.2, 0.4, and 0.8 g P 2 O 5 /kg, respectively. The results show that As(III) was the sole As compound in garland chrysanthemum shoots, and As(III) and As(V) were detectable in roots. Elevated phosphate level supplied more As(V) for garland chrysanthemum roots through competitive desorption in rhizosphere, leading to significantly enhanced accumulation of As species in plants. As(III) was the predominant As form in plants (85.0∼90.6 %). Phosphate could not change the allocation of As species in plants. Hence, the traditional practice that animal manure is applied with P fertilizer may inadvertently increase the potential risk of As contamination in crop via the way ROX → animal → animal manure → soil → crop.
研究领域:
植物学
·
环境科学
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产品关联
DOI
PubMed
高影响因子
Innovative Food Science & Emerging Technologies
·
2022
·
IF 7.104
Lin Lin,Canchao Mei,Xiaochen Chen,Changzhu Li,Zichun Hua,Haiying Cui
The infection of Campylobacter jejuni ( C. jejuni ) in food can cause huge economic and health costs, and liposomes based on biological switch is a promising antibacterial strategy. In this study, a bio-responsive composite liposome was designed with silk fibroin and L-fucose (FLPs) based on the chemotaxis and protease of C. jejuni , and Litsea cubeba essential oil as antibacterial agent. The oil encapsulation and protein mosaic rates of the composite liposome were 70.6 ± 0.5% and 47.9 ± 1.0%, respectively. The in vitro release experiment verified that the release rates of essential oil from the liposome exceeded 95% at 5 days. Furthermore, FLPs possessed excellent antibacterial activity , the inhibition zone was 23.5 mm and 20% ( v /v) of the composite liposomes could inactivate 99% C. jejuni . Moreover, laser scanning confocal microscopy and scanning electron microscopy images confirmed the antimicrobial activity of FLPs was better than that of other liposomes, and it could attach to bacteria well. Sprayed liposomes revealed sustained ability of retarding bacterial reproduction within 5 days with the refrigerated chicken meat inoculated with C. jejuni , and had no impact on the color and texture of the food. Hence, the bio-responsive composite liposome has a promising application in inhibiting C. jejuni growth in food. Industrial relevance In this study, the composite liposome with high encapsulation efficiency shows significant and sustained antibacterial activity, and has no negative effects on the color and texture of chicken meat. The findings of this study will facilitate the development and application of liposomes as active antimicrobial packaging in food industry.
研究领域:
食品科学
·
微生物学
·
生物技术
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）