FOOD CHEMISTRY
·
2022
·
IF 9.231
Shengyang Zhou,Lingling Guo,Xinxin Xu,Shanshan Song,Liqiang Liu,Hua Kuang,Yingyue Zhu,Liguang Xu,Chuanlai Xu
Amanitin (AMA) is responsible for human fatalities after ingestion of poisonous mushrooms, thus, a rapid and accurate detection method is urgently needed. Here, gold nanoparticle-based immunosensor with monoclonal antibody against AMA was constructed for rapid detection of alpha- and beta-amanitin (α- and β-AMA) in mushroom, serum and urine samples. Under optimized conditions, the visual limits of detection (vLOD) and calculated LOD for α-AMA and β-AMA in mushroom were 10 ng/g, 20 ng/g, and 0.1 ng/g, 0.2 ng/g, respectively. Analysis of wild mushroom samples was also performed using a strip scan reader in the 10 min range. Furthermore, in mushrooms containing amatoxins results were confirmed and compared with those determined by liquid chromatography tandem mass spectrometry. Thus, this immunosensor provided a useful monitoring tool for rapid detection and screening of mushroom samples and in serum and urine from subjects who accidentally consumed AMA-containing mushrooms.
研究领域:
毒理学
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分析化学
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医学检测
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2016
·
IF 3.926
Lei Tan,Rong He,Yongxian Li,Yong Liang,He Li,Youwen Tang
α-Amanitin and β–amanitin are the main toxins of mushroom poisoning. The application of traditional non-selective adsorbents is not satisfactory in clinical treatment of amanita mushroom poisoning due to lack of specificity adsorption capability of these adsorbents toward amanitin toxins. In the current work, we introduce a novel molecularly imprinted biomimetic adsorbent based on a ligand specificity determinant through surface imprinted strategy. Owing to the expensive price of the amanitin sources, we selected a typical common moiety of α, β–amanitin as specificity determinant to synthesize a template necessary for the preparation of molecularly imprinted polymers (MIPs). Computer simulation was used to initially select acidic methacrylic acid (MAA) and basic 4-vinyl pyridine (4-VP) together as functional monomers. The experiments further demonstrated that the synergistic interaction of MAA and 4-VP played a primary role in the recognition of α, β-amanitin by MIPs. By means of batch and packed-bed column experiment and the hemocompatibility evaluation, the resultant biomimetic adsorbent has been proved to be capable of selectively removing α, β–amanitin and possess good hemocompatibility. This novel adsorbent has great potential to find application in human plasma purification.
研究领域:
医学
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毒理学
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材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Aquaculture Reports
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2021
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IF 3.216
Shiwei Xie,Dan Wei,Lixia Tian,Yongjian Liu
Bile acid metabolism in aquatic animals usually is suppressed when fish meal (FM) is replaced by plant proteins. An eight weeks feeding experiment was conducted to evaluate the effects of dietary chenodeoxycholic acid (CDCA) supplementation on growth, immune response and intestinal health of Penaeus monodon fed a low FM diet. Three diets were formulated: high FM (25 %) diet (HF), low FM (15 %) diet (LF), and low FM diet supplemented with 0.1 % CDCA (LFC). Shrimp fed Diet LF showed lower weight gain and feed efficiency than those fed Diet HF. Low dietary FM up-regulated the mRNA level of xbp1 , and down-regulated the mRNA levels of sod , iap and NF-κB signaling pathway-related genes ( traf6 , tube ). Dietary supplementation of CDCA up-regulated the expression of relish and anti-oxidative-related genes ( sod and cat ) in the hepatopancreas and midgut. Low dietary FM impaired the intestinal fold and induced the endoplasmic reticulum swollen of intestinal epithelial cell, dietary supplementation of CDCA alleviated this problem. The intestinal microbiota diversity was lower in shrimp fed Diet LF than those fed HF. Dietary supplementation of CDCA in a low FM diet increased the intestinal microbiota diversity by decreasing the relative abundance of dominant phylum Proteobacteria. Low dietary FM increased the relative abundance of a dominant pathogenic bacterium ( Vibrio ), which was decreased by the supplementation of CDCA. These results indicated that dietary supplementation of CDCA in a low FM diet improved the growth performance, immune response and intestinal health of shrimp.
研究领域:
水产养殖学
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营养学
·
分子生物学
✓ 已验证
DOI
高影响因子
MOLECULES
·
2024
·Vol. 30
·期 1
·26
·
IF 4.2
Tingyu Ma,Jieni Wang,Xiaobo Han,Chuanbing Zhang,Yahui Xu,Leichang Cao,Shuguang Zhao,Jinglai Zhang,Shicheng Zhang
Discarded sports waste faces bottlenecks in application due to inadequate disposal measures, and there is often a neglect of enhancing resource utilization efficiency and minimizing environmental impact. In this study, nanoporous biochar was prepared through co-hydrothermal carbonization (co-HTC) and pyrolytic activation by using mixed goose feathers and heavy-metals-contaminated pine sawdust. Comprehensive characterization demonstrated that the prepared M-3-25 (Biochar derived from mixed feedstocks (25 mg/g Cu in pine sawdust) at 700 °C with activator ratios of 3) possesses a high specific surface area 2501.08 m2g−1and abundant heteroatomic (N, O, and Cu), exhibiting an outstanding physicochemical structure and ultrahigh electrochemical performance. Compared to nanocarbon from a single feedstock, M-3-25 showed an ultrahigh capacitance of 587.14 F g−1at 1 A g−1, high energy density of 42.16 Wh kg−1, and only 8.61% capacitance loss after enduring 10,000 cycles at a current density of 10 A g−1, positioning M-3-25 at the forefront of previously known biomass-derived nanoporous carbon supercapacitors. This research not only introduces a promising countermeasure for the disposal of sports waste but also provides superior biochar electrode materials with robust supercapacitor characteristics.
研究领域:
环境科学
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材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ENVIRONMENTAL RESEARCH
·
2025
·
IF 7.7
Huiting Yang,Yujia Yao,Wei Chen,Xiaohong Gu,Huihui Chen,Qingfei Zeng,Zhigang Mao,Tao Xiang
Cyanobacterial blooms can generate various toxic metabolites in freshwater, and pose serious threats to drinking water safety and human health. Although microcystins (MCs) have been detected in many freshwater ecosystems in China, little is known about the other cyanotoxins. An investigation of six eutrophic lakes (i.e. Hulun Lake, Wuliangsuhai Lake, Chaohu Lake, Taihu Lake, Xingyun Lake, and Dianchi Lake) in different geographical locations of China was performed during the summer of 2022 to determine the occurrence of various cyanotoxins (i.e. anatoxin-a (ATX), cylindrospermopsin (CYN), and MCs) in water column and their possible risks, and to evaluate the related environmental factors. MCs levels in sediment of these lakes were also investigated. MCs were the primary cyanotoxins in the water column of investigated lakes. The mean MCs contents in water column of Hulun Lake, Wuliangsuhai Lake, Chaohu Lake, Taihu Lake, Xingyun Lake, and Dianchi Lake were 3.61, 0.13, 3.60, 2.18, 0.57, and 2.56 μg/L, respectively. The total MCs levels in water column exceeded 1 μg/L in some areas of these lakes except Wuliangsuhai Lake. Replete nitrogen and/or phosphorus levels seemed to be related to MCs production. ATX can be detected in these lakes except Xingyun Lake at ng/L levels. CYN can be detected in all lakes at ng/L levels. However, the levels of ATX and CYN appear to be not significantly associated with environmental factors. MCs and CYN can pose a high or moderate risk for humans and aquatic organisms in some areas of these lakes, while ATX can pose a low or no risk for humans and aquatic organisms in most areas of these lakes. MCs can also be detected in sediment of all lakes at ng/g levels. This research emphasizes the necessity for long-term monitoring of different cyanotoxins in eutrophic lakes, and the implementation of nutrient control and management strategies.
研究领域:
生态学
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环境科学
·
毒理学
✓ 已验证
DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2019
·
IF 5.256
Xiuzhen Qiu,Weimao Chen,Yiling Luo,Yulin Wang,Yanlian Wang,Huishi Guo
α-amanitin is the most toxic amanita in mushrooms with lethal dose to humans around 0.1 mg. Kg −1 . Hence, early identification of the poison would improve survival rates and prevent lethal poisoning cases. In this study, molecularly imprinted photonic crystal (MIPC) sensor was prepared by combining molecular imprinting with photonic crystal templates and tested towards the detection of α-amanitin. In this process, synthesized moiety of α–amanitin was utilized as template, dispersed SiO 2 colloidal photonic crystal as carrier, methacrylic acid (MAA) as functional monomer , and ethylene glycol dimethacrylate (EDGMA) as crosslinker. The adsorption behavior of MIPC towards α-amanitin in ethanol solution showed shifts in diffraction peaks of MIPC upon binding with α-amanitin molecules. The reflection peak wavelength varied linearly with α-amanitin concentration according to the correlation formula: λ = 15.417 c +489.17 (R 2 = 0.9985). The recognition process was accompanied by gradual color change in MIPC film. The prepared MIPC sensor possessed wide linear range (10 −9 –10 −3 mg L −1 ), change in visual color, low detection limit (10 −10 mg L −1 ), short response time (2 min), and good reusability. The MIPC film was then tested towards the detection of α-amanitin in real biological samples (mushroom, urine, and serum) and showed reasonable shift in diffraction peaks and color change upon soaking in solutions spiked with α-amanitin at 10 −6 mg L −1 and 10 −3 mg L −1 , suggesting the suitability of the film for the rapid identification of α-amanitin in complex sample matrices. Overall, the proposed sensor looks promising for the rapid identification of α-amanitin in clinical analysis and food poisoning.
研究领域:
生物化学
·
毒理学
·
传感器技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF MOLECULAR LIQUIDS
·
2014
·
IF 2.083
Yang Yang,Yonghong Hu,Qi Zhang,Limin Cheng,Cuicui Cao,Wenge Yang,Fei Shen
Data on corresponding solid–liquid equilibrium of hyodeoxycholic acid (HDCA) in different solvents is essential for a preliminary study of industrial applications. In this paper, the solid–liquid equilibrium of HDCA in methanol, ethanol, isopropyl alcohol, butan-1-ol, ethyl acetate , acetone, acetonitrile , and acetic acid was explored at temperatures ranging from 278.15 to 333.15 K under atmosphere pressure. For the temperature range investigated, the solubility of HDCA in the solvents increased with increasing temperature. The modified Apelblat model, the Buchowski–Ksiazaczak λh model, and ideal model were adopted to describe and predict the change tendency of solubility. Besides, the calculated thermodynamic parameters indicated that in each studied solvent the dissolution process of HDCA is endothermic. Based on the analysis above, ethanol is considered to be the best solvent in crystallization process of HDCA.
研究领域:
化学工程
·
药物化学
✓ 已验证
产品关联
DOI
高影响因子
Frontiers in Plant Science
·
2022
·
IF 6.627
Liu Silin,Wang Wenhui,Chen Jiaoyun,Ma Zhiyu,Xiao Youping,Chen Zhongwen,Zhang Ying,Du Xiao,Mu Yinghui
Astragalus sinicus L. (milk vetch), a versatile plant that has a soil-enriching effect as green manure, is widely planted in the temperate zone of China. In previous experiments, milk vetch incorporated into the soil as green manure showed potential for goosegrass control. However, “what exactly happens at the chemical level?” and “what are the compounds that are potentially responsible for the phytotoxic effects observed during those previous assays?” In a recent study, in vitro phytotoxicity bioassays and chemical analyses of milk vetch decomposition leachates were carried out to explore the relationship between the temporal phytotoxic effects and the dynamics of chemical composition. For that, milk vetch decomposition leachates with a decay time of 12 h, 9 days, 12 days, 15 days, and 18 days were analyzed for organic compounds by liquid chromatography. The main results were as follows: (1) three compounds with goosegrass suppression potential produced during the decomposed process, i.e., 4-ethylphenol, N-acrylimorpholine, and allyl isothiocyanate. 2-Hydroxyethyl acrylate was present in the 12-h decomposition leachates but was at its highest concentration of 127.1 µg ml−1 at 15 days. (2) The cultures were configured according to the four concentrations of goosegrass-resistant active substances measured in the 15-day decomposition leachate and, as with the 15-day decomposition leachate, the mixture cultures inhibited 100% of goosegrass germination at the high concentrations (≥ 30%), which suggests that these substances have goosegrass suppression potential. (3) The high total phenolic content (302.8–532.3 mg L−1), the total flavonoid content (8.4–72.1 mg L−1), and the reducing activity of the decomposition leachates for different decay times may explain why the incorporation of milk vetch into the soil did not lead to peroxidation of goosegrass in the previous study. (4) Finally, the changes in acid fraction and total content (1.9–4.2 mg ml−1) for different decay times explain the variations in pH of the decomposition leachates, which, when discussed in conjunction with previous studies, may lead to changes in soil nutrient effectiveness and consequently affect crop growth. This study can provide a reference for green weed control research.
研究领域:
植物学
·
农业科学
·
生态学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
COMPOSITES COMMUNICATIONS
·
2025
·
IF 7.7
Yina Zhuge,Haipei Ge,Fujuan Liu
Inspired by the extraordinary hierarchical architecture of penguins’ feathers, we have successfully prepared the biomimetic hierarchical fiber membranes. This was achieved by depositing rutile TiO 2 featuring nanocone structures on both the upper and lower surfaces of porous/membrane-pore-like fiber membranes using the chemical bath deposition method. When contrasted with conventional insulating materials (pure wool felt, twill cotton fabric, aramid 1313, polyethylene foam cotton and asbestos cloth), the novel hierarchical fiber membranes display outstanding thermal insulation capabilities (thermal conductivity = 0.0189 W m −1 K −1 ), remarkable high temperature resistance (more than 50 % of material remaining at 700 °C), excellent flame retardancy, and hydrophobicity (water contact angle exceeding 140°). These exceptional characteristics suggest that the hierarchically structured fiber membranes hold great promise for underwater thermal insulation applications, heralding the entry of the development of advanced thermal insulation materials into a new stage.
研究领域:
材料科学
·
生物仿生学
✓ 已验证
产品关联
DOI
高影响因子
POULTRY SCIENCE
·
2025
·
IF 4.2
Yujing Zhou,Jue Xu,Xiankang Fan,Qiang Xia,Changyu Zhou,Yangyang Hu,Hongbing Yan,Yangying Sun,Daodong Pan
In this study, the effect of pulsed electric field (PEF) pretreatment synergistic mixed bacterial agent ( Lactiplantibacillus plantarum PDD-1 and Lactococcus lactis subsp. lactis JCM5805) fermentation on the flavor and quality of air-dried goose meat and its molecular mechanism were investigated. The results showed that PEF combined with the mixed bacteria fermentation increased activity of monoamine oxidase (232.57 U/mg protein), decreased TBARS (0.3 mg/kg) and TVB-N (15.75 mg/100 g) content, and the hardness and chewiness in air-dried goose. The improvement in flavor due to the synergistic fermentation following PEF pretreatment was primarily attributed to increased levels of aromatic compounds such as Heptanal (green, sweet), (Z)-3-Hexenyl acetate (green fruity), 3-(methylthio)-propanol (onion, bouillion-like note), alongside decreased levels of off-flavor compounds like methyl phenylacetate and 5-methyl-2-acetylfuran, as determined by the e-nose and GC-IMS. Colony counts and high-throughput sequencing demonstrated that Lactococcus and Staphylococcu s predominated as the primary bacterial species, with different bioma onion, bouillion-like note rkers present in different air-dried goose groups. This is of strategic importance to develop the unique flavor and quality guarantee of air-dried goose.
研究领域:
食品科学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Yan Cheng,Yuanrong Zheng,Changyu Zhou,Siqiang Huang,Yangying Sun,Hongbing Yan,Jinxuan Cao,Daodong Pan,Qiang Xia
Compact microstructure of goose myofibrillar proteins (MP) negatively affects texture and flavor properties, as thick, large myofibers result in lower tenderness and limited capacity to absorb water and flavor compounds. This study proposes a strategy to regulate the MP microstructure and physicochemical qualities of conditioned meat by altering protonation signaling during pulsed electric field (PEF) processing. The results demonstrated that enhanced protonation during PEF significantly improved the meat quality. MP solubility increased by 4.91–9.03 %, while particle size decreased by 32.56–52.85 % compared to PEF alone or deprotonated conditions, attributed to protonation enhanced dissociation-depolymerization and unfolding of MP. Histological observations via the staining and electron microscopy revealed that enhanced protonation disrupted the muscle fiber arrangement and MP network. The tertiary structure of MP was altered, exposing internal hydrophobic regions and releasing volatile flavor compounds, which increased the headspace concentration of volatile compounds in goose meat during prolonged PEF treatment, thus helping enhance the complexity of meat flavor and the perception of characteristic aromas. These results demonstrated the dual effects of enhanced protonation and deprotonation during PEF treatments, which synergistically drove structural and functional changes in MP. The study validated the feasibility of altering protonation signaling during PEF to modulate meat processing characteristics.
研究领域:
食品科学
·
生物化学
✓ 已验证
DOI
PubMed
高影响因子
Food Bioscience
·
2025
·
IF 5.9
Yujing Zhou,Jue Xu,Xiankang Fan,Zihang Shi,Wei Wang,Ming Huang,Kaiyong Yao,Hongbing Yan,Changyu Zhou,Yangyang Hu,Daodong Pan
This research sought to find a novel way to control the biogenic amines (BAs) in air-dried goose through pulsed electric field (PEF) combined with mixed bacteria fermentation. The findings indicated that Lactiplantibacillus plantarum ( L . plantarum ) PDD-1 and Lactococcus lactis subsp. lactis ( L . lactis ) JCM5805 exhibited superior monoamine oxidase (MAO) and diamine oxidase (DAO) activities and robust degradation abilities for the four BAs (putrescine, cadaverine, histamine, tyramine) across various environmental conditions (temperature, NaCl concentration, and initial pH). Subsequent investigations revealed that the mixed bacteria ( L . plantarum PDD-1: L . lactis JCM5805 at a 1:1 ratio) demonstrated a more pronounced effect on BAs reduction compared to individual starter culture, particularly for putrescine and tyramine. The study results indicated that the optimal conditions for reducing BAs in air-dried goose was an electric field strength of 0.7 kV/cm applied for 150 s, followed by air-drying for 4 d. Consequently, the total BA content in the air-dried goose decreased from 15.98 ± 1.27 mg/100 g to 7.54 ± 0.04 mg/100 g. Targeted amino acid metabolomics further defined the degradation mechanism. This study is instructive for low BAs meat processing methods.
研究领域:
食品科学
·
微生物学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）