LWT-FOOD SCIENCE AND TECHNOLOGY
·
2021
·
IF 4.952
Lei Zhang,Lingli Zhong,Juying Wang,Yuqiang Zhao,Yiwei Zhang,Yitong Zheng,Weiliang Dong,Xianfeng Ye,Yan Huang,Zhoukun Li,Zhongli Cui
The process of raw starch conversion has attracted extensive attention in recent years due to its high energy conversion efficiency and saving of operational costs. In this study, extensive hydrolysis of raw starch by α-amylase AmyM from Corallococcus sp. EGB was investigated with robust substrate and products tolerance. AmyM showed broad substrate specificity toward raw starches with different types, and kept active and stable over a wide range of temperatures (30–70 °C) and pH values (5.0–9.0) and recycling. The V max values of AmyM towards wheat and corn raw starch were 1111 and 741 μmol/min/mg without depending on Ca 2+ , respectively. SEM analysis showed that AmyM hydrolysis results in the formation of porous and creviced surfaces around the starch granules peripheries. The cost-effective conversion of raw starch for the production of malto-oligosaccharides (MOSs) was identified. AmyM achieved high hydrolysis degree of 90% for 30% wheat and corn raw starch after 20 h at 45 °C with 5 U per mg of raw starch, resulting in formation of 70% MOSs and 30% dextrin. During this process, maltose (G2), maltotriose (G3) and maltotetraose (G4) were produced synchronously with G9 and G11, indicating the potential application in enzymatic conversion of raw starch under low temperature.
研究领域:
生物化学
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酶学
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食品科学
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生物技术
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DOI
高影响因子
CARBOHYDRATE POLYMERS
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2022
·
IF 10.723
Naixiang Xue,Yanli Wang,Xiaoxiao Li,Yuxiang Bai
Isomalto/malto-polysaccharides (IMMPs) with degree of polymerization (DP) 10–100 have novel potential applications, including enhanced solubility and anti-inflammatory. However, there are minimal synthetic methods for preparing IMMPs with a relatively higher DP, which is due to the lack of suitable molecular weight linear dextrins (I-LDs). The existing I-LDs preparation methods have disadvantages, such as low yield and uncontrollable molecular weight. Therefore, this study proposes a method for preparing soluble linear dextrins (S-LDs, M w = 2.1 kDa) by low-temperature retrogradation from debranched waxy corn starch (M w = 3.0 kDa). S-LDs reacted with 4,6-α-glucanotransferase GtfB-ΔN from Limosilactobacillus reuteri 121 to yield IMMPs with 12.3 kDa M w and 83.8% α1 → 6 linkages content. Process monitoring revealed the synthesis mechanism and a detailed reaction process. Finally, IMMPs were identified by enzyme fingerprinting as α1 → 6 chains with α1 → 4 fragments inlaid at the reducing, non-reducing end, and middle part. This study provides a new synthesis method and more structural information for IMMPs.
研究领域:
食品科学
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生物化学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
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2017
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IF 0.697
Xiaoting Zhang,Jiahua Niu,Yixin Yang,Peige Qin,Shufang Tian,Jinhua Zhu,Minghua Lu
In this work, a magnetic solid-phase extraction protocol followed with high-performance liquid chromatography analysis was developed for the determination of maltol and ethyl maltol in food samples. The Fe3O4 nanoparticles were prepared by one-step hydrothermal method and was used as adsorbent for clean and preconcentrated maltol and ethyl maltol in food samples. The as-prepared Fe3O4 nanoparticle was characterized by transmission electron microscope, Fourier transform infrared spectroscopy, X-ray diffraction, and vibrating sample magnetometer. The extraction conditions including the amount of material, extraction time, pH, temperature, and desorption solvents were investigated, respectively. Under the optimized conditions, the detection limits of 0.04 and 0.05 µg mL−1 could be achieved for maltol and ethyl maltol. The recoveries between 84.2 and 103.4% with RSDs lower than 2.30% were obtained for the analysis of spiked plum candy sample. The developed method was successfully applied to the analysis of real samples, such as juice, chocolate, and plum candy.
研究领域:
分析化学
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食品科学
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DOI
高影响因子
CARBOHYDRATE POLYMERS
·
2023
·
IF 11.2
Yixi Liu,Yazhen Wu,Hangyan Ji,Xiaoxiao Li,Zhengyu Jin,Birte Svensson,Yuxiang Bai
Isomalto/malto-polysaccharides (IMMPs) derived from malto-oligosaccharides such as maltoheptaose (G7) are elongated non-branched gluco-oligosaccharides produced by 4,6-α-glucanotransferase (GtfB). However, G7 is expensive and cumbersome to produce commercially. In this study, a cost-effective enzymatic process for IMMPs synthesis is developed that utilizes the combined action of cyclodextrinase from Palaeococcus pacificus (PpCD) and GtfB-ΔN from Limosilactobacillus reuteri 121 to convert β-cyclodextrin into IMMPs with a maximum yield (16.19 %, w/w). The purified IMMPs synthesized by simultaneous or sequential treatments, designated as IMMP-Sim and IMMP-Seq, possess relatively high contents of α-(1 → 6) glucosidic linkages. By controlling the release of G7 and smaller malto-oligosaccharides by PpCD, IMMP-Seq was obtained of DP varying from 12.9 to 29.5. Enzymatic fingerprinting revealed different linkage-type distribution of α-(1 → 6) linked segments with α-(1 → 4) segments embedded at the reducing end and middle part. The proportion of α-(1 → 6) segments containing the non-reducing end was 56.76 % for IMMP-Sim but 28.98 % for IMMP-Seq. Addition of G3 or G4 as specific acceptors resulted in IMMPs exhibiting low polydispersity . This procedure can be applied as a novel bioprocess that does not require costy high-purity malto-oligosaccharides and with control of the average DP of IMMPs by adjusting the substrate composition .
研究领域:
生物化学
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食品科学
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Jiayuan Wang,Laichuang Han,Maofang Teng,Qinghua Li,Jingwen Zhou,Jianghua Li,Guocheng Du,Guoqiang Zhang
Maltogenic amylase is a starch-hydrolyzing enzyme commonly used in bread baking and high-concentration maltose syrup production. However, low catalytic activity limits its industrial application. Improving catalytic activity based on molecular modification and directed evolution requires a High-Throughput Screening (HTS) method. In this study, a maltose gradient-induced (MaGI) biosensor was designed and applied for the directed evolution of maltogenic amylase AmyM, showing a good positive correlation between enzyme activity and fluorescence. The MaGI biosensor detected maltose and maltogenic amylase activity efficiently and specifically. Two mutants, Q440N and S442N/Q661L, were identified through the screening of 3000 mutants using the MaGI biosensor, showing a significant increase in catalytic activity of 35.56 % and 24.51 %, respectively, compared to the wild-type. Meanwhile, the t 1/2 of Q440N and S442N/Q661L at 60 °C increased by 58.53 % and 66.66 %, respectively. In industrial applications, the enhancement of catalytic activity and stability is conducive to improving production efficiency and reducing costs. MD simulation has found that when modifying multidomain enzymes, distal mutations can enhance catalytic activity. In conclusion, the developed MaGI biosensor is a promising tool for high-throughput and specific detection of maltose.
研究领域:
生物化学
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分子生物学
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工业酶学
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产品关联
DOI
PubMed
高影响因子
ENZYME AND MICROBIAL TECHNOLOGY
·
2021
·
IF 3.493
Mohammed Abdalla,Bo Jiang,Hinawi A.M. Hassanin,Luhua Zheng,Jingjing Chen
Maltodextrins (dextrins) are glucose chains normally produced by starch hydrolysis. Maltodextrins are characterized by their degree of polymerization (DP), which indicates the average number of glucose units per chain. Maltoheptaose (DP7), also known as amyloheptaose, is one of the maltodextrin mixtures widely used in foods, cosmetics, and pharmaceutical industries. Recently, the enzymatic synthesis of DP7 has attracted considerable attention, owing to its considerable advantages over chemical methods. In this work, we have designed a one-pot cascade reaction bio-synthesis starting from soluble starch to produce a specific degree of polymerization (DP7). The reaction system was catalyzed by cyclodextrin glucotransferase ( Ga CGT) from Gracilibacillus alcaliphilus SK51.001CGTase (transglycosylation/cyclization reaction) and cyclomaltodextrinase ( BsCD ) from Bacillus sphaericus E-244CDase (ring-opening reaction). The one-pot cascade reaction exhibited an optimum temperature of 30 °C and pH 7.0, and the addition of Ca 2+ enhanced the maltoheptaose production. The optimum enzyme units for the one-pot cascade reaction were 80 U/g of Ga CGT and 1 U/g of BsCD . However, the sequential addition of the enzymes exhibited a 5-fold higher conversion rate over simultaneous addition. The one-pot cascade reaction converted 30 g/L of soluble starch to 5.4 g/L of maltoheptaose in 1 h reaction time with a conversion rate of 16 %.
研究领域:
食品科学
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生物技术
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酶学
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产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2019
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IF 3.206
Xin Fu,Dongliang Zhu,Lan Huang,Xiangyang Yan,Shaomin Liu,Chenghui Wang
A highly sensitive and selective method for the simultaneous extraction and determination of trace vanillin , ethyl vanillin , maltol and ethyl maltol in beverage samples using magnetic solid-phase extraction coupled with high-performance liquid chromatography (HPLC) was developed. The novel superparamagnetic dummy template molecularly imprinted polymers (Fe 3 O 4 @MDMIP) were prepared by adopting a sol-gel approach using 3-hydroxy-2,6-bis(hydroxymethyl) -4-pyrone, whose structure was similar to that of the vanillin and maltol, as the dummy template for selective extraction of vanillin, ethyl vanillin, maltol and ethyl maltol. The obtained Fe 3 O 4 @MDMIP exhibits high uniformity and monodispersity. Besides, Fe 3 O 4 @MDMIP has an outstanding magnetic susceptibility (56.33 emu g −1 ) than common obtained samples. The maximal adsorption capacity (Q max ) of the resulting Fe 3 O 4 @MDMIP for the four food additives was up to 609.99 mg g −1 . The imprinting factors of the Fe 3 O 4 @MDMIP ranged from 2.07 to 2.71 for the four food additives, which indicated that the obtained adsorbents have high selectivity. Under the optimum experimental conditions, the Fe 3 O 4 @MDMIP was successfully applied to the magnetic solid-phase extraction of vanillin, ethyl vanillin, maltol and ethyl maltol followed by HPLC determination in beverage samples. Validation experiments indicated that the developed method presented good linearity (2.07–463 ng mL −1 ), satisfactory precision (RSD, 2.68–6.91%), and high recovery (91.2–107.1%). The limits of detection ranged from 44 to 201 ng L −1 , and the enrichment factors were in the range of 432–484. The results demonstrated great potential of the optimized method for sample preparation in routine analysis of trace food additives.
研究领域:
食品科学
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分析化学
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产品关联
DOI
高影响因子
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
·
2015
·Vol. 81
·期 6
·
IF 3.668
Zhoukun Li,Jiale Wu,Biying Zhang,Fei Wang,Xianfeng Ye,Yan Huang,Qiang Huang,Zhongli Cui
A novel α-amylase, AmyM, was purified from the culture supernatant of Corallococcus sp. strain EGB. AmyM is a maltohexaose-forming exoamylase with an apparent molecular mass of 43 kDa. Based on the results of matrix-assisted laser desorption ionization–time of flight mass spectrometry and peptide mass fingerprinting of AmyM and by comparison to the genome sequence of Corallococcus coralloides DSM 2259, the AmyM gene was identified and cloned into Escherichia coli. amyM encodes a secretory amylase with a predicted signal peptide of 23 amino acid residues, which showed no significant identity with known and functionally verified amylases. amyM was expressed in E. coli BL21(DE3) cells with a hexahistidine tag. The signal peptide efficiently induced the secretion of mature AmyM in E. coli. Recombinant AmyM (rAmyM) was purified by Ni-nitrilotriacetic acid (NTA) affinity chromatography, with a specific activity of up to 14,000 U/mg. rAmyM was optimally active at 50°C in Tris-HCl buffer (50 mM; pH 7.0) and stable at temperatures of <50°C. rAmyM was stable over a wide range of pH values (from pH 5.0 to 10.0) and highly tolerant to high concentrations of salts, detergents, and various organic solvents. Its activity toward starch was independent of calcium ions. The Km and V max of recombinant AmyM for soluble starch were 6.61 mg ml−1 and 44,301.5 μmol min−1 mg−1, respectively. End product analysis showed that maltohexaose accounted for 59.4% of the maltooligosaccharides produced. These characteristics indicate that AmyM has great potential in industrial applications.
研究领域:
生物化学
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酶学
·
微生物学
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产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Hui Dong,Linlin Zheng,Zhenlong Jiang,Tao Wang,Xinyue Li,Yangyang Ma,Zhu Chang,Xuechuan Lv,Xiaohan Gao,Maotian Xu,Yanli Zhou
Maltol (MA) is a common additive used in many fields, and its detection is of great importance to human health. Here, we synthesized a bimetallic MOF (CuNiMOF) based on graphite paper by a fast, simple, and green electrodeposition method. Then, we constructed a self-calibrated MOF to detect maltol. CuNiMOF was based on graphite paper by a fast, simple, and green electrodeposition method, and then constructed a self-calibrated flexible biosensor for the detection of maltol. In this work, we utilized the amperometric i-t curve (IT), cyclic voltammetry (CV), and multipotential step (STEP) methods to electrosynthesize three modified electrodes (Gp/CuNiMOF/IT, Gp/CuNiMOF/CV, Gp/CuNiMOF/STEP). The MA was characterized by differential pulse voltammetry electrochemical reactions on the electrode surface were investigated. The results showed that compared with the other two electrodeposition processes, the IT method was used to the sensor prepared by the IT method exhibits superior performance compared to the other two electrodeposition processes. The nonlinear response of the sensor to MA was in the concentration range of 0.5 ∼ 450 μM with a detection limit of 0.17 μM.
研究领域:
分析化学
·
材料科学
·
电化学传感器
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产品关联
DOI
高影响因子
Foods
·
2023
·Vol. 12
·期 18
·3487
·
IF 5.2
Jihong Wang,Lei Zhang,Peiwen Wang,Jinhui Lei,Lingli Zhong,Lei Zhan,Xianfeng Ye,Yan Huang,Xue Luo,Zhongli Cui,Zhoukun Li
Malto-oligosaccharides (MOSs) from starch conversion is advantageous for food and pharmaceutical applications. In this study, an efficient malto-oligosaccharide-forming α-amylase AmyCf was identified from myxobacterCystobactersp. strain CF23. AmyCf is composed of 417 amino acids with N-terminal 41 amino acids as the signal peptide, and conserved glycoside hydrolase family 13 (GH13) catalytic module and predicted C-terminal domain with β-sheet structure are also identified. Phylogenetic and functional analysis demonstrated that AmyCf is a novel member of GH13_6 subfamily. The special activity of AmyCf toward soluble starch and raw wheat starch is 9249 U/mg and 11 U/mg, respectively. AmyCf has broad substrate specificity toward different types of starches without requiring Ca2+. Under ideal circumstances of 60 °C and pH 7.0, AmyCf hydrolyzes gelatinized starch into maltose and maltotriose and maltotetraose as the main hydrolytic products with more than 80% purity, while maltose and maltotriose are mainly produced from the hydrolysis of raw wheat starch with more than 95% purity. The potential applicability of AmyCf in starch processing is highlighted by its capacity to convert gelatinized starch and raw starch granules into MOSs. This enzymatic conversion technique shows promise for the low-temperature enzymatic conversion of raw starch.Keywords:Malto-oligosaccharides;Cystobacter;gelatinized starch;enzymatic conversion;raw starch
研究领域:
生物化学
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食品科学
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酶学
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DOI
PubMed
高影响因子
CARBOHYDRATE POLYMERS
·
2024
·
IF 10.7
Lingli Zhong,Peiwen Wang,Min Jiang,Yitong Zheng,Xiaofan Xu,Xianfeng Ye,Yan Huang,Yanling Ji,Zhongli Cui,Zhoukun Li
As attractive functional ingredients, maltooligosaccharides (MOS) are typically prepared by controlled enzymatic hydrolysis of starch. However, the random attack mode of amylase often leads to discrete product distribution, thereby reducing yields and purities. In this study, a novel glycoside hydrolase family 13 amylase AmyEs from marine myxobacteria Enhygromyxa salina was identified efficient maltohexaose (G6)-forming ability (40 %, w/w). By deciphering external chain length, we found that the high density of α-1,6-branching points benefits the G6 formation of AmyEs with high purity (71–82 %), indicating the substrate selectivity of AmyEs toward high-branched starch. Based on this, asynchronous conversion strategy was designed to enhance specific MOS yield from corn starch by exploiting branching enzymes and AmyEs, and the purity and yield of G6 respectively increased by 9.5 % and 5 % compared to single AmyEs treatment. Our results demonstrate that combinatorial catalysis of MOS-forming amylases and branching enzymes provides a favorable industrial preparation of specific MOS.
研究领域:
生物化学
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酶学
·
食品科学
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微生物学
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DOI
PubMed
高影响因子
Toxins
·
2023
·Vol. 15
·期 3
·209
·
IF 4.2
Yingyue Zhang,Fengyan Kuang,Chunyao Liu,Kai Ma,Tianyu Liu,Meijuan Zhao,Guangping Lv,He Huang
Edible and medicinal plants (EMPs) are widely used but are easily infected by harmful fungi which produce mycotoxins. Herein, 127 samples from 11 provinces were collected to investigate 15 mycotoxins based on geographic, demographic, processing, and risk characteristics. A total of 13 mycotoxins were detected, and aflatoxin B1(0.56~97.00 μg/kg), deoxynivalenol (9.41~1570.35 μg/kg), fumonisin B1(8.25~1875.77 μg/kg), fumonisin B2(2.74~543.01 μg/kg), ochratoxin A (0.62~19.30 μg/kg), and zearalenone (1.64~2376.58 μg/kg) occurred more frequently. Mycotoxin levels and species were significantly different by region, types of EMPs, and method of processing. The margin of exposure (MOE) values was well below the safe MOE (10,000). AFB1exposure from Coix seed and malt consumption in China was of high health concern. The hazard Index (HI) method showed the range of 113.15~130.73% for malt, indicating a public health concern. In conclusion, EMPs should be concerned because of the cumulative effects of co-occurred mycotoxins, and safety management strategies should be developed in follow-up studies.Keywords:mycotoxins;coix seed;lilii bulbus;malt;lotus seed;risk assessmentKey Contribution:This study investigated the co-occurrence of mycotoxins in EMPs from China, and indicated a high risk of mycotoxin exposure from Coix Seed and Malt. Mycotoxin contamination in EMPs varied significantly, and it may require more explicit limits for specific EMPs.
研究领域:
食品安全
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植物学
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公共卫生
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）