ACS Omega
·
2021
·
IF 3.512
Daihua Hu,Xu Yang,Chingyuan Hu,Zili Feng,Wang Chen,Hanmeng Shi
Vitamin D deficiency is a severe worldwide health issue. Edible mushrooms are an excellent vitamin D2 source and have gained popularity worldwide as a nutritional food. The objective of this study was to investigate the conversion efficiency of ergosterol to vitamin D2 in Agaricus bisporus and Cordyceps militaris mushrooms under ultraviolet (UV) irradiation directly through dry powder or in ethanol suspension (1:20 g/mL, solid to liquid ratio). Several parameters of UV irradiation conditions such as the material form (dry powder or dry powder in ethanol suspension), exposure time (30, 60, or 120 min), wavelength type (UV-C, UV-B, or UV-A), wavelength combination (UV-C plus UV-B, UV-C plus UV-A, UV-B plus UV-A, or UV-C plus UV-B plus UV-A), and wavelength sequence (UV-C → UV-B, UV-C → UV-A, UV-B → UV-A, or UV-C → UV-B → UV-A), were optimized. Under the optimal UV irradiation conditions (dry powder in ethanol suspension irradiated with UV-C at 40 cm for 120 min), vitamin D2 concentrations increased from not detectable to 72 μg/g (dw) in the A. bisporus dry powder and 1104 μg/g (dw) (about 15-fold increase) in the ethanol suspension. After UV irradiation, the vitamin D2 concentration increased from undetectable to 57 μg/g (dw) in the C. militaris dry powder. In contrast, UV irradiation increased the concentration to 877 μg/g (dw) (about 15-fold higher) in the ethanol suspension. Comparison of the effect of various wavelength combinations showed that UV-C irradiation is more effective than UV-A or UV-B. Furthermore, when irradiated by UV-C at a 40 cm irradiation distance in the ethanol suspension, the increase in vitamin D2 in A. bisporus and C. militaris mushrooms was time- or dose-dependent. The conversion rate of vitamin D2 was low to undetectable under dry powder irradiation, but its ergosterol loss rate was higher than in ethanol suspension irradiation. The ergosterol loss rate in dry C. militaris mushrooms was higher than in the dry A. bisporus mushroom powder. Ultraviolet irradiation in ethanol suspension could greatly increase the vitamin D2 concentration than directly on the dry powder and thus make edible mushrooms more practical as a natural vitamin D source for consumers after entirely removing the ethanol.
研究领域:
营养学
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食品科学
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生物化学
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产品关联
DOI
PubMed
高影响因子
Journal of Fungi
·
2021
·Vol. 7
·期 6
·432
·
IF 5.816
Qiuli OuYang,Yangmei Liu,Okwong Reymick Oketch,Miaoling Zhang,Xingfeng Shao,Nengguo Tao
Ergosterol (ERG) is a potential target for the development of antifungal agents againstPenicillium digitatum, the pathogen of green mold in citrus fruits. This study examined the mechanism by which citronellal, a typical terpenoid ofCymbopogon nardusessential oil, acts on ergosterol to exhibit its antifungal activity againstP. digitatum. We previously reported that citronellal inhibited the growth ofP. digitatumwith minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) of 1.36 and 2.72 mg/mL, respectively. In citronellal-treated cells, the membrane integrity and ergosterol contents significantly decreased, whereas lanosterol, which serves as a precursor for ergosterol biosynthesis, massively accumulated. Addition of 150 mg/L of exogenous ergosterol decreased the inhibitory rate of citronellal, restoring the ergosterol content and hence the membrane structure to normal levels, and triggered expression of nearly all ERG genes. Based on our findings, we deduce that citronellal damages the cell membrane integrity ofP. digitatumby down-regulating the ERG genes responsible for conversion of lanosterol to ergosterol, the key downregulated gene beingERG3, due to the observed accumulation of ergosta-7,22-dienol.Keywords:citrus;pathogen;ergosterol;ERG genes;cell membrane integrity
研究领域:
微生物学
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植物病理学
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植物化学
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DOI
PubMed
高影响因子
MOLECULAR BIOLOGY REPORTS
·
2012
·Vol. 39
·期 10
·9493-9508
·
IF 2.929
Cheng Zhengjun
This study was designed to examine the interactions of ergosterol with bovine serum albumin (BSA) and human serum albumin (HSA) under physiological conditions with the drug concentrations in the range of 2.99–105.88 μM and the concentration of proteins was fixed at 5.0 μM. The analysis of emission spectra quenching at different temperatures revealed that the quenching mechanism of HSA/BSA by ergosterol was the static quenching. The number of binding sites n and the binding constants K were obtained at various temperatures. The distance r between ergosterol and HSA/BSA was evaluated according to Föster non-radioactive energy transfer theory. The results of synchronous fluorescence, 3D fluorescence, FT-IR, CD and UV–Vis absorption spectra showed that the conformations of HSA/BSA altered in the presence of ergosterol. The thermodynamic parameters, free energy change (Δ G ), enthalpy change (Δ H ) and entropy change (Δ S ) for BSA–ergosterol and HSA–ergosterol systems were calculated by the van’t Hoff equation and discussed. Besides, with the aid of three site markers (for example, phenylbutazone, ibuprofen and digitoxin), we have reported that ergosterol primarily binds to the tryptophan residues of BSA/HSA within site I (subdomain II A).
研究领域:
生物化学
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药物化学
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产品关联
DOI
PubMed
高影响因子
ACS Omega
·
2020
·
IF 2.87
Daihua Hu,Wang Chen,Xinsheng Li,Tianli Yue,Zhijian Zhang,Zili Feng,Cuili Li,Xing Bu,Qing X. Li,Ching Y. Hu,LiuDi Li
Vitamin D deficiency is a serious global health problem. Edible mushrooms are a good source of vitamin D for human health. The objective of this experiment was to investigate the efficiency of converting its precursor ergosterol to vitamin D2 in shiitake mushroom (Lentinus edodes) and oyster mushroom (Pleurotus ostreatus) powder in ethanol suspension under ultraviolet (UV) irradiation. UV irradiation conditions were optimized for several parameters, such as material form, wavelength, wavelength combination, and exposure time. Under the optimal conditions, UV irradiation increased the concentrations of vitamin D2 from undetectable to 40.59 ± 1.16 μg/g (dw) in dry shiitake mushroom powder and to 677.28 ± 40.42 μg/g (dw) (an approximately 16.69-fold increase) in ethanol suspension. The concentration of vitamin D2 increased from undetectable to 23.71 ± 5.72 μg/g (dw) in the dry oyster mushroom powder upon UV irradiation, whereas UV irradiation increased the concentration to 275.32 ± 48.45 μg/g (dw) (an approximately 11.61-fold increase) in the ethanol suspension. Comparing the effects of varying combinations of wavelengths showed that irradiation with UV-A, UV-C, or a combination of both is more effective than UV-B irradiation. In addition, the increase in vitamin D2 in shiitake mushrooms irradiated by UV-C was time-dependent, that is, dose-dependent. Nevertheless, the increase rates decreased with time. The concentration of ergosterol decreased with the increase in vitamin D2, but ergosterol was only partially converted to vitamin D2, whereas most of the ergosterol was probably UV-degraded. Exposure to ultraviolet light in ethanol suspension offers an effective way to increase the concentration of vitamin D2 and thus improve the nutritional value of edible mushrooms, as well as make them more functional as a source of vitamin D to improve the consumer health.
研究领域:
食品科学
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营养学
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真菌学
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DOI
PubMed
高影响因子
DEVELOPMENTAL CELL
·
2024
·
IF 10.7
Rongrong He,Ziwei Lv,Yinan Li,Shuchao Ren,Jiaqi Cao,Jun Zhu,Xinrong Zhang,Huimin Wu,Lihao Wan,Ji Tang,Shutong Xu,Xiao-Lin Chen,Zhipeng Zhou
Ergosterols are essential components of fungal plasma membranes. Inhibitors targeting ergosterol biosynthesis (ERG) genes are critical for controlling fungal pathogens, including Magnaporthe oryzae , the fungus that causes rice blast. However, the translational mechanisms governing ERG gene expression remain largely unexplored. Here, we show that the Trm6/Trm61 complex catalyzes dynamic N 1 -methyladenosine at position 58 (m 1 A58) in 51 transfer RNAs (tRNAs) of M. oryzae , significantly influencing translation at both the initiation and elongation stages. Notably, tRNA m 1 A58 promotes elongation speed at most cognate codons mainly by enhancing eEF1-tRNA binding rather than affecting tRNA abundance or charging. The absence of m 1 A58 leads to substantial decreases in the translation of ERG genes, ergosterol production, and, consequently, fungal virulence. Simultaneously targeting the Trm6/Trm61 complex and the ergosterol biosynthesis pathway markedly improves rice blast control. Our findings demonstrate an important role of m 1 A58-mediated translational regulation in ergosterol production and fungal infection, offering a potential strategy for fungicide development.
研究领域:
分子生物学
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微生物学
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植物病理学
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产品关联
DOI
PubMed
高影响因子
MEDICINE
·
2018
·
IF 2.028
Meijia Wu,Ting Huang,Juan Wang,Ping Chen,Wanwan Mi,Yuanyuan Ying,Hangli Wang,Dandan Zhao,Shengwu Huang
Supplemental Digital Content is available in the text
研究领域:
药学
·
肿瘤学
·
生物医学工程
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DOI
PubMed
高影响因子
Frontiers in Microbiology
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2024
·
IF 4
Yang Jiao,Yu Zheng,Shiqin Wu,Li Zhou,Hua Jiang,Yuanhong Li,Fuxing Lin
Botrytis cinerea may cause gray mold in fruits and vegetables. Paeonol, an active component of traditional Chinese medicine, could suppress various microbial growth. However, reports on its effect on B. cinerea have not yet been documented. In this paper, we demonstrated that paeonol completely inhibited B. cinerea growth at 250 mg/L, corroborated by the observation of irregular morphological alterations in B. cinerea exposed to paeonol. Notably, the investigation of the operating mechanism revealed that paeonol induced cell death by disrupting the cell membrane, potentially mediated by the interaction between paeonol and ergosterol from the membrane. Further studies indicated that paeonol decreased ergosterol content and the expression of certain genes involved in ergosterol biosynthesis was significantly downregulated. In addition, paeonol treatment reduced the gray mold of cherry tomatoes. Meanwhile, compared to the control treatment, paeonol treatment could reduce weight loss and maintain higher contents of total soluble solid (TSS) and ascorbic acid, leading to a higher quality of the stored cherry tomato. Together, the data indicate that paeonol was effective as an alternative agent targeting disrupting the cell membrane to control gray mold and prolong the shelf life of cherry tomatoes, suggesting that paeonol could be used as a natural antifungal compound during postharvest storage.
研究领域:
植物病理学
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食品科学
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药理学
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真菌学
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DOI
PubMed
高影响因子
CLINICAL SCIENCE
·
2019
·Vol. 133
·期 13
·1523-1536
·
IF 5.237
Sun Xiao,Feng Xiuli,Zheng Dandan,Li Ang,Li Chunyan,Li Siying,Zhao Zhongxi
Cigarette smoke (CS) is the major cause of chronic obstructive pulmonary disease (COPD). CS heightens inflammation, oxidative stress and apoptosis. Ergosterol is the main bioactive ingredient in Cordyceps sinensis (C. sinensis), a traditional medicinal herb for various diseases. The objective of this work was to investigate the effects of ergosterol on anti-inflammatory and antioxidative stress as well as anti-apoptosis in a cigarette smoke extract (CSE)-induced COPD model both in vitro and in vivo. Our results demonstrate that CSE induced inflammatory and oxidative stress and apoptosis with the involvement of the Bcl-2 family proteins via the nuclear factor kappa B (NF-κB)/p65 pathway in both 16HBE cells and Balb/c mice. CSE induced epithelial cell death and increased the expression of nitric oxide (NO), interleukin-6 (IL-6), tumor necrosis factor α (TNF-α), malondialdehyde (MAD) and the apoptosis-related proteins cleaved caspase 3/7/9 and cleaved-poly-(ADP)-ribose polymerase (PARP) both in vitro and in vivo, whereas decreased the levels of superoxide dismutase (SOD) and catalase (CAT). Treatment of 16HBE cells and Balb/c mice with ergosterol inhibited CSE-induced inflammatory and oxidative stress and apoptosis by inhibiting the activation of NF-κB/p65. Ergosterol suppressed apoptosis by inhibiting the expression of the apoptosis-related proteins both in vitro and in vivo. Moreover, the usage of QNZ (an inhibitor of NF-κB) also partly demonstrated that NF-κB/p65 pathway was involved in the ergosterol protective progress. These results show that ergosterol suppressed COPD inflammatory and oxidative stress and apoptosis through the NF-κB/p65 pathway, suggesting that ergosterol may be partially responsible for the therapeutic effects of cultured C. sinensis on COPD patients.
研究领域:
药理学
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呼吸医学
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细胞生物学
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产品关联
DOI
PubMed
高影响因子
PEST MANAGEMENT SCIENCE
·
2023
·
IF 4.1
Tianze Li,Min Lv,Houpeng Wen,Jiawei Du,Zhen Wang,Shaoyong Zhang,Hui Xu
Background Phytopathogenic fungi can cause a direct loss in economic value of agriculture. Especially Valsa mali Miyabe et Yamada, a devastating phytopathogenic disease especially threatening global apple production, is very difficult to control and manage. To discover new potential antifungal agents, a series of thiosemicarbazone derivatives of 3-acetyl- N -benzylindoles were prepared. Their antifungal activities were first tested against six typically phytopathogenic fungi including Curvularia lunata , Valsa mali , Alternaria alternate , Fusarium graminearum , Botrytis cinerea and Fusarium solani . Then their mechanism of action against V. mali was investigated. Results Derivatives displayed potent antifungal activity against V. mali . Notably, 3-acetyl- N -benzylindole thiosemicarbazone (IV-1: EC 50 : 0.59 μ g mL −1 ), whose activity was comparable to that of a commercial fungicide carbendazim (EC 50 : 0.33 μ g mL −1 ), showed greater than 98-fold antifungal activity of the precursor indole. Moreover, compound IV-1 displayed good protective and therapeutic effects on apple Valsa canker disease. By scanning electron microscope (SEM) and RNA-Seq analysis, it was demonstrated that compound IV-1 can destroy the hyphal structure and regulate the homeostasis of metabolism of V. mali via the ergosterol biosynthesis and autophagy pathways. Conclusion 3-Acetyl- N -(un)substituted benzylindoles thiosemicarbazones (IV-1–IV-5) can be studied as leads for further structural modification as antifungal agents against V. mali . Particularly, these ergosterol biosynthesis and autophagy pathways can be used as target receptors for design of novel green pesticides for management of congeneric phytopathogenic fungi. © 2023 Society of Chemical Industry.
研究领域:
农业科学
·
植物病理学
·
化学
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DOI
PubMed
高影响因子
PROCESS BIOCHEMISTRY
·
2016
·
IF 2.529
Fang Fang,Bingye Dai,Guang Zhao,Hua Zhao,Chao Sun,Hui Liu,Mo Xian
Considering of the possible application of fatty acids secretion in industrial production process for reducing extraction costs, the effects of enhanced fatty acids secretion by knocking out acyl-CoA synthetases on the global regulation, cellular metabolism were investigated through integrated omics analysis. Firstly, functional category analysis of differential expression genes showed that most of the genes were associated with the cell cycle and biology process such as cellular component biogenesis, cell wall organization and so on. Secondly, the decreased mRNA levels of Tdh, Eno and Pgk1 in glycolysis in faa mutant YB526 were confirmed by the differential protein analysis. As described in previous reports, the decreased protein levels of Tdh, Eno and Pgk1 were resulted from the increase of 14-3-3 protein activity. However, the increased level of 14-3-3 proteins could not improve the biosynthesis of ergosterol in the acyl-CoA deficiency strain YB526 due to the toxic effects of fatty acids. Thirdly, the decrease of ergosterol and ceramide contents in cell envelopes can improve the permeation of membrane and fatty acids secretion in faa mutant YB526. This comprehensive omics analysis could be helpful in the rational design of robust yeasts for the production of free fatty acids or other biobased chemicals.
研究领域:
微生物学
·
分子生物学
·
系统生物学
·
工业生物技术
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产品关联
DOI
高影响因子
MOLECULES
·
2024
·Vol. 29
·期 18
·4375
·
IF 4.2
Ran Luo,Haoyi Zhao,Siqi Deng,Jiale Wu,Haijun Wang,Xiaoshan Guo,Cuicui Han,Wenkang Ren,Yinglong Han,Jianwen Zhou,Yu Lin,Ming Bu
In this study, novel ergosterol peroxide (EP) derivatives were synthesized and evaluated to assess their antiproliferative activity against four human cancer cell lines (A549, HepG2, MCF-7, and MDA-MB-231). Compound3gexhibited the most potent antiproliferative activity, with an IC50value of 3.20 µM against MDA-MB-231. This value was 5.4-fold higher than that of the parental EP. Bioassay optimization further identified3gas a novel glutaminase 1 (GLS1) inhibitor (IC50= 3.77 µM). In MDA-MB-231 cells,3greduced the cellular glutamate levels by blocking the glutamine hydrolysis pathway, which triggered reactive oxygen species production and induced caspase-dependent apoptosis. Molecular docking indicated that3ginteracts with the reaction site of the variable binding pocket by forming multiple interactions with GLS1. In a mouse model of breast cancer,3gshowed remarkable therapeutic effects at a dose of 50 mg/kg, with no apparent toxicity. Based on these results,3gcould be further evaluated as a novel GLS1 inhibitor for triple-negative breast cancer (TNBC) therapy.
研究领域:
肿瘤学
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药理学
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分子生物学
·
癌症研究
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DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2024
·
IF 5.7
Jing Liu,Hao Chen,Yan Lv,Huixiang Wu,Li-jun Yang,Jiahui Zhang,Jianying Huang,Wenjie Wang
Aspergillus flavus contamination in agriculture and food industries poses threats to human health, leading to a requirement of a safe and effective method to control fungal contamination. Chitosan-based nitrogen-containing derivatives have attracted much attention due to their safety and enhanced antimicrobial applications. Herein, a new benzimidazole-grafted chitosan (BAC) was synthesized by linking the chitosan (CS) with a simple benzimidazole compound, 2-benzimidazolepropionic acid (BA). The characterization of BAC was confirmed by Fourier transform infrared (FTIR) spectroscopy and nuclear magnetic resonance spectroscopy (1H and 13C NMR). Then, the efficiency of BAC against A. flavus ACCC 32656 was investigated in terms of spore germination, mycelial growth, and aflatoxin production. BAC showed a much better antifungal effect than CS and BA. The minimum inhibitory concentration (MIC) value was 1.25 mg/mL for BAC, while the highest solubility of CS (16.0 mg/mL) or BA (4.0 mg/mL) could not completely inhibit the growth of A. flavus. Furthermore, results showed that BAC inhibited spore germination and elongation by affecting ergosterol biosynthesis and the cell membrane integrity, leading to the permeabilization of the plasma membrane and leakage of intracellular content. The production of aflatoxin was also inhibited when treated with BAC. These findings indicate that benzimidazole-derived natural CS has the potential to be used as an ideal antifungal agent for food preservation.
研究领域:
材料科学
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微生物学
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食品科学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）