BIORESOURCE TECHNOLOGY
·
2015
·
IF 4.494
Guanyi Wang,Di Huang,Yong Li,Jianping Wen,Xiaoqiang Jia
In this work, wheat bran (WB) was utilized as feedstock to synthesize fumaric acid by Rhizopus oryzae . Firstly, the pretreatment process of WB by dilute sulfuric acid hydrolysis undertaken at 100 °C for 30 min offered the best performance for fumaric acid production. Subsequently, through optimizing the seed culture medium, a suitable morphology (0.55 mm pellets diameter) of R. oryzae was obtained. Furthermore, a metabolic-based approach was developed to profile the differences of intracellular metabolites concentration of R. oryzae between xylose (the abundant sugar in wheat bran hydrolysate (WBH)) and glucose metabolism. The xylitol, sedoheptulose 7-phosphate, ribulose 5-phosphate, glucose 6-phosphate, proline and serine were responsible for fumaric acid biosynthesis limitation in xylose fermentation. Consequently, regulation strategies were proposed, leading to a 149% increase in titer (up to 15.4 g/L). Finally, by combinatorial regulation strategies the highest production was 20.2 g/L from WBH, 477% higher than that of initial medium.
研究领域:
生物工程
·
代谢生物学
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DOI
PubMed
高影响因子
Foods
·
2023
·Vol. 12
·期 12
·2424
·
IF 5.2
Yang Zhao,Xinrui Li,Shuo Guo,Jingwen Xu,Yan Cui,Mingzhu Zheng,Jingsheng Liu
Xylanases are the preferred enzymes for the extracting of oligosaccharides from wheat bran. However, free xylanases have poor stability and are difficult to reuse, which limit their industrial application. In the present study, we covalently immobilized free maleic anhydride-modified xylanase (FMA-XY) to improve its reusability and stability. The immobilized maleic anhydride-modified xylanase (IMA-XY) exhibited better stability compared with the free enzyme. After six repeated uses, 52.24% of the activity of the immobilized enzyme remained. The wheat bran oligosaccharides extracted using IMA-XY were mainly xylopentoses, xylohexoses, and xyloheptoses, which were the β-configurational units and α-configurational units of xylose. The oligosaccharides also exhibited good antioxidant properties. The results indicated that FMA-XY can easily be recycled and can remain stable after immobilization; therefore, it has good prospects for future industrial applications.Keywords:immobilized enzymes;kinetics;thermodynamics;wheat bran;oligosaccharide
研究领域:
生物化学
·
酶学
·
食品科学
·
热力学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2015
·
IF 2.912
Jia Guo,Yuan-Yuan Bian,Ke-Xue Zhu,Xiao-Na Guo,Wei Peng,Hui-Ming Zhou
Wheat bran contains a significant amount of the anti-nutritional factor phytate. This study is the first to explore the effectiveness of activating endogenous phytase and further reducing phytate content through resulting programmed cell death (PCD). Effects of solid–liquid ratio (1:1, 1:2, 1:3, and 1:6), incubation temperature (4, 20, 38, 55, and 70 °C), metal ions (Na+, K+, Ca2+, and Mg2+), gibberellin concentration (0, 5, 50, 500, 2000, and 5000 mg/L), hydrogen peroxide concentration (0, 0.2, 0.4, 0.6, 0.8, and 1.0%), and incubation time (30, 80, 180, and 360 min) on activation of endogenous phytase activity and phytate degradation in wheat bran samples are discussed in this study. It was found that when the wheat bran was incubated with distilled water at 55 °C for 80 min, its endogenous phytase activity was dramatically increased 4-fold from 12.96 to 53.54 FTU/g, whereas the phytate content was reduced by about 70% from 45.20 to 13.52 mg/g. By comparison of photomicrographs of raw wheat bran sample and sample incubated with distilled water for 360 min at 55 °C, a conclusion could be drawn that PCD in aleurone cells had occurred.
研究领域:
食品科学
·
植物生理学
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DOI
高影响因子
Food & Function
·
2016
·Vol. 7
·期 8
·3524-3530
·
IF 2.686
Yuhui Li,Kaiyong Liu,Jie Shen,Yehao Liu
Environmental exposure to pollutants such as heavy metals is responsible for various altered physiological functions that are detrimental to health. Cadmium (Cd) is one of the most representative toxic, non-essential elements that can contaminate food and water. This study evaluated the protective effects of wheat bran, a selected prebiotic with good Cd binding ability, against Cd toxicity in mice. Thirty mice were divided into control and therapeutic groups. The control group was fed a low dietary fiber diet with no Cd for 8 weeks. In the therapeutic group, 100 mg L−1 Cd was administered along with a diet of 10% wheat bran. First, the binding ability of wheat bran for Cd was measured along with the protection of cell growth in vitro by wheat bran. Second, the effectiveness of wheat bran in preventing Cd from entering mice organs was evaluated. Finally, restoration of gut microbiota from alterations caused by Cd was investigated. The results showed that wheat bran could bind most of the Cd ions and restore the growth rate of cells in vitro. The wheat bran treatment group showed that the bran effectively prevented Cd from entering mice organs. In addition, the bacterial community structure was restored because the toxicity of Cd to gut microbiota was eliminated by the wheat bran. Our results suggest that wheat bran is more effective against chronic Cd toxicity than traditional treatments because of its special physiological functions, and it can be considered as a new dietary, therapeutic strategy against Cd toxicity.
研究领域:
环境科学
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营养学
·
微生物学
·
毒理学
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DOI
PubMed
高影响因子
CHEMICAL PHYSICS LETTERS
·
2018
·
IF 1.686
Wei-Xing Zhang,Lu Lai,Ping Mei,Yan Li,Yu-Hang Li,Yi Liu
A natural biosorbent prepared from wheat bran (WB) and multiple quaternary ammonium salts (MQAS) was used to remove dye (AR-18) from aqueous solution. The adsorption kinetics and thermodynamic mechanism of the adsorption of AR-18 on MQAS-WB were also examined. Results reveal that adsorption isotherm data can be well described by Langmuir model. The calculated thermodynamic parameters indicate that the adsorption is exothermic and spontaneous. The adsorption kinetics follows the pseudo-second-order model. MQAS-WB shows potential for large-scale wastewater treatment. Dynamic adsorption studies reveal that the dye removal efficiency of the packing column of MQAS-WB is higher than that of raw WB.
研究领域:
环境科学
·
化学工程
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF CEREAL SCIENCE
·
2015
·
IF 2.094
Jia Guo,Yuan-Yuan Bian,Ke-Xue Zhu,Xiao-Na Guo,Wei Peng,Hui-Ming Zhou
Wheat bran is good for human health due to the abundance of dietary fiber. However, it also contains much phytate which has been well documented as an anti-nutritional factor. This study is the first to explore the effectiveness of reducing phytate content in wheat bran by directly removing the aleurone cell content utilizing different roller mills (smooth roller, coarse smooth roller and teeth roller) and different clean-up methods (brushing and ultrasonic washing). Through analysis of phytate content in wheat bran obtained from different mill systems, the best raw material was found to be obtained from the 5B system. By investigating various roller types and clean-up methods, the optimal technique was found to use a teeth roller mill combined with ultrasonic washing, which could reduce the phytate content by the greatest amount: the reduction of phytate content achieved 62.98% (from 26.80 mg/g to 9.92 mg/g) in wheat bran. The optical microscope observation indicated that ultrasonic washing could wash the aleurone cell content out from broken aleurone cells which were destroyed by the teeth roller mill.
研究领域:
食品科学
·
营养学
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产品关联
DOI
高影响因子
CEREAL CHEMISTRY
·
2021
·Vol. 99
·期 3
·582-592
·
IF 1.984
Fan Wu,Fulin Tian,Zhou Jin,Jing Liu,Weiping Jin,Xuan Chen,Zhan Wang,Xiwu Jia,Benguo Liu,Lingyi Liu,Wangyang Shen
Background and objectives Pure wheat bran and wheat germ are the by-products of sieved flour. Pure wheat bran contains a substantial amount of dietary fiber, vitamins B and D, and other nutrients; lipases in the wheat germ induce lipid oxidation and break down vitamins. The present study used heat treatment to stabilize the wheat germ and then compounded it with the extruded pure wheat bran and compared it with the extruded bran. The antioxidant capacity and soluble polyphenols of the compounded wheat bran and extruded wheat bran were subsequently measured, to select a method to improve the antioxidant capacity of wheat bran. Findings The optimized heating temperature of wheat germ was 110°C. The half-maximal inhibitory concentration value of the total antioxidant capacity showed that the compounded wheat bran had a higher antioxidant capacity than the extruded wheat bran. It was hypothesized that the operations of extrusion and heat treatment increased the antioxidant capacity of compounded wheat bran. In addition, after the extrusion treatment, a variety of flavonoid glycosides in the wheat bran was released while the content of various phenolic acid types was decreased. However, the content of the phenolic acid types and flavonoids in the compounded wheat bran was increased. Hence, compounded wheat bran is more suitable for producing functional foods. Conclusion The results of this study revealed that extruded pure wheat bran and heat-treated wheat germ can effectively increase the antioxidant capacity and the content of the phenolic acid types and flavonoids; hence, it is more suitable for producing functional foods. Significance and novelty This study developed a fast and inexpensive detection method that uses a combination of heat and extrude to process compounded wheat bran, which provides the optimal process method for extending the yield and shelf life of wheat bran products.
研究领域:
食品科学
·
营养学
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产品关联
DOI
高影响因子
JOURNAL OF CEREAL SCIENCE
·
2025
·
IF 3.7
Yuan Guo,Baoyi Wang,Xiaona Zhai,Liang Liang,Jianxiong Hao,Haisheng Pei,Yuanyuan Li
The bond agglomeration of wheat bran (WB) powder with superfine grinding (SG) time extension is a technological barrier to its application in wheat flour. Gluten protein is considered to be the main component contributing to whole wheat flour quality. The effect of SG combined with glutenin treatment on the physicochemical and structural properties of WB powder was investigated in this study. The results indicated that when the crushing time reached 30 min, the combination of SG and glutenin treatment significantly reduced the D v 50 value to 15.60 μm and the span value to 2.57, while increasing the soluble dietary fiber (SDF) content to 8.64% of WB compared to the use of either SG or glutenin treatment individually. Besides, the combination of SG and glutenin in comparison to SG alone facilitated the destruction of crystalline regions and the regulation of internal functional groups of cellulose and hemicellulose, resulting in a reduction of the crystallinity from 28.65% to 22.34%. Furthermore, treatment with both SG and glutenin augmented the absolute value of the surface height difference to 33.6 nm, which can be attributed to enhanced surface roughness, smaller fiber fragments, and flexible porous structure. Ultimately, it is suggested that the synergistic effect of SG and glutenin treatment on the improvement of the physicochemical and structural properties of WB powder may be linked to electrostatic interactions as well as hydrogen bond interactions. Overall, these findings can promote the technological development of modulating the physicochemical properties of WB to formulate novel whole grain flours.
研究领域:
食品科学
·
植物学
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产品关联
DOI
高影响因子
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
·
2025
·
IF 3.3
Yu Xiaohong,Zhang Ya,Wang Xianhao,Luo Yutong,Shao Shuai,Qiu Zhongyang
Organic acid pretreatment is one of the most promising methods to pretreat lignocellulose biomass due to its mild condition and weak corrosion to reactor. In this study, five different organic acids were used for wheat bran pretreatment, and the enzymatic hydrolysis yields of pretreated feedstocks were evaluated. Among these organic acids, oxalic acid pretreatment demonstrated the highest glucose yield of 87.93%. Subsequently, the optimization of oxalic acid pretreatment parameters was conducted including temperature, time, solid–liquid ratio, and acid usage. A high glucose yield of 96.92% was obtained at the optimal conditions: 0.8% of oxalic acid usage, 1:10 of solid–liquid ratio, and 130 ℃ for 15 min. FTIR, XRD and SEM revealed the mechanism of improved hydrolysis efficiency after oxalic acid pretreatment. Finally, the pretreated wheat bran was used for separate hydrolysis and ethanol fermentation (SHF) at 20% (w/w) solids loading. After 36 h, 23.87 g/L ethanol was produced by Saccharomyces cerevisiae DQ1 with all glucose consumed, with the yield of 0.12 g/g dry virgin wheat bran. This study provided a new insight into wheat bran pretreatment for biofuel production by a mild oxalic acid pretreatment method.
研究领域:
生物技术
·
可再生能源
·
微生物学
✓ 已验证
DOI
PubMed
高影响因子
Materials Chemistry Frontiers
·
2018
·Vol. 2
·期 10
·1784-1790
·
IF 0
Peng Lv,Sudong Yang,Peng-Cheng Ma
Developing novel materials for oil–water separation is highly desirable to solve the problems caused by oil spills. Herein, a wheat-bran-based hybrid material was prepared using a hydrothermal process, and the feasibility of utilizing the hybrid as an oil gelling agent (OGA) was studied. The results showed that the obtained material exhibited a mild hydrophobicity with a water contact angle above 130°, and an oleophilicity with high adsorption capacity for various oils and organic solvents. Furthermore, the material continuously adsorbed and gelled the oil floating on the water surface, forming an oil–OGA aggregate when in contact with an oil–water mixture. The aggregation-induced gelation observed in the material makes it possible to conveniently remove oil spills from the surface of water. The low cost and environmentally benign process distinguishes the developed material as a candidate that is suitable for the clean-up of oil spills.
研究领域:
材料科学
·
环境科学
·
生物技术
✓ 已验证
产品关联
DOI
RSC Advances
·
2025
·Vol. 15
·期 15
·12028-12041
·
IF 4.6
Wen Xuan Zheng,Tian Xiang Li,Yu-hui Xie,Ya Dong Lv,De Long Xie,Feng Wu
Multi-colored carbon dots (CDs) have attracted significant research interest due to their wide range of applications. However, the mechanisms underlying their luminescence and regulation still require further exploration. Herein, blue, green, and orange CDs with different quantum yields were successfully prepared, by changing the thermal degradation behavior of the waste wheat bran/o-phenylenediamine via adjusting the pH of the acidic hydrothermal conditions. The structure–photoluminescence property relationship of the prepared multi-colored CDs was thoroughly analyzed and discussed, aiming to provide new insights into the luminescence red-shift mechanisms of CDs. It was found that the larger sp2 domains, higher graphitization of carbon cores, increased graphitic nitrogen, and elevated levels of CO functional groups contribute to the CDs' lower band gap, resulting in a red-shift of the emission fluorescence. Among them, the elevated levels of CO functional groups are normally neglected for their contribution in the red-shift of CDs. Additionally, the prepared CDs were explored for their application in monitoring Fe3+ content in aquatic environments. A detection limit of 378.76 nM was obtained for the prepared CDs.
研究领域:
材料科学
·
环境科学
·
化学
✓ 已验证
DOI
PubMed
高影响因子
AQUACULTURE INTERNATIONAL
·
2022
·Vol. 30
·期 4
·1835-1853
·
IF 2.953
Wang Rui-Fang,Wang Yuan,Zhang Jun,Weng Mei-Qi,Liu Yu-Hui,Cheng Qiu-Yan,Song Min,Yang Yan-Ping,An Xiao-Ping,Qi Jing-Wei
Aquaculture research has focused on polysaccharides as they are among the most promising new-generation immunostimulant used to control aquatic diseases. The aim of this study was to investigate the immunomodulatory effects of fermented wheat bran polysaccharides (FWBPs) on juvenile common carp ( Cyprinus carpio ). Carps were fed different FWBP amounts (0%, 0.1%, 0.2%, and 0.4%) for 8 weeks and then their skin mucus and serum immune parameters, hepatopancreas antioxidant indicators, and immune-related gene expression in the intestines, kidneys, and spleen were measured. The skin mucus IgM levels significantly increased in 0.1% FWBP group, but decreased in 0.4% FWBP group. The skin mucus protease and the serum alkaline phosphatase activities increased significantly in the 0.2%, 0.1%, and 0.4% FWBP groups, respectively. The serum total Ig levels increased noticeably in the 0.1% and 0.2% FWBP groups. The highest and lowest serum lysozyme activities were observed in the 0.1% and 0.4% FWBP groups, respectively. The hepatopancreatic total superoxide dismutase activity was higher in the 0.1% FWBP group than in the control. The malondialdehyde levels decreased significantly in the 0.2% and 0.4% groups. The intestinal mRNA levels of the LZM - C and IL-10 genes were significantly higher in the 0.2% than in the 0.4% FWBP group; TNF-α was significantly upregulated in the 0.1% group. The gene expression in the kidneys did not differ significantly among the treatments, except for a significant increase in the IL-10 expression in the 0.1% treatment. Significantly elevated expression of LZM-C in 0.2% group and IL-10 in 0.1% group was observed in the spleens. TNF-α and IL-1β were significantly downregulated in the 0.4% group. These results suggest that FWBPs could be used as immunostimulant feed additives in common carp cultures.
研究领域:
水产养殖
·
免疫学
·
分子生物学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）