JOURNAL OF SEPARATION SCIENCE
·
2020
·Vol. 44
·期 5
·954-962
·
IF 2.878
Lu Kang,Ju Liu,Hongyang Zhang,Min Jiang,Yidian Jin,Min Zhang,Ping Hu
Creatinine and purines are gout-related metabolites commonly quantified by liquid chromatography coupled with ultraviolet and mass spectrometry. However, the high cost of liquid chromatography coupled with mass spectrometry hindered its extensive use in ordinary hospitals and clinical laboratories. Using the traditional liquid chromatography method, the full separation of these metabolites in complex biological samples is still not achieved. In this study, an improved ultra-high-performance liquid chromatography with ultraviolet spectroscopy method was reported for quantitative determination of five gout-related metabolites (i.e., creatinine, uric acid, hypoxanthine, xanthine, and inosine) in human serum within 10 min. A UHPLC system equipped with a hydrophilic C18 column was used to improve separation, shorten analysis time, and increase analysis throughput. The performance of the method was validated by evaluating linearity (squared correlation coefficient > 0.9991), recovery (92.8–100.0%, with relative standard deviation < 4.7%), accuracy (relative errors < 14.6%), precision (0.2-4.1% for intraday and 2.1-7.3% for interday) and stability (-14.1 to 8.3% in autosampler for 12 h and -13.3 to 2.2% for freeze-thaw cycles). This method was successfully applied to quantify gout-related metabolites in serum samples of healthy controls and gout patients, which was expected to be used in the clinical investigation of gout at different stages.
研究领域:
医学检验
·
代谢组学
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DOI
PubMed
高影响因子
Soft Matter
·
2024
·Vol. 20
·期 22
·4422-4433
·
IF 2.9
Xiubin Hou,Yingxia Wang,Xinbing Song,Juan Gao,Yurong Ma
Biogenic purine crystals can function in vision as light scatters, mirrors, and multilayer reflectors and produce structural colors or depolarization for camouflage. Xanthine crystals form irregular multifocal mirrors in the median ocellus of Archaeognatha. It is important to broaden the study of crystallization strategies to obtain organic crystals with purine rings in the laboratory. In this work, a facile one-step synthesis route to fabricate bio-inspired xanthine crystals is reported for the first time. The obtained rhomboidal xanthine nanoplates have similar morphology and size to biogenic xanthine crystals. Their length and thickness are about 2–4 μm and 50 nm, respectively. Lattice parameters, crystal structure, formation mechanism and optical properties of synthetic single-crystalline xanthine nanoplates were investigated in detail in this work. The obtained xanthine nanoplate crystals are proposed to be anhydrous xanthine with monoclinic symmetry, and the xanthine nanoplates mainly expose the (100) plane. It is proposed that the anhydrous xanthine nanoplates are formed via an amorphous xanthine intermediate precursor. The synthetic anhydrous xanthine nanoplates exhibit excellent optical properties, including high diffuse reflectivity, strong depolarization and pearlescent luster. This work provides a new design to synthesize bio-inspired organic molecular crystals with excellent optical properties.
研究领域:
晶体学
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生物材料
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光学
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DOI
PubMed
高影响因子
ELECTROANALYSIS
·
2022
·Vol. 35
·期 4
·e202200403
·
IF 3.077
Fuxin Liu,Tianyi Shang,Lingling Han,Nan He,Xiuhui Liu
Xanthine is a significant biomolecule and its concentration level in urine and blood plasma is an indicator of specified pathological states. Here, a new sensing platform was designed, which showed excellent analytical performance for xanthine. Importantly, it is the first time to investigate the kinetics of xanthine metabolic reaction by electrochemical method. The results demonstrated that the conversion of xanthine to uric acid completely conformed to the Michaelis-Menten kinetics. Furthermore, we also studied the inhibitory effect of febuxostat on xanthine oxidase activity detailed. As expected, the work may offer potential value for researchers in the treatment of hyperuricemia and gout.
研究领域:
生物化学
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医学
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药物研究
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DOI
高影响因子
Journal of Central South University
·
2014
·Vol. 21
·期 3
·870-876
·
IF 0.464
Dou Zhi-yu,Cui Li-li,He Xing-quan
The novel covalently modified glassy carbon electrode with poly(xylitol) was prepared using an electropolymerization technique for the simultaneous determination of uric acid (UA), xanthine (XA) and hypoxanthine (HX). This new electrode presents an excellent electrocatalytic activity towards the oxidation of UA, XA and HX by cyclic voltammetry (CV) method. The oxidation peaks of the three compounds were well defined and had enhanced the peak currents. The separation potentials of the oxidation peak potentials for UA-XA and XA-HX were 380 and 370 mV in CV, respectively. Using differential pulse voltammetry (DPV) method, the calibration curves in the ranges of 5–55, 1.3–75.3 and 4–59 μmol/L were obtained for HX, XA and UA, respectively. The lowest detection limits (S/N=3) were 4.5, 0.75 and 3.75 μmol/L for HX, XA and UA, respectively. The practical application of the modified electrode was demonstrated by the determination of UA, XA, HX in human urine samples.
研究领域:
分析化学
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医学诊断
·
电化学
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DOI
高影响因子
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
·
2017
·
IF 3.012
Yangping Wen,Juan Chang,Lanjiao Xu,Xiaoning Liao,Ling Bai,Yingdong Lan,Mingfang Li
Abnormal level of purines , especially uric acid (UA), xanthine (XA) and hypoxanthine (HX) in organisms is an important marker for early diagnosis of animal (human) disease. In this paper, an simultaneous analysis of UA, XA and HX in biological samples was successfully realized using the fabricated voltammetric sensor based on the nanocomposite of both nano-rod clay mineral palygorskite (Pal) and two-dimensional nano-layered nitrogen doped graphene (NG). A water-soluble carboxymethyl cellulose (CMC) salt was simultaneously decorated both Pal and NG to improve the water dispersibility, and their nanocomposite was characterized. Under optimized conditions, The as-prepared nanocomposite electrode (CMC-Pal-NG/GCE) could realize their simultaneous voltammetric analysis in linear ranges from 0.5 to 60 μM for UA, 0.2 to 20 μM for XA, and 0.6 to 55 μM for HX. The limit of detection of three purines (UA,XA and HX) is calculated to be 0.1 for UA, 0.05 for XA and 0.4 μM for HX, and their possible detailed mechanisms for electro-catalytic oxidation and chemical reaction was discussed, which was successfully empolyed for simultaneous voltammetric analysis of three purines in real samples with good reproducibility, repeatability and stability. Satisfactory results indicated that the obtained nanocomposite will provide a promising candidate for the simultaneous electrochemical determination of UA, XA and HX in analysis of practical samples.
研究领域:
分析化学
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生物医学工程
·
材料科学
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产品关联
DOI
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2019
·
IF 9.518
Ting Xue,Yingying Sheng,Jingkun Xu,Yingying Li,Xinyu Lu,Yifu Zhu,Xuemin Duan,Yangping Wen
The environmental stability, water-processibility and life-span of black phosphorene (BP) severely limit the application of its electronic devices in aqueous system containing oxygen. We reported the controllable preparation of in-situ reduction and deposition of silver nanoparticles on the BP surface and its amino-functionalized multi-walled carbon nanotubes (NH 2 -MWCNT) nanocomposite . With the addition of both NH 2 -MWCNT and Ag + , the BP-based nanocomposite was prepared by ultrasonic-assisted liquid-phase exfoliation and was dispersed in carboxymethyl cellulose sodium (CMC) aqueous solution. The morphology, microstructure, and electrochemical properties of the nanohybrid were characterized. NH 2 -MWCNT-BP-AgNPs showed high environmental stability, good water-processibility, satisfactory life-spans, superior electrocatalytic capacity with enzyme-like kinetic characteristics. The nanohybrid was applied as electrochemical sensors for single/simultaneous analysis of uric acid (UA), xanthine (XT) and hypoxanthine (HX). Excellent voltammetric responses for simultaneous determination in linear ranges of 0.1–800 μM with a limit of detection (LOD) of 0.052 μM for UA, 0.5–680 μM with a LOD of 0.021 μM for XT, and 0.7–320 μM with a LOD of 0.025 μM for HX under optimal conditions. Besides, the developed nanozyme sensor was employed for simultaneous voltammetric analysis of UA, XT and HX in real samples with acceptable recoveries. This work will provide theoretical guidance and experimental support for the preparation and application of two-dimensional materials, nanozymes and sensing devices.
研究领域:
材料科学
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生物医学工程
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分析化学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF MATERIALS SCIENCE
·
2023
·Vol. 58
·期 17
·7441-7455
·
IF 4.5
Wang Yijie,Li Qiulan,Xia Qinghai,Yang Yaling,Yang Dezhi
Sensitive and effective detection of xanthine oxidase (XOD) is essential for assessing human health. Herein, a dual-enzyme-based cascaded reaction system was developed for xanthine and xanthine oxidase assay based on the peroxidase (POD)-like catalytic activity of Fe 3 O 4 @MWCNTs/Hemin nanocomposite. The XOD could catalyze xanthine to produce H 2 O 2 , which is subsequently converted by Fe 3 O 4 @MWCNTs/Hemin into hydroxyl radicals (·OH) to oxidize 3,3′,5,5′-tetramethylbenzidine (TMB) to produce a light blue. Notably, it is demonstrated that the modification of MWCNTs and Hemin could significantly enhance the POD-like activity from 9.78 × 10 −8 M s −1 ( V max value of Fe 3 O 4 ) to 23.34 10 −8 M s −1 ( V max value of Fe 3 O 4 @MWCNTs/Hemin), which makes the detection of xanthine oxidase more sensitive. By using the smartphone to capture the colorimetric signal of oxidized TMB (oxTMB), the assay can detect xanthine and XOD as low as 0.250 µM and 0.216 mU mL −1 , respectively. The reliability of the method in human serum suggested satisfactory spiked recoveries in the range of 92.350–105%. This work provided a new insight for Fe 3 O 4 -based nanozyme design and monitoring XOD level in blood. Graphical Abstract
研究领域:
生物化学
·
纳米技术
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分析化学
·
医学诊断
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产品关联
DOI
高影响因子
TALANTA
·
2013
·
IF 3.498
Min Liu,Shanshan Chen,Xianzheng Zhao,Yuhang Ye,Juan Li,Qinshu Zhu,Bo Zhao,Wenbo Zhao,Xiaohua Huang,Jian Shen
A novel hypoxanthine biosensor fabricated by immobilizing the xanthine oxidase (XOD) onto the phosphonic acid-functionalized silica (SiO 2 -P) film on the surface of glassy carbon electrode (GCE) was designed and constructed in this work. A biomimetic platform was designed with the phosphonic acid-functionalized silica nanoparticles (SiO 2 -P NPs) synthesized by the method of reverse microemulsion and electrostatic binding. In such a platform, XOD was selected as model protein to fabricate hypoxanthine biosensor based on SiO 2 -P NPs. The nanocomposite was characterized with transmission electron microscopy (TEM), energy dispersive X-ray spectrometer (EDS) and electrochemical impedance spectroscopy (EIS). Based on the advantageous functions of SiO 2 -P NPs, the entrapped XOD could preserve its bioactivity and exhibited an excellent electrochemical behavior with a formal potential of −0.37 V in phosphate buffer solution (PBS, pH=7). Response studies to hypoxanthine were carried out using current–time response curve. The biosensor exhibited a wide linear response ranging from 1.00×10 −6 to 2.61×10 −4 M. The detection limit of 2.33×10 −7 M at a signal-to-noise ratio of 3 was lower than that most reported previously. In addition, the electrode modified with XOD/(SiO 2 -P NPs) film also had a strong anti-interference ability in the presence of uric acid (UA) and ascorbic acid (AA). The assay results of hypoxanthine in fish samples were in a good agreement with the reference values.
研究领域:
分析化学
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生物医学工程
·
纳米技术
✓ 已验证
DOI
高影响因子
Food Analytical Methods
·
2019
·Vol. 12
·期 8
·1715-1724
·
IF 2.413
Wang Zhipan,Ma Baokai,Shen Cai,Lai Oi-Ming,Tan Chin-Ping,Cheong Ling-Zhi
ATP degradation is an important biochemical change during decomposition of seafood. Hypoxanthine and xanthine which are formed during ATP degradation process can be used for evaluation of chilled seafood freshness. Present study successfully immobilized xanthine oxidase (XOD) onto a type of biocompatible copper-based metal organic framework nanofibers (Cu-MOF) film and used it for fabrication of a hypoxanthine and xanthine electrochemical biosensor. Cu-MOF can efficiently entrap XOD, provide a suitable atmosphere for XOD biocatalysis, and ensure good electron transfer between enzyme and electrode surface. The as-prepared XOD-electrochemical biosensor demonstrated high sensitivity for both hypoxanthine and xanthine with a wide linear range (0.01 to 10 μM) and low limit of detection. During the 3-week storage stability test at 4 °C, the fabricated biosensor demonstrated good reusability (up to 100 times) and excellent storage stability for hypoxanthine and xanthine. When applied for detection of hypoxanthine and xanthine in chilled squid and large yellow croaker, the XOD-electrochemical biosensors demonstrated good recovery rates.
研究领域:
食品科学
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生物化学
·
传感技术
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DOI
高影响因子
MICROCHIMICA ACTA
·
2018
·Vol. 186
·期 1
·1-10
·
IF 5.705
Zhang Li,Li Shaobin,Xin Jianjiao,Ma Huiyuan,Pang Haijun,Tan Lichao,Wang Xinming
A Cr-based metal-organic framework MIL-101(Cr) was used to load platinum nanoparticles (PtNPs) that were placed on a glassy carbon electrode (GCE). The modified GCE was used as a non-enzymatic xanthine sensor. Compared to bare GCE, it requires a strongly decreased working potential and an increased signal current for xanthine oxidation. This is due to the crystalline ordered structure and large specific surface of the MIL-101(Cr), and to the high conductivity of the Pt NPs. Differential pulse voltammetry (DPV) shows the sensor to have a wide linear range (0.5 – 162 μM), a low detection limit (0.42 μM), and high selectivity. It was applied to the simultaneous determination of dopamine, uric acid, xanthine and hypoxanthine at working potentials of 0.13, 0.28, 0.68 and 1.05 V, respectively (vs. Ag/AgCl) and to quantify xanthine in spiked serum samples.
This is the first report of non-enzymatic xanthine electrochemical sensor based on metal-organic framework loaded with nanoparticles.
研究领域:
生物医学工程
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Zhuoran Li,Limin Cao,Jianxin Sui,Lei Wang,Hong Lin,Kaiqiang Wang
The timely detection of freshness changes of aquatic products is crucial. In this study, we have developed a reliable, cost-effective, and user-friendly method for rapidly detecting hypoxanthine using a xanthine oxidase (XOD)/nanozyme enzymatic cascade system. The nanozyme, derived from the Fe 7 /Ni 3 metal–organic framework (Fe 7 Ni 3 MOF), exhibited good peroxidase-mimetic activity and stability. Our proposed XOD/Fe 7 Ni 3 MOF enzymatic cascade system demonstrated a linear response to hypoxanthine in the range of 3–70 μM, with a low detection limit of 1.39 μM. We also analyzed hypoxanthine in actual aquatic products, achieving spiked recoveries ranging from 90.04 % to 107.37 %. The correlation coefficient between our developed colorimetric method and the HPLC method was 0.98. Importantly, our proposed method holds several advantages over alternative techniques, particularly in terms of cost-effectiveness, precision, and speed. Consequently, this methodology shows great promise for the early detection of freshness changes in aquatic samples.
研究领域:
分析化学
·
食品科学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2021
·
IF 4.098
Zehuai Mou,Zhijin Gao,Yaoping Hu
Sensitive determination of hypoxanthine (HX), an indicator reflecting the degradation of meat and fish, is significantly important in monitoring food freshness. Herein, we design a novel sensor consisting of orange emissive carbon dots (O-CDs), nitrotetrazolium blue chloride (NTBC), and xanthine oxidase (XOD) for fluorescence turn-off detection of HX. O-CDs, possessing a high fluorescence quantum yield of 37%, are synthesized by hydrothermal treatment of 2,3-diaminopyridine in sulfuric acid. NTBC can react with HX/XOD-generated H 2 O 2 and O 2 – to yield a violet-colored formazan, which remarkably quenches the orange fluorescence of O-CDs through inner filter effect. There is a linearity between the quenching efficiency and HX concentration in the range of 2–250 μM, and the limit of detection is 0.61 μM, lower than those of most reported HX sensors. In addition, the proposed method exhibits excellent selectivity, and can be applied to quantify HX in fish samples with satisfactory results.
研究领域:
分析化学
·
食品科学
·
生物技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）