INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
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2020
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IF 4.187
Yanbo Wang,Feifei Wang,Xiaoshuang Zhang,Congnan Cen,Linglin Fu
Shewanella baltica is the specific spoilage microorganism of Pseudosciaena crocea during low-temperature storage. Exploring the correlation between cold adaptability and spoilage potential may provide a new perspective for prolonging shelf life of aquatic products. In the present study, we investigated the synthesis pathway of unsaturated fatty acid (UFA) responsible for regulating cold adaptability in Shewanella baltica and its effect on spoilage potential. FabR and FadR, as key regulators of membrane unsaturated fatty acids synthesis pathway, were identified in S. baltica . FabR was significantly down-regulated at 4 °C compared to at 30 °C, yet FadR displayed the opposite results. By overexpressing fabR and fadR genes at 4 °C, we found that FabR and FadR had negative and positive effects on UFA content and membrane fluidity as well as spoilage potential, respectively. These data indicated that FabR and FadR functioned collectively to increase the membrane fluidity for better cold adaptability at low temperature, resulting in the maintenance of spoilage potential of S. baltica .
研究领域:
微生物学
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食品科学
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分子生物学
✓ 已验证
DOI
PubMed
高影响因子
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
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2019
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IF 2.697
Siwen Kuang,Qijun Le,Jiabao Hu,Yajun Wang,Na Yu,Xiaohuan Cao,Man Zhang,Yibo Sun,Weiwei Gu,Yang Yang,Youyi Zhang,Yaya Li,Hanwei Liu,Xiaojun Yan
p-Nitrophenol (PNP) is one type of environmental pollutant, which is difficult to degrade and soluble in water. To investigate the effects of PNP on economically important marine fish species, we subjected Larimichthys crocea juvenile to five different concentrations of PNP for 96 h, and the semi-lethal concentration (LC50) was 6.218 mg/L. Then we collected the liver, kidney, and gill tissues to determine enzyme activity and gene expression levels , and analyzed histological changes. In histological analysis, the gills showed curling of lamella, epithelial lifting and hyperplasia ; the parenchymal structure of hepatocytes was significantly damaged, with severe vacuolation and loss of original structure. The renal cells were damaged too, with congestion and renal tubular necrosis . Catalase and superoxide dismutase both showed an up- and down-tendency with the rise of concentration in the three tissues, and GSH-px had similar trend in the kidney, which decreased at 8 mg/L in the liver but showed no significant differences in the gills. Malondialdehyde of three tissues was increased with an increase in PNP concentration. The expression of four detoxification ( cyp450 , gst , gpx, hsp70 ) and one immune-related ( mhc II ) genes was induced at low PNP concentrations but inhibited at high PNP concentrations in the kidney. In liver, cyp450, hsp70 and mhc II showed similar trend but gst and gpx didn't increase at low PNP concentrations. Our results indicate that the fish possesses the ability to detoxify PNP; however, at high concentrations, PNP still causes serious damage to them. Our data not only help in understanding the ability of L. crocea to detoxify PNP but also should serve as a basis for the study of toxic effects of nitrobenzenes on marine fish.
研究领域:
海洋生物学
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环境毒理学
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分子生物学
✓ 已验证
DOI
PubMed
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
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2022
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IF 6.056
Qibin Li,Quanyi Chen,Yueying Wu,Zhibao Chen,Ying Liu,Zhijia Fang,Qi Deng
The bacteriocin CAMT6 is a novel bacteriocin produced by Enterococcus durans YQ-6 isolated from Larimichthys polyactis in the South China Sea. In this study, the bacteriocin CAMT6 was purified by extraction using ethyl acetate, Sephadex LH-20 column chromatography, cation exchange chromatography and reversed-phase high-performance liquid chromatography, in sequence. The Liquid Chromatograph Triple Quadrupole Mass Spectrometer (LC-MS/MS) analysis revealed that the relative molecular mass and amino acid sequence of the bacteriocin CAMT6 were 1254.63 Da and N-GAVHDVKDVLDS, respectively. Combined with the circular dichroism (CD) spectroscopy results, CAMT6 was determined to be a novel Class IId bacteriocin. CAMT6 exhibited broad-spectrum antibacterial activity, inhibiting both gram-negative and gram-positive bacteria, even fungi. CAMT6 exhibited strong temperature stability, was stable over pH 2–7, and was inactivated by proteases. CAMT6 also significantly removed L. monocytogenes biofilms and inhibited L. monocytogenes by disrupting the permeability of the cell membrane. Furthermore, CAMT6 can effectively inhibit the growth of L. monocytogenes in chicken breast. These results suggest that the bacteriocin CAMT6 could serve as a potential biological preservative for food.
研究领域:
微生物学
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食品科学
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生物化学
✓ 已验证
产品关联
DOI
高影响因子
FOOD RESEARCH INTERNATIONAL
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2024
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IF 7
Shengliang Zhang,Hongyun Lu,Hanghang Lou,Ying Shi,Donghong Liu,Qihe Chen
The large-scale production of cultured meat has been restricted by high cost of serum, batch difference and unclear composition, thus it is important to develop serum-free media for cell growth. Here we obtained a serum-free medium (SFM), which was suitable for ex vivo culturing of myoblasts from larimichthys crocea . It offered simple composition and high effectiveness that supporting myoblast attachment, expansion and differentiation. Our findings revealed that the optimal SFM consisted of DMEM/F12 base medium, 5 mg/mL bovine serum albumin (BSA), 20 mmol HEPES, 1 % penicillin/streptomycin mixture (PS) and the exogenous additive hydrocortisone. The living cells of SFM could occupy about 65 % of the serum-containing medium (SM). Additionally, differentiation assays showed that myoblasts cultured without serum still differentiated into more muscle tubes, with a higher fusion index than those cultured with serum. Finally, transcriptome sequencing was conducted to demonstrate that hydrocortisone might act as a ligand binding to G protein-coupled receptors, triggering the downstream PI3K/AKT signaling pathway and leading to cell proliferation. Our findings will provide a basis for large-scale cell expansion and cell culture meat production in the future.
研究领域:
细胞生物学
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组织工程
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生物技术
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食品科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
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2022
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IF 9.231
Okon Johnson Esua,Da-Wen Sun,Jun-Hu Cheng,Huifen Wang,Chen Chen
Hybridising plasma functionalized water and ultrasound pretreatment for the enzymatic hydrolysis (HPUEH) of Larimichthys polyactis was evaluated by adopting Plackett-Burman design for parametric screening of six key variables, and Box-Behnken design for optimizing three most significant variables including plasma generating voltage (P V ), ultrasound treatment time (U T ), and enzyme concentration (E C ). The models developed for predicting the degree of hydrolysis (DoH), protein recovery (PVY), and soluble protein content (SPC) were sufficiently fitted to the experimental data (R 2 ≥ 0.966) with non-significant lack of fit and used for determining the optimum conditions as P V of 70 V, U T of 15 min, and E C of 1.787%, with predictive values of 27.74%, 85.62%, and 3.28 mg/mL for DoH, PVY, and SPC, respectively. HPUEH presented hydrolysates with smaller peptide sizes and molecular weights, enhanced DoH, PVY, SPC, amino acids and antioxidant activity, but reduced emulsifying and foaming properties when compared with conventional enzymatic hydrolysis.
研究领域:
食品科学
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生物技术
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化学工程
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生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
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