MICROCHIMICA ACTA
·
2022
·Vol. 190
·期 1
·1-10
·
IF 6.408
Zhu Jing,Xu Wenxing,Yang Ye,Kong Rongmei,Wang Junmei
A nanozyme sensor array based on the ssDNA-distensible C3N4 nanosheet sensor elements for discriminating multiple mycotoxins commonly existing in contaminated cereals has been explored. The sensor array exploited (a) three DNA nonspecific sequences (A40, T40, C40) absorbed on the C3N4 nanosheets as sensor elements catalyzing the oxidation of TMB; (b) the presence of five mycotoxins affected the catalytic activity of three nanozymes with various degrees. The parameter (A 0 -A) was employed as the signal output to obtain the response patterns for different mycotoxins with the same concentration where A 0 and A were the absorption peak values at 650 nm of oxTMB in the absence and presence of target mycotoxins, respectively. After the raw data was subjected to principal component analysis, 3D canonical score plots were obtained. The sensor array was capable of separating five mycotoxins from each other with 100% accuracy even if the concentration of the mycotoxins was as low as 1 nM. Moreover, the array performed well in discriminating the mycotoxin mixtures with different ratios. Importantly, the practicality of this sensor array was demonstrated by discriminating the five mycotoxins spiking in corn-free samples in 3D canonical score plots, validating that the sensor array can act as a flexible detection tool for food safety. Graphical abstract A nanozyme sensor array was developed based on the ssDNA-distensible C3N4 NSs sensor elements for discriminating muitiple mycotoxins.
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TALANTA
·
2023
·
IF 6.1
Guo Ge,Tianlin Wang,Zihou Liu,Xiaomeng Liu,Tiange Li,Yuntang Chen,Jialin Fan,Erkigul Bukye,Xianqing Huang,Lianjun Song
Realizing the simultaneous speedy detection of multiple mycotoxins in contaminated food and feed is of great practical importance in the domain of food manufacturing and security. Herein, a fluorescent aptamer sensor based on self-assembled DNA double-crossover was developed and used for effective simultaneous quantitative detection of aflatoxins M 1 and B 1 by fluorescence resonance energy transfer (FRET). Fluorescent dye-modified aflatoxin M 1 and B 1 aptamers are selected as recognition elements and signal probes, and DNA double crosses are consistently locked by the aflatoxin aptamers, which results in a “turn-off” of the fluorescent signal. In the presence of AFM 1 and AFB 1 , the aptamer sequences are more inclined to form Apt-AFM 1 and Apt-AFB 1 complexes, and the fluorescent probes are released from the DNA double-crossing platform, leading to an enhanced fluorescent signal (Cy3: 568 nm; Cy5: 660 nm). Under the optimal conditions, the signal response of the constructed fluorescent aptamer sensor showed good linearity with the logarithm of AFM 1 and AFB 1 concentrations, with detection limits of 6.24 pg/mL and 9.0 pg/mL, and a wide linear range of 0.01–200 ng/mL and 0.01–150 ng/mL, respectively. In addition, the effect of potential interfering substances in real samples was analyzed, and the aptasensor presented a good interference immunity. Moreover, by modifying and designing aptamer probes, the sensor can be applied to high-throughput simultaneous screening of other analytes , providing a new approach for the development of fluorescent aptamer sensors.
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2022
·
IF 5.895
Meng Chen,Ming Li,Wenxi Zhang,Hua Bai,Qiang Ma
Green analytical chemistry (GAC) represents a rapidly growing research field that aims at developing novel analytical approaches with minimal consumption of hazardous reagents and solvents. The current study reports on a GAC methodology exploiting the unique physicochemical properties of natural deep eutectic solvents (NADESs), a supposedly environmentally friendly class of solvents. Based on a temperature-mediated strategy, the NADESs were manipulated to undergo multiple phase transitions for favorable functionality and performance. As proof-of-concept demonstrations, both hydrophobic and hydrophilic NADESs were prepared for the extraction and analysis of eight phthalate esters in aqueous samples (food simulants) and three aflatoxins in oily samples (edible oils), respectively. NADES-based dispersive liquid–liquid microextraction (DLLME) was employed to achieve high-efficiency sample pretreatment. Afterward, the NADESs were transformed from liquids into solids by tuning the peripheral temperature for a convenient phase separation from the sample matrices. The solidified NADES extracts were melted and vaporized at elevated temperatures by transmission-mode direct analysis in real time (DART) for further quadrupole-Orbitrap high-resolution mass spectrometry (Q-Orbitrap HRMS) analysis. The developed protocol was validated, achieving good repeatability with relative standard deviations (RSDs) of less than 9% and satisfactory sensitivity with limits of detection (LODs) and quantitation (LOQs) ranging from 0.1 to 0.8 and 0.2 to 2.0 μg/kg, respectively. The greenness of the analytical methodology was assessed with the calculated scores of 0.66 and 0.57 for the hydrophobic and hydrophilic NADES-based protocols, respectively. The method was applied to marketed samples, highlighting the great potential for green chemical analysis.
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MICROCHEMICAL JOURNAL
·
2023
·
IF 4.8
Peifang Chen,Caiyun Jiang,Zhouping Wang,Hong-zhen Lian,Xiaoyuan Ma
As a virulent metabolite, aflatoxin B 1 (AFB 1 ) presented in various cereal grain is tightly implicated in severe human diseases. In this study, 3D plasmonic nanohybirds of Raman molecule 4-mercaptobenzoic acid (4-MBA)-embedded and AFB 1 aptamer-modified bimetallic nanostars as SERS probes bound to magnetic nanoflowers were fabricated and demonstrated as a high-performance SERS-active aptasensor to quantitatively analyze AFB 1 . Bimetallic Au@Ag SERS plasmonic nanoprobes with enhanced properties were capable of enhancing discriminative Raman peaks of 4-MBA. Then, the integration of iron tetroxide nanoparticles (Fe 3 O 4 NPs) and molybdenum disulfide nanosheets (MoS 2 NShs) with huge specific surface area constituted stable 3D Fe 3 O 4 @MoS 2 plasmonic nanoflowers, facilitating the bind of numerous aptamer-based SERS probes via the non-covalent interaction between MoS 2 NShs and aptamer, which were ideal candidates for SERS-active substrates. Additionally, Fe 3 O 4 NPs as magnetic core endowed 3D nanocomposites with specific magnetic separation characteristic that caused the collected SERS hotspots to exhibit superior signal response, and further strengthening the sensitivity in a complex food matrix. Aptamer-target AFB 1 specific recognition triggered linearly diminished 4-MBA signal intensity (I 4-MBA ) on the substrate to achieve a low detection limit of 58.9 pg/mL. Furthermore, the sensor has the potential to be a promising monitoring tool for other trace contaminants.
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FOOD CHEMISTRY
·
2023
·
IF 8.8
Ding Yang,Yuanyuan Hui,Yingying Liu,Weizhe Wang,Chao He,Aiqing Zhao,Lusha Wei,Bini Wang
Aflatoxin M 1 (AFM 1 ) is a highly carcinogenic and toxic compound that is commonly found in dairy foods such as milk. We developed an electrochemical MIP (molecularly imprinted polymer) and aptamer (Apt) based aptasensor (Apt-Au@PEIM/AFM 1 /MIP–Apt/AuNPs)/GCE) with dual-recognition signal amplification to detect AFM 1 in milk. The MIP–Apt/AuNP/GCE sensor was prepared by synthesizing an MIP film through the electropolymerization of resorcinol on gold nanoparticles-modified electrodes. The dropwise addition of our cApt-Au@PEIM signal amplification probe could enhance the current signal response and improve the sensitivity of the sensor to detect AFM 1 . The AFM 1 present in milk could prevent the probe from binding to the sensor, thereby drastically reducing the current signal. Under optimal experimental conditions, the sensor exhibits a linear range of 0.01–200 nM and a limit of detection of 0.07 nM (S/N = 3). The developed dual-recognition aptasensor would provide a novel and rapid method for specific detection of AFM 1 in milk.
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FOOD CHEMISTRY
·
2023
·
IF 8.8
Wenting Li,Yongqiang Shi,Xinai Zhang,Xuetao Hu,Xiaowei Huang,Nini Liang,Tingting Shen,Xiaobo Zou,Jiyong Shi
Herein, a bifunctional electrochemical biosensor based on the DNA tetrahedral scaffolds (TDNs) was proposed, OTA@TDNs and AFB1@TDNs were adopted for electrochemical signal output in response to OTA and AFB1 concentration, simultaneously. In order to increase the conductivity of the biosensor, highly porous gold (HPG) was loaded on electrode surface by pulse electrodeposition. Under optimal conditions, the P Fc displayed a linear range with AFB1 concentration between 0.05 ∼ 360 ng·mL −1 with the LOD of 3.5 pg·mL −1 . And the P MB selective and sensitive responses to OTA are achieved with a linear range of 0.05 ∼ 420 ng·mL −1 and a LOD of 2.4 pg·mL −1 . This biosensor has high sensitivity, selectivity and stability for OTA and AFB1 detection in peanut samples. The approach streamlines the experimental procedure, leading to significantly improve the detection efficiency of mycotoxins. Collectively, this method suggest a novel approach for the detection and monitoring of OTA and AFB1 in food sample.
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ANALYTICAL CHEMISTRY
·
2019
·
IF 6.35
Zhiwei Qie,Qiqi Liu,Wenliang Yan,Zichen Gao,Wu Meng,Rui Xiao,Shengqi Wang
A universal and ultrasensitive immunochromatographic assay (ICA) was established using antigen as a bifunctional element and antialbumin antibody in a test line. Preincubation was introduced for competitive recognition. After optimization, the linear detection of aflatoxin M1 (AFM1) with quantum dot bead (QB)-based ICA (QB-ICA) sensor ranged from 10 to 52 pg mL–1, with a 50% inhibitory concentration (IC50) of 23 pg mL–1, which was nearly 49.6-fold lower than those of ICA on a traditional structure with traditional pretreatment (IC50 = 1.10 ng mL–1) and 10-fold lower than those of ICA on a traditional structure with acid aid pretreatment (IC50 = 0.25 ng mL–1). The limit of detection (LOD) for AFM1 was 16 pg mL–1 in milk, which was approximately 16.3-fold times higher than those of ICA on a traditional structure with traditional pretreatment and 6.3-fold higher than those of ICA on a traditional structure with acid aid pretreatment. The LOD improved by 20-fold by using the proposed structure compared to that of conventional enzyme-linked immunosorbent assay (ELISA) for AFM1-spiked milk samples (IC10 = 0.12 ng mL–1). The performance and practicability of the established QB-ICA sensor were validated with a commercial ELISA kit. To evaluate universality, we successfully detected chloramphenicol, with IC50 of 0.42 ng mL–1. Given its high sensitivity and universality, the proposed QB-ICA can be used as an alternative for rapid, sensitive, and universal quantitative detection of all small-molecule analytes.
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JOURNAL OF FOOD COMPOSITION AND ANALYSIS
·
2025
·
IF 4.6
Meijun Lu,Tianlin Wang,Tiange Li,Yan Ma,Xianqing Huang,Libin Wan,Fayun Wang,Lianjun Song
A ratiometric aptasensor for deoxynivalenol (DON) detection was developed based on FRET between graphene oxide (GO) and carbon dots. Blue carbon dots, functionalized with aptamers, act as energy donors, while GO functions as the energy acceptor. In the absence of DON, the addition of GO quenches the fluorescence of the blue carbon dots, while the fluorescence of red carbon dots, used as internal reference signals, remains unchanged. When DON is introduced, the aptamer selectively binds to the target, increasing the distance between the donor and acceptor, disrupting FRET, and gradually restoring the blue fluorescence. The sensor demonstrated a strong linear relationship within the concentration range of 0.05–200 ng/mL of DON, with a detection limit of 14.7 pg/mL. The superior analytical performance and high sensitivity indicate the potential of the sensor for DON detection in real sample matrices.
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JOURNAL OF FOOD COMPOSITION AND ANALYSIS
·
2025
·
IF 4.6
Tianlin Wang,Meijun Lu,Yanbing Shen,Qinqing Han,Songming Wang,Tongyao Li,Tiange Li,Xianqing Huang,Libin Wan,Lianjun Song
Excessive levels of penicillin (PAT) in fruit juice pose significant risks to food safety and human health. In this study, a ratiometric fluorescence sensor with on-site visualization detection capability had been developed for PAT detection based on Förster resonance energy transfer (FRET) in this study. This strategy utilized MIL-101(Cr) with a wide absorption peak as the energy acceptor, while nitrogen/sulfur-doped graphene quantum dots (N,S-GQDs) conjugated with PAT aptamer with stable blue fluorescence serves as the energy donor, triggering the FRET effect and quenching the blue fluorescence. When exposed to PAT, the aptamer preferentially binded to PAT, separating the donor and acceptor, thereby interrupting the FRET effect and recovering the blue fluorescence. Assisted by the red internal reference fluorescence of gold clusters (AuNCs), the fluorescence changes were observed from red to blue with the concentration range of 0.01–300 ng/mL of PAT and a low limit of detection at 3.29 pg/mL. More importantly, a portable detection device integrated with smartphone was designed for on-site visual PAT detection with satisfactory results. This strategy could provide new opportunities for on-site qualitative and quantitative detection of PAT in actual foods.
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TALANTA
·
2025
·
IF 6.1
Ke Wang,Yingqi Wang,Jiayao Wang,Yiwei Liu,Xiaopeng Hu
In this work, we developed a ratiometric fluorescence/colorimetric dual-mode sensor based on the synergistic recognition of aptamer and molecularly imprinted polymers (MIPs) for ultrasensitive detection of AFB 1 . Polydopamine/carbon dots-functionalized Fe 3 O 4 (MIP/CDs/Fe 3 O 4 ) serves as both a reference probe and a recognition element. The aptamer-modified Rhodamine 6G-embedded ZIF-8 (Apt-Rh6G@ZIF-8) acts as both a fluorescence probe and a colorimetric probe, enabling simultaneous dual-mode detection. In the presence of AFB1, AFB1 is captured by the MIP/CDs/Fe 3 O 4 via molecular recognition, forming sandwich-structured particles designated as MIP/CDs/Fe 3 O 4 ∼AFB1∼Apt-Rh6G@ZIF-8. These complexes are magnetically separated, allowing ratiometric fluorescence analysis based on the CDs/Rh6G. Simultaneously, the unbound Apt-Rh6G@ZIF-8 remaining in the suspension exhibits visible color changes, achieving colorimetric analysis. The sensor demonstrates a linear range of 0.01–15 ng mL −1 (fluorescence) and 0.1–15 ng mL −1 (colorimetric), with detection limits as low as 8.5 pg mL −1 (fluorescence) and 15 pg mL −1 (colorimetric), respectively. Notably, the platform achieved accurate AFB1 quantitation in crude edible oil samples without pretreatment, demonstrating its high practicality for real-world applications.
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LWT-FOOD SCIENCE AND TECHNOLOGY
·
2025
·
IF 6.6
Lanqing Yang,Qiuya Li,Baoqing Bai,Tao Bo,Ligang Yu,Xu Jing,Shu Qin,Huilin Liu,Ying Zhang,Yukun Yang
Aflatoxin B1 (AFB1), a highly toxic mycotoxin prevalent in agricultural products, poses severe health threats. Addressing the critical need to mitigate AFB1-induced food safety risks, the primary objective of this study was to develop an accurate and ultrasensitive colorimetric/electrochemical (CL/EC) dual-mode aptasensor for AFB1 detection. To achieve this aim, we employed 3,3′,5,5′-tetramethylbenzidine (TMB) as a dual-function indicator. The catalytic oxidation of TMB generated oxidized oxTMB, concurrently producing colorimetric and electrochemical signal. Central to realizing the sensor's dual functionality, BSA@HOF-101 nano-enzymes were generated by in situ encapsulation of bovine serum albumin (BSA) in hydrogen-bonded organic frameworks (HOFs), which were indispensable enzyme mediators for the dual function of TMB. To specifically detect AFB1, BSA@HOF-101 was coupled with aptamer (Apt). The CL/EC dual-mode aptamer sensor offered detection of AFB1 across a concentration range from 10 −11 to 10 −7 g/mL. In addition, the sensor was successfully applied to the detection of AFB1 in corn and peanut samples with recoveries as high as 97.4 %–104.0 %. Collectively, this work established a robust analytical strategy with a dual-mode readout, providing a promising solution for the rapid and precise on-site monitoring of AFB1 contamination.
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