CHEMOSPHERE
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2019
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IF 5.108
Huimin Wang,Pingxiao Wu,Juan Liu,Shanshan Yang,Bo Ruan,Saeed Rehman,Liangting Liu,Nengwu Zhu
Cadmium (Cd) is a toxic heavy metal and its uptake by living organisms causes adverse effect, further resulting in cycle pollution of the biosphere. The specific regulatory mechanism between clays and microbes under Cd stress remains unclear. In this study, interface interactions among clays, microbes and Cd were confirmed. Comparative transcriptome was conducted to investigate how it regulated gene expression patterns of microbes ( Chryseobacterium sp. WAL2), which exposed to a series of gradient concentrations of Cd (16, 32, 64 and 128 μg mL −1 ) for 12 d in the presence and absence of clay montmorillonite (Mt) (16 g L −1 ). Cd was highly enriched by the unique interface interactions between Mt and bacteria (67.6–82.1%), leading to a more hostile environment for bacterial cells. However, Mt ultimately enhanced bacterial resistance to Cd stress by stimulating the mechanism of bacterial resistance; namely: (i) Mt increased genes expression connected with ion transport, enhancing the uptake of Cd; (ii) Mt stimulated genes expression related to efflux pump and positively regulated cellular oxidative stress (e.g., glutathione) and Cd accumulation (e.g., cysteine) processes. Further, genes expression related to intracellular metabolic processes was enforced, which supplied a driving force and accelerated electron transfer ; (iii) Mt improved genes expression involved in DNA replication and other biological processes (e.g., terpenoid backbone biosynthesis) to maintain bacterial vitality. Therefore, the study not only optimized a unique Cd resistance mechanism of Mt on Chryseobacterium sp., but also provided a novel insight for environmental mitigation of heavy metals from the perspective of molecular biology .
研究领域:
环境微生物学
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分子生物学
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重金属污染治理
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PubMed
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2022
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IF 10.753
Chao Guo,Lutz Ahrens,Stefan Bertilsson,Marco J.L. Coolen,Jianhui Tang
The impact of perfluoroalkyl and polyfluoroalkyl substances on microbial communities is challenging to investigate in situ because of the complexity and dynamics of natural ecosystems. In the present study, four microcosms were established to explore the impact of perfluorooctanoate (PFOA) on bacterial communities in riverine and marine settings. PFOA distribution between the aqueous and sedimentary phases fluctuated in both PFOA-amended and unamended control systems. PFOA was more rapidly partitioned into the sediment in marine than in riverine microcosms. Differences in iron concentration and salinity may influence PFOA exchange between water and sediment. In marine microcosms, the alpha diversity of bacterial communities was significantly correlated to PFOA concentration. PFOA tended to correlate more strongly with bacterial community composition in water than in sediment. At the whole system level, Lefse's analysis indicated Algoriphagus halophilus as biomarkers for PFOA exposure in both riverine and marine systems, and the family Flavobacteriaceae were also more abundant in the exposed systems. In terms of temporal variation (comparison between three time points in the systems), metastat analysis showed great variability of potential PFOA-sensitive bacteria at the genus level. As such, most PFOA-sensitive genera were transitory and variable and existed for a short term in different systems (river, sea, blank, and experiment) and phases. Compared with other PFOA-sensitive genera, we suggest that further research is carried out to explore the use of Limnobacter as a bioindicator for temporal monitoring of PFOA pollution.
研究领域:
环境科学
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微生物学
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PubMed
高影响因子
ISME Journal
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2021
·Vol. 15
·期 6
·1844-1857
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IF 10.302
Liu Qing,Li Wei,Liu Di,Li Lingyan,Li Jie,Lv Na,Liu Fei,Zhu Baoli,Zhou Yuguang,Xin Yuhua,Dong Xiuzhu
Bacteria that inhabit glaciers usually produce carotenoids. Here, we report that a group of zeaxanthin-producing glacial Flavobacterium exhibited light-promoted growth. Of the tested 47 strains, 45 showed increased growths but two died under illumination at 50 μmol photon m −2 s −1 . Light stimulation occurred mainly in either anoxic or nutrient-poor cultures, while the same levels of light promotion were found for that grown at 14 and 7 °C. Pigment assays identified overrepresentative zeaxanthin but trace retinal in the light promoted 45 strains, while flexirubin was exclusively in the light-lethal two. Genomic analysis revealed the gene cluster for zeaxanthin synthesis in the 45 strains, in which 37 strains also harbored the proteorhodopsin gene prd . Transcriptomic analysis found that light-induced expressions of both the zeaxanthin synthesis and proteorhodopsin genes. Whereas, deletion of the prd gene in one strain did not diminish light promotion, inhibition of zeaxanthin synthesis did. In comparison, no light promotion was determined in a glacier Cryobacterium luteum that produced a non-zeaxanthin-type carotenoid. Therefore, light stimulation on the glacial Flavobacterium is mostly likely related to zeaxanthin, which could provide better photoprotection and sustain membrane integrity for the organisms living in cold environments.
研究领域:
微生物学
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环境科学
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分子生物学
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DOI
PubMed
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2024
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IF 8.2
Hongyan Luo,Zhishuang Yang,Ting Lei,Caixia Li,Zuoyong Zhou,Mingshu Wang,Dekang Zhu,Pei Li,Anchun Cheng
Carbapenem resistance's global proliferation poses a significant public health challenge. The primary resistance mechanism is carbapenemase production. In this study, we discovered a novel carbapenemase, RATA, located on the chromosome of Riemerella anatipestifer isolates. This enzyme shares ≤52 % amino acid sequence identity with other known β-lactamases. Antimicrobial susceptibility tests and kinetic assays demonstrated that RATA could hydrolyze not only penicillins and extended-spectrum cephalosporins but also monobactams, cephamycins, and carbapenems. Furthermore, its activity was readily inhibited by β-lactamase inhibitors. Bioinformatics analysis revealed 46 bla RATA -like genes encoding 27 variants in the NCBI database, involving 21 different species, including pathogens, host-associated bacteria, and environmental isolates. Notably, bla RATA -positive strains were globally distributed and primarily collected from marine environments. Concurrently, taxonomic analysis and GC content analysis indicated that bla RATA orthologue genes were predominantly located on the chromosomes of Flavobacteriaceae and shared a similar GC content as Flavobacteriaceae . Although no explicit mobile genetic elements were identified by genetic environment analysis, bla RATA-2 possessed the ability of horizontal transfer in R. anatipestifer via natural transformation. This work's data suggest that RATA is a new chromosome-encoded class A carbapenemase, and Flavobacteriaceae from marine environments could be the primary reservoir of the bla RATA gene.
研究领域:
微生物学
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分子生物学
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传染病学
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公共卫生
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PubMed
高影响因子
BIOCHEMICAL ENGINEERING JOURNAL
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2021
·
IF 3.978
Tao Xu,Teng Liu,Dawei Jiang,Ziyue Yuan,Xiaoqiang Jia
Persistent organic pollutants (POPs) mainly including polycyclic aromatic hydrocarbons (PAHs), polychlorinated biphenyls (PCBs), and organochlorine pesticides (OPs), have long-term harmful effects on human health and the environment. In this study, a natural consortium BP-W was attained from oil-contaminated soil using biphenyl as the sole carbon source, which is a relatively simple PAH and can form PCBs under certain catalytic conditions. PCR-DGGE techniques were used to analyze the structure of the consortium BP-W. The results showed that this consortium was composed of Chryseobacterium BP-1, Pseudomonas putida BP-2, Stenotrophomonas BP-3, Achromobacter BP-4, and an uncultured bacterium. The consortium BP-W degraded 99.88 % of 1 g L −1 biphenyl in 72 h. The degradation ratios of 100 mg L −1 naphthalene, phenanthrene, pyrene, and PCB77 in 5 days were 100 %, 97.54 %, 42.26 %, and 26.73 %, respectively. Notably, the consortium BP-W exhibited a high tolerance for these POPs. In the binary-substrate and ternary-substrate systems, the consortium BP-W was still able to rapidly degrade organic substrates. The results of gas chromatography-mass spectrometry showed that biphenyl was completely degraded by BP-W and entered the TCA cycle through the protocatechuic acid pathway, and no other highly toxic metabolites were produced during the degradation process. Thus, this consortium BP-W may have potential utility for the environmental-friendly and cost-effective bioremediation of POPs.
研究领域:
环境科学
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微生物学
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生物技术
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DOI
高影响因子
METABOLIC ENGINEERING
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2019
·
IF 7.808
Wan-Ju Sun,Li Wang,Hao-Hao Liu,Yong-Jun Liu,Yu-Hong Ren,Feng-Qing Wang,Dong-Zhi Wei
The conversion of sterols to steroid synthons by engineered mycobacteria comprises one of the basic ways for the production of steroid medications in the pharmaceutical industry. Here, we revealed that high amounts of reactive oxygen species (ROS) generate during the conversion process of sterols, which impairs the cell viability of mycobacterial cells and thus hinders the conversion of sterols to steroid synthons. Accordingly, the endogenous antioxidants for detoxifying ROS in mycobacteria, ROS scavenging enzymes and low molecular weight thiols, were examined. The results revealed that three antioxidants, catalase (CAT), mycothiol (MSH), and ergothioneine (EGT), demonstrated efficacy toward neutralizing the excessive ROS produced during sterol metabolism . CAT overexpression or MSH or EGT augmentation enhanced the conversion of phytosterols to 22-hydroxy-23,24-bisnorchol-4-ene-3-one (4-HBC) by 18.9%, 23.8%, and 32.1%, respectively, and also enhanced the cell viability, indicating the benefits of these antioxidants in reducing ROS-induced stress. Further combinatorial augmentation of CAT, MSH, and EGT demonstrated enhanced effects toward intracellular ROS scavenging, resulting in 54.2% greater cell viability and 47.5% enhancement in 4-HBC production. These findings indicated that the excessive ROS induces cell stress, in turn limiting the conversion of sterols, whereas neutralization of the excessive ROS by combined control of CAT, MSH, and EGT serves as an effective strategy to boost the conversion productivity of sterols to steroid synthons.
研究领域:
药学
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微生物学
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生物技术
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生物化学
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DOI
PubMed
高影响因子
CHEMOSPHERE
·
2023
·
IF 8.8
Huanjun Zhang,Yanan Yu,Yi Li,Li Lin,Chi Zhang,Wenlong Zhang,Longfei Wang,Lihua Niu
Intimately coupled photocatalysis and biodegradation (ICPB) is a promising technology to remove refractory contaminants from water. The key to successful ICPB is a carrier capable of accumulating biofilm and adhering photocatalyst firmly. Herein, BC/g-C 3 N 4 was prepared into a three dimensional porous hydrogel and used as a carrier in ICPB system for the first time. Degradation experiments revealed that the removal rate of tetracycline hydrochloride (TCH) in water by the ICPB system was 96.0% after 10 h, which was significantly higher than that by the photocatalysis (PC, 76.3%), biodegradation (B, 32.5%), adsorption (AD, 17.2%), and photolysis (P, 5.0%) systems. Photo-electrochemical tests confirmed that ICPB system had superior electron transfer ability between photocatalysts and microorganisms . The removal efficiency of COD proved that microorganisms played an important role in the mineralization process of TCH by the ICPB system. After the ICPB degradation experiment, microorganisms maintained high activity and Pseudomonas , Burkholderiaceae and Flavobacterium which had TCH degradation or electron transport ability, were enriched. In conclusion, the novel ICPB carrier overcame shortcomings of the traditional ICPB carrier and the novel ICPB system had superior degradation performance for TCH. This study provided a possible method to promote the practical application of ICPB technology .
研究领域:
环境科学
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水处理
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光催化
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生物降解
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微生物学
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DOI
PubMed
高影响因子
BIORESOURCE TECHNOLOGY
·
2019
·
IF 6.669
Fansheng Meng,Weiwei Huang,Dongfang Liu,Yingxin Zhao,Wenli Huang,Zhongfang Lei,Zhenya Zhang
In this study a continuous flow reactor (CFR) was employed to compare the feasibility of bacterial aerobic granular sludge (AGS-CFR) and algal-bacterial granular sludge (ABGS-CFR) for treating 1–4% saline wastewater. High salinity was found to enhance algae growth in ABGS-CFR, which exhibited slightly higher total nitrogen and phosphorus removal efficiencies at 1–3% salinity. ABGS-CFR maintained good granular stability at 1–4% salinity, while AGS-CFR gradually disintegrated at 4% salinity with 39.3% less accumulation of alginate-like exopolysaccharides in the extracellular polymeric substances. Indole-3-acetic acid (IAA) and superoxide dismutase (SOD) analysis suggested that bacteria and algae ( Nitzschia ) in ABGS-CFR formed a good symbiotic relationship under high salinity conditions, achieving rapid algae growth and 2 times lipid production. High salinity was conducive to enriching Halomonas and Nitzschia but unfavorable for Nitrosomonas and Flavobacterium . Results from this study could provide useful information on interactions between bacteria and algae in ABGS-CFR for its future practical application.
研究领域:
环境工程
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废水处理
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微生物学
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生物技术
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DOI
高影响因子
CHEMOSPHERE
·
2023
·
IF 8.8
Zizhang Guo,Yan Kang,Haiming Wu,Mei Li,Zhen Hu,Jian Zhang
Polycyclic aromatic hydrocarbon (PAHs) are persistent organic pollutants and pose high risk in aquatic environment. The utilization of biochar is a strategy for PAHs-contaminated remediation but is challenging due to the adsorption saturation and reoccurrence of PAHs desorbed back into water. In this study, iron (Fe) and manganese (Mn) were provided as electron acceptors for biochar modification to enhance anaerobic biodegradation of phenanthrene (Phe). Results revealed that, the Mn(Ⅳ) and Fe(Ⅲ) modification improved the removal of Phe by 24.2% and 31.4% than that of biochar, respectively. Additionally, nitrate removal was improved by 19.5% with Fe(Ⅲ) amendment. The Mn-and Fe-biochar decreased Phe contents by 8.7% and 17.4% in sediment, 10.3% and 13.8% in biochar than that of biochar. Much higher DOC contents were observed with Mn- and Fe-biochar, which provided bioavailable carbon source for microbes and contributed to microbial degradation of Phe. The greater degree of humification , higher proportions of humic and fulvic acid like components in metallic biochar participated in electron transport and further enhancing the degradation of PAHs. Microbial analysis proved the high abundance of Phe-degrading bacteria (e.g. PAH-RHD α , Flavobacterium and Vibrio ), nitrogen removal microbes (e.g. amoA , nxrA , and nir ), Fe and Mn bioreduction or oxidation (e. g. Bacillus , Thermomonas , Deferribacter ) with metallic biochar. Based on the results, the Fe and Mn modification, especially Fe-modified biochar provided well performance for PAHs removal in aquatic sediment.
研究领域:
环境科学
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生物修复
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水生生态学
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微生物学
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DOI
PubMed
高影响因子
Journal of Environmental Chemical Engineering
·
2023
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IF 7.7
Zihang Ma,Zuhan Ge,Kexuan Liu,Cong Wang,Tao Wu,Jibiao Zhang
In this study, calcium peroxide (CaO 2 ) was added to two types of water to remove turbidity . The effects of CaO 2 on water quality, Ca content and microbial communities were also examined. The results showed that CaO 2 significantly improved the transparency of highly turbid water induced by algae. Compared with the control group, 5, 10, and 20 mg/L CaO 2 reduced the turbidity by 56.80%, 81.66%, and 82.54%, respectively. Considering the actual effect and cost, 10 mg/L CaO 2 was the recommended dosage. Furthermore, the addition of CaO 2 increased the degree of humification in the water and Ca content in the sediment. However, the addition of CaO 2 did not significantly increase the concentration of Ca 2+ in the water. Additionally, CaO 2 first decreased the richness and diversity of the bacterial and fungal communities. However, this effect gradually disappeared over time. The abundance of cyanobacteria decreased to 0 after 168 h. Actinobacteria and Compilobacteria were also inhibited by CaO 2 . At the genus level, Flavobacterium , Sphingomonas , Pseudomonas , and Limnohabitans , which occupied key nodes, were tolerant to CaO 2 , and CaO 2 had only a minor impact on the microbial ecological networks. Moreover, the eukaryotic rotifer community was destroyed. CaO 2 increased the abundance of chemotrophic heterotrophic functional groups but decreased that of the functional groups. These findings provide guidance for the use of CaO 2 to improve water turbidity and water ecological restoration.
研究领域:
环境科学
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水处理
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微生物学
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生态学
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DOI
高影响因子
ENVIRONMENTAL POLLUTION
·
2022
·
IF 9.988
Qingyun Ma,Hao Tan,Jinlong Song,Miaomiao Li,Zhiye Wang,Rebecca E. Parales,Lin Li,Zhiyong Ruan
This study aims to investigate the effects of long-term nicosulfuron residue on an herbicide factory ecosystem. High-throughput sequencing was used to investigate the environmental microbial community structure and interactions. The results showed that the main contributor to the differences in the microbial community structure was the sample type, followed by oxygen content, pH and nicosulfuron residue concentration. Regardless of the presence or absence of nicosulfuron, soil, sludge , and sewage were dominated by groups of Bacteroidetes, Actinobacteria, and Proteobacteria. Long-term exposure to nicosulfuron increased alpha diversity of bacteria and archaea but significantly decreased the abundance of Bacteroidetes and Acidobateria compared to soils without nicosulfuron residue. A total of 81 possible nicosulfuron-degrading bacterial genera, e.g., Rhodococcus , Chryseobacterium , Thermomonas , Stenotrophomonas , and Bacillus , were isolated from the nicosulfuron factory environmental samples through culturomics. The co-occurrence network analysis indicated that the keystone taxa were Rhodococcus , Stenotrophomonas , Nitrospira , Terrimonas , and Nitrosomonadaceae_MND1. The strong ecological relationship between microorganisms with the same network module was related to anaerobic respiration, the carbon and nitrogen cycle , and the degradation of environmental contaminants. Synthetic community (SynCom), which provides an effective top-down approach for the critical degradation strains obtained, enhanced the degradation efficiency of nicosulfuron. The results indicated that Rhodococcus sp. was the key genus in the environment of long-term nicosulfuron exposure.
研究领域:
环境科学
·
微生物学
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生态学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）