PHYTOMEDICINE
·
2021
·
IF 5.34
Li-Wei Wang,Xue-Ying Cui,Jiang-Feng He,Shen Duan,Chun-Rui Liu,Cheng-Bin Shan,Yu Wang,Chao-Mei Ma
Background Hydroxysafflor yellow A (HSYA) from the flower of Carthamus tinctorius (Safflower) has been reported to have various pharmacological effects. However, little is known about the bioactivities of other chemical constituents in Safflower and the relationship between enhancement of blood circulation and hepatoprotection by HSYA. Purpose The present research was to evaluate the antithrombotic and hepatoprotective activities of HSYA and C, examine their mechanisms of actions, including influence on the excretion velocity of acetaminophen, and the relationship between the antithrombotic, hepatoprotective, and other bioactivities. Methods The hepatoprotective activities were examined by acetaminophen (APAP)-induced zebrafish toxicity and carbon tetrachloride (CCl 4 )-induced mouse liver injury. The concentrations of APAP in zebrafish and APAP that was excreted to the culture media were quantified by UHPLC-MS. The anti-thrombosis effect of HSYA and C were examined by the phenylhydrazine (PHZ)-induced zebrafish thrombosis. Results HSYA and HSYC showed robust protection on APAP-induced toxicity and PHZ-induced thrombosis. The hepatoprotective effects of HSYA and C were more potent than that of the positive control, acetylcysteine (61.7% and 58.0%, respectively, vs. 56.9% at 100 µM) and their antithrombosis effects were more robust than aspirin (95.1% and 86.2% vs. 52.7% at 100 µM). HSYA and C enhanced blood circulation, rescued APAP-treated zebrafish from morphological abnormalities, and mitigated APAP-induced toxicity in liver development in liver-specific RFP-expressing transgenic zebrafish. HSYC attenuated CCl 4 -induced mouse liver injury and regulated the levels of HIF-1α, iNOS, TNF-α, α-SMA, and NFκB in liver tissues. HSYA was also protective in a dual thrombotic and liver toxicity zebrafish model. By UHPLC-MS, HSYA accelerated the excretion of APAP. Conclusion HSYA and C are the bioactive constituents of Safflower that are responsible for the herbal drug's traditional use in promoting blood circulation to remove blood stasis. Safflower and its chalcone constituents may protect from damage due to exogenous or disease-induced endogenous toxins by enhancing the excretion velocity of toxins.
研究领域:
药理学
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毒理学
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药物化学
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动物模型
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DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2021
·
IF 7.514
Xin Tong,Jiehong Yang,Yu Zhao,Haofang Wan,Yu He,Ling Zhang,Haitong Wan,Chang Li
Natural deep eutectic solvents (NaDESs) are promising green alternatives to conventional solvents widely applied in the extraction of natural products due to their physical and chemical superiorities. In present study, 22 NaDESs consisted from food grade ingredients were screened in ultrasonic assisted extraction (UAE) of bioactive compounds from safflower. The oral bioavailabilities of hydroxysafflor yellow A (HSYA) and anhydrosafflor yellow B (ASYB) in the extracts were then investigated in SD rats with the help of HPLC-MS technique. The results revealed that l -proline-acetamide ( l -Pro-Am) was an effective solvent with the yields of HSYA and ASYB at 32.83 and 8.80 mg/g. Pharmacokinetic studies revealed that the blood level of HSYA and ASYB were significantly higher after oral administration of l -Pro-Am extract than that of aqueous extract. Especially, the relative bioavailabilities (to aqueous extract) of HSYA and ASYB were calculated 183.5% and 429.8%.
研究领域:
药理学
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化学工程
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分析化学
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DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2018
·
IF 4.946
Xi-Zhe Fu,Qing-An Zhang,Bao-Shan Zhang,Pei Liu
As a potential novel technique in the wine-making industry, ultrasound has received considerable attention. In this paper, a model wine system was constructed to investigate the effect of ultrasonic irradiation on the formation of xanthylium cation pigment derived from the (+)-catechin and the glyoxylic acid, an oxidation product of tartaric acid, so as to explore the changing mechanism of the wine color mediated by ultrasound. The results indicate that the long-term ultrasonic treatment can enhance the formation of the xanthylium cation pigment in the model wine, which is attributed to free radicals triggered by ultrasound, meanwhile, iron ions and oxygen can influence the ultrasonic effect. All these results suggest that ultrasound is promising to be employed in regulating the formation of the yellow pigment during wine aging thus modifying the wine organoleptic characteristic.
研究领域:
食品科学
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生物化学
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DOI
PubMed
高影响因子
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
·
2021
·
IF 4.556
Tang Liqin,Liu Haocheng,Wen Jing,Xu Yujuan,Tian Wenni,Li Lu,Yu Yuanshan,Lin Xian,Fu Manqin
The acquisition of yellow extract (YE) from gardenia ( Gardenia jasminoides ) fruit can promote the application of natural pigment. This study aims to establish an effective method for extracting YE from gardenia fruit and to evaluate the properties of YE. First, the seven compounds in YE obtained by ultrahigh-pressure extraction (UPE) were identified by LC-TOF-MS/MS. The effects of traditional extraction (TE), ultrasonic extraction (UE), and UPE methods on the yield of YE were then investigated. The results showed that the extraction yield of UPE was 7.73 ± 0.89 mg/g DW under the optimized conditions (47.78% ethanol concentration, 114.95 s time, and 18.08 mL/g liquid–solid ratio), which was higher than those of UE (4.81 ± 0.23 mg/g DW) and TE (3.55 ± 0.21 mg/g DW). Finally, the YE stability of UPE was studied. The results showed that the YE stability of UPE was similar to that of the TE group, and both of which were greatly affected by higher temperature (60–100 °C), ultraviolet radiation, alkalinity , and Fe 3+ . Meanwhile, YE of UPE had better stability under ultraviolet radiation and alkalinity in comparison with the TE group. Therefore, UPE is a potential method for YE extraction from gardenia fruit.
研究领域:
食品科学
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植物化学
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产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2021
·
IF 7.514
Jinshuang Wang,Qin Ye,Ningxiang Yu,Weiwei Huan,Jingliang Sun,Xiaohua Nie,Xianghe Meng
In this work, a robust method for the separation of gardenia yellow and geniposide from gardenia fruit was developed based on a molecularly imprinted solid phase extraction (MISPE) procedure. First, hydrophilic molecularly imprinted microspheres (HMIMs) were prepared using gardenia yellow as the template via reversible addition fragmentation chain transfer (RAFT) precipitation polymerization. The resultant HMIMs demonstrated the multiresponsiveness to pH, temperature, and magnetism, achieving controllable uptake and release of gardenia yellow and easy recovery by external magnets. Meanwhile, the HMIMs possessed high adsorption capacity, fast binding kinetics, specific recognition, and reusability. Finally, a MISPE approach using HMIMs as adsorbent was developed for extraction of gardenia yellow and purification of geniposide after optimization of the adsorption and elution conditions. Thus, efficient separation of gardenia yellow and geniposide with relative purities of 99.77 ± 0.05% (94.04 ± 0.10% recovered) and 94.50 ± 0.62% (95.40 ± 0.86% recovered), respectively, was achieved.
研究领域:
分析化学
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植物化学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
·
2022
·Vol. 103
·期 4
·1846-1855
·
IF 4.125
Tianqi Li,Jinzhe Li,Yuxuan Huang,Abdul Qayum,Zhanmei Jiang,Zhenmin Liu
Background The protein–polyphenol interaction mechanism has always been a research hotspot, but their interaction is affected by heat treatment, which is widely applied in food processing. Moreover, the effects of microwave or water-bath heating on the protein–polyphenol interaction mechanism have been not clarified. The pasteurization condition (65 °C, 30 min) was selected to compare the effects of microwave or water bath on binding behavior, structure, and cell proliferation between α -lactalbumin ( α -LA) and safflower yellow (SY), thus providing a guide for the selection of functional dairy processing conditions. Results Microwave heat treatment of α -LA–SY resulted in stronger fluorescence quenching than that of conventional heat treatment. Moreover, the binding constant K a of all α -LA–SY samples was augmented significantly after microwave or water bath treatment, and microwave-heated α -LA–SY showed the maximum K a . Fourier transform infrared spectroscopy showed that microwave heating resulted in more ordered structures of α -LA into its disordered structures than water bath heating. However, the ferric reducing antioxidant power and chroma value of α -LA–SY were more reduced by microwave heating than by water bath heating. Moreover, microwave heating facilitated the cell proliferation of α -LA–SY compared with water bath treatment. Conclusion It was demonstrated that microwave heating promoted interaction between α -LA and SY more than water bath heating did. Microwave heat treatment was a safe and effective way to enhance the binding affinity of α -LA to SY, being a potential application in food industry. © 2022 Society of Chemical Industry.
研究领域:
食品科学
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生物化学
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DOI
PubMed
高影响因子
JOURNAL OF FOOD BIOCHEMISTRY
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2017
·Vol. 42
·期 2
·e12475
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IF 1
Benguo Liu,Jiangwei Liu,Chunling Zhang,Jiechao Liu,Zhonggao Jiao
During apple processing, phloridzin, the main flavonoid in apples, can be oxidized to the yellow pigment, which contributes to the color of apple juice and ciders. However, there was no report about the bioactivities of this yellow pigment. In this study, the oxidation of phloridzin catalyzed by tyrosinase from mushroom was investigated. The main oxidation product with bright yellow color was purified and identified as the phloridzin oxidation product j (POPj) based on electrospray ionization mass spectrometry (ESI-MS) and 13 C nuclear magnetic resonance ( 13 C-NMR) spectroscopy. In the chemical and cellular system, POPj exhibited the higher peroxyl radical-scavenging activity than phloridzin. Our results suggested that POPj was a kind of potential food yellow pigment with antioxidant activity, which partly explained health-promoting functions of apple juices and ciders. Practical applications In view of the potential toxicity of the artificial food yellow pigment, the need for natural and safe yellow pigment is increasing. In this study, the enzymatic preparation of the yellow pigment derived from phloridzin was investigated. Its antioxidant activity was first reported. This study can prompt the application of this new yellow pigment in food industry.
研究领域:
食品科学
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生物化学
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农业化学
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DOI
高影响因子
Pharmaceuticals
·
2022
·Vol. 15
·期 11
·1394
·
IF 5.215
Tingting Li,Dong Han,Zhongxian Li,Mengqi Qiu,Yuting Zhu,Kai Li,Jiawei Xiang,Huizhen Sun,Yahong Shi,Tun Yan,Xiaoli Shi,Qiang Zhang
Idiopathic pulmonary fibrosis is a fatal interstitial disease characterized by fibroblast proliferation and differentiation and abnormal accumulation of extracellular matrix, with high mortality and an increasing annual incidence. Since few drugs are available for the treatment of pulmonary fibrosis, there is an urgent need for high-efficiency therapeutic drugs and treatment methods to reduce the mortality associated with pulmonary fibrosis. The interstitium, a highly efficient transportation system that pervades the body, plays an important role in the occurrence and development of disease, and can be used as a new route for disease diagnosis and treatment. In this study, we evaluated the administration of hydroxysafflor yellow A phytosomes via intervaginal space injection (ISI) as an anti-pulmonary fibrosis treatment. Our results show that this therapeutic strategy blocked the activation of p38 protein in the MAPK-p38 signaling pathway and inhibited the expression of Smad3 protein in the TGF-β/Smad signaling pathway, thereby reducing secretion of related inflammatory factors, deposition of collagen in the lungs of mice, and destruction of the alveolar structure. Use of ISI in the treatment of pulmonary fibrosis provides a potential novel therapeutic modality for the disease.Keywords:intervaginal space injection;pulmonary fibrosis;hydroxysafflor yellow A;phytosome;interstitial treatment
研究领域:
药理学
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肺科学
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分子生物学
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DOI
PubMed
高影响因子
Food Bioscience
·
2025
·
IF 5.9
Kaize Zheng,Xueling Qin,Lingchun Meng,Yusheng Liang,Junwei Qin,Zhenqiang Wu,Zhenfeng Huang
Natural Monascus yellow pigments (MYPs) have potential in the food industry due to their safety, but their application is limited by low biological activity and stability. Novel MYPs complexes, Monascus yellow pigments-tannic acid (MYPs-TA), were synthesized by spontaneously incorporating TA through intermolecular co-pigmentation in this work. Structural analysis revealed that multiple interactions contributed to the formation of complexes, including hydrogen bonding, π-π stacking, and hydrophobic interactions. The MYPs-TA exhibited characteristic ultraviolet signals of both MYPs (380 nm) and TA (276 nm), and the crystalline structure of MYPs transitioned to an amorphous form. These phenomena confirmed the tight combination of MYPs and TA. Color tonality, physicochemical stability as well as antimicrobial and antioxidant activities of MYPs were significantly improved after interaction with TA. Besides, MYPs-TA exhibited optimal stability and functionality in high-temperature processed foods such as baked goods or pasteurized products. This study provides a novel approach for synthesizing functional MYPs complexes with superior stability.
研究领域:
食品化学
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食品科学
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天然产物化学
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功能食品
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材料科学
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产品关联
DOI
高影响因子
Polymers
·
2025
·Vol. 17
·期 14
·1900
·
IF 4.9
Guoying Zhou,Yujia Yan,Linh Nguyen,Jiangkai Fan,Xiao Zhang,Li Gan,Tingzi Yan,Haitong Wan
Traumatic brain injury (TBI) leads to severe neurological dysfunction, disability, and even death. Surgical intervention and neurorehabilitation represent the current clinical management methods, yet there remains no effective treatment for recovery after TBI. Post-traumatic hyperinflammation and vascular injury are the key therapeutic challenges. Therefore, a novel-designed multifunctional HT/SAA/HSYA hydrogel based on hyaluronic acid (HA) co-loaded with salvianolic acid A (SAA) and hydroxysafflor yellow A (HSYA) was developed in order to simultaneously target inflammation and vascular injury, addressing key pathological processes in TBI. The HT hydrogel was formed through covalent cross-linking of tyramine-modified HA catalyzed by horseradish peroxidase (HRP). Results demonstrated that the HT hydrogel possesses a porous structure, sustained release capabilities of loaded drugs, suitable biodegradability, and excellent biocompatibility both in vitro and in vivo. WB, immunofluorescence staining, and PCR results revealed that SAA and HSYA significantly reduced the expression level of pro-inflammatory cytokines (IL-1β and TNF-α) and inhibited M1 macrophage polarization through the suppression of the TLR4/NF-κB inflammatory pathway. In vivo experiments confirmed that the HT/SAA/HSYA hydrogel exhibited remarkable pro-angiogenic effects, as evidenced by increased expression of CD31 and α-SMA. Finally, H&E staining showed that the HT/SAA/HSYA hydrogel effectively reduced the lesion volume in a mouse TBI model, and demonstrated more pronounced effects in promoting brain repair at the injury site, compared to the control and single-drug-loaded hydrogel groups. In conclusion, the HT hydrogel co-loaded with SAA and HSYA demonstrates excellent anti-inflammatory and pro-angiogenic effects, offering a promising therapeutic approach for brain repair following TBI.
研究领域:
神经外科学
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生物材料学
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免疫学
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药理学
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再生医学
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2021
·
IF 6.953
Qian Zhang,Luping Gu,Yujie Su,Cuihua Chang,Yanjun Yang,Junhua Li
The aim of the present study was to develop soy protein isolate (SPI) and κ-carrageenan (KC) composite hydrogels as a delivery system for hydrophilic compounds. The pigment of monascus yellow was used as a model. A systematic study was performed to characterize the rheological, textural, microstructural properties and in vitro digestion release profile of monascus yellow of the composite gels. The results of power law modeling, electrophoresis patterns and fourier transform infrared spectroscopy (FTIR) confirmed that non-covalent interactions were involved in the formation of SPI/KC composite hydrogels. Compared to pure κ-carrageenan hydrogels, the incorporation of SPI could promote the formation of tougher, more uniform and compact composite gels with sustained-release property. In addition, the release behaviors of monascus yellow entrapped in the hydrogel network can be well described by the Ritger-Peppas mathematical model. Overall, our study provided a promising strategy to enhance the sustained release performance of hydrogels in digestive conditions.
研究领域:
食品科学
·
生物材料学
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DOI
高影响因子
MOLECULES
·
2019
·Vol. 24
·期 6
·1128
·
IF 3.06
Yanhua Tu,Beixuan He,Songyan Gao,Dandan Guo,Xinlei Jia,Xin Dong,Meili Guo
Background: Flavonoids with various structures play a vital role in plant acclimatization to varying environments as well as in plant growth, development, and reproduction. Exogenous applications of ethylene and 1-aminocyclopropane carboxylic acid (ACC), could affect the accumulation of flavonoids. Very few attempts have been made to investigate the effect of 1-aminocyclopropane carboxylic acid oxidase (ACO), a unique enzyme that catalyzes ACC to ethylene, on genes and metabolites in the flavonoid biosynthetic pathway. In this study, two ACOs in safflower (CtACOs) were cloned, and then transgenic safflower with overexpressed CtACO1 was generated through the Agrobacterium-mediated floral dipping method. Results: CtACO1 and CtACO2 were both characterized by the 2-oxoglutarate binding domain RxS and the ferrous iron binding site HxDxnH as ACOs from other plants. However, the transcript levels of CtACO1 in flowers at stages I, II, III, and IV were all higher than those of CtACO2. At the cellular level, by using electroporation transformation, CtACO1 was found to be localized at the cytomembrane in onion epidermal cells. CtACO1 overexpression had varying effects on genes involved in the ethylene and flavonoid biosynthetic pathways. The metabolites analysis showed that CtACO1 overexpression lines had a higher accumulation of quercetin and its glycosylated derivatives (quercetin 3-β-d-glucoside and rutin). In contrast, the accumulation of quinochalcones (hydroxysafflor yellow A and carthamin), kaempferol glycosylated derivatives (kaempferol-3-O-β-rutinoside and kaempferol-3-O-β-d-glucoside), apigenin, and luteolin in CtACO1 overexpression lines were decreased. Conclusion: This study confirmed the feasibility of applying the floral dipping method to safflower and showed a novel regulatory effect of CtACO1 in the flavonoid biosynthetic pathway. It provides hypothetical and practical groundwork for further research on regulating the overall metabolic flux of flavonoids in safflower, particularly hydroxysafflor yellow A and other quinochalcones, by using appropriate genetic engineering strategies.Keywords:safflower (Carthamus tinctoriusL.);floral dipping method;CtACO1 overexpression;flavonoid accumulation
研究领域:
植物生理学
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分子遗传学
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代谢工程
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DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）