CARBON
·
2020
·
IF 8.821
Chengfei Zhao,Lina Wu,Xuewen Wang,Shaohuang Weng,Zhipeng Ruan,Qicai Liu,Liqing Lin,Xinhua Lin
In this work, quaternary ammonium carbon quantum dots (QCQD) was successfully synthesized with 2,3-epoxypropyltrimethylammonium chloride and diallyldimethylammonium chloride by a simple green “one-pot” method. QCQD was demonstrated to have satisfactory antibacterial activity against gram-positive bacteria, including Staphylococcus aureus , methicillin-resistant Staphylococcus aureus (MRSA), Staphylococcus epidermidis , Listera monocytogenes and Enterococcus faecalis . QCQD was used to treat MRSA-infected pneumonia in mice, and could promote the regression of pulmonary inflammation in lung of mice. In vivo and in vitro toxicity tests also confirmed that QCQD had acceptable biocompatibility. There is no doubt that all the experimental results confirmed that QCQD is a promising antimicrobial agent for treating infections of gram-positive bacteria. The antibacterial mechanism of QCQD was analyzed and confirmed by the quantitative proteomics based tandem mass tags, revealing that QCQD might interfere with the protein translation, posttranslational modification and protein turnover in bacterial cells by mainly acting on ribosomes and upregulating RNA degradation related proteins. The application of proteomics extended the research on antibacterial mechanism from single target to protein network and realized the research and analysis on multiple targets of bacteria treated by QCQD, providing a new idea and strategy for the development of efficient bactericidal weapon against drug-resistant bacteria.
研究领域:
微生物学
·
药理学
·
蛋白质组学
✓ 已验证
产品关联
DOI
高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
·
2019
·
IF 3.973
Chengfei Zhao,Xuewen Wang,Lina Wu,Wen Wu,Yanjie Zheng,Liqing Lin,Shaohuang Weng,Xinhua Lin
Antimicrobial resistance is becoming more and more serious and has become a potential hazard to human life and health. The fabrication of some new antibacterial substances against resistant bacteria is demanded. With the wide application and research of carbon nanomaterials, nitrogen-doped carbon quantum dots (NCQDs) were synthesized by a one-step chemical route herein. The particle size of NCQDs in the range of 2–5 nm were characterized by transmission electron microscopy (TEM), atomic force microscopy, and dynamic light scattering. The functional groups and optical properties of NCQDs were investigated by UV–vis absorption spectroscopy, X-ray photoelectron spectroscopy and Fourier transform infrared spectroscopy. Disk-diffusion tests showed that the NCQDs had specific antibacterial activity against Staphylococcus . TEM showed that the NCQDs could destroy the cell structure of Staphylococcus aureus and methicillin-resistant Staphylococcus aureus (MRSA) but could not combat Escherichia coli . The antibacterial mechanism may be that positively charged NCQDs firstly interacted with the negatively charged bacteria, and then specifically anchored on some specific sites on the surface of Staphylococcus . The NCQDs were applied to treat wounds infected with MRSA and showed the same therapeutic effect as vancomycin. Photomicrographs of hematoxylin-eosin-stained histological sections showed that the NCQDs at concentrations effectively killing S. aureus and MRSA caused negligible toxicity to the main rat organs, including heart, liver, spleen, lung, and kidney. Thus, the NCQDs can be developed as a promising antibacterial agent for Staphylococcus . And the NCQDs are likely to treat local infections caused by Staphylococcus clinically, especially S. aureus and MRSA .
研究领域:
生物医学
·
纳米材料科学
·
微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2014
·
IF 5.825
Lihong Li,Zhao Li,Wen Shi,Xiaohua Li,Huimin Ma
A highly sensitive and selective near-infrared (NIR) fluorescent probe, (E)-2-(2-(6-((2-carboxy-8-oxo-7-(2-phenylacetamido)-5-thia-1-azabicyclo[4.2.0]oct-2-en-3-yl)methoxy)-2,3-dihydro-1H-xanthen-4-yl)vinyl)-3,3-dimethyl-1-propyl-3H-indol-1-ium (1), is developed for the determination of β-lactamase. The probe is designed and synthesized by incorporating the specific substrate (cephalosporin) of β-lactamase into a stable hemicyanine skeleton. The fluorescence of 1 itself is very weak due to the alkylation of the hydroxyl group of the hemicyanine fluorophore; however, β-lactamase can selectively react with its substrate (β-lactam ring) in the probe, thereby causing a spontaneous fragmentation. This action leads to the release of the fluorophore and a large fluorescence enhancement at 707 nm (λex = 680 nm). Under the optimized conditions, the fluorescence response of probe 1 is directly proportional to the concentration of β-lactamase in the range of 0.05–2 nM, with a detection limit of 0.02 nM. The validity of the probe has been confirmed by determining β-lactamase in human urine samples in comparison with that determined by iodimetry. Moreover, by taking advantage of its high sensitivity and NIR emission feature, the probe has also been utilized to image β-lactamase in three types of Staphylococcus aureus, including methicillin-resistant S. aureus ATCC BAA44, penicillin-resistant strain ATCC 11632, and penicillin-susceptible strain ATCC 29213, which clearly reveals the significantly different expression levels of β-lactamase in these S. aureus.
研究领域:
微生物学
·
生物医学工程
·
药物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF ALLOYS AND COMPOUNDS
·
2021
·
IF 5.316
Pu Fan,Zefeng Xu,Qi Luo,Zongjie He,Yuhui Chen,Qiqi Miao,Cong Huang,Xiaoguang Liu,Ling Li
Phosphor-converted white LEDs are realized by combining blue InGaN chips with yellow and red emitting materials. Due to the lack of efficient red phosphor materials, we can design a broadband yellow phosphor material containing as many red components as possible. We found an abnormal broadband yellow-emitting Ba 2 CaB 2 Si 4 O 14 :Eu 2+ borosilicate phosphor inspired by natural minerals, which has been synthesized by traditional high temperature solid-state method. The structural information and photoluminescence characteristics of Ba 2 CaB 2 Si 4 O 14 :Eu 2+ were characterized in detail, and applied to the fabrication of white LED devices. Excited by near ultraviolet light at 365 nm, it displays a wide emission band from 400 to 800 nm centered on the 595 nm peak. White LED devices were fabricated by physically mixing the yellow phosphor Ba 2 CaB 2 Si 4 O 14 :Eu 2+ and synthesized blue phosphor BaMgAl 10 O 17 :Eu 2+ with 365 nm UV chip as excitation. The correlation color temperature (CCT) value and the chromaticity coordinates of the fabricated WLED devices were found to be 5522 K and (0.3293, 0.3036) respectively. In conclusion, Ba 2 CaB 2 Si 4 O 14 :Eu 2+ has great potential in the preparation of near UV excited white LEDs.
研究领域:
材料科学
·
光电技术
·
光子学
✓ 已验证
DOI
高影响因子
MACROMOLECULAR BIOSCIENCE
·
2022
·Vol. 22
·期 7
·2200105
·
IF 5.859
Pengqi Wan,Wei Guo,Yuxiu Duan,Mingxiao Deng,Chunsheng Xiao
Recently, continuous emergence of resistant bacteria has appeared as one of the most serious threats to human health. Therefore, systematic exploration of new antibacterial materials is of guiding significance. In this study, a series of photosensitizer-polypeptide conjugate (PPa-cP) is readily synthesized through simple ring-opening reactions to realize the synergistic antibacterial effects on Staphylococcus aureus ( S. aureus ) and methicillin-resistant S. aureus (MRSA) under light irradiation. Compared with free PPa, the cationic PPa-cP shows enhanced binding ability with the negative surface of S. aureus through electrostatic interaction, exhibiting effective antibacterial activity against both S. aureus and MRSA in vitro under light irradiation. Among the synthesized PPa-cP, PPa-cP5 with the degree of polymerization of 37 and modified with a 1-methylimidazole side group exhibits the best antibacterial activity with a minimum inhibitory concentration value of 2 µ m without light irradiation and 0.25 µ m with light irradiation. Moreover, PPa-cP5 shows good hemocompatibility. The above-mentioned results elucidate that the positively charged PPa-cP5 can significantly increase the efficiency of photodynamic therapy and effectively eradicate S. aureus biofilm due to its potent penetration ability into S. aureus biofilms. Overall, the present study establishes an efficient strategy for the treatment of S. aureus and S. aureus biofilm infections.
研究领域:
微生物学
·
药理学
·
生物材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF PHARMACEUTICS
·
2022
·
IF 6.51
Liang-Liang Chen,Wen-Pu Shi,Tuo-Di Zhang,Ya-Qing Zhou,Feng-Zhu Zhao,Wan-Yi Ge,Xiao-Qian Jin,Wen-Juan Lin,Wei-Hong Guo,Da-Chuan Yin
Staphylococcus aureus ( S. aureus ) infection, especially its drug-resistant bacterial infection, is a great challenge often faced by clinicians and patients, and it is also one of the most important threats to public health. Finding a safe and effective antibacterial agent is of great significance for the prevention and treatment of S. aureus infection. Lysozyme is known to have antibacterial effects against Gram-positive bacteria including S. aureus . Here, high-quality lysozyme with a purity of more than 99% and an activity of more than 60, 000 U/mg was prepared from egg white, which showed excellent antibacterial activity against three strains of S. aureus , especially against MRSA . Furthermore, an antibacterial cream loaded with lysozyme was prepared and tested in scald wound healing. The lysozyme-loaded cream exhibited the effect of preventing wound infection and promoting wound healing on scalds, and no toxicity was found in animal organs. Overall, lysozyme showed great application potential in the prevention and treatment of infections caused by S. aureus and scalded wound healing. The most remarkable discovery in this work is the unexpectedly powerful inhibitory effect of lysozyme on the drug-resistant bacterial, especially MRSA , which is usually very difficult to deal with using normal antibacterial drugs.
研究领域:
药学
·
微生物学
·
伤口愈合
·
传染病学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
COLORATION TECHNOLOGY
·
2019
·Vol. 135
·期 4
·267-274
·
IF 1.241
Biaobiao Yan,Muying Yang,Qingqing Zhou,Tieling Xing,Guoqiang Chen,Jiayong Sheng
Microbial dyes have received substantial attention because of their natural environmental protection, simple access, and reduced regional and seasonal restriction. In this work, a microbial dye, the yellow pigment produced by Metarhizium anisopliae , was first studied then applied. The strain produced by the culture was identified, and the conditions for producing yellow pigment were optimised. Further, the stability of M. anisopliae yellow pigment was examined, and the pigment was applied to the dyeing of silk and wool fabrics. The results showed that the homology of the strain with M. anisopliae was 99.98%. In liquid fermentation culture, the optimal carbon source was glucose, and the dosage was 30 g/l. The maximum pigment yield can be obtained by culturing with 4% v/v of inoculation quantity at pH 7 and 30 °C. In addition, the effects of pH, temperature and metal ions on the yellow pigment of M. anisopliae were significant. The optimum dyeing process conditions were dyeing temperatures of 80 °C for silk and 90 °C for wool, with a dyeing time of 60 min. This research developed a novel microbial dye and studied its application for the dyeing of protein fibres.
研究领域:
微生物学
·
纺织科学
·
环境科学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF ELECTRONIC MATERIALS
·
2018
·Vol. 47
·期 12
·7497-7504
·
IF 1.566
Zheng Jingxia,Wang Junli,Wang Yaling,Yang Yongzhen,Liu Xuguang,Xu Bingshe
A one-pot microwave-assisted hydrothermal approach was developed to quickly synthesize yellow fluorescent carbon dots (CDs) using o -phenylenediamine and urea as raw materials. The optimized reaction temperature of 140°C and reaction time of 10 min were adopted to synthesize the CDs with photoluminescence quantum yield of 4.23%. The as-prepared CDs exhibit yellow fluorescence ( λ em ∼ 563 nm) and an excitation-independent PL emission in the region of 440–700 nm. The CDs were combined with a blue chip ( λ ex = 420 nm) to fabricate white light-emitting diodes (WLEDs) and realize white light emission. The WLED has the color coordinates of (0.30, 0.30) and the correlated color temperature of 7915 K, which is suitable for office and outdoor illumination. The results demonstrate that as-obtained CDs can be a promising candidate as yellow-emitting phosphors for WLEDs.
研究领域:
材料科学
·
光学工程
✓ 已验证
产品关联
DOI
高影响因子
Materials Today Communications
·
2025
·
IF 4.5
Yan Li,Hongyang Yan,Yuanhui Li,Xin Huang,Jingwen Wang,Chenxiang Tang,Cheng Luo
The emergence of antibiotic resistance represents a significant and growing threat to global public health. The development of novel and antibiotic-free antibacterial strategies is therefore highly importance. In this context, chemodynamic therapy (CDT), which is based on Fenton or Fenton-like chemistry, is a promising strategy. CDT utilizes Fe 2+ to convert H 2 O 2 into highly reactive hydroxyl radicals (•OH), which can oxidize biomolecules to achieve antibacterial effects. The antibacterial efficacy of CDT is contingent upon both the catalyst and substrate. We developed a straightforward, convenient and economical approach to fabricate Fe-caffeic acid metal–polyphenol networks (Fe-CA MPNs) by mixing CA and Fe 3+ . The Fe-CA MPNs are acid-sensitive and self-enhanced CDT agents with remarkable antibacterial efficacy against both Staphylococcus aureus ( S. aureus ) and methicillin-resistant S. aureus (MRSA). Antibacterial efficacy was evaluated via a variety of methods, including the zone of inhibition, spread plate, turbidimetric, and live/dead bacterial staining tests. Specifically, the inhibition rates of Fe-CA MPNs for S. aureus and MRSA were 96.33 ± 0.33 % and 96.33 ± 0.15 %, respectively. CaO 2 @Fe-CA nanocomposites with H 2 O 2 self-supply properties were synthesized by depositing Fe-CA MPNs on the surface of CaO 2 nanoparticles which function as a sustained-release source of H 2 O 2 . The antibacterial effect of the CaO 2 @Fe-CA nanocomposites was comparable to that of Fe-CA MPNs combined with H 2 O 2 . Both Fe-CA MPNs and CaO 2 @Fe-CA possess favorable in vitro biosafety profiles. This work will facilitate the development of innovative antibiotic-free CDT antibacterial strategies based on •OH.
研究领域:
微生物学
·
材料科学
·
医学
✓ 已验证
产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Jilei Wang,Qu Li,Yun Sun,Jingwen Liu,Zhao Jiang,Zhuoping Zheng,Li Liu,Xinhui Min,Yougui Yu,Qing Zheng
Elucidating the mechanisms underlying Baijiu production is a shared aspiration among academic groups specializing in the field of Baijiu research. This study comprehensively examined the mechanisms underlying the yellowish coloration of Baijiu through a synergistic application of chromatographic, spectroscopic, and physical methodologies. Aging of Baijiu in earthenware pots involves the infiltration of mineral ions such as iron, aluminum, and calcium; however, these ions are detected at extremely low concentrations and are therefore not linked to the development of Baijiu's yellowish color. Instead, the yellowish coloration is attributed to the diverse colorants generated during the high-temperature fermentation of small-molecule sugars derived from the saccharification of grain materials. Although these colorants exist in minimal quantities and exhibit spectral absorption peaks ranging from 300 to 450 nm, their overlapping spectra collectively contribute to the light-absorbing properties of Baijiu across a broad wavelength range, ultimately accounting for its characteristic yellowish color.
研究领域:
食品科学
·
化学
✓ 已验证
DOI
PubMed
高影响因子
FOOD CONTROL
·
2018
·
IF 3.667
Jin-Rong Bai,Kai Zhong,Yan-Ping Wu,Grosu Elena,Hong Gao
The aim of this study was to evaluate the inhibitory activity of shikimic acid (SA) against biofilm formation of Staphylococcus aureus and to explore its potential molecular mechanism. Crystal violet staining assay showed that SA clearly reduced the biomass of the biofilm at its sub-MICs. Light microscopic images and scanning electron microscope (SEM) observations suggested that SA inhibited the biofilm adhesion on glass slides and induced apparent collapse of biofilm architecture. In addition, XTT reduction assay and confocal laser scanning microscopic images illustrated that the metabolic activity and the viability of the SA-treated biofilm cells significantly decreased. Cell mobility of the SA-treated S. aureus was also inhibited. These observations suggested that SA inhibited the initial biofilm formation by interfering the metabolic activity, the cell viability, and the cell mobility of S. aureus . However, SA failed to disrupt the one-day-old mature biofilm of S. aureus even at the concentrations higher than MIC, suggesting that SA disturbed the early steps in the biofilm formation of S. aureus . qRT-PCR analyses showed that SA down-regulated the transcription of sarA , while up-regulated the transcription of agrA , which resulted in the prevention of biofilm formation. So, SA could be a potential antibiofilm agent to inhibit biofilm formation of S. aureus .
研究领域:
微生物学
·
药理学
·
分子生物学
✓ 已验证
DOI
高影响因子
Journal of Materials Chemistry C
·
2019
·Vol. 8
·期 3
·1143-1150
·
IF 6.641
Zhizhen Wang,Zhen Song,Lixin Ning,Quanlin Liu
Warm-color persistent luminescent materials are strongly desired for signage markings and medical imaging in comparison with green or blue counterparts. Herein we report a novel yellow long-persistent phosphor, Nb-doped Sr3SiO5:Eu2+, with a peak wavelength of ∼580 nm and persistence time of more than 14 hours at the 0.32 mcd m−2 threshold value after UV radiation. A combination of thermoluminescence (TL), thermoluminescence excitation (TLE), electron paramagnetic resonance (EPR) measurements and density functional theory (DFT) calculations reveals that the persistent luminescence enhancement is attributed to a significant Nb-induced increase of oxygen vacancies that act as electron trapping centers with appropriate trap depths. Groups of time-dependent color-change images are realized with this material, which has potential applications as anti-counterfeit and indicator marks. This investigation also expands the application of transition metal (TM) ions to the field of persistent luminescence and would motivate further exploration of TM substitutions to design and improve silicate or aluminosilicate persistent phosphors with superior performance.
研究领域:
材料科学
·
光学
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）