Surfaces and Interfaces
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2026
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IF 6
Xueying Zhang,Wei Lai,Mengdi Guo
Quality assessment of herbal medicines is complicated by their complex matrices and variability in chemical composition. In this study, a three-dimensional surface-enhanced Raman scattering (SERS) substrate was constructed for the analysis of Astragalus membranaceus extracts. The substrate consists of carboxylated carbon nanotubes (HOOC CNTs) integrated with Ti 3 C 2 T x MXene and decorated with in situ-grown silver nanoparticles (AgNPs), forming an interconnected porous network. This hierarchical architecture provides accessible plasmonic sites, while the CNT–MXene framework improves material dispersion and limits MXene restacking in aqueous media. The AgNP/HOOC CNT/Ti 3 C 2 T x substrate exhibited a measured SERS enhancement factor of 1.82 × 10 6 , together with improved signal uniformity and stability under the tested conditions. Quantitative calibration models were established for astragalosides, polysaccharides, and calycosin in A. membranaceus extracts with limited sample pretreatment, and the analytical results were compared with those obtained using reference methods. The measured chemical profiles also enabled preliminary discrimination among the investigated samples from different geographical origins and assessment of batch-to-batch variation. Among the samples included in this study, those collected from Jilin showed relatively high levels of the target constituents. These results indicate that the proposed SERS substrate has potential as a rapid analytical platform for compositional assessment and comparative quality evaluation of A. membranaceus . Further validation using larger and more geographically representative sample sets is required before the method can be applied to routine origin authentication and quality grading. Download: Download high-res image (370KB) Download: Download full-size image
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Journal of Inflammation Research
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2025
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IF 4.1
Jing Ma,Lu Ding,Xiaoyu Zang,Yingying Yang,Wei Zhang,Xiangyan Li,Daqing Zhao,Zepeng Zhang,Zeyu Wang,Linhua Zhao,Xiaolin Tong
Purpose Pulmonary Fibrosis (PF) is a severe interstitial lung disease currently lacking effective prevention strategies. Endothelial mesenchymal transition (EndMT), a novel mechanism for fibroblast production, is closely associated with PF. The precise mechanisms underlying the contribution of EndMT-derived fibroblasts to PF, however, remain unclear.Methods Using network pharmacology, molecular docking, and molecular dynamics, we identified the key targets and pathways through which Qimai Feiluoping decoction (QM) combats PF. EndMT and autophagy proteins were quantified in bleomycin (BLM) -induced C57BL/6 mice, human umbilical vein endothelial cells (HUVECs), and zebrafish using Western blotting (WB), quantitative real-time polymerase chain reaction (qRT-PCR), immunohistochemistry (IHC), immunofluorescence (IF), Transwell migration assays, and transmission electron microscopy (TEM), revealing the targets and pathways through which QM mitigates PF.Results Network pharmacology, molecular docking, and molecular dynamics suggest that QM combats PF by modulating the PI3K/AKT/mTOR pathway. Observations from the study indicated that QM was found to alleviate EndMT by restoring autophagy, primarily through inhibition of the PI3K/AKT/mTOR signaling pathway in both BLM-induced C57 mice and HUVECs. Supporting evidence from zebrafish models demonstrated that QM not only counteracts EndMT but also improves a range of vascular functional disorders and remodeling issues following EndMT.Conclusion Our research validates the active compounds, core targets, and signaling pathways through which QM counters PF, providing valuable insights for its therapeutic application in PF management.
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EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS
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2025
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IF 4.3
Yue Zhao,Yinlian Yao,Shilong Fan,Xin Shen,Jiahao Liu,Xingxing Chai,Xueqin Huang,Hua Jin,Zhikun Zhou
Acute lung injury (ALI) and acute respiratory distress syndrome (ARDS) frequently occur alongside sepsis, presenting significant challenges and associated mortality rates between 25 % and 40 %. Despite notable advancements in medical treatment, effective pharmacological options for ALI/ARDS remain limited due to rapid systemic clearance and insufficient targeting of lung tissues. To address this issue, we developed nanoparticles loaded with Astragaloside IV (ASIV-NPs) using an emulsification-evaporation method. Network pharmacology revealed 72 shared targets between ASIV and acute pneumonia, with core nodes (AKT1, CASP3, BCL2, IL6) identified through protein interaction analysis. Enrichment studies linked these targets to critical pathways including JAK-STAT signaling and cellular stress response. Molecular docking confirmed ASIV’s strong binding affinity (≤-5 kcal/mol) to key proteins governing inflammation and apoptosis. In vitro, PM@ASIV-NPs demonstrated biocompatibility, targeted inflamed macrophages, suppressed pro-inflammatory cytokines (IL-6, TNF-α, IL-1β), and scavenged ROS. In vivo imaging showed precise lung accumulation, while intranasal administration in ALI mice significantly enhanced IL-10, reduced inflammatory markers, and improved survival. The combined pharmacological evidence elucidates ASIV’s multimodal mechanism through target-pathway modulation, aligning with its observed therapeutic effects. This biomimetic nanoplatform utilizing platelet membrane camouflage offers a promising strategy for targeted ALI/ARDS treatment, with potential applicability to COVID-19-related pneumonia.
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BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
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2023
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IF 3.1
Chan-juan Chai,Yao Sun,Rui-fang Chi,Hui-yu Yang,Bin Yang,Bao Li
Cardiac remodeling (CR), characterized by cardiac hypertrophy and fibrosis, leads to the development and progression of heart failure (HF). Nowadays, emerging evidence implicated that inflammation plays a vital role in the pathogenesis of CR and HF. Astragaloside IV (AS-IV), an effective component of Astragalus membranaceus , exerts cardio-protective and anti-inflammatory effects, but the underlying mechanism remains not fully elucidated. This present study aimed to investigate the effects of AS-IV on cardiac hypertrophy and fibrosis in cultured H9C2 cells stimulated with LPS, as well as explore its underlying mechanisms. As a result, we found AS-IV could reduce the cell surface size, ameliorate cardiac hypertrophy and fibrosis in LPS-induced H9C2 cells. To specify which molecules or signaling pathways play key roles in the process, RNA-seq analysis was performed. After analyzing the transcriptome data, CCL2 has captured our attention, of which expression was sharply increased in model group and reversed by AS-IV treatment. The results also indicated that AS-IV could ameliorate the inflammatory response by down-regulating NF-κB signaling pathway. Additionally, a classical inhibitor of CCL2 (bindarit) were used to further explore whether the anti-inflammatory effect of AS-IV was dependent on this chemokine . Our results indicated that AS-IV could exert a potent inhibitory effect on CCL2 expression and down-regulated NF-κB signaling pathway in a CCL2-dependent manner. These findings provided a scientific basis for promoting the treatment of HF with AS-IV.
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Natural Product Communications
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2023
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IF 1.8
Qing Xu,Dekun Li,Meixu Wan,Yanxin Zhang,Zhi Li,Aichun Ju
ObjectiveIn this study, an online CMC–LC/MS method was established in order to screen the active components of Yiqi Fumai lyophilization injection (YQFM) related to angiogenesis. The pro-angiogenic active components of YQFM were screened using this system and were then further verified.MethodsThe ECV304/CMC model coupled online with an HPLC–MS system was initially developed to screen, analyze, and identify the allergenic components of YQFM. A fraction was retained on the ECV304/CMC column, which was identified as ginsenoside Rb2. A cell proliferation assay and zebrafish intestinal microvascular model labeled with green fluorescence were used to detect the proliferation effect of ginsenoside Rb2.Results(1) The active component in YQFM, which was retained on the column, was ginsenoside Rb2. (2) Within a concentration range of 2.5–20 μg/mL, ginsenoside Rb2 was shown to promote the proliferation of ECV304 and EPCs cells and increase the area of sub-intestinal vessels in zebrafish.ConclusionGinsenoside Rb2, the active component in YQFM, can promote angiogenesis. The ECV304/CMC-online-HPLC system may serve as an effective screening tool for the vasoactive components from the traditional Chinese medicine injection.
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Bioactive Materials
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2022
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IF 16.874
Tianshu Chu,Qingye Li,Chun Dai,Xiang Li,Xiang Kong,Yangming Fan,Hongyan Yin,Jianjun Ge
Vein grafts are widely used for coronary artery bypass grafting and hemodialysis access, but restenosis remains the "Achilles' heel" of these treatments. An extravascular stent is one wrapped around the vein graft and provides mechanical strength; it can buffer high arterial pressure and secondary vascular dilation of the vein to prevent restenosis. In this study, we developed a novel Nanocellulose-gelatin hydrogel, loaded with the drug Astragaloside IV (AS-IV) as an extravascular scaffold to investigate its ability to reduce restenosis. We found that the excellent physical and chemical properties of the drug AS-IV loaded Nanocellulose-gelatin hydrogel external stent limit graft vein expansion and make the stent biocompatible. We also found it can prevent restenosis by resisting endothelial-to-mesenchymal transition (EndMT) in vitro . It does so by activating autophagy, and AS-IV can enhance this effect both in vivo and in vitro . This study has added to existing research on the mechanism of extravascular stents in preventing restenosis of grafted veins. Furthermore, we have developed a novel extravascular stent for the prevention and treatment of restenosis. This will help optimize the clinical treatment plan of external stents and improve the prognosis in patients with vein grafts.
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Oxidative Medicine and Cellular Longevity
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2022
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IF 7.31
Xiaoyu Zhu,Fangze Tao,Li Zhang,Yajie Yu,Yanchao Xu,Gang Yang,Zhifeng Wei,Yidong Cheng,Xuelai Yin,Xinyuan Zhang,Wu Wei,Anxi Wang
Aims. Bladder outlet obstruction (BOO) and the consequent low contractility of detrusor are the leading causes of voiding dysfunction. In this study, we aimed to evaluate the pharmacological activity of astragaloside IV (AS-IV), an antioxidant biomolecule that possess beneficial effect in many organs, on detrusor contractility and bladder wall remodeling process. Methods. Partial BOO (pBOO) was created by urethral occlusion in female rats, followed by oral gavage of different dose of AS-IV or vehicle. Cystometric evaluation and contractility test were performed. Bladder wall sections were used in morphology staining, and bladder tissue lysate was used for ELISA assay. Primary smooth muscle cells (SMCs) derived from detrusor were used for mechanism studies. Results. Seven weeks after pBOO, the bladder compensatory enlarged, and the contractility in response to electrical or chemical stimuli was reduced, while AS-IV treatment reversed this effect dose-dependently. AS-IV also showed beneficial effect on reversing the bladder wall remodeling process, as well as reducing ROS level. In mechanism study, AS-IV activated mitophagy and alleviated oxidative stress via an AMPK-dependent pathway. Conclusion. Out data suggested that AS-IV enhanced the contractility of detrusor and protected the bladder from obstruction induced damage, via enhancing the mitophagy and restoring mitochondria function trough an AMPK-dependent way.
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ACS Nano
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2023
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IF 17.1
Xin Zhao,Jinlong Luo,Ying Huang,Lei Mu,Jueying Chen,Zhen Liang,Zhanhai Yin,Dake Chu,Yong Han,Baolin Guo
Developing injectable antiswelling and high-strength bioactive hydrogels with wet tissue adhesiveness and a rapid gelling process to meet the requirements for rapid hemostasis, sutureless wound closure, and scar-free repair of infected skin wounds continues to have ongoing challenges. Herein, injectable, antibacterial, and antioxidant hydrogel adhesives based on poly(citric acid-co-polyethylene glycol)-g-dopamine and amino-terminated Pluronic F127 (APF) micelles loaded with astragaloside IV (AS) are prepared. The H2O2/horseradish peroxidase (HRP) system is used to cause cross-linking of the hydrogel network through oxidative coupling between catechol groups and chemical cross-linking between the catechol group and the amino group. The hydrogels exhibit a rapid gelling process, high mechanical strength, an antiswelling effect, good antioxidant property, H2O2 release behavior, and degradability. In addition, the hydrogels present good wet tissue adhesiveness, high bursting pressure, excellent antibacterial activity, long-term sustained release of AS, and good biocompatibility. The hydrogels perform good hemostasis on mouse liver, rat liver, and rabbit femoral vein bleeding models and achieve much better closure and healing of skin incisions than biomedical glue and surgical sutures. Furthermore, the hydrogel dressing significantly improved the scar-free repair of MRSA-infected full thickness skin defect wounds by modulating inflammation, regulating the ratio of collagen I/III, and improving the vascularization and granulation tissue formation. Thus, AS-loaded hydrogels show huge potential as multifunctional dressings for in vivo hemostasis, sutureless wound closure, and scar-free repair of infected skin wounds.
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JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
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2020
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IF 2.734
Xiaowei Wang,Yufen Li,Ji Li,Yaxuan Gu,Lihong Sang,Dongkai Wang
The combination of multiple therapies could significantly improve the efficiency of treatment for malignant tumors, which has become the trend of cancer research and treatment. Astragaloside IV (AS-IV) is able to enhance the sensitivity of anti-tumor drugs and reduces the adverse reactions, but AS-IV alone is not much powerful lethality to cancer cells. AS-IV and photosensitizer ICG are combined, which contributes to elevate the anti-tumor effect. In this study, we constructed the liposome co-loading with AS-IV and near infrared (NIR) dye ICG for chemo-photothermal therapy of breast cancer. In addition, the anticancer properties of the liposome have been studied in vitro and in vivo . AS-IV/ICG-LPs showed the uniform structure, preferable photothermal response, excellent physiological stability, and photo-responsive drug release behavior. Furthermore, in comparison with individual chemotherapy or PTT, the combinational chemo-PTT therapy of AS-IV/ICG-LPs with NIR laser irradiation synergistically induced apoptosis and death of cancer cells in vitro , and demonstrated synergistic chemo-PTT efficiency in vivo as evidenced by highly efficient tumor tissue ablation. Moreover, the liposome materials of FDA-approved agents provide a safe strategy in the rational design of synergistic combination therapy.
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Biomed Research International
·
2019
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IF 2.197
Dongyu Xie,Ping Zhou,Lin Liu,Wenjing Jiang,Haina Xie,Liang Zhang,Donghao Xie
Suitable content of iron is essential for human body, but iron overload is associated with many kinds of diseases including chronic liver damage. Recently, researchers find that iron overload promotes hepatocyte autophagy and apoptosis. However, the mechanism of iron overload in liver damage remains unclear. In this study, Lo2 cells were selected as the research object, iron dextran was a model drug, and astragaloside IV was a therapeutic drug to explore the role of iron overload. MTT assay and Annexin/PI double staining were used to measure cell viability and apoptosis. Ultrastructure was observed by transmission electron microscopy. The expression levels of apoptosis and autophagy-related proteins were determined by real-time PCR and Western Blot. The results showed that iron dextran could significantly inhibit Lo2 cell viability and increase the apoptosis rate, while astragaloside IV could reverse the inhibition of Lo2 cell viability and decrease the apoptosis rate. Transmission electron microscopy showed a significant increase in the number of autophagosomes after administration of iron dextran, and the application of astragaloside IV reduced the production of autophagosomes. LC3II/I was significantly upregulated in the model group but decreased in the astragaloside IV treatment group, and P62 showed the opposite trend. Iron dextran significantly upregulated the expression of Bax and downregulated Bcl2, while astragaloside IV reversed this trend. Finally, the inhibition of hepcidin caused by iron dextran was counteracted by astragaloside IV. In conclusion, the experimental results show that the iron overload model mainly induces excessive autophagy and apoptosis of hepatocytes, thus causing damage to hepatocytes, but astragaloside IV plays a certain therapeutic role in reversing this damage.
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EUROPEAN JOURNAL OF PHARMACOLOGY
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2019
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IF 3.17
Jiamao Lin,Lijun Fang,Hui Li,Zhenxiang Li,Linmao Lyu,Haiyong Wang,Jun Xiao
Doxorubicin (DOX) is a classic anti-tumor chemotherapeutic used to treat a wide range of tumors. One major downfall of DOX treatment is it can induce fatal cardiotoxicity. Astragaloside IV (AS-IV) is one of the primary active ingredients that can be isolated from the traditional Chinese herbal medicine, Astragalus membranaceus . This study uses both in vitro and in vivo tools to investigate whether AS-IV alleviates DOX induced cardiomyopathy . We found that AS-IV supplementation alleviates body weight loss, myocardial injury , apoptosis of cardiomyocytes, cardiac fibrosis and cardiac dysfunction in DOX-treated mice. Also, DOX-induced cardiomyocyte injury and apoptosis were effectively improved by AS-IV treatment in vitro . NADPH oxidase (NOX) plays an important role in the progress of the oxidative signal transduction and DOX-induced cardiomyopathy. In this study, we found that AS-IV treatment relieves DOX-induced NOX2 and NOX4 expression and oxidative stress in cardiomyocytes. In conclusion, AS-IV, an antioxidant, attenuates DOX-induced cardiomyopathy through the suppression of NOX2 and NOX4.
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Chinese Medicine
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2024
·Vol. 19
·期 1
·1-17
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IF 5.3
Gao Jiaming,Guo Hao,Li Junmei,Zhan Min,You Yue,Xin Gaojie,Liu Zixin,Fan Xiaodi,Gao Qinghe,Liu Jianxun,Zhang Yehao,Fu Jianhua
Background Buyang Huanwu Decoction (BYHWD) is a traditional Chinese medicine to treat the syndrome of qi deficiency and blood stasis. Platelets play an important role in regulating thrombus and inflammation after ischemic injury, studies have shown that BYHWD regulate myocardial fibrosis and exert anti-inflammatory effects through IL-17 and TLR4 pathways, but the mechanism of platelet activation by BYHWD in stable coronary heart disease is still unknown. In the present study, model of left anterior descending coronary artery ligation was applied to investigate the mechanisms of BYHWD on modulating platelets hyperreactivity and heart function after fibrosis of ischemic myocardial infarction (MI). Methods Myocardial infarction model was constructed by ligation of the left anterior descending coronary artery. The rats were randomly divided into five groups: sham, model, MI with aspirin (positive), MI with a low dosage of BYHWD (BYHWD-ld) and MI with a high dosage of BYHWD (BYHWD-hd) for 28 days. Results Coronary artery ligation prominently induced left ventricle dysfunction, increased cardiomyocyte fibrosis, which was accompanied by platelets with hyperreactivity, and high levels of inflammatory factors. BYHWD obviously reversed cardiac dysfunction and fibrosis, increased the thickness of the left ventricular wall, and inhibited aggregation ratio and CD62p expression. BYHWD restored the mitochondrial respiration of platelets after MI, concomitant with an increased telomere expression and decreased inflammation. According to the result of transcriptome sequencing, we found that 106 differentially expressed genes compared model with BYHWD treatment. Enrichment analysis screened out the Ras-related protein Rap-1 (Rap1) signaling pathway and platelet activation biological function. Quantitative real-time PCR and Western blotting were applied to found that BYHWD reduced the expression of Rap1/PI3K-Akt/Src-CDC42 genes and attenuated the overactivity of PI3 kinase/Rap1/integrin α(IIb)β(3) pathway. Conclusion BYHWD reduced inflammation and platelet activation via the PI3 kinase/Rap1/integrin α(IIb)β(3) pathway and improved heart function after MI. Graphical Abstract
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