Xiao Wang,Qian Tan,Xiyue Bao,Xinyue Gong,Lingmin Zhao,Jieyin Chen,Lei Liu,Ran Li
Tomatoes are important horticultural crops worldwide. Verticillium wilt is a disease caused byVerticillium dahliaethat causes serious tomato yield losses.V. dahliaecan be classified into three distinct races in tomatoes. We identified the specificVdR3egene ofV. dahliaerace 3 and found that VdR3e triggered immune responses in the resistant tomato cultivar IVF6384. We confirmed that VdR3e triggers immune responses in the parents of IVF6384 plants and conducted transcriptome sequencing between male and female IVF6384 plants after VdR3e infiltration to analyze the potential regulatory network response to VdR3e. We found that both parents had a series of detoxification and stress resistance responses to VdR3e, but those of the male IVF6384 parent were concentrated in disease resistance-related signaling pathways. Moreover, several vital differentially expressed genes involved in functional annotation related to plant–pathogen interactions and plant hormone signaling stimulated immune responses inNicotiana benthamiana. This study provides a new and comprehensive perspective on tomato resistance to Verticillium wilt.
Patatin-like proteins (PLPs) have non-specific lipid acyl hydrolysis (LAH) activity, which can hydrolyze membrane lipids into fatty acids and lysophospholipids. The vital role of PLPs in plant growth and abiotic stress has been well documented. However, the function of PLPs in plant defense responses against pathogens is still poorly understood. Here, we isolated and identified a novel cotton (Gossypium hirsutum) PLP gene GhPLP2. The expression of GhPLP2 was induced upon treatment with Verticillium dahliae, the signaling molecules jasmonic acid (JA) and ethylene (ETH) in cotton plants. Subcellular localization revealed that GhPLP2 was localized to the plasma membrane. GhPLP2-silenced cotton plants were more susceptible to infection by V. dahliae, while the overexpression of GhPLP2 in Arabidopsis enhanced its resistance to V. dahliae, which was apparent as mild symptoms, and a decrease in the disease index and fungal biomass. The hypersensitive response, deposition of callose, and H2O2 accumulation triggered by V. dahliae elicitor were reduced in GhPLP2-silenced cotton plants. The overexpression of GhPLP2 in Arabidopsis resulted in the accumulation of linoleic acid (LA, 18:2) and α-linolenic acid (ALA, 18:3) and facilitated the biosynthesis of JA and JA-mediated defensive responses. GhPLP2 silencing in cotton plants consistently reduced the accumulation of linoleic acid (LA, 18:2) and α-linolenic acid (ALA, 18:3) and suppressed the biosynthesis of JA and the defensive responses mediated by JA. These results indicate that GhPLP2 is involved in the resistance of cotton to V. dahliae by maintaining fatty acid metabolism pools for JA biosynthesis and activating the JA signaling pathway.
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