PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY
·
2020
·
IF 1.646
Yan Jin Gao,Wei Jie Fu,Jia Liu,Yi Juan Chen,Guang Hui Dai
Cucumber powdery mildew (PM) is a destructive fungal disease which has a substantial impact on cucumber yield and quality. Our previous work has demonstrated that (+)-( S )-ar-turmerone (ATM) is an effective compound for controlling PM infected by Podosphaera xanthii in cucumber. In this study, the effect of ATM on cucumber PM was histologically investigated by observations on the morphology of the pathogenic fungus at different time points after inoculation. It was found that the ATM caused the collapse of the pathogen's conidia and mycelia structure, the cytoplasm coagulation, the reduction of the conidia germination rate, and the inhibition of the conidiophore growth, therefore, prevented the re-infection of the P. xanthii . Evaluation of the content of total phenols and flavonoids in cucumber ATM-treated leaves showed that the ATM-treatment could increase the content of total phenols and flavonoids in cucumber leaves significantly in comparison to the control. A further analysis of the contents of the compounds showed that ATM-treatment could increase the content of rutin , quercetin , hesperetin , and gallic acid , syringic acid , vanillic acid and salicylic acid. Meanwhile, the activity of phenylalanine ammonia-lyase (PAL) and polyphenol oxidase (PPO) in cucumber leaves were increased slightly, but the activity of peroxidase (POD) and superoxide dismutase (SOD) were significantly increased through ATM treatment. It should be estimated from this study that the ATM not only directly affected the growth of P. xanthii but also enhanced the cucumber plant resistance to the disease.
研究领域:
植物病理学
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植物化学
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产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
·
2014
·Vol. 49
·期 10
·2290-2298
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IF 1.354
Yurong Ma,Huihua Huang
Summary To investigate the relationship between bioactive compounds and antioxidant property, total phenolic content (TPC), total flavonoid content (TFC), individual and total isoflavone content of soymilk and their correlations with oxygen-radical-absorbing capacity (ORAC), ferric reducing antioxidant power (FRAP) and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging capacity values were evaluated and compared. Results showed that TPC, TFC, isoflavones (including concentration and profiles) and antioxidation activity significantly varied among ten tested soybean cultivars. Significant correlations were established between DPPH and TFC ( r = 0.553, P < 0.01), DPPH and TPC ( r = 0.753, P < 0.01), FRAP and TFC ( r = 0.599, P < 0.01) and FRAP and TPC ( r = 0.616, P < 0.01). Positive linear correlations were found between subtotal, total isoflavones and ORAC. Unlike other isoflavone monomers, aglycone isoflavones correlated positively with DPPH and FRAP significantly. DPPH well correlated with FRAP, whereas neither DPPH nor FRAP correlated with ORAC. TPC, TFC, individual and total isoflavone values are potentially useful for soymilk antioxidant activity assessment.
研究领域:
食品科学
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营养学
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DOI
高影响因子
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2024
·Vol. 25
·期 2
·709
·
IF 4.9
Seungmee Lee,Hyeonmi Shin,Jihyun Bae,Taek Lee,Minji Kim,Heung Bae Jeon,Kang Hyun Lee,Hah Young Yoo,Chulhwan Park
Puerarin is a flavonoid known as a natural antioxidant found in the root ofPueraria robata. Its antioxidant, anticancer, and anti-inflammatory effects have attracted attention as a potential functional ingredient in various bioindustries. However, puerarin has limited bioavailability owing to its low lipid solubility and stability. Acylation is proposed as a synthesis method to overcome this limitation. In this study, lipase-catalyzed acylation of puerarin and various acyl donors was performed, and the enzymatic synthetic condition was optimized. Under the condition (20 g/L of Novozym 435, palmitic anhydride, 1:15, 40 °C, tetrahydrofuran (THF)), the synthesis of puerarin ester achieved a significantly high conversion (98.97%) within a short time (3 h). The molecule of the synthesized puerarin palmitate was identified by various analyses such as liquid chromatography–mass spectrometry (LC–MS), Fourier-transform infrared spectroscopy (FT-IR), and carbon-13 nuclear magnetic resonance (13C NMR). The lipid solubility and the radical scavenging activity were also evaluated. Puerarin palmitate showed a slight decrease in antioxidant activity, but lipid solubility was significantly improved, improving bioavailability. The high conversion achieved for puerarin esters in this study will provide the foundation for industrial applications.Keywords:antioxidant;flavonoid;puerarin;flavonoid ester;lipase;lipid solubility
研究领域:
生物化学
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制药工程
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DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2022
·
IF 5.304
Guifang Qin,Jie Lei,Sihui Li,Yongmei Jiang,Lei Qiao,Mengdie Ren,Qiong Gao,Changwei Song,Shaobin Fu,Jinquan Zhou,Shiji Xiao,Maosheng Zhang,Gang Wang
In this study, ultrasonic-assisted deep eutectic solvents (DESs-UAE) was applied for efficient and environmentally friendly extraction of amentoflavone (AME) and robustaflavone (ROB) from Selaginella uncinata. Firstly, proline-lactic acid (Pro-Lac) was screened as the optimal DES from the 30 DESs. The maximum extraction rates of AME and ROB were obtained adopting the response surface methodology (RSM). Under the optimal conditions of molar ratio of 1:4, water content of 33 %, and extraction time of 30 min, the extraction yields of AME and ROB were 709.72 ± 11.31 μg/g and 145.13 ± 9.77 μg/g, respectively. Compared with the traditional extraction methods, the DESs-UAE extraction could enhance the extraction rates and save extraction time. At the same time, 23 flavonoids were identified by UPLC-Q-TOF-MS, of which 6 components appeared for the first time in S. uncinata. In addition, three antioxidant experiments (DPPH method, ABTS method, and hydroxyl radical scavenging method) were carried out, and the results showed that the S. uncinata extracts had potential antioxidant activity. Furthermore, S. uncinata extracts exhibited obvious growth inhibitory effect on A549, HCT-116, HT-29, sw1990, HepG2 and HeLa cancer cells. In conclusion, deep eutectic solvent, as an promising solvent, is much more effective and simple extraction of bioactive constituents from S. uncinata with high safety and low toxicity.
研究领域:
药物化学
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分析化学
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植物科学
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生物技术
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肿瘤学
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产品关联
DOI
高影响因子
JOURNAL OF ETHNOPHARMACOLOGY
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2013
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IF 2.755
Linling Lu,Dawei Qian,Jianming Guo,Yefei Qian,Boyi Xu,Mei Sha,Jinao Duan
Aim Abelmoschi Corolla is a well-known herbal medicine used for the treatment of chronic renal disease . Flavonoids are the major bioactive ingredients of Abelmoschi Corolla, but some non-flavonoid components also exist in this herb. In order to clarify the influences of non-flavonoid components on the pharmacokinetics profile of the flavonoid fraction from Abelmoschi Corolla (FFA), an investigation was carried out to compare the pharmacokinetic parameters of seven flavonoid components after administration of FFA and after administration of FFA combined with different non-flavonoid fractions. Materials and methods A selective and sensitive UPLC–MS/MS method was established to determine the plasma concentrations of the seven compounds. Sprague-Dawley rats were allocated to four groups which orally administered FFA, FFA combined with macromolecular fraction (FFA–MF), FFA combined with small molecule fraction (FFA–SF) and FFA combined with MF–SF (FFA–MF–SF) with approximately the same dose of FFA. At different time points, the concentration of rutin (1), hyperoside (2), isoquercitrin (3), hibifolin (4), myricetin (5), quercetin-3′- O -glucose (6), quercetin (7) in rat plasma were determined and main pharmacokinetic parameters including T 1/2 , T max , AUC and C max were calculated using the DAS 2.0 software package. The statistical analysis was performed using the Student's t -test with P <0.05 as the level of significance. Results Flavonoids almost had similar pharmacokinetics profile that were rapidly absorbed, reached the peak concentration at 30–60 min in group A, but the pharmacokinetic profiles and parameters of these flavonoids changed when co-administered with non-flavonoid components. It was found that AUC of five flavonoids but not hibifolin and quercetin in group FFA–SF and group FFA–MF–SF increased ( P <0.05) in comparison with group FFA while the tendency was not observed in group FFA–MF. Moreover, seven flavonoids had varying degrees of differences in the pharmacokinetics parameters such as C max , T max and T 1/2 ( P <0.05) in group FFA–MF, FFA–SF and FFA–MF–SF by comparison with group FFA. Conclusion These results indicate that non-flavonoid components could improve the bioavailability and delay the elimination of some flavonoids in rat.
研究领域:
中药学
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药理学
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生物化学
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DOI
PubMed
高影响因子
AMERICAN JOURNAL OF CHINESE MEDICINE
·
2024
·Vol. 52
·期 04
·1137-1154
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IF 4.8
Dan-Dan Yang,Shu-Yi Li,Xiao-Wei Xu,Qing-Yao Li,Jia-Yuan He,Lian-Di Zhou,Qi-Hui Zhang
Flavonol and flavonoid compounds are important natural compounds with various biomedical activities. Therefore, it is of great significance to develop a strategy for the specific extraction of flavonol and flavonoid compounds. Quercetin is a well-studied flavonoid possessing many health benefits. This compound is a versatile antioxidant known to possess protective abilities against body tissue injury induced by pathological situations and various drug toxicities. Although quercetin is widely distributed in many plants, its content generally is not very high. Therefore, the specific extraction of quercetin as well as other flavonol and flavonoid compounds has profound significance. In this work, the quercetin molecularly imprinting polymer (QMIP) was successfully prepared, in which a typical flavonol quercetin was selected as the template molecule. QMIP was synthesized by performing the surface molecular imprinting technology on the surface of NH 2 -MIL-101(Fe). Our study results showed that QMIP exhibited quick binding kinetic behavior, a high adsorption capacity (57.04 mg/g), and the specific recognition ability toward quercetin compared with structurally distinct compounds (selective factor > 2 ). The specific adsorption ability of quercetin by QMIP was further explained using computation simulation that molecules with non-planar 3D conformations hardly entered the molecularly imprinted cavities on QMIP. Finally, QMIP was successfully used for the specific extraction of quercetin and five other flavonol and flavonoid compounds in the crude extracts from Sapium sebiferum . This study proposes a new strategy to synthesize the molecularly imprinted polymer based on a single template for enriching and loading a certain class of active ingredients with similar core structures from variable botanicals.
研究领域:
分析化学
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天然产物化学
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产品关联
DOI
PubMed
高影响因子
PEST MANAGEMENT SCIENCE
·
2023
·Vol. 79
·期 10
·3570-3580
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IF 4.1
Weiting Jiao,Ping Zhang,Chuanjian Cui,Min Yan,Qing X. Li,Yongfeng Tang,Nan Zhang,Xinyi Wang,Ruyan Hou,Rimao Hua
Background Thiamethoxam (TMX) is insecticidal, but also can trigger physiological and metabolic reactions of plant cycles. The objective of this work was to evaluate the physiological and metabolic effect of TMX on tea plants and its potential benefits. Results In this study, dose of TMX (0.09, 0.135 and 0.18 kg a.i./ha) were tested. Except for peroxidase (POD) and glutathione S-transferase (GST), chlorophyll, carotenoid, catalase (CAT) and malondialdehyde (MDA) were significantly affected compared with the controls. The CAT activity was increased by 3.38, 1.71, 2.91 times, respectively, under three doses of TMX treatment. The metabolic response between TMX treatment and control groups on the third day was compared using a widely targeted metabolomics. A total of 97 different metabolites were identified, including benzenoids, flavonoids, lipids and lipid-like molecules, organic acids and derivatives, organic nitrogen compounds, organic oxygen compounds, organoheterocyclic compounds, phenylpropanoids and polyketides, and others. Those metabolites were mapped on the perturbed metabolic pathways. The results demonstrated that the most perturbation occurred in flavone and flavonol biosynthesis. The beneficial secondary metabolites luteolin and kaempferol were upregulated 1.46 and 1.31 times respectively, which protect plants from biotic and abiotic stresses. Molecular docking models suggest interactions between TMX and flavonoid 3-O-glucosyltransferase. Conclusion Thiamethoxam spray positively promoted the physiological and metabolic response of tea plants. And this work also provided the useful information of TMX metabolism in tea plants as well as rational application of insecticides. © 2023 Society of Chemical Industry.
研究领域:
农业科学
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植物生理学
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代谢组学
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DOI
PubMed
高影响因子
Frontiers in Bioengineering and Biotechnology
·
2021
·
IF 5.89
Wang Zhaoyu,Li Yang,Li Mingyi,Zhang Xiaohui,Ji Qingxia,Zhao Xiaojuan,Bi Yanhong,Luo Si
The conversion of flavonoid glycosides and their analogs to their lipophilic ester derivatives was developed by nanobiocatalysts from immobilizing Thermomyces lanuginosus lipase (TLL) on polydopamine-functionalized magnetic Fe3O4 nanoparticles (Fe3O4-PDA-TLL). The behavior investigation revealed that Fe3O4-PDA-TLL exhibits a preference for long chain length fatty acids (i.e., C10 to C14) with higher reaction rates of 12.6–13.9 mM/h. Regarding the substrate specificity, Fe3O4-PDA-TLL showed good substrate spectrum and favorably functionalized the primary OH groups, suggesting that the steric hindrances impeded the secondary or phenolic hydroxyl groups of substrates into the bonding site of the active region of TLL to afford the product.
研究领域:
生物化学
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酶工程
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有机化学
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DOI
PubMed
高影响因子
RSC Advances
·
2021
·Vol. 11
·期 54
·34343-34354
·
IF 3.361
Liling Wang,Yuchuan Qin,Yanbin Wang,Yifeng Zhou,Bentong Liu,Minge Bai,Xiaoqing Tong,Ru Fang,Xubo Huang
The inhibition mechanism of two homoisoflavonoids from Ophiopogon japonicus including methylophiopogonanone A (MO-A) and methylophiopogonanone B (MO-B) on tyrosinase (Tyr) was studied by multiple spectroscopic techniques and molecular docking. The results showed that the two homoisoflavonoids both inhibited Tyr activity via a reversible mixed-inhibition, with a half inhibitory concentration (IC50) of (10.87 ± 0.25) × 10−5 and (18.76 ± 0.14) × 10−5 mol L−1, respectively. The fluorescence quenching and secondary structure change of Tyr caused by MO-A and B are mainly driven by hydrophobic interaction and hydrogen bonding. Molecular docking analysis indicated that phenylmalandioxin in MO-A and methoxy in MO-B could coordinate with a Cu ion in the active center of Tyr, and interacted with amino acid Glu322 to form hydrogen bonding, occupying the catalytic center to block the entry of the substrate and consequently inhibit Tyr activity. This study may provide new perspectives on the inhibition mechanism of MO-A and MO-B on Tyr and serve a scientific basis for screening effective Tyr inhibitors.
研究领域:
生物化学
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药理学
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DOI
PubMed
高影响因子
MOLECULES
·
2020
·Vol. 25
·期 4
·858
·
IF 3.267
Xiaojuan Liu,Yue Wang,Yezhi Chen,Shuting Xu,Qin Gong,Chenning Zhao,Jinping Cao,Chongde Sun
O-methylation of flavonoids is an important modification reaction that occurs in plants.O-methylation contributes to the structural diversity of flavonoids, which have several biological and pharmacological functions. In this study, anO-methyltransferase gene (CrOMT2) was isolated from the fruit peel ofCitrus reticulata, which encoding a multifunctionalO-methyltransferase and could effectively catalyze the methylation of 3’-, 5’-, and 7-OH of flavonoids with vicinal hydroxyl substitutions. Substrate preference assays indicated that this recombinant enzyme favored polymethoxylated flavones (PMF)-type substrates in vitro, thereby providing biochemical evidence for the potential role of the enzyme in plants. Additionally, the cytotoxicity of the methylated products from the enzymatic catalytic reaction was evaluated in vitro using human gastric cell lines SGC-7901 and BGC-823. The results showed that the in vitro cytotoxicity of the flavonoids with the unsaturated C2-C3 bond was increased after being methylated at position 3’. These combined results provide biochemical insight regarding CrOMT2 in vitro and indicate the in vitro cytotoxicity of the products methylated by its catalytic reaction.Keywords:citrus;flavonoids;O-methyltransferase;bioactivity;cytotoxicity
研究领域:
植物生物化学
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药理学
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分子生物学
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细胞生物学
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DOI
PubMed
高影响因子
FOOD RESEARCH INTERNATIONAL
·
2025
·
IF 8
Zhanming Tang,Yanyan Miao,Huimin Lu,Chao Chen,Shiming Li,Wensheng Zhang
Citri Reticulatae Pericarpium (Xinhui Chenpi) , a representative product with a protected geographical indication from Xinhui District, Guangdong Province, China, is a dual-use medicinal and edible material and a traditional medicinal resource whose quality is traditionally believed to improve with prolonged aging. Increasing evidence indicates that the succession of surface microbial communities and their metabolic activities play pivotal roles in these transformations. This study systematically investigated Xinhui Chenpi aged 1–10 years to elucidate the dynamics of dominant fungal genera and experimentally verify their involvement in flavonoid transformation. Ultra-performance liquid chromatography (UPLC), third-generation full-length ITS sequencing (PacBio) , and strain isolation with culture assays were employed to characterize flavonoid profiles, fungal community succession, and the transformation capabilities of key isolates. Flavonoids exhibited a “degradation to resynthesis” trajectory, with polymethoxyflavones (PMFs) steadily accumulating throughout the aging process. Late-stage samples were dominated by the xerophilic genus Xeromyces (Aspergillaceae), which showed strong positive correlations with PMF enrichment, whereas correlations with Penicillium progressively declined over time. Importantly, Aspergillus tubingensis was isolated and confirmed to convert nobiletin into 3′-demethylnobiletin, providing direct mechanistic evidence for fungal-driven PMF structural modification. Community profiling further revealed that multiple Aspergillaceae genera ( Aspergillus , Penicillium , Xeromyces ) may participate in flavonoid transformation. These findings elucidate the microbial mechanisms underlying quality enhancement in aged Chenpi and provide a scientific basis for microbial-assisted optimization of modern aging processes, thereby offering theoretical support for the traditional principle that “aged Chenpi becomes more efficacious.”
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PubMed
高影响因子
RSC Advances
·
2021
·Vol. 11
·期 23
·13769-13779
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IF 3.361
Yao Lu,Yi Xu,Meng-Ting Song,Ling-Ling Qian,Xiao-Lin Liu,Rong-Yao Gao,Rui-Min Han,Leif H. Skibsted,Jian-Ping Zhang
Tyrosinase, widely distributed in nature, is a copper-containing polyphenol oxidase involved in the formation of melanin. Flavonoids are most often considered as tyrosinase inhibitors but have also been confirmed to be tyrosinase substrates. Four structure-related flavonoids including flavones (apigenin and luteolin) and flavonols (kaempferol and quercetin) are found to promote not inhibit browning induced by tyrosinase catalyzed oxidation both in model systems and in mushrooms under aerobic conditions. A comparison with enzymatic oxidation and autooxidation of flavonoids alone has helped to clarify why flavonoids function as a substrate rather than an inhibitor. Flavonoids almost do not affect the kinetics of melanin formation from enzymatic oxidation of L-dopa in excess. In addition, a new brown complex formed during the reaction of flavonoid quinone and dopaquinone is suggested to enhance the browning effects by competing with isomerization and autooxidation. Structure–activity relationships of the four flavonoids in melanin formation leading to browning induced by autooxidation and enzymatic oxidation confirm the enzymatic nature of the browning.
研究领域:
生物化学
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食品科学
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药理学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）