Polymer Chemistry
·
2017
·Vol. 8
·期 35
·5344-5353
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IF 5.375
Min He,Qian Wang,Weifeng Zhao,Jianshu Li,Changsheng Zhao
We report on a self-defensive bilayer hydrogel coating which can switch from a cell adhesion surface to an antibacterial adhesion surface. To covalently attach the coating, an antifouling hydrogel thin film was firstly prepared on a thiol modified substrate as the bottom layer via the thiol–ene click reaction of the ene-functionalized copolymer of poly(sulfobetaine methacrylate-acrylate acid-2-hydroxyethyl methacrylate) (P(SBMA-AA-HEMA)). Thereafter, heparin-mimicking polymer chains were grafted onto the hydrogel film surface as the upper layer via surface initiated atom transfer radical polymerization. The ester group of HEMA was designed as the sensitive part which could be cleaved in a bacterial infectious environment, while the hydroxyl group was designed as the anchoring point of the upper layer. The surface morphology and chemical composition after each modification step were characterized by scanning electron microscopy, atomic force microscopy, and X-ray photoelectron spectroscopy. The bilayer coating could promote cell adhesion and proliferation, as well as bacterial adhesion. After being immersed into a cholesterol esterase solution (simulative bacterial infectious environment), the upper layer could be detached rapidly, and the exposed bottom layer then showed strong resistance to bacteria. Our approach towards bacteria-responsive “intelligent” coating gives new insights into the antibacterial protection of biomedical devices.
研究领域:
生物材料学
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医学工程
·
表面科学
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DOI
高影响因子
Materials Science & Engineering C-Materials for Biological Applications
·
2019
·
IF 4.959
Kunyu Zhang,Zi Wei,Xiayi Xu,Qian Feng,Jianbin Xu,Liming Bian
Hydrogels are promising soft materials for the delivery of therapeutic cells and cargo molecules. Inspired by mussel adhesion chemistry, hydrogels based on catechol (Cat)-modified polysaccharides have been developed to enhance hydrogel–tissue interactions. However, due to the inevitable side reactions such as self-polymerization of dopamine involved in the conventional catechol conjugation process, the efficiency of catechol conjugation to polymers is typically low, leading to insufficient stability, low mechanical strength, and poor adhesiveness of these catechol-modified hydrogels. In this study, we report a new approach to synthesize catechol-functionalized hyaluronic acid with improved degree of substitution. Due to the significantly increased conjugated Cat groups, the obtained HA-Cat hydrogels not only can adhere to tissue samples under wet conditions but also can capture cell adhesion proteins to enhance cell attachment and spreading. Meanwhile, owing to the extensive Cat–protein interactions, these hydrogels can also facilitate long-term release of protein-based therapeutic cargoes, such as the osteoinductive BMP-2 protein, thereby effectively promoting osteogenic differentiation of stem cells. These findings show that the HA-Cat hydrogels are ideal carriers of therapeutic cells and drugs for tissue regeneration.
研究领域:
材料科学
·
生物医学工程
·
组织工程
·
药学
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DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Rui Xue,Juan Wang,Weiwei Huang,Yibin Qiu,Hong Xu,Rui Wang,Sha Li
Mussel foot proteins (mfps) play important roles in surface interaction and underwater adhesion. However, limited production and the lack of adhesion of recombinant mfps have restricted their widespread use. Here, we present a general strategy for enhancing both the expression and function of mfps by connecting multiple protein fragments. We demonstrate that fp-535, the fusion protein comprising fp-5 at each fp-3 terminus, exhibits best adhesion strength up to 1.43 MPa and coating ability, while achieving a higher yield of 91.13 mg/L in the flask culture. After optimizing the fermentation parameters such as temperature and volume as well as the culture medium, the yield of fp-535 was increased to 1.70 g/L in a 5-L bioreactor. Our approach highlights the advantage of fusion mfps and provides potential for the sustainable supply of practical bioadhesive materials.
研究领域:
生物化学
·
海洋生物学
·
生物技术
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DOI
PubMed
高影响因子
LANGMUIR
·
2014
·
IF 4.384
Xuetao Shi,Serge Ostrovidov,Yiwei Shu,Xiaobin Liang,Ken Nakajima,Hongkai Wu,Ali Khademhosseini
Engineered surface-bound molecular gradients are of great importance for a range of biological applications. In this paper, we fabricated a polydopamine gradient on a hydrophobic surface. A microfluidic device was used to generate a covalently conjugated gradient of polydopamine (PDA), which changed the wettabilty and the surface energy of the substrate. The gradient was subsequently used to enable the spatial deposition of adhesive proteins on the surface. When seeded with human adipose mesenchymal stem cells, the PDA-graded surface induced a gradient of cell adhesion and spreading. The PDA gradient developed in this study is a promising tool for controlling cellular behavior and may be useful in various biological applications.
研究领域:
材料科学
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生物材料
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细胞生物学
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组织工程
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DOI
PubMed
高影响因子
MRS Communications
·
2025
·
IF 2.3
Tan Jing Er,Liu Shengnan,Bai Jun,Yu Bingbing,Chen Kuntao,Sheng Xing,Yin Lan
Wet-adhesive hydrogels hold significant promise for biomedical applications, yet achieving long-term adhesion remains a major challenge in biodegradable systems. Here, we present a biodegradable hydrogel incorporating a water-absorbent layer to regulate swelling behavior and water content. Comprising a poly(ethylene glycol) diacrylate (PEGDA)–gelatin matrix with a starch-based absorbent layer, the hydrogel exhibits high wet adhesion energy, elevated burst pressure, effective in vivo hemostasis, and mechanical compatibility with soft tissue. This water-absorbent strategy provides a new avenue for achieving sustained and stable wet tissue adhesion in biodegradable hydrogel systems. Graphical abstract
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INTERNATIONAL JOURNAL OF PHARMACEUTICS
·
2017
·
IF 3.649
Wenxi Wang,Anna Shao,Shisen Feng,Min Ding,Gan Luo
S-layer proteins (Slps) are crystalline arrays of protein on bacterial cell surface layers. Owning to their capability to reassemble on the surface of lipid layers, Slps have been employed to modify liposomes for various profits. But the interaction information between Slps and positively charged liposomes are destitute, especially the gastrointestinal adhesion of Slps-coating liposomes is rarely reported. In the present work, the Slps extracted from Lactobacillus helveticus were reassembled on the surface of novel positively charged liposomes composed of soybean lecithin , Eudragit ® RL100 and cholesterol. The particle size and remarkable changes of Zeta potential with various Slps/lipid weight ratios were determined by dynamic light scattering and phase analysis light scattering. Significant difference in fluorescence dequenching percentage of liposomes decorated by ODA-FITC confirmed Slps self-reassemble on the surface of liposomes. A higher integrity of vesicular membrane after the addition of Triton X-100 solution demonstrated the stability enhancement of Slps-coating liposomes. Fourier transform infrared (FTIR) spectroscopy illustrated the interaction came from non-covalent bond. The mucoadhesion of Slps-coating liposomes was evaluated by the resident FITC-LP on the gastric and intestinal tract of mice at 7 h and 12 h after intragastrical administration, which proved that the Slps-coating improved the gastrointestinal adhesion significantly.
研究领域:
药剂学
·
生物物理学
·
材料科学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2023
·
IF 9.9
Yuhan Guo,Minglong Yan,Wenjie Zhao
Marine biofouling caused a number of questions about energy consumption and safety. While there were still some challenges in developing an environmentally friendly, non-toxic and long-term antifouling slippery liquid-infused porous surface (SLIPS). Here, we proposed a difunctional antifouling strategy via constructing porous polydimethylsiloxane (PDMS) surface with a layer of aerogel by sol-gel method and grafted cinnamaldehyde chemically. The improvement in structure enhanced the liquid storage stability of coating, which in turn increases its anti-bioadhesive ability. In addition, the grafted cinnamaldehyde could also be used to act as a chemical antibacterial and is intelligently released in the face of harsh fouling environments, which played a key role in prolonging the antibacterial lifespan of the coating. After the 120-hour anti-bacteria experiment and the 25-day anti-algae experiment, the anti- Escherichia coli (anti- E. coli ) rate and the anti-algae rate of the coating reached 99.6% and 99.9%, respectively, which was attributed to the excellent long-term antifouling properties of the coating. The combination of physical and chemical antifouling property made the coating achieve long-term fouling prevention for marine engineering equipment.
研究领域:
材料科学
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海洋生物学
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环境科学
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DOI
PubMed
高影响因子
ADVANCED FUNCTIONAL MATERIALS
·
2021
·Vol. 31
·期 14
·2007457
·
IF 18.808
Lei Zhou,Cong Dai,Lei Fan,Yuhe Jiang,Can Liu,Zhengnan Zhou,Pengfei Guan,Yu Tian,Jun Xing,Xiaojun Li,Yian Luo,Peng Yu,Chengyun Ning,Guoxin Tan
Biocompatible hydrogel adhesives with multifunctional properties, including injectability, fast self-healing, and suitable on-demand detachment, are highly desired for minimally invasive procedures, but such materials are still lacking. Herein, an injectable self-healing biocompatible hydrogel adhesive with thermoresponsive reversible adhesion based on two extracellular matrix-derived biopolymers, gelatin and chondroitin sulfate, is developed to be used as a surgical adhesive for sealing or reconnecting ruptured tissues. The resulting hydrogels present good self-healing and can be conveniently injected through needles. The strong tissue adhesion at physiological temperatures originates from the Schiff base and hydrogen bonding interactions between the hydrogel and tissue that can be weakened at low temperatures, thereby easily detaching the hydrogel from the tissue in the gelation state. In vivo and ex vivo rat model show that the adhesives can effectively seal bleeding wounds and fluid leakages in the absence of sutures or staples. Specifically, a proof of concept experiment in a damaged rat liver model demonstrates the ability of the adhesives to act as a suitable laparoscopic sealant for laparoscopic surgery. Overall, the adhesive has several advantages, including low cost and ease of production and application that make it an exceptional multifunctional tissue adhesive/sealant, effective in minimally invasive surgical applications.
研究领域:
生物医学工程
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材料科学
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外科技术
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组织工程
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产品关联
DOI
高影响因子
Microorganisms
·
2021
·Vol. 9
·期 7
·1511
·
IF 4.128
Jia Yin,Yujie Song,Yaozhong Hu,Yuanyifei Wang,Bowei Zhang,Jin Wang,Xuemeng Ji,Shuo Wang
Akkermansia muciniphila, a potential probiotic, has been proven to lessen the effects of several diseases. As established, the relative abundance ofAkkermansiais positively correlated with tryptophan metabolism. However, the reciprocal interaction between tryptophan andAkkemansiais still unclear. Herein, for the first time, the possible effects of tryptophan and its derived metabolites onA. muciniphilawere preliminarily investigated, including growth, physiological function, and metabolism. Obtained results suggested that 0.4 g/L of tryptophan treatment could significantly promote the growth ofA. muciniphila. Notably, when grown in BHI with 0.8 g/L of tryptophan, the hydrophobicity and adhesion ofA. muciniphilawere significantly improved, potentially due to the increase in the rate of cell division. Furthermore,A. muciniphilametabolized tryptophan to indole, indole-3-acetic acid, indole-3-carboxaldehyde, and indole-3-lactic acid. Indoles produced by gut microbiota could significantly promote the growth ofA. muciniphila. These results could provide a valuable reference for future research on the relationship between tryptophan metabolism andA. muciniphila.Keywords:Akkermansia;tryptophan metabolites;indole;indole derivates
研究领域:
微生物学
·
营养学
·
胃肠病学
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DOI
PubMed
高影响因子
RSC Advances
·
2019
·Vol. 9
·期 70
·40887-40894
·
IF 3.049
Xue Yang,Ke Ma,Libo Yang,Yujuan Chen,Yingmin Qu,Ying Wang,Xinyue Wang,Fan Yang,Qi Sun,Zhengxun Song,Zuobin Wang
This paper employs a spin-coated method to construct conductive polypyrrole (PPy) substrates which present superior properties for controlling the morphological structures and functions of bEnd.3 cells. The PPy substrates with a homogeneous particle size, uniform distribution and proper roughness show enhanced hydrophilic characteristics and improve cell adhesion to the substrates. The changes in the mechanical properties of cells and the responses to the designed substrates and magnetic field are also explored. Due to the synergistic effect between the magnetic field and the conductive PPy substrate, the cells cultured in such an environment exhibit applanate shapes with more branches and enhanced cell viability. In addition, the cells preferentially extend along the magnetic field direction. The mechanical characteristics of cells change significantly under varying magnetic intensity stimulations (5–16 mT). The satisfying effect on cells' morphology and outgrowth is acquired at the magnetic intensities of 9–10 mT and duration of 20 min, compared with other stimulated groups, while retaining cell viability. Moreover, the cells express higher adhesion up to 5.2 nN. The results suggest that the application of the PPy substrates and magnetic field is a promising candidate for the protection of neurovascular units and treatment of neurological diseases.
研究领域:
生物医学工程
·
神经科学
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产品关联
DOI
PubMed
高影响因子
BIOMACROMOLECULES
·
2023
·
IF 6.2
Tao Wang,Haowei Fang,Subate Yalikun,Jinyan Li,Yuqing Pan,Kunxi Zhang,Jingbo Yin,Haiyan Cui
Developing strong anti-inflammatory wound dressings is of great significance for protecting inflammatory cutaneous wounds and promoting wound healing. The present study develops a nanocomposite Pluronic F127 (F127)-based hydrogel dressing with injectable, tissue adhesive, and anti-inflammatory performance. Briefly, Ce3+/tannic acid/ulinastatin nanoparticles (Ce3+/TA/UTI NPs) are fabricated. Meanwhile, α-lipoic acid is bonded to the ends of F127 to prepare F127-lipoic acid (F127LA) and its nanomicelles. Due to the gradual viscosity change instead of mutation during phase transition, the mixed Ce3+/TA/UTI NPs and F127LA nanomicelles show well-performed injectability at 37 °C and can form a semisolid composite nanohydrogel that can tightly attach to the skin at 37 °C. Furthermore, ultraviolet (UV) irradiation without a photoinitiator transforms the semisolid hydrogel into a solid hydrogel with well-performed elasticity and toughness. The UV-cured composite nanohydrogel acts as a bioadhesive that can firmly adhere to tissues. Due to the limited swelling property, the hydrogel can firmly adhere to tissues in a wet environment, which can seal wounds and provide a reliable physical barrier for the wounds. Ce3+/TA/UTI NPs in the hydrogel exhibit lipopolysaccharide (LPS)-scavenging ability and reactive oxygen species (ROS)-scavenging ability and significantly reduce the expression of inflammatory factors in wounds at the early stage, accelerating LPS-induced wound healing.
研究领域:
材料科学
·
医学
·
药学
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产品关联
DOI
PubMed
高影响因子
Journal of Materials Chemistry B
·
2024
·
IF 6.1
Shengjun Cheng,Haifeng Ji,Tao Xu,Xianda Liu,Lin Xu,Weifeng Zhao,Changsheng Zhao
Heparin coatings are widely applied on blood-contact materials to reduce the use of anticoagulants during blood treatment. However, the previous heparin coatings formed via covalent binding or electrostatic bonding commonly require complex surface premodification, and the blood coagulation pathway was significantly inhibited to potentially increase the bleeding risk. This contradicts the intended purpose and deviates from the anticoagulation mechanism of the heparin coatings. Herein, we present a facile and substrate-independent coating, achieved through the co-deposition of dopamine/chitosan followed by electrostatic interaction between heparin and the immobilized chitosan, which could be prepared within 1 hour. This coating prolonged the plasma re-calcification time (PRT) to over 60 minutes, effectively preventing surface-induced thrombosis. Favorable hemocompatibility was reflected in a hemolysis ratio of less than 2%, low levels of platelet adhesion and activation, and low levels of fibrinogen adhesion. We also systematically elucidate the anticoagulant mechanism of the coating, demonstrating why the coating can prevent thrombogenesis without the bleeding risk. Our work not only offers a promising and readily available heparin coating for blood-contact materials, but more importantly, the mechanism exploration supports the practical feasibility of heparin coating in various applications.
研究领域:
材料科学
·
生物材料
·
生物医学工程
·
止血
·
血栓
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）