MOLECULES
·
2017
·Vol. 22
·期 11
·1914
·
IF 2.861
Yong Wang,Jie Zhang,Yang Sun,Juntao Feng,Xing Zhang
Fusarium wilt and anthracnose are two major diseases which limit the yield and quality of cucumber worldwide. Cuminic acid was extracted from the seed ofCuminum cyminumL. The mean EC50values of cuminic acid for inhibiting mycelial growth and zoospore germination of fiveFusarium oxysporumf. sp.cucumerinumstrains were 25.66 ± 3.02 μg/mL and 15.99 ± 2.19 μg/mL, and of fourColletotrichum lagenarium(Pass.) Ellis and Halsted strains were 29.53 ± 3.18 μg/mL and 18.41 ± 2.78 μg/mL, respectively. In greenhouse experiments, cuminic acid at 2000 μg/mL exhibited 70.77% protective and 62.63% curative efficacies againstF. oxysporum, and 65.43% protective and 55.46% curative efficacies againstC. lagenarium. Moreover, the translocation behavior of cuminic acid, determined by high performance liquid chromatography (HPLC), showed that it could be readily absorbed and transported upward and downward in cucumber. Importantly, superoxide dismutase (SOD) and pyphenol oxidase (PPO) activities of cucumber leaves treated with cuminic acid increased significantly. All results indicated that cuminic acid showed antifungal activity, and could be used as a botanical fungicide in disease management. This study encourages further investigation on the mechanism of action of cuminic acid and the development of alternative antifungal drugs.Keywords:botanical fungicide;cuminic acid;cucumber;F. oxysporum;C. lagenarium;control efficacy
研究领域:
植物保护
·
分子生物学
✓ 已验证
DOI
PubMed
高影响因子
PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY
·
2025
·
IF 3.3
Shuang Min,Jiaxin Liu,Canying Li,Tian Gao,Yajun Wang,Yonghong Ge
Black spot rot, caused by the pathogen Alternaria alternata , is a prevalent disease in apple cultivation. The present study investigated the effects of phenyllactic acid (PLA) immersion on black spot rot in apples and elucidated the potential mechanisms underlying its action. Results revealed that 1.00 g L -1 PLA inhibited the lesion development in A. alternata- inoculated apples. PLA resulted in a decrease in malondialdehyde (MDA) content, while simultaneously increasing the levels of H 2 O 2 , ascorbic acid (AsA), and reduced glutathione (GSH) in apples. PLA also induced the increase in gene expressions and enzymatic activities of superoxide dismutase (SOD), catalase (CAT), monodehydroascorbate reductase (MDHAR), dehydroascorbic acid reductase (DHAR), ascorbate peroxidase (APX), glutathione reductase (GR), peroxidase (POD), polyphenol oxidase (PPO), cinnamate 4-hydroxylase (C4H), 4-coumarate/coenzyme A ligase (4CL), and phenylalanine ammonia lyase (PAL) in apples. Moreover, inoculation of both PLA-treated and control fruit with A. alternata led to a further increase in the levels of H 2 O 2 , GSH, and AsA, along with enhanced gene expressions and enzymatic activities of CAT, SOD, DHAR, GR, APX , MDHAR, POD, PPO, C4H, 4CL and PAL, compared to un-inoculated fruit. Additionally, PLA dipping increased total phenols and flavonoids contents in apples, and inoculation with A. alternata further augmented the accumulation of these bioactive compounds. Furthermore, PLA-treated fruit displayed a more rapid response to A. alternata inoculation compared to control fruit, characterized by heightened enzymatic activity, gene expression, and metabolite content. Collectively, PLA has the potential to stimulate disease resistance against A. alternata in apples through regulating major enzyme activities, gene expressions, and metabolites contents involved in reactive oxygen species and phenylpropane metabolisms.
研究领域:
植物病理学
·
分子生物学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）