Frontiers in Chemistry
·
2023
·
IF 5.5
Liping Zhao,Ye Zhao,Lihe Zhao,Gang Liu
A nitrogen–sulfur dual-doped black fungus porous carbon (NS-FPC) matrix was prepared with natural black fungus as the carbon source and cysteine as the nitrogen–sulfur source. A black fungus-based solution was obtained by hydrothermal treatment. After further carbonization activation and combination with sulfur processing, the NS-FPC/S positive electrode materials were prepared. The uniform recombination of biomass carbon provides an efficient conductive framework for sulfur. The porous structure is conducive to the transport of electrolytes. Heteroatom doping can provide a more active site. The structure and composition analyses of the materials were carried out using X-ray diffraction (XRD). The electronic binding energy and bonding state were analyzed by X-ray photoelectron spectroscopy (XPS). The morphology was observed by scanning electron microscopy and transmission electron microscopy. The specific surface area and pore size distribution were analyzed using an N 2 adsorption–desorption experiment. Sulfur loading was determined through thermogravimetric analysis. The electrochemical performance of NS-FPC/S in Li–S batteries was systematically investigated. The result shows that the NS-FPC/S electrode maintains more than 1,000 mAh g -1 reversible capacity after 100 cycles at 0.2 C current density, with a capacity retention of 85%. The cycle and rate performance are both considerably superior to those of traditional activated carbon materials.
研究领域:
材料科学
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能源存储
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产品关联
DOI
PubMed
高影响因子
ACS Omega
·
2023
·
IF 4.1
Zihan He,Haoying Song,Jingjing Cheng,Wenxiu Qiu,Junjie Hu,Zhenpeng Qiu,Qi Wang,Cong Chang,Guohua Zheng,Yan Meng
Selenium nanoparticles (Se NPs) have significant anticancer effects, but their poor water solubility and dispersibility limit their further applications in biomedical fields. Biomacromolecules have often been used as dispersants or stabilizers in synthesized Se NPs because they can enhance the dispersibility of Se NPs and reduce their side effects. Our previous studies reported a triple-helix β-glucan (BFP) from the fruiting bodies of black fungus, which showed a good self-assembly ability in constructing hollow nanotubes for loading metal nanoparticles. Therefore, in the present work, BFP nanotubes were designed as carriers to entrap large amounts of Se NPs in order to enhance their stability and anticancer effects. The results showed that Se NPs were successfully synthesized and loaded inside the BFP nanotubes, and the composite (BFP-Se) exhibited high stability and dispersibility due to the covalent Se–O bonds between the Se NPs and the hydroxyl groups on the BFP nanotubes. Moreover, BFP-Se showed significant effects on the proliferation, apoptosis, and cell cycle of HepG2 cells compared to those exhibited by Se NPs. The mechanism was associated with BFP, which acted as a dispersant or stabilizer, resulting in the enhanced cellular uptake of the Se NPs. BFP also activated the death receptor-mediated and mitochondria-mediated apoptotic pathways in HepG2 cells. These results suggest that BFP-Se has potential applications in biomedical fields, especially for the treatment of human liver cancers.
研究领域:
纳米技术
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生物医学工程
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癌症研究
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药学
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产品关联
DOI
PubMed
高影响因子
Journal of Future Foods
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2022
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IF 0
Yuetong Chen,Wenhao Yu,Shanshan Zhang,Yabin Niu,Yanrong Zhang,Tingting Liu
ABSTRACT In this paper, using to the comprehensive scoring method, pressure shear assisted extraction was used to prepare polysaccharide from Auricularia auricula (PS-AAP)by orthogonal test to optimize the extraction. The highest comprehensive score (90.70) was obtained under 1: 70 g/mL solid-liquid ratio, 110 °C reaction temperature, 60 min reaction time and 1.0 MPa reaction pressure. Compared to AAP by water bath(WB-AAP), PS-AAP led to a higher extraction efficiency and decreased the relative molecular weight. In addition, infrared spectrum(FT-IR) analysis showed that pressure shear assisted did not change the polysaccharide structure. Scanning electron microscope(SEM) showed that PS-AAP was smaller and more broken by pressure shear assisted. Furthermore, the ratio of glucose to mannose in PS-AAP was relatively higher than WB-AAP, which also proved that PS-AAP had certain antioxidant and hypoglycemic activities. Compared with WB-AAP, the inhibition α -glucosidase and α -amylase activity of PS-AAP increased by 11.37% and 24.43% respectively. These results revealed that PS-AAP has the poten- tial value of being a antioxidant and hypoglycemic agent.
研究领域:
食品科学
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生物化学
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DOI
JOURNAL OF APPLIED POLYMER SCIENCE
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2021
·Vol. 138
·期 48
·51537
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IF 3.125
Haoying Song,Na Hu,Ziwei Gao,Baohui Zhang,Junjie Hu,Zhenpeng Qiu,Guohua Zheng,Cong Chang,Yan Meng
Gold nanoparticles (AuNPs) with size of 12 ~ 25 nm were loaded on the dendritic nanotubes (DNTs), which were self-assembled by the triple-helix polysaccharides from black fungus (AF1). The characterization results proved that the AuNPs were dispersed on the surface of DNTs without affecting their tubular structure. Due to the dendritic structure, the loading content of AuNPs could arrived at 46.4%. Moreover, the potential anticancer activities of the complex (DNT-Au) were evaluated by the induction of apoptosis in HepG2 cells. It was found DNT-Au could be taken up by cells and enter into lysosomes, further inducing apoptosis in HepG2 cells by ROS-mediated mitochondrial dysfunction. This work was benefit for the development of natural polysaccharide as a substrate to stabilize nanoparticles in biomedical fields.
研究领域:
纳米技术
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癌症生物学
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生物医学工程
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药学
✓ 已验证
产品关联
DOI
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
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2025
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IF 5.4
Zhenxing Tan,Junxian Wu,Yuyin Lin,Wenxia Wang,Yong Yuan,Lihua Zhou
To effectively eliminate the widely detected naproxen (NPX) pollutant in the aqueous environment, it is essential to explore eco-friendly materials. Recently, biochar as an adsorbent material attracted considerable attention, owing to its unique ability to remove organic pollutants from water. Herein, Auricularia auricula biochar (a-BC-800) was fabricated through alkali treatment of Auricularia auricula biomass for activation, followed by carbonization at 800°C. Characterization via scanning electron microscopy and Brunauer-Emmett-Teller revealed that a-BC-800 possesses a very high specific surface area (SSA) of 1660 m²/g and a complex mesoporous structure properties. The combination of alkali activation and high-temperature carbonization was crucial for enhancing the SSA of the biochar. a-BC-800 exhibited a maximum adsorption capacity of approximately 300 mg/g, outperforming most NPX adsorbent materials reported in the literature. The NPX adsorption process closely aligns with the pseudo-second-order kinetic model and the Freundlich isotherm model. Analysis of the adsorption mechanism revealed that π-π interactions, pore filling, and van der Waals forces were the primary mechanisms responsible for the efficient adsorption of NPX by a-BC-800. This study demonstrates that the combination of high-temperature treatment and alkali activation is an effective strategy for enhancing the adsorption performance of Auricularia auricula biochar for NPX, offering significant potential for the efficient removal of environmental pollutants.
研究领域:
环境科学
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材料工程
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化学工程
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水污染控制
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吸附技术
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产品关联
DOI
高影响因子
Life-Basel
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2025
·Vol. 15
·期 8
·1236
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IF 3.4
Ming Zhao,Huiyan Huang,Bowen Li,Yu Pan,Chuankai Wang,Wanjia Du,Wenliang Wang,Yansheng Wang,Xue Mao,Xianghui Kong
With the acceleration of the pace of life, increased stress levels, and changes in lifestyle factors such as diet and exercise, the incidence of diseases such as cancer and immunodeficiency has been on the rise, which is closely associated with the impaired antioxidant capacity of the body. Polypeptides and polysaccharides derived from edible fungi demonstrate significant strong antioxidant activity and immunomodulatory effects.Auricularia auricula, the second most cultivated mushroom in China, is not only nutritionally rich but also offers considerable health benefits. In particular, its polysaccharides have been widely recognized for their immunomodulatory activities, while its abundant protein content holds great promise as a raw material for developing immunomodulatory peptides. To meet the demand for high-value utilization ofAuricularia auricularesources, this study developed a key technology for the stepwise extraction of polypeptides (AAPP1) and polysaccharides (AAPS3) using a composite enzymatic hydrolysis process. Their antioxidant and immunomodulatory effects were assessed using cyclophosphamide (CTX)-induced immune-suppressed mice. The results showed that both AAPP1 and AAPS3 significantly reversed CTX-induced decreases in thymus and spleen indices (p< 0.05); upregulated serum levels of cytokines (e.g., IL-4, TNF-α) and immunoglobulins (e.g., IgA, IgG); enhanced the activities of hepatic antioxidant enzymes SOD and CAT (p< 0.05); and reduced the content of MDA, a marker of oxidative damage. Intestinal microbiota analysis revealed that these compounds restored CTX-induced reductions in microbial α-diversity, increased the abundance of beneficial bacteria (Paramuribaculum,Prevotella;p< 0.05), decreased the proportion of pro-inflammatoryDuncaniella, and reshaped the balance of the Bacteroidota/Firmicutes phyla. This study represents the first instance of synergistic extraction of polypeptides and polysaccharides fromAuricularia auriculausing a single process. It demonstrates their immune-enhancing effects through multiple mechanisms, including “antioxidation-immune organ repair-intestinal microbiota regulation.” The findings offer a theoretical and technical foundation for the deep processing ofAuricularia auriculaand the development of functional foods.
研究领域:
免疫学
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药理学
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微生物学
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生物化学
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生物技术
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营养学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Surfaces and Interfaces
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2022
·
IF 6.137
Xiaoxuan Kang,Xigai Jia,Ziyang Kang,Yuchen Zhang,Dongbin Zhang,Jilun Wei,Aihong Guo,Ming Ge,Zhangxing He
N-doped biochar (N-BC) was prepared from black fungus by coupling a hydrothermal method with thermal pyrolysis , and the physicochemical properties of N-BC were characterized in detail by various techniques. As-synthesized N-BC exhibited a better catalytic performance to activate peroxydisulfate (PDS) for tetracycline (TC) degradation compared with as-obtained N-doped hydrochar (N-HC), N-doped calcined carbon (N CC) and most of the reported carbon catalysts. After reaction of 60 min, 89.8% of TC (20 mg/L) was degraded by N-BC/PDS oxidation system, and TC degradation obeyed the pseudo-second-order kinetic with a reaction rate constant at 0.843 L⋅mg −1 ⋅min −1 . The effect of some important parameters (N-BC dosage, PDS dose, initial TC concentration, solution pH, reaction temperature and inorganic anions) on TC degradation was also investigated and analyzed. The radical trapping experiments, EPR test, and electrochemical analysis confirmed that TC degradation in N-BC/PDS system was achieved via the radical (SO 4 •− , ‧ OH) and non-radical ( 1 O 2 , electron transfer) pathways, and the later played the dominant role. Finally, the performance of N-BC/PDS oxidation system for the removal of TC from the different real water matrices was studied. This study provided a new material for the preparation of highly active PDS catalyst for the treatment of antibiotic wastewater.
研究领域:
环境科学
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化学工程
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水处理
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催化
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材料科学
✓ 已验证
产品关联
DOI
高影响因子
Food Chemistry-X
·
2025
·
IF 8.2
Jiaying Zhu,Ting Zhang,Linhua Cui,Hong Liu,Qingyuan Wang,Yunlong Li,Qi Li
Solid-state fermentation of Tartary buckwheat using Auricularia auricula , Ganoderma lucidum , and Hericium erinaceus enhanced its functional components, increasing levels of phenols, polysaccharides, proteins, and amino acids, alongside improved antioxidant activity. The fermentation notably elevated flavonoid levels, particularly Quercetin-3-O-sulfate, which increased tenfold with Ganoderma lucidum . Analysis revealed 3078 metabolites, with downregulated metabolites predominantly being amino acids, carbohydrates, flavonoids, and phenolic compounds. Key metabolic pathways identified included amino sugar metabolism and biosynthesis of plant secondary metabolites, as per KEGG enrichment analysis. This research highlights solid-state fermentation as a promising method to enhance the functional properties of Tartary buckwheat, paving the way for developing functional foods.
研究领域:
食品科学
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生物技术
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微生物学
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营养学
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生物化学
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代谢组学
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功能性食品
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天然产物化学
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Shuangyu Shao,Xiali Si,Yingtao Zhang,Jun Li,Pengfei Tu,Qingying Zhang
Quality analysis of edible mushrooms based on polysaccharides is generally difficult due to their complicated structures and hard separation. Here, multiple fingerprint analysis of polysaccharides based on chromatographic and spectrometric techniques were developed, and then applied in comparative analysis of Auricularia heimuer (AH), Auricularia cornea (AC), Auricularia cornea ʻYu Muerʼ (ACY) and Tremella fuciformis (TF). Firstly, polysaccharides were obtained with the molecular weights between 1.783 × 10 6 and 6.774 × 10 6 Da. Then, complete hydrolysis by TFA and enzyme digestion by cellulase were employed and subsequently analyzed by HPLC-UV, GC–MS, HILIC-HPLC-ELSD and HILIC-HPLC-ESI − -HCD-MS/MS, and ATR-FT-IR were used to characterize the functional groups of intact polysaccharides. By chemometric analysis, differential markers of d -xyl, l -fuc, l -arb, d -glc, disaccharide and hexasaccharide were selected, and AC and ACY were proved to be same species from the viewpoint of polysaccharides firstly. Furthermore, the structures of oligomers with DPs of 2–8 and →4)- β - d -Glc p -(1→ unit with different contents were inferred by combinatory analysis of ESI − -MS/MS, glycosidic linkage, monosaccharide compositions and functional groups. In conclusion, the combinatory method of multiple fingerprint and pattern recognition is powerful not only for structural elucidation of polysaccharides, but also for quality analysis and species differentiation of edible mushrooms from the perspective of biological polysaccharides.
研究领域:
食品科学
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分析化学
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真菌学
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生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）