INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
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2016
·Vol. 17
·期 4
·412
·
IF 3.257
Ning Zhang,Haitao Chen,Baoguo Sun,Xueying Mao,Yuyu Zhang,Ying Zhou
To compare the volatile compounds of Chinese black truffle and white truffle from Yunnan province, this study presents the application of a direct solvent extraction/solvent-assisted flavor evaporation (DSE-SAFE) coupled with a comprehensive two-dimensional gas chromatography (GC × GC) high resolution time-of-flight mass spectrometry (HR-TOF/MS) and an electronic nose. Both of the analytical methods could distinguish the aroma profile of the two samples. In terms of the overall profile of truffle samples in this research, more kinds of acids were detected via the method of DSE-SAFE. Besides, compounds identified in black truffle (BT), but not in white truffle (WT), orvice versa, and those detected in both samples at different levels were considered to play an important role in differentiating the two samples. According to the analysis of electronic nose, the two samples could be separated, as well.Keywords:GC × GC/HR-TOF/MS;electronic nose;DSE-SAFE;Chinese truffles
研究领域:
分析化学
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食品科学
·
农学
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DOI
PubMed
高影响因子
DRUG DELIVERY
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2022
·
IF 6.819
Luxi Peng,Jiajun Qiu,Lidan Liu,Xiaoyu Li,Xuanyong Liu,Yongjun Zhang
Melanoma is one of the highly malignant tumors whose incidence and fatality rates have been increased year by year. However, in addition to early surgical resection, there still lacks specific targeted drugs and treatment strategies. In this study, it was discovered that hinokiflavone (HF) encapsulated in zeolitic imidazolate framework-8 (ZIF-8) exhibited a superior anti-melanoma effect in vitro and in vivo. HF was encapsulated in ZIF-8 through a one-step synthesis method, and polyethylene glycol (PEG-2000) was used to optimize the size and dispersion of the drug-loaded complex (PEG/ZIF-8@HF). The results show that the prepared PEG/ZIF-8@HF has a high encapsulation efficiency (92.12%) and can achieve selective drug release in an acidic microenvironment. The results of in vitro anti-melanoma experiments indicate that PEG/ZIF-8@HF shows up-regulation of reactive oxygen species (ROS) levels and can restrain the migration and invasion of B16F10 cells. Moreover, in vivo animal experiments further confirm that PEG/ZIF-8@HF shows anti-tumor effect by up-regulating the pro-apoptotic proteins caspase-3 and caspase-8, and down-regulating the migration-promoting invasion protein MMP-9. This study developed a safe and effective oral administration of HF based on the high-efficiency delivery ZIF-8 system, which provides an effective treatment strategy for melanoma.
研究领域:
肿瘤学
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药剂学
·
生物化学
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产品关联
DOI
PubMed
高影响因子
ACS Food Science & Technology
·
2025
·
IF 2.8
Lin Yuan,Yiye Luo,Shuo Zhang,Suxuan Li,Zhichao Zhang,Xiaoyu Chen,Yingjie Xu,Lusi Ouyang,Zhiyong Wu
To enhance the aroma complexity and commercial potential of Chinese black truffle (Tuber indicum), enzymatic hydrolyzates (EH) were subjected to Maillard reaction (MR) with xylose/cysteine under varying temperatures (90–130 °C). Volatile organic compounds (VOCs) in MR products (MRPs) were characterized using an E-nose, HS-SPME-GC-MS, and HS-GC-IMS coupled with chemometrics (PCA, OPLS-DA). MR significantly increased VOC diversity (216 compounds identified vs 82 in EH), with sulfur-containing compounds and heterocyclics emerging exclusively in MRPs. Temperature critically influenced VOC profiles: 130 °C (MRPs130) yielded the highest VOC abundance and key odorants, as confirmed by the odor activity value (OAV) analysis. Sankey diagrams visualized a flavor shift from mushroom/earthy notes in EH to enhanced fruity, green, and roasted nuances in MRPs130. This strategy effectively improves the Chinese truffle aroma, offering theoretical and practical insights for value-added processing.
研究领域:
食品科学
·
香气化学
·
食品加工
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代谢组学
·
分析化学
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天然产物化学
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产品关联
DOI
高影响因子
Journal of Food Measurement and Characterization
·
2025
·
IF 3.3
Wu Yi,Hu Hui,Yang Guangzhen,Chen Yifan,Zhong Xiaoyu,Li Shanshan,Pan Ming,Li Yuanbin,Zhang Jiaheng
Natural deep eutectic solvents (NaDESs) with ultrasound-assisted (UAE) method was used to improve the extraction efficiency of polysaccharide from black truffles, which can overcome the limitations of conventional methods. It demonstrated that the extraction efficiency of polysaccharide by six NaDESs with UAE were better than those of conventional solvents. Among the NaDESs, betaine-citric acid (BetCi) NaDES showed the best performance, achieving a remarkable extraction yield of 9.38%, outperforming water, ethanol, and 1,3-butanediol by 0.4, 11.5 and 11.0 times, respectively. Moreover, the polysaccharide yield was further optimized by response surface method, based on three influence factors (NaDES concentration, liquid-to-solid, and ultrasonic time). Scanning electron microscopy images showed that compared to the traditional extraction methods, the plate-like structure fractured into smaller pieces of black truffles treated by BetCi NaDES with UAE, indicating deeper interactions between NaDES and black truffles. Furthermore, the skin penetration as well as antioxidant and anti-wrinkle properties of the black truffle polysaccharide extract were evaluated, revealing promising bioactivity. Overall, this study represents a comprehensive exploration of novel extraction methods, highlighting efficient polysaccharide extraction and its potential applications.
研究领域:
食品科学
·
化学工程
·
药学
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生物技术
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产品关联
DOI
高影响因子
Food Chemistry-X
·
2025
·
IF 8.2
Yiye Luo,Lusi Ouyang,Shuo Zhang,Yusheng Wang,Pingzhang Yao,Yin Liu,Xiaoyu Chen,Lin Yuan,Zhiyong Wu
This study investigated volatile organic compound (VOC) variations across commercial grades (G1, G2, G3) of Chinese black truffles ( Tuber indicum ) using electronic nose ( E -nose), headspace solid-phase microextraction gas chromatography–mass spectrometry (HS-SPM E -GC–MS), and headspace gas chromatography-ion mobility spectrometry (HS-GC-IMS). E-nose analysis revealed significant differences in sulfur-containing and terpenic compounds between grades. HS-SPME-GC–MS identified 95 VOCs, with G1 exhibiting the highest diversity (75 compounds) and unique markers like 2-butanone. Orthogonal partial least squares-discriminant analysis (OPLS-DA) highlighted 86 discriminatory VOCs (VIP > 1). Odor activity value (OAV) analysis showed G1 had the strongest aroma intensity, driven by esters (ethyl isobutyrate, OAV = 6574) and alcohols (1-octen-3-ol, OAV = 3444). Higher-grade truffles (G1) contained distinct sulfur compounds absent in lower grades. This multi-technique approach provides a biochemical basis for refining truffle grading standards beyond morphological criteria.
研究领域:
食品科学
·
分析化学
·
食品化学
·
真菌学
·
挥发性有机物分析
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感官分析
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化学计量学
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2021
·
IF 6.953
Jinling Zheng,Tingting Zhang,Jian Fan,Yongliang Zhuang,Liping Sun
A polysaccharide from the aqueous extract of Boletus aereus fruit (BAP) was isolated. The antitumor activities of BAP and/or cyclophosphamide (CTX) were investigated using the model of S180 tumor-bearing mice. Results indicated that BAP could effectively inhibit the growth of S180 solid tumors and protect the immune organs. Hematoxylin and eosin staining, Annexin V-FITC/PI staining, and mitochondrial membrane potential analysis demonstrated that BAP could induce the apoptosis of S180 tumor cells. In combination with CTX, BAP exhibited a significant synergistic antitumor effect on S180 cells. Furthermore, a novel polysaccharide, namely, BAPF, was purified from BAP by using DEAE Cellulose-52 column and Sephadex G-100 gel column. Structural characterization revealed that BAPF was primarily composed of mannose, glucuronic acid, glucose, galactose, arabinose, and fucose at a proportion of 12.98:1:16.8:16.48:1.08:9.1. Its average molecular weight was 1.79 × 10 6 Da. FTIR and NMR analyses demonstrated that BAPF was a pyranose with α-type and β-type glycosidic residues.
研究领域:
药理学
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肿瘤学
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免疫学
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分子生物学
·
化学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）