Frontiers in Nutrition
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2025
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IF 5.1
Xi Che,Yifei Zhao,Yuxiao Wang,Xin Sun,Hongxun Tao,Rentang Zhang
Introduction: Obesity, a growing public health issue, is closely associated with excessive energy intake and gut microbiota imbalances. Despite the increasing attention given to black jujube as a blackened fermented food in recent years, the role of its melanoidins (MLD) in obesity-related biological mechanisms has yet to be thoroughly investigated.Methods: This study investigated the effects of black jujube MLD on obesity induced by a high-fat diet in C57BL/6J mice. We hypothesize that MLD exerts an anti-obesity effect, potentially mediated by alterations in gut microbiota composition and the modulation of metabolic responses.Results: The results demonstrate that MLD administration mitigated HFD-induced weight gain, improved insulin resistance, and enhanced glucose metabolism by reducing blood glucose and insulin levels (p < 0.05). MLD also protected the liver, as evidenced by a 16.4% decrease in alanine aminotransferase (ALT) levels and a 29.6% decrease in aspartate aminotransferase (AST) levels (p < 0.05). Furthermore, MLD treatment restored the compromised intestinal microbiota to a healthier state at the genus level by lowering the Firmicutes/Bacteroidetes ratio (38.9%), suppressing the abundance of pathogenic bacteria, such as Erysipelatoclostridium and Bacteroides descendants, and promoting the growth of beneficial bacteria, including Bifidobacterium and norank_f_Muribaculaceae. Metabolomic profiling revealed that MLD can alleviate metabolic disorders by downregulating arginine metabolism and sphingolipid metabolism. Its metabolites are significantly correlated with several bacterial genera, including Bacteroides, Roseburia, Erysipelatoclostridium, Bacteroides, norank_f_Muribaculaceae, and Lachnospiraceae NK4A136 group.Discussion: Based on these findings, MLD may mitigate obesity and other associated metabolic disorders by modulating the gut microbiome–metabolism axis.
研究领域:
营养学
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微生物学
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代谢组学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Frontiers in Nutrition
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2022
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IF 6.59
Zhang Guifeng,Liu Chuang,Zhang Rentang
Liver cancer is one of the most common cancers, with increasing trends in incidence and mortality. A novel acidic polysaccharide (BJP-2) obtained from blackened jujube was extracted by hot water followed by chromatographic purification employing DEAE-cellulose 52 and Sephadex G-100 column. And then BJP-2 was identified by SEC-MALLS-RI, GC-MS, methylation and NMR for the following characteristics: molecular weight of 6.42 × 104 Da, monosaccharide composition of glucuronic acid (GalA), arabinose (Ara), galactose (Gal), rhamnose (Rha), xylose (Xyl), glucuronic acid (GlcA), glucose (Glc), fucose (Fuc) and mannose (Man) with the percentage of 39.78, 31.93, 16.86, 6.43, 1.86, 1.28, 1.02, 0.61, and 0.23%, as well as the main chain of → 5)-α-L-Araf (1 → 4)-β-D-Gal(1 → , T-α-L-Araf (1 → 4)-β-D-Gal(1 → , and → 4)-α-L-6MeGalAp(1 → . The effect of BJP-2 on the apoptosis of HepG2 cells and its anti-tumor mechanism were further explored. The analysis by MTT and flow cytometry showed that BJP-2 suppressed cell proliferation by inducing apoptosis in a concentration-dependent manner. Cell scratching and Transwell revealed that BJP-2 was able to block the invasion and metastasis of tumor cells. Western blot results demonstrated that BJP-2 exhibited antitumor activity through a mitochondria-dependent pathway, as evidenced by overexpression of Bax, Cleaved Caspase-3/Caspase-3 and Cleaved Caspase-9/Caspase-9 and downregulation of Bcl-2. Therefore, BJP-2 has broad research prospects as a tumor preventive or therapeutic agent.
研究领域:
食品科学
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肿瘤学
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分子生物学
✓ 已验证
DOI
PubMed
高影响因子
FOOD CHEMISTRY
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2022
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IF 9.231
Chuang Liu,Zhichang Qiu,Duanyin Gu,Fangzhou Wang,Rentang Zhang
This study explored the structural characteristics of the purified blackened polysaccharide fraction (BJP-4) and its potential to alleviate colitis. BJP-4 had a molecular weight of 1.24 × 10 5 Da and mainly consisted of → 4)- α - l -Gal p A (1→, →5)- α - l -Ara f (1 → and → 4)- β - d -Gal p (1 → residues. After the administration of BJP-4, the disease activity index of colitic mice was reduced by 40.38–50.96%, accompanied by increased colon length (30.00–62.50%) and ameliorated pathological damage. The mechanisms involved regulation of the intestinal microbiota and subsequent inhibition of the TLR4/MyD88/NF- κ B/NLRP3 pathway, balance of pro/anti-inflammatory cytokines and oxidative status, enhancement of epithelial barrier integrity and production of short-chain fatty acids. In particular, high-doses of BJP-4 significantly increased the abundance of beneficial bacteria (f_ Muribaculaceae , Lactobacillus and Lachnospiraceae _NK4A136_group, 0.78–1.04-fold) and suppressed the levels of harmful bacteria (o_ Clostridia _UCG-014, Bacteroides and Escherichia-Shigella , 32.30–90.42%). These findings revealed the anti-inflammatory effects and mechanisms of purified blackened polysaccharides, which facilitated their application in functional foods.
研究领域:
药理学
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免疫学
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微生物学
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营养学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）