WEED SCIENCE
·
2025
·
IF 2.5
Shaojing Yin,Guangtao Zhu,Yiheng Li,Liyao Dong,Zhike Feng
Italian ryegrass [Lolium perenne L. ssp. multiflorum (Lam.) Husnot], a pernicious weed in wheat fields, has evolved severe resistance to the acetolactate synthase (ALS)-inhibiting herbicides, like pyroxsulam. Here, the derived cleaved amplified polymorphic sequence (dCAPS) markers were developed to detect two distinct mutations at the 197th position of the ALS gene. The method was used to examine and purify the resistant population. Homozygous populations with different 197 site mutations from the same population were obtained, and the target-site resistance mechanisms were investigated. Whole-plant dose-response bioassays show that the resistance index (RI) of the Pro-197-Thr mutant population to pyroxsulam was 508.92, whereas that of the Pro-197-Gln population was 9.75. Similar trends were observed for different herbicides within same mode of action. In vitro ALS assays demonstrated that the Pro-197-Thr population exhibited lower sensitivity to pyroxsulam than the Pro-197-Gln population, consistent with plant bioassays. Furthermore, ALS gene expression of the Pro-197-Thr population analysis is significantly higher than that in the Pro-197-Gln population which may also explain why the Pro-197-Thr population exhibits higher resistance level than the Pro-197-Gln population. Our findings suggest that different amino acid substitutions at one ALS gene locus can confer herbicide resistance with different levels in L. perenne ssp. multiflorum. This study provides valuable insights into the mechanisms of herbicide resistance in L. perenne ssp. multiflorum.
研究领域:
植物学
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植物科学
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杂草科学
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分子生物学
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遗传学
·
农学
✓ 已验证
产品关联
DOI
高影响因子
Journal of Environmental Chemical Engineering
·
2022
·
IF 7.968
Guangyu Shi,Jiayuan Hu,Shuhan Zhang,Gang Ni,Weilin Shi,Chengxiao Hu,Xiaohu Zhao
Nonylphenol (NP) and cadmium (Cd), very toxic to microorganisms , plants, and animals, are often detected simultaneously in soil and water. Selenium (Se) is important to microorganisms and plants. A pot experiment was carried out to explore the effect of Se on the efficiency of ryegrass in the remediation of NP-Cd co-contaminated soil. The removal rate of soil NP and Cd increased by 6.2 % and 30.8 %, respectively, with the application of exogenous Se in NP-contaminated soil (50 mg·kg −1 ). The selenium significantly increased the catalase activity (11.5–24.5%), but decreased the activities of acid phosphatase (5.5–9.0 %), invertase (19.8–22.5 %), and urease (11.1–18.4 %) in NP contaminated soil (50 mg·kg −1 ) ( p ＜0.05). The selenium also increased the relative abundance of beneficial bacteria, including Massilia , Burkholderiaceae , Micrococcales, and Sphingomonas in the NP-Cd co-contaminated soil. These bacteria are closely related to Cd and NP pollution remediation. In summary, applying Se to the contaminated soil effectively improved the efficiency of the phytoremediation of NP and Cd, and also increased the relative abundance and diversity of microorganisms in the NP-Cd co-contaminated soil. These findings can attract practical interest in phytoremediation measures of organic and heavy metals co-contaminated soil.
研究领域:
环境科学
·
微生物学
·
植物学
✓ 已验证
产品关联
DOI
高影响因子
Environmental Science-Nano
·
2024
·Vol. 11
·期 7
·3019-3032
·
IF 5.8
Jianyu Gong,Ruiyuan Xue,Yake Xu,Hao Lv,Nan Wang,Xiaoming Su
In this research, a bimetallic compound of silver-modified nano zero-valent iron (Ag/Fe0) was synthesized and utilized to degrade pentabromophenyl ether (BDE209) under various environmental conditions with the presence of citric acid (CA). Liquid chromatography-mass spectrometry (LC-MS) analysis revealed twelve intermediate products formed during the degradation of BDE209. Both the Fe0–CA system and 0.2% Ag/Fe0–CA system showcased a degradation efficiency of 68.7% and 72.7% respectively in a liquid solution after 24 hours of treatment, along with a 9.1% and 18.2% total organic carbon (TOC) removal. Amid soil conditions, degradation rates reached 66.4%, 54.1%, and 50.5% respectively for initial BDE concentrations of 5 ppm, 10 ppm and 15 ppm in the presence of a degradation system comprising 30 g kg−1 Ag/Fe0 and sodium citrate/CA (NaCA/CA) over 45 days. The potential environmental impacts of this process were assessed using ryegrass as a phytotoxicity marker in soil, wherein the measured chlorophyll and malondialdehyde (MDA) contents indicated that the addition of Ag/Fe0 successfully mitigated stress damage caused by BDE209. Furthermore, Ag/Fe0 has the capability to permeate the cell wall of ryegrass roots and establish interconnections. STEM-EDS patterns revealed that Ag was more readily accumulated inside of the cells compared to Fe. In conclusion, this study introduces an innovative approach for the safe and efficient oxidative degradation of BDE209 under both aquatic and terrestrial environmental conditions.
研究领域:
环境科学
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植物学
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毒理学
✓ 已验证
产品关联
DOI
高影响因子
Eco-Environment & Health
·
2025
·
IF 17.6
Chunmei Ran,Meiqi Guo,Yuan Wang,Ye Li,Jiao Wang,Yinqing Zhang,Chunguang Liu,Bridget A. Bergquist,Chu Peng
Evaluating the plant risk of soil pollution by plant physiological indices usually requires a long cycle and has significant uncertainty. In this study, oxidative potential (OP) of the in situ heavy metal contaminated soils was measured by the dithiothreitol method. The oxidative stress response of the model plant ryegrass ( Lolium perenne L. ) induced by heavy metal contaminated soil was evaluated by the biomarkers, including superoxide dismutase and total antioxidant capacity. The comprehensive biomarker response index has a significant exponential correlation with the OP of soil ( r = 0.923, p < 0.01) in ryegrass. Metabolomics analysis also showed a significant relationship of the metabolic effect level index of amino acids and sugars with OP. Random forest was selected from four machine learning models to screen the metabolites most relevant to OP, and Shapley additive explanations analysis was used to explain the contribution and the influence direction of the features on the model. Based on the selected 20 metabolites, the metabolic pathways most related to OP in plants, including alkaloid synthesis and amino acids metabolism, were identified. Compared to the plant physiological indices, OP is a more stable and faster indicator for the plant risk assessment of heavy metals contaminated soil.
研究领域:
植物生理学
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环境毒理学
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分析化学
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生物信息学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2016
·Vol. 23
·期 20
·20672-20679
·
IF 2.76
Chen Xiao,Li Hongbing,Liu Xiaoyan,Zhang Xinying,Liang Xia,He Chiquan,Cao Liya
The in situ chemical oxidation technology (ISCO) and phytoremediation for PAHs have been studied respectively, but few focus on the feasibility of combining persulfate with ryegrass. This literature revealed the effect of persulfate oxidation on the growth of ryegrass and the removal ratios of pyrene in the couple system of persulfate oxidation and phytoremediation. The results demonstrated that half of pyrene in test soil was oxidized by persulfate in 7 days and then the residual pyrene concentration was decreased to a lower level by ryegrass in the following 2 months in oxidation treatment and drip washing and plants (OWP) and oxidation treatment and drip washing and plants and fertilization (OWFP) treatment. Ryegrass could grow well after persulfate oxidation with the oxidized soil washed by water. Ryegrass in OWP and OWFP treatments had higher ratios of overground and underground biomass. However, the seeds of ryegrass cannot germinate when drip washing was omitted. Pyrene together with residual persulfate changed soil enzyme activities. Drip washing and the growth of ryegrass made soil enzyme activities tend to returned to normal levels. Persulfate oxidation and phytoremediation were compatible to make contributions to the dissipation of pyrene. Persulfate oxidation activated by heat had higher removal efficiency of PAHs and phytoremediation could further decrease the pyrene concentration in spiked soil.
研究领域:
环境科学
·
土壤修复
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF SOILS AND SEDIMENTS
·
2021
·Vol. 22
·期 3
·797-808
·
IF 3.308
Ma Li,Yao Lei,Li Yuhong
Purpose Bioremediation experiments of polycyclic aromatic hydrocarbon (PAHs) using ryegrass ( Lolium perenne ), a turfgrass in urban landscapes, were carried out aiming to explore the ryegrass-assisted degradation in an actual PAH-contaminated urban soil and the phytotransformation pathways of PAHs within plants. Methods Dynamics of plant-assisted degradation of 15 PAHs in pot soils were recorded over 100-day cultivation, and at the end, the accumulation of PAHs in ryegrass was investigated. Ryegrass with phenanthrene exposure was cultured hydroponically to investigate the phytotransformation intermediates using gas chromatography–mass spectrometry and ultra-performance liquid chromatography coupled to high-resolution mass spectrometry. Results Overall, dissipation of PAHs in soils exhibited an initial fast and subsequent slow degradation pattern. Eventually, on average, 55.7% and 47.1% of \(\sum {PAHs}\) were removed in the planted and unplanted soils, respectively, with the high-molecular-weight PAHs highly eliminated particularly. Plant growth resulted in 4.8–11.1% more \(\sum {PAHs}\) removed from soil compared to the plant-free controls. The PAHs all exhibited the bioconcentration and translocation factors below 1. The mass balance calculation showed that plant uptake accounted for < 1% of the dissipation increase while plant-promoted microbial biodegradation made the dominant contribution. A total of 7 metabolites were tentatively identified based on which possible transformation pathways were proposed. Conclusion The results indicated that ryegrass may act as a metabolic sink for aqueous PAHs while microbes play a dominant role in soil PAH dissipation via biodegradation. The results may pave the path for a scientific, safe, and effective implementation of landscape configuration to ameliorate urban soil pollution.
研究领域:
环境科学
·
植物学
✓ 已验证
产品关联
DOI
高影响因子
BULLETIN OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY
·
2025
·Vol. 115
·期 3
·1-7
·
IF 2.2
Wang Yun,Shan Ning,Habibul Zumrat,Habibul Nuzahat
This study investigated the impact of pH on the combined toxicity of polystyrene microplastics (MPs) and ciprofloxacin (CIP) on ryegrass growth and CIP uptake. Two different sizes of PS-COOH (200 and 500 nm) were utilized. The findings indicated that neutral and alkaline pH conditions (pH 7.0 and 8.8) significantly reduced CIP accumulation in ryegrass roots, regardless of MP presence. Conversely, CIP content in shoots (including leaves) increased with rising pH, showing 24.7-fold and 29.5-fold enhancements under CIP + 200 nm MPs and CIP + 500 nm MPs treatments at pH 8.8 compared to pH 5.3. Additionally, pH significantly influenced growth inhibition, with more pronounced effects at pH 7.0 and 8.8 than at pH 5.3. Under neutral and alkaline conditions, shoot lengths in 500 nm MPs + CIP and 200 nm MPs + CIP treatments were reduced to 50.3%, 69.7% and 86.8%, 81.3% of those at pH 5.3, respectively. These findings highlight the critical role of pH in modulating CIP uptake and toxicity in ryegrass, particularly in MP co-contaminated environments. Moreover, the relatively high CIP removal efficiency suggests the potential of ryegrass for CIP remediation in MP-polluted systems.
研究领域:
环境毒理学
·
植物生理学
·
污染生态学
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土壤生物学
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药物代谢
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环境化学
·
生态风险评估
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Nano
·
2023
·
IF 17.1
Dong Liu,Shahid Iqbal,Heng Gui,Jianchu Xu,Shaoshan An,Baoshan Xing
To understand microplastic–nanomaterial interactions in agricultural systems, a randomized block 90-day pot experiment was set up to cultivate ryegrass seedings in a typical red sandy soil amended with compost (1:9 ratio). Polyvinyl chloride (PVC) and polyethylene (PE) microplastic (MP) contaminants were added into pot soils at 0.1 and 10%, whereas nano-Fe3O4 (as nanoenabled agrochemicals) was added at 0.1% and 0.5% in comparison with chemical-free controls. The combination of nano-Fe3O4 and MPs significantly increased the soil pH (+3% to + 17%) but decreased the total nitrogen content (−9% to – 30%; P < 0.05). The treatment group with both nano-Fe3O4 and PE had the highest total soil C (29 g kg–1 vs 20 g kg–1 in control) and C/N ratio (13 vs 8 in control). Increased rhizosphere nano-Fe3O4 concentrations promoted ryegrass growth (+42% dry weight) by enhancing the chlorophyll (+20%) and carotenoid (+15%) activities. Plant leaf and root peroxidase enzyme activity was more significantly affected by nano-Fe3O4 with PVC (+15%) than with PE (+6%). Nano-Fe3O4 significantly changed the ryegrass bacterial community structure from belowground (the rhizoplane and root endosphere) to aboveground (the phylloplane). Under MP contamination, the addition of nano-Fe3O4 increased bacterial diversity (+0.35%) and abundance (+30%) in the phylloplane and further intensified the connectivity of ryegrass aboveground bacterial networks (positive association increased 17%). The structural equation model showed that the change in the plant microbiome was associated with the rhizosphere microbiome. Overall, these findings imply the positive influences of nano-Fe3O4 on the soil–microbe–plant system and establish a method to alleviate the harmful effects of MP accumulation in soils.
研究领域:
环境科学
·
农业科学
·
微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2022
·Vol. 30
·期 10
·28247-28258
·
IF 5.19
Shi Guangyu,Hu Jiayuan,Cheng Yuanyuan,Shi Weilin,Chen Yan
The effect of Pseudomonas aeruginosa ( P. aeruginosa ) on the phytoremediation efficiency of ryegrass on soil contaminated with nonylphenol (NP) and cadmium (Cd) was investigated by pot experiments. Pseudomonas aeruginosa application stimulated the adsorption of Cd by ryegrass and facilitated the biodegradation of NP in the soil. Exogenous P. aeruginosa inoculation increased the activities of urease, dehydrogenase, and polyphenol oxidase in the soil of the T4 treatment by 38.5%, 50.0%, and 56.5% compared to that of the T2 treatment, respectively. There was a significant positive correlation between the activities of dehydrogenase and polyphenol oxidase and the NP removal rate ( P < 0.001). The relative abundances of beneficial microorganisms (such as Sphingomonas , Lysobacter , Streptomyces , Chloroflexia, Deltaproteobacteria, and Alphaproteobacteria) were increased as a result of P. aeruginosa inoculation. These microorganisms play important roles in nutrient cycling, Cd adsorption, and NP degradation. Additionally, P. aeruginosa was not the dominate bacterial species at the end of the experiment. According to this study, P. aeruginosa application improved the phytoremediation efficiency of ryegrass on soil contaminated with NP and Cd, with a minimal risk of alien microbial invasion.
研究领域:
环境科学
·
微生物学
·
土壤学
·
生态学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Agronomy-Basel
·
2022
·Vol. 12
·期 9
·2203
·
IF 3.949
Xiangyang Qin,Cheng Yang,Mengmeng Hu,Yunxia Duan,Na Zhang,Jinxin Wang,Hengzhi Wang,Weitang Liu
Black-grass (Alopecurus myosuroidesHuds.) is a common weed in Chinese wheat fields, and has become troublesome due to its evolution of herbicide resistance. One black-grass population (HN-14) collected from a wheat field where herbicides were applied was suspected to be resistant (R) to mesosulfuron-methyl. This study aims to establish a cross-resistance pattern and explore potential resistance mechanisms. The results of a whole-plant dose response assay showed that the resistant (R) population had a high of resistance to mesosulfuron-methyl (33-fold); meanwhile, no synergism of P450s activity inhibitor malathion was observed. The sequencing results revealed that ALS resistance mutation Trp-574-Leu occurred in R plants. The results of in vitro ALS enzyme activity assays also supported that the extractable ALS from R plants were 23.22-fold resistant to mesosulfuron-methyl. In the light of the “R” resistance rating system, HN-14 has evolved RRR and RR resistance to fenoxaprop-P-ethyl, clodinafop-propargyl, haloxyfop-methyl, and fluazifop-P-butyl and R? (resistance may be developing) to pinoxaden, however remains sensitive to imazethapyr, quizalofop-P-ethyl, tralkoxydim, and isoproturon. These results indicated that the mesosulfuron-methyl resistance in the black-grass population HN-14 was driven by a target-site mechanism rather than a nontarget (at least P450s-mediated) mechanism.Keywords:herbicide resistance mechanism;cross resistance;acetolactate synthase (ALS) inhibitor;Alopecurus myosuroideshuds
研究领域:
植物学
·
植物科学
·
分子生物学
·
杂草科学
✓ 已验证
产品关联
DOI
高影响因子
ENVIRONMENTAL POLLUTION
·
2021
·
IF 8.071
Gang Ni,Guangyu Shi,Chengxiao Hu,Xu Wang,Min Nie,Miaomiao Cai,Qin Cheng,Xiaohu Zhao
Most chemical plant wastewater contains both organic and inorganic pollutants , which are easy to diffuse along with surface runoff. The combined pollution of nonylphenol (NP) and cadmium (Cd) in soil is a serious problem that has not attracted enough attention. Based on the effects of selenium (Se) and Pseudomonas aeruginosa ( P. aeruginosa ) on plant and soil microbial communities , we speculated that the application of Se and P. aeruginosa in soil could improve the phytoremediation efficiency of ryegrass on contaminated soil. In this study, pot experiments with Cd and NP co-contaminated soil were conducted, and the results showed that application of P. aeruinosa alone could improve the removal rates of NP and Cd by ryegrass, and the supplementary of Se further enhanced the effect of micro-phyto remediation, with the highest removal rates of NP and Cd were 79.6% and 49.4%, respectively. The application of P. aeruginosa plus Se reduced the adsorption of Cd and NP through C–O and Si–O–Fe of the soil, changed the enzyme activity , and also affected the changing trend of the microbial community in soil. Pseudomonas , Sphingomonadales , Nitrospira , and other beneficial bacteria were enriched after a 60-day period with P. aeruginosa and Se treatment, thus promoting the removal of NP and Cd. In light of the above results, we suggest that P. aeruginosa application can efficiently facilitate the phytoremediation of ryegrass on Cd-NP co-contaminated soil, and Se supplementation in soil showed the synergistic effect on the remediation.
研究领域:
环境科学
·
土壤学
·
微生物学
·
植物科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Chemical and Biological Technologies in Agriculture
·
2025
·Vol. 12
·期 1
·1-14
·
IF 5.2
Sha Muzhi,Wang Yunlong,Diao Junpeng,Savaldi-Goldstein Sigal,Wang Qian
Background Vivianite is an intensively studied phosphorus (P) recovery product from wastewater treatment plants. However, its downstream application has not been well-addressed thus restricting P cycling. This study investigated the P fertilizer efficiency of vivianite in pot experiments using ryegrass ( Lolium multiflorum L.) and tomato ( Solanum lycopersicum L.) with two soilless growing media (perlite and quartz). The variation of vivianite during fertilization was examined to elucidate the mechanisms of P release from vivianite, using a recently developed sequential P extraction protocol coupled with light microscopy, X-ray diffraction (XRD), and scanning electron microscopy (SEM) coupled with energy dispersive X-ray spectroscopy (EDS). Results The mineral fertilizer equivalence (MFE) of vivianite was determined by comparing the P uptake by plants under vivianite treatment to that under mineral P fertilizer treatment, i.e., Ca(H 2 PO 4 ) 2 in this study. It shows that the vivianite-P uptake of ryegrass was positively correlated with time in a 3-month period and its uptake in quartz was faster than that in perlite, leading to a higher MFE of vivianite in quartz than in perlite (15.8–20.3% vs 6.6–7.4%). This should be attributed to the higher P adsorption capacity and lower hydraulic conductivity of perlite, which restricted the vivianite-P dissolution and mobility. The vivianite variation revealed that vivianite-P was mainly released upon vivianite oxidation to ferric (hydr)oxides in perlite, probably given the high aeration condition in this type of growing medium. In quartz, however, the release of vivianite-P was most likely due to vivianite dissolution driven by the plant uptake of P, during which a higher plant uptake of Fe was observed than that in perlite. Conclusions The findings reveal that vivianite can serve as a slow-release P fertilizer, and its fertilizer efficiency is highly related to the properties of the growing media. Quartz is a preferred growing medium over perlite for the efficient fertilization of this sparingly soluble P source. The findings deepen the understanding of P fertilization of vivianite and lay foundation to its utilization as a slow-release P fertilizer for a circular economy of P. Graphical
研究领域:
农学
·
土壤学
·
环境科学
·
植物营养
·
肥料技术
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）