WEED SCIENCE
·
2025
·
IF 2.5
Shaojing Yin,Guangtao Zhu,Yiheng Li,Liyao Dong,Zhike Feng
Italian ryegrass [Lolium perenne L. ssp. multiflorum (Lam.) Husnot], a pernicious weed in wheat fields, has evolved severe resistance to the acetolactate synthase (ALS)-inhibiting herbicides, like pyroxsulam. Here, the derived cleaved amplified polymorphic sequence (dCAPS) markers were developed to detect two distinct mutations at the 197th position of the ALS gene. The method was used to examine and purify the resistant population. Homozygous populations with different 197 site mutations from the same population were obtained, and the target-site resistance mechanisms were investigated. Whole-plant dose-response bioassays show that the resistance index (RI) of the Pro-197-Thr mutant population to pyroxsulam was 508.92, whereas that of the Pro-197-Gln population was 9.75. Similar trends were observed for different herbicides within same mode of action. In vitro ALS assays demonstrated that the Pro-197-Thr population exhibited lower sensitivity to pyroxsulam than the Pro-197-Gln population, consistent with plant bioassays. Furthermore, ALS gene expression of the Pro-197-Thr population analysis is significantly higher than that in the Pro-197-Gln population which may also explain why the Pro-197-Thr population exhibits higher resistance level than the Pro-197-Gln population. Our findings suggest that different amino acid substitutions at one ALS gene locus can confer herbicide resistance with different levels in L. perenne ssp. multiflorum. This study provides valuable insights into the mechanisms of herbicide resistance in L. perenne ssp. multiflorum.
研究领域:
植物学
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植物科学
·
杂草科学
·
分子生物学
·
遗传学
·
农学
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产品关联
DOI
高影响因子
Environmental Science-Nano
·
2024
·Vol. 11
·期 7
·3019-3032
·
IF 5.8
Jianyu Gong,Ruiyuan Xue,Yake Xu,Hao Lv,Nan Wang,Xiaoming Su
In this research, a bimetallic compound of silver-modified nano zero-valent iron (Ag/Fe0) was synthesized and utilized to degrade pentabromophenyl ether (BDE209) under various environmental conditions with the presence of citric acid (CA). Liquid chromatography-mass spectrometry (LC-MS) analysis revealed twelve intermediate products formed during the degradation of BDE209. Both the Fe0–CA system and 0.2% Ag/Fe0–CA system showcased a degradation efficiency of 68.7% and 72.7% respectively in a liquid solution after 24 hours of treatment, along with a 9.1% and 18.2% total organic carbon (TOC) removal. Amid soil conditions, degradation rates reached 66.4%, 54.1%, and 50.5% respectively for initial BDE concentrations of 5 ppm, 10 ppm and 15 ppm in the presence of a degradation system comprising 30 g kg−1 Ag/Fe0 and sodium citrate/CA (NaCA/CA) over 45 days. The potential environmental impacts of this process were assessed using ryegrass as a phytotoxicity marker in soil, wherein the measured chlorophyll and malondialdehyde (MDA) contents indicated that the addition of Ag/Fe0 successfully mitigated stress damage caused by BDE209. Furthermore, Ag/Fe0 has the capability to permeate the cell wall of ryegrass roots and establish interconnections. STEM-EDS patterns revealed that Ag was more readily accumulated inside of the cells compared to Fe. In conclusion, this study introduces an innovative approach for the safe and efficient oxidative degradation of BDE209 under both aquatic and terrestrial environmental conditions.
研究领域:
环境科学
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植物学
·
毒理学
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产品关联
DOI
高影响因子
Eco-Environment & Health
·
2025
·
IF 17.6
Chunmei Ran,Meiqi Guo,Yuan Wang,Ye Li,Jiao Wang,Yinqing Zhang,Chunguang Liu,Bridget A. Bergquist,Chu Peng
Evaluating the plant risk of soil pollution by plant physiological indices usually requires a long cycle and has significant uncertainty. In this study, oxidative potential (OP) of the in situ heavy metal contaminated soils was measured by the dithiothreitol method. The oxidative stress response of the model plant ryegrass ( Lolium perenne L. ) induced by heavy metal contaminated soil was evaluated by the biomarkers, including superoxide dismutase and total antioxidant capacity. The comprehensive biomarker response index has a significant exponential correlation with the OP of soil ( r = 0.923, p < 0.01) in ryegrass. Metabolomics analysis also showed a significant relationship of the metabolic effect level index of amino acids and sugars with OP. Random forest was selected from four machine learning models to screen the metabolites most relevant to OP, and Shapley additive explanations analysis was used to explain the contribution and the influence direction of the features on the model. Based on the selected 20 metabolites, the metabolic pathways most related to OP in plants, including alkaloid synthesis and amino acids metabolism, were identified. Compared to the plant physiological indices, OP is a more stable and faster indicator for the plant risk assessment of heavy metals contaminated soil.
研究领域:
植物生理学
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环境毒理学
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分析化学
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生物信息学
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产品关联
DOI
PubMed
高影响因子
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2016
·Vol. 23
·期 20
·20672-20679
·
IF 2.76
Chen Xiao,Li Hongbing,Liu Xiaoyan,Zhang Xinying,Liang Xia,He Chiquan,Cao Liya
The in situ chemical oxidation technology (ISCO) and phytoremediation for PAHs have been studied respectively, but few focus on the feasibility of combining persulfate with ryegrass. This literature revealed the effect of persulfate oxidation on the growth of ryegrass and the removal ratios of pyrene in the couple system of persulfate oxidation and phytoremediation. The results demonstrated that half of pyrene in test soil was oxidized by persulfate in 7 days and then the residual pyrene concentration was decreased to a lower level by ryegrass in the following 2 months in oxidation treatment and drip washing and plants (OWP) and oxidation treatment and drip washing and plants and fertilization (OWFP) treatment. Ryegrass could grow well after persulfate oxidation with the oxidized soil washed by water. Ryegrass in OWP and OWFP treatments had higher ratios of overground and underground biomass. However, the seeds of ryegrass cannot germinate when drip washing was omitted. Pyrene together with residual persulfate changed soil enzyme activities. Drip washing and the growth of ryegrass made soil enzyme activities tend to returned to normal levels. Persulfate oxidation and phytoremediation were compatible to make contributions to the dissipation of pyrene. Persulfate oxidation activated by heat had higher removal efficiency of PAHs and phytoremediation could further decrease the pyrene concentration in spiked soil.
研究领域:
环境科学
·
土壤修复
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产品关联
DOI
PubMed
高影响因子
Foods
·
2024
·Vol. 13
·期 17
·2724
·
IF 4.7
Hua Li,Hua Li,Yu Liu,Ruixin Liu,Sirithon Siriamornpun
Heat–moisture treatment (HMT) is a widely used method for modifying starch properties with the potential to reduce the digestibility of high-amylose starch (HAS). This study aimed to optimize the HMT conditions for HAS and apply the resulting HMT-HAS to triticale noodles to develop low-glycemic-index products. HMT significantly increased the resistant starch (RS) content and decreased the rapidly digestible starch (RDS) content of HAS. The treatment conditions—temperature, heating time, and moisture content—were found to significantly influence the starch composition. Optimal HMT conditions were determined using response surface methodology: a temperature of 108 °C, a heating time of 5.8 h, and a moisture content of 25.50%. Under these conditions, the RS content of HMT-HAS was 60.23%, nearly double that of the untreated sample. Increasing the level of HMT-HAS in triticale noodles led to significant decreases in short-range order, relative crystallinity, and viscosities, while the RS content increased from 12.08% to 34.41%. These findings suggest that incorporating HMT-HAS into triticale noodles effectively enhances starch digestive resistance, supporting the development of functional, low-glycemic-index triticale-based foods.
研究领域:
食品科学
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营养学
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Teng Zhang,Jialiang He,Ming Xu,Wenqing Shi,Hao Jiang
The present study sought to examine the impact of cold plasma (CP) treatment on the functionality, structural integrity, and bioactive properties of biomolecules such as starch and phenolic compounds in Xinong Black Spike whole flour (XBS-WF). XBS-WF was exposed to CP at 40 V for various durations. X-ray Diffraction (XRD) and Rapid Viscosity Analysis (RVA) revealed that CP treatment induced aggregation and cross-linking of starch and other molecules, leading to enhanced intermolecular interactions and improved starch crystallinity. In addition, Differential Scanning Calorimetry (DSC) showed enhanced thermal stability and increased enthalpy. Furthermore, CP treatment promoted the oxidation and cleavage of phenolic macromolecules into smaller, biologically active compounds, resulting in a notable increase in antioxidant and anticancer activities. These findings suggest that CP treatment can effectively improve the functional and qualitative properties of XBS-WF, particularly by modifying starch and phenolic macromolecules. This study provides a foundation for exploring the multiscale applications of the CP process on starch and phenolic macromolecules.
研究领域:
食品科学
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生物化学
·
功能食品研究
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产品关联
DOI
PubMed
高影响因子
BULLETIN OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY
·
2025
·Vol. 115
·期 3
·1-7
·
IF 2.2
Wang Yun,Shan Ning,Habibul Zumrat,Habibul Nuzahat
This study investigated the impact of pH on the combined toxicity of polystyrene microplastics (MPs) and ciprofloxacin (CIP) on ryegrass growth and CIP uptake. Two different sizes of PS-COOH (200 and 500 nm) were utilized. The findings indicated that neutral and alkaline pH conditions (pH 7.0 and 8.8) significantly reduced CIP accumulation in ryegrass roots, regardless of MP presence. Conversely, CIP content in shoots (including leaves) increased with rising pH, showing 24.7-fold and 29.5-fold enhancements under CIP + 200 nm MPs and CIP + 500 nm MPs treatments at pH 8.8 compared to pH 5.3. Additionally, pH significantly influenced growth inhibition, with more pronounced effects at pH 7.0 and 8.8 than at pH 5.3. Under neutral and alkaline conditions, shoot lengths in 500 nm MPs + CIP and 200 nm MPs + CIP treatments were reduced to 50.3%, 69.7% and 86.8%, 81.3% of those at pH 5.3, respectively. These findings highlight the critical role of pH in modulating CIP uptake and toxicity in ryegrass, particularly in MP co-contaminated environments. Moreover, the relatively high CIP removal efficiency suggests the potential of ryegrass for CIP remediation in MP-polluted systems.
研究领域:
环境毒理学
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植物生理学
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污染生态学
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土壤生物学
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药物代谢
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环境化学
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生态风险评估
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产品关联
DOI
PubMed
高影响因子
Chemical and Biological Technologies in Agriculture
·
2025
·Vol. 12
·期 1
·1-14
·
IF 5.2
Sha Muzhi,Wang Yunlong,Diao Junpeng,Savaldi-Goldstein Sigal,Wang Qian
Background Vivianite is an intensively studied phosphorus (P) recovery product from wastewater treatment plants. However, its downstream application has not been well-addressed thus restricting P cycling. This study investigated the P fertilizer efficiency of vivianite in pot experiments using ryegrass ( Lolium multiflorum L.) and tomato ( Solanum lycopersicum L.) with two soilless growing media (perlite and quartz). The variation of vivianite during fertilization was examined to elucidate the mechanisms of P release from vivianite, using a recently developed sequential P extraction protocol coupled with light microscopy, X-ray diffraction (XRD), and scanning electron microscopy (SEM) coupled with energy dispersive X-ray spectroscopy (EDS). Results The mineral fertilizer equivalence (MFE) of vivianite was determined by comparing the P uptake by plants under vivianite treatment to that under mineral P fertilizer treatment, i.e., Ca(H 2 PO 4 ) 2 in this study. It shows that the vivianite-P uptake of ryegrass was positively correlated with time in a 3-month period and its uptake in quartz was faster than that in perlite, leading to a higher MFE of vivianite in quartz than in perlite (15.8–20.3% vs 6.6–7.4%). This should be attributed to the higher P adsorption capacity and lower hydraulic conductivity of perlite, which restricted the vivianite-P dissolution and mobility. The vivianite variation revealed that vivianite-P was mainly released upon vivianite oxidation to ferric (hydr)oxides in perlite, probably given the high aeration condition in this type of growing medium. In quartz, however, the release of vivianite-P was most likely due to vivianite dissolution driven by the plant uptake of P, during which a higher plant uptake of Fe was observed than that in perlite. Conclusions The findings reveal that vivianite can serve as a slow-release P fertilizer, and its fertilizer efficiency is highly related to the properties of the growing media. Quartz is a preferred growing medium over perlite for the efficient fertilization of this sparingly soluble P source. The findings deepen the understanding of P fertilization of vivianite and lay foundation to its utilization as a slow-release P fertilizer for a circular economy of P. Graphical
研究领域:
农学
·
土壤学
·
环境科学
·
植物营养
·
肥料技术
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产品关联
DOI
高影响因子
PLANT PHYSIOLOGY AND BIOCHEMISTRY
·
2024
·
IF 6.1
Chenghao Huang,Fei Huang,Xuke Wang,Dongru Wang,Jiawei Wang,Xinhua Zhan
Polycyclic aromatic hydrocarbons (PAHs) constitute a category of persistent organic contaminants that possess the potential to induce carcinogenic, teratogenic, and mutagenic consequences. Our previous findings have revealed that plant roots actively take up PAHs through co-transport with protons, and auxin can promote PAHs uptake by wheat roots. It remains unclear whether nitric oxide (NO), a signaling molecule involved in numerous physiological processes in plants and downstream of auxin, can affect PAHs uptake by plant roots. In our study, 50 μmol/L sodium nitroprusside (SNP) significantly enhanced phenanthrene uptake after 4 h of exposure. After the addition of SNP (50 μmol/L), the H + flux on root surface increased, and H + -ATPase activity was activated, indicating that exogenous NO promotes phenanthrene uptake by plant roots via activating H + -ATPase. By studying the effects of 50 μmol/L cyclic guanosine monophosphate (cGMP), 5 mmol/L Ca 2+ , and 50 μmol/L adenosine monophosphate (AMP) on phenanthrene uptake by ryegrass roots and measuring root calcium-dependent protein kinases (CDPK) activity, we demonstrated that exogenous NO promotes phenanthrene uptake through the signaling pathway of NO, cGMP, Ca 2+ , CDPK, 14-3-3 protein and H + -ATPase. The results contribute significant insights into elucidating the underlying mechanisms of NO modulating PAHs absorption by plant roots, thereby offering crucial strategies for advancing food safety measures and enhancing the phytoremediation potential of soils and waters contaminated with PAHs.
研究领域:
环境科学
·
植物生理学
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植物修复
·
土壤污染
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Chunyan Hou,Lianjia Zhao,Muhua Ji,Jingjing Yu,Yan Di,Qian Liu,Zhengmao Zhang,Lijun Sun,Xuebo Liu,Yutang Wang
Phenolics in bound form extensively exist in cereal dietary fiber, especially insoluble fiber, while their release profile in gastrointestinal tract and contribution to the potential positive effects of dietary fiber in modulating gut microbiota still needs to be disclosed. In this work, the composition of bound phenolics (BPs) in triticale insoluble dietary fiber (TIDF) was studied, and in vitro gastrointestinal digestion as well as colonic fermentation were performed to investigate BPs liberation and their role in regulating intestinal flora of TIDF. It turned out that most BPs were unaccessible in digestion but partly released continuously during fermentation. 16 s rRNA sequencing demonstrated that TIDF possessed prebiotic effects by promoting anti-inflammatory while inhibiting proinflammatory bacteria alongside boosting SCFAs production and antioxidative BPs contributed a lot to these effects. Results indicated that TIDF held capabilities to regulate intestinal flora and BPs were important functional components to the health benefits of cereal dietary fiber.
研究领域:
食品科学
·
营养学
·
微生物学
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产品关联
DOI
PubMed
高影响因子
Agronomy-Basel
·
2025
·Vol. 15
·期 8
·1813
·
IF 3.4
Yufei Zhao,Xu Yang,Qiang Hu,Jie Zhang,Sumei Wan,Wen Chen
Asia minor bluegrass (Polypogon fugax), a widespread Poaceae weed, exhibits broad tolerance to abiotic stresses. Validated reference genes (RGs) for reliable RT-qPCR normalization in this ecologically and agriculturally significant species remain unidentified. This study identified eight candidate RGs using transcriptome data from seedling tissues. We assessed the expression stability of these eight RGs across various abiotic stresses and developmental stages using Delta Ct, BestKeeper, geNorm, and NormFinder algorithms. A comprehensive stability ranking was generated using RefFinder, with validation performed using the target genesCOR413andP5CS. Results identifiedEIF4AandTUBas the optimal RG combination for normalizing gene expression during heat stress, cold stress, and growth stages.EIF4AandACTwere most stable under drought stress,EIF4Aand28Sunder salt stress, andEIF4AandEF-1 under cadmium (Cd) stress. Furthermore,EIF4AandUBQdemonstrated optimal stability under herbicide stress. Additionally, application of validated RGs revealed higher acetyl-CoA carboxylase gene (ACCase) expression in one herbicide-resistant population, suggesting target-site gene overexpression contributes to resistance. This work presents the first systematic evaluation of RGs inP. fugax. The identified stable RGs provide essential tools for future gene expression studies on growth and abiotic stress responses in this species, facilitating deeper insights into the molecular basis of its weediness and adaptability.
研究领域:
植物学
·
分子生物学
·
植物遗传学
·
杂草科学
·
胁迫生理学
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产品关联
DOI
高影响因子
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
·
2025
·
IF 4.1
Gang Liu,Aoyang Zhang,Shams Forruque Ahmed,Xiaoming Guo,Weiwu Ma
Capacitive carbon is a critical material for efficient energy storage in supercapacitors, but its conventional synthesis involves complex multi-step procedures and harsh chemical activators. This study proposes an innovative biomass nanoarchitectonics strategy via one-step synthesis using agricultural waste ryegrass straw as carbon precursor and eco-friendly citrates as activator, yielding porous carbon with excellent electrochemical properties. The optimized material, designated RSC-4K-700, is produced by using potassium citrate as the activating agent, with a highly desirable hierarchical pore structure. It features a hierarchical pore structure with abundant micropores and moderate mesopores, while its surface is enriched with 16.65% oxygen and a high proportion of pyrrolic/pyridinic nitrogen species, which enhances electrochemical reactivity. In a 6M KOH electrolyte two-electrode system, RSC-4K-700 achieves a specific capacitance of 216 F/g at 1 A/g. At a high current density of 5 A/g, it retains 96.98% of initial capacitance after 30,000 charge-discharge cycles, showcasing excellent cycling stability. It delivers a high energy density of 7.61 Wh/kg at a power density of 123.5 W/kg. The biomass nanoarchitectonics approach demonstrated here provides a novel method to utilize biomass for preparing high-performance capacitive carbon electrode materials in supercapacitors.
研究领域:
储能
·
材料科学
·
纳米技术
·
电化学
·
可持续化学
·
可再生资源
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）